5 Works

Semi-artificial datasets as a resource for validation of bioinformatics pipelines for plant virus detection

Lucie Tamisier, Annelies Haegeman, Yoika Foucart, Nicolas Fouillien, Maher Al Rwahnih, Nihal Buzkan, Thierry Candresse, Michela Chiumenti, Kris De Jonghe, Marie Lefebvre, Paolo Margaria, Jean Sébastien Reynard, Kristian Stevens, Denis Kutnjak & Sébastien Massart
In the last decade, High-Throughput Sequencing (HTS) has revolutionized biology and medicine. This technology allows the sequencing of huge amount of DNA and RNA fragments at a very low price. In medicine, HTS tests for disease diagnostics are already brought into routine practice. However, the adoption in plant health diagnostics is still limited. One of the main bottlenecks is the lack of expertise and consensus on the standardization of the data analysis. The Plant Health...

Data from: Differential requirements for the RAD51 paralogs in genome repair and maintenance in human cells

Edwige B. Garcin, Stéphanie Gon, Meghan R. Sullivan, Gregory J. Brunette, Anne De Cian, Jean-Paul Concordet, Carine Giovannangeli, Wilhelm G. Dirks, Sonja Eberth, Kara A. Bernstein, Rohit Prakash, Maria Jasin & Mauro Modesti
Deficiency in several of the classical human RAD51 paralogs [RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3] is associated with cancer predisposition and Fanconi anemia. To investigate their functions, isogenic disruption mutants for each were generated in non-transformed MCF10A mammary epithelial cells and in transformed U2OS and HEK293 cells. In U2OS and HEK293 cells, viable ablated clones were readily isolated for each RAD51 paralog; in contrast, with the exception of RAD51B, RAD51 paralogs are cell-essential in MCF10A...

Higher phage virulence accelerates the evolution of host resistance

Carolin Wendling, Janina Lange, Heiko Liesegang, Michael Sieber, Anja Pöhlein, Boyke Bunk, Jelena Rajkov, Olivia Roth & Michael Brockhurst
Pathogens vary strikingly in their virulence and the selection they impose on their hosts. While the evolution of different virulence levels is well studied, the evolution of host resistance in response to different virulence levels is less understood and as of now mainly based on observations and theoretical predictions with few experimental tests. Increased virulence can increase selection for host resistance evolution if resistance costs are outweighed by the benefits of avoiding infection. To test...

Data from: Phylotranscriptomic consolidation of the jawed vertebrate timetree

Iker Irisarri, Denis Baurain, Henner Brinkmann, Frédéric Delsuc, Jean-Yves Sire, Alexander Kupfer, Jörn Petersen, Michael Jarek, Axel Meyer, Miguel Vences & Hervé Philippe
Phylogenomics is extremely powerful but introduces new challenges as no agreement exists on “standards” for data selection, curation and tree inference. We use jawed vertebrates (Gnathostomata) as model to address these issues. Despite considerable efforts in resolving their evolutionary history and macroevolution, few studies have included a full phylogenetic diversity of gnathostomes and some relationships remain controversial. We tested a novel bioinformatic pipeline to assemble large and accurate phylogenomic datasets from RNA sequencing and find...

Data from: The impact of even-aged and uneven-aged forest management on regional biodiversity of multiple taxa in European beech forests

Peter Schall, Martin M. Gossner, Steffi Heinrichs, Markus Fischer, Steffen Boch, Daniel Prati, Kirsten Jung, Vanessa Baumgartner, Stefan Blaser, Stefan Böhm, Francois Buscot, Rolf Daniel, Kezia Goldmann, Kirstin Kaiser, Tiemo Kahl, Markus Lange, Jörg Müller, Jörg Overmann, Swen C. Renner, Ernst-Detlef Schulze, Johannes Sikorski, Marco Tschapka, Manfred Türke, Wolfgang W. Weisser, Bernd Wemheuer … & Kristin Kaiser
For managed temperate forests, conservationists and policymakers favour fine-grained uneven-aged management over more traditional coarse-grained even-aged management, based on the assumption that within-stand habitat heterogeneity enhances biodiversity. There is, however, little empirical evidence to support this assumption. We investigated for the first time how differently grained forest management systems affect the biodiversity of multiple above- and below-ground taxa across spatial scales. We sampled 15 taxa of animals, plants, fungi and bacteria within the largest contiguous...

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  • 2022
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Resource Types

  • Dataset
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Affiliations

  • Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
    5
  • University of Liège
    2
  • University of Göttingen
    2
  • Helmholtz Centre for Environmental Research
    1
  • Institute for Sustainable Plant Protection
    1
  • Technische Universität Braunschweig
    1
  • University of Pittsburgh
    1
  • German Center for Integrative Biodiversity Research
    1
  • University of Freiburg
    1
  • Manchester University
    1