213 Works

Alterations of long non-coding RNA and mRNA profiles associated with extracellular matrix homeostasis and vascular aging in rats

Qianqin Li, Zezhou Xiao, Yongsheng Wang, Ximao Liu, Hao Liu, Zhiwen Luo & Shaoyi Zheng
Vascular aging has been closely associated with various cardiovascular disorders; however, its molecular mechanism remains poorly understood. In our study, RNA sequencing was utilized to explore the expression profiles of long non-coding RNAs (lncRNAs) and mRNAs in the thoracic aortas of young (3 weeks) and old (16 weeks) rats. Functional categorization of differentially expressed mRNAs was evaluated using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases, and lncRNA–microRNA–mRNA networks was constructed using...

The complete chloroplast genome provides insight into the polymorphism and adaptive evolution of Garcinia paucinervis

Yifei Wang, Bo Zhao, Zhaocen Lu, Yancai Shi & Jingjian Li
Garcinia paucinervis is an evergreen tree with high medicinal value. Due to its vulnerable reproductive capacity coupled with excessive logging by humans, G. paucinervis has become an endangered species. In order to protect this species effectively, we drew the complete chloroplast genome of G. paucinervis and performed a series of comparative analyses on G. paucinervis and its neighbouring species. The chloroplast genome size of G. paucinervis is 157702 bp. In the chloroplast genome, we identified...

Naoxintong accelerates diabetic wound healing by attenuating inflammatory response

Leyu Fang, Lu Chen, Min Song, Juan He, Lusha Zhang, Chunxiao Li, Qianyi Wang, Wenjie Yang, Wei Sun, Yuze Leng, Hong Shi, Shaoxia Wang, Xiumei Gao & Hong Wang
Naoxintong (NXT), a prescribed traditional Chinese medicine, widely used in cerebrovascular and cardiovascular diseases, could be effective in diabetic wounds. This study evaluates the wound healing activity of NXT by employing an excisional wound splinting model. NXT was dissolved in saline and given daily by gavage. Wounds were induced at the dorsum of non-diabetic (db/+) and diabetic (db/db) mice and treated with saline or 700 mg/kg/d NXT for 16 days. Wound closure was measured every...

MicroRNA-146a-5p enhances T helper 17 cell differentiation via decreasing a disintegrin and metalloprotease 17 level in primary sjögren’s syndrome

Xiaoyan Wang, Shaojun Xin, Youqing Wang, Duo Ju, Quan Wu, Ye Qiu, Xuemin Niu, Wei Liu, Jianyou Li & Pengtian Ji
In clinical practice, we found that microRNA (miR)-146a-5p is significantly up-regulated in peripheral blood mononuclear cells (PBMCs) of primary sjögren’s syndrome (pSS) patients. In vitro experiments confirmed that miR-146a-5p promotes T helper 17 (Th17) cell differentiation, but the specific mechanism is still unknown. To solve this problem, 20 pSS patients and 20 healthy subjects were enrolled in this study and PBMCs were isolated from their blood. The expression of the membrane IL-23 R (mIL-23 R)...

The lipoprotein NlpD in Cronobacter sakazakii responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity

Xuemeng Ji, Ping Lu, Juan Xue, Ning Zhao, Yan Zhang, Lu Dong, Xuejiao Zhang, Ping Li, Yaozhong Hu, Jin Wang, Bowei Zhang, Jingmin Liu, Huan lv & Shuo Wang
Cronobacter sakazakii, an emerging opportunistic pathogen, is implicated in severe foodborne outbreak infections in premature and full-term infants. Generally, acid tolerance is vital for the pathogenesis of foodborne pathogens; however, its role in C. sakazakii virulence remains largely unknown. To screen out acid-tolerance determinants from transposon mutants, anovel counterselection method using gentamicin and acid was developed. Using the counterselection method and growth assay, we screened several acid-sensitive mutants and found that nlpD encodes an acid-resistance...

The complete chloroplast genome provides insight into the polymorphism and adaptive evolution of Garcinia paucinervis

Yifei Wang, Bo Zhao, Zhaocen Lu, Yancai Shi & Jingjian Li
Garcinia paucinervis is an evergreen tree with high medicinal value. Due to its vulnerable reproductive capacity coupled with excessive logging by humans, G. paucinervis has become an endangered species. In order to protect this species effectively, we drew the complete chloroplast genome of G. paucinervis and performed a series of comparative analyses on G. paucinervis and its neighbouring species. The chloroplast genome size of G. paucinervis is 157702 bp. In the chloroplast genome, we identified...

T-box transcription factor TBX1, targeted by microRNA-6727-5p, inhibits cell growth and enhances cisplatin chemosensitivity of cervical cancer cells through AKT and MAPK pathways

Haixia Liu, Mei Song, Xiaoyan Sun, Xin Zhang, Huayan Miao & Yankui Wang
Cervical cancer (CC) is the fourth most common cancers among women worldwide. T-box transcription factor 1 (TBX1), a member of the T-box family, has anti-tumor effects in some types of cancer, but its role in CC is yet unknown. The aim of this study is to investigate the functions and underlying mechanisms of TBX1 in CC. Online database UALCAN showed that TBX1 was down-regulated in CC tissues compared with normal tissues and patients with lower...

Modeling results of seismoelectric signals excited by a weight-drop source

Shichuan Yuan, Hengxin Ren, Qinghua Huang, Xu-Zhen Zheng, Zhentao Yang, Zhanxiang He, Wei Zhang & Xiaofei Chen
This dataset contains data of all figures in a submitted manuscript titled "Refining higher modes of Rayleigh waves using seismoelectric signals excited by a weight-drop source: study from numerical simulation aspect " to Journal of Geophysical Research: Solid Earth. Effective extraction of higher mode dispersion information has been a research hotspot in the study of surface waves. In this study, we introduce a novel approach of refining Rayleigh wave higher modes by using seismoelectric signals,...

Dominant factors of the phosphorus regulatory network differ under various dietary phosphate loads in healthy individuals

Guoxin Ye, Jiaying Zhang, Zhaori Bi, Weichen Zhang, Minmin Zhang, Qian Zhang, Mengjing Wang & Jing Chen
The purpose of this study was to explore the contribution of each factor of the phosphorus metabolism network following phosphorus diet intervention via Granger causality analysis. In this study, a total of six healthy male volunteers were enrolled. All participants sequentially received regular, low-, and high-phosphorus diets. Consumption of each diet lasted for five days, with a 5-day washout period between different diets. Blood and urinary samples were collected on the fifth day of consumption...

Prognostic and immunological significance of metastasis associated lung adenocarcinoma transcript 1 among different kinds of cancers

Lili Guo, Xiuwen Zhang, Hongli Pan, Yang Li, Jing Wang, Lin Li, Yafang Dong, Xinxin Du, Jun Chen & Fengjie Guo
LncRNAs belong to the type of noncoding RNA transcripts, which exceed 200 nucleotides in size. MALAT1 as one of the earlier identified lncRNAs in cancer is investigated by more and more scientific researchers. Expression, clinical significance and function of MALAT1 in pan-cancer exist as big difference. To detect the expression and clinical significance of MALAT1 gene precisely and comprehensively among different kinds of cancers, some classical databases such as GEPIA, TIMER, KM Plotter, and PrognoScan...

Suppressed inflammation in obese children induced by a high-fiber diet is associated with the attenuation of gut microbial virulence factor genes

Hui Li, Guojun Wu, Liping Zhao & Menghui Zhang
In our previous study, a gut microbiota-targeted dietary intervention with a high-fiber diet improved the immune status of both genetically obese (Prader-Willi Syndrome, PWS) and simple obese (SO) children. However, PWS children had higher inflammation levels than SO children throughout the trial, the gut microbiota of the two cohorts was similar. As some virulence factors (VFs) produced by the gut microbiota play a role in triggering host inflammation, this study compared the characteristics and changes...

miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia

Zhongyan Zhao, Chanji Wu, Xiangying He, Eryi Zhao, Shijun Hu, Yeguang Han, Ting Wang, Yanquan Chen, Tao Liu & Shixiong Huang
Stroke is a main cause of disability and death worldwide, and ischemic stroke accounts for most stroke cases. Recently, microRNAs (miRNAs) have been verified to play critical roles in the development of stroke. Herein, we explored effects of miR-152-3p on vascular endothelial cell functions under hypoxia. Human umbilical vein endothelial cells (HUVECs) were treated with hypoxia to mimic cell injury in vitro. Reverse transcription quantitative polymerase chain reaction revealed that miR-152-3p exhibited high expression in...

Identification of the RP11-21C4.1/SVEP1 gene pair associated with FAT2 mutations as a potential biomarker in gastric cancer

Lingshan Zhou, Yuan Yang, Min Liu, Yuling Gan, Rong Liu, Man Ren, Ya Zheng, Yuping Wang & Yongning Zhou
Gastric cancer (GC) is one of the most common malignancies worldwide. Despite rapid advances in systemic therapy, GC remains the third leading cause of cancer-related deaths. We aimed to identify a novel prognostic signature associated with FAT2 mutations in GC. We analyzed the expression levels of FAT2-mutant and FAT2-wildtype GC samples obtained from The Cancer Genome Atlas (TCGA). The Kaplan–Meier survival curve showed that patients with FAT2 mutations showed better prognosis than those without the...

Identification of the RP11-21C4.1/SVEP1 gene pair associated with FAT2 mutations as a potential biomarker in gastric cancer

Lingshan Zhou, Yuan Yang, Min Liu, Yuling Gan, Rong Liu, Man Ren, Ya Zheng, Yuping Wang & Yongning Zhou
Gastric cancer (GC) is one of the most common malignancies worldwide. Despite rapid advances in systemic therapy, GC remains the third leading cause of cancer-related deaths. We aimed to identify a novel prognostic signature associated with FAT2 mutations in GC. We analyzed the expression levels of FAT2-mutant and FAT2-wildtype GC samples obtained from The Cancer Genome Atlas (TCGA). The Kaplan–Meier survival curve showed that patients with FAT2 mutations showed better prognosis than those without the...

A novel fusion circular RNA F-circBA1 derived from the BCR-ABL fusion gene displayed an oncogenic role in chronic myeloid leukemia cells

Yuan Tan, Zhenglan Huang, Xin Wang, Hongdan Dai, Guoyun Jiang & Wenli Feng
The BCR-ABL fusion gene plays a crucial role in the leukemogenesis of chronic myeloid leukemia (CML). The BCR-ABL oncoprotein encoded by this fusion gene has been extensively studied. However, research on whether BCR-ABL also affects circular RNAs (circRNAs) is limited. This study aimed to explore the new fusion circRNAs produced by BCR-ABL and their role in CML cells. In this study, we identified a novel fusion circRNA, named F-circBA1, originating from BCR-ABL in K562 and...

Paeoniflorin reduce luxS/AI-2 system-controlled biofilm formation and virulence in Streptococcus suis

Jinpeng Li, Qingying Fan, Manyu Jin, Chenlong Mao, Hui Zhang, Xiaoling Zhang, Liyun Sun, Daniel Grenier, Li Yi, Xiaogai Hou & Yang Wang
Streptococcus suis (S. suis), more specifically serotype 2, is a bacterial pathogen that threatens the lives of pigs and humans. Like many other pathogens, S. suis exhibits quorum sensing (QS) system-controlled virulence factors, such as biofilm formation that complicates treatment. Therefore, impairing the QS involving LuxS/AI-2 cycle in S. suis, may be a promising alternative strategy for overcoming S. suis infections. In this study, we investigated paeoniflorin (PF), a monoterpenoid glycoside compound extracted from peony,...

Inefficient integration during multiple facial processing in pre-morbid and early phases of psychosis

TianHong Zhang, YingYu Yang, LiHua Xu, XiaoChen Tang, YeGang Hu, Xin Xiong, YanYan Wei, HuiRu Cui, YingYing Tang, HaiChun Liu, Tao Chen, Zhi Liu, Li Hui, ChunBo Li, XiaoLi Guo & JiJun Wang
We used eye-tracking to evaluate multiple facial context processing and event-related potential (ERP) to evaluate multiple facial recognition in individuals at clinical high risk (CHR) for psychosis. In total, 173 subjects (83 CHRs and 90 healthy controls [HCs]) were included and their emotion perception performances were accessed. A total of 40 CHRs and 40 well-matched HCs completed an eye-tracking task where they viewed pictures depicting a person in the foreground, presented as context-free, context-compatible, and...

Whole-transcriptome analysis of rat cavernosum and identification of circRNA-miRNA-mRNA networks to investigate nerve injury erectile dysfunction pathogenesis

Jie Huang, Jianxiong Ma, Jie Wang, Ke Ma, Kang Zhou, Wenjie Huang, Fan Zhao, Bodong Lv & Qing Hu
There is growing evidence that circular RNAs (circRNAs) play a vital role in many kinds of diseases, including erectile dysfunction (ED). Nevertheless, the role of circRNAs in cavernous nerve-damaging ED (CNI-ED) is unknown. Here, we aimed to discover novel circRNAs, probed their potential role in the CNI-ED, and construct a ceRNA network of circRNAs. Twelve male Sprague Dawley rats were randomly divided into 2 groups by us: bilateral cavernous nerve crush (BCNC) and control groups....

Study of calcitriol anti-aging effects on human natural killer cells in vitro

Weiran Li, Xu Che, Xuemei Chen, Meiling Zhou, Xiaoping Luo & Tao Liu
Vitamin D is widely considered to have a regulatory effect on the immune system. Some clinical investigations have shown that the demand for vitamin D increases with age. Calcitriol is the biologically active form of vitamin D. However, its effect on human natural killer (NK) cells remains unclear. Therefore, in this study, we investigated the anti-aging and immunomodulatory effects of calcitriol on NK cells using a series of immunological methods to explore its important role...

Cell membrane-anchored anti-HIV single-chain antibodies and bifunctional inhibitors targeting the gp41 fusion protein: new strategies for HIV gene therapy

Yue Chen, Hongliang Jin, Xiaoran Tang, Li Li, Xiuzhu Geng, Yuanmei Zhu, Huihui Chong & Yuxian He
Emerging studies indicate that infusion of HIV-resistant cells could be an effective strategy to achieve a sterilizing or functional cure. We recently reported that glycosylphosphatidylinositol (GPI)-anchored nanobody or a fusion inhibitory peptide can render modified cells resistant to HIV-1 infection. In this study, we comprehensively characterized a panel of newly isolated HIV-1-neutralizing antibodies as GPI-anchored inhibitors. Fusion genes encoding the single-chain variable fragment (scFv) of 3BNC117, N6, PGT126, PGT128, 10E8, or 35O22 were constructed with...

Additional file 3 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 3: Table S2. Expression of wheat new miRNAs in crown tissues.

Additional file 4 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 4: Table S3. Information of wheat_miRNA2693 and wheat_miR5099.

Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018

Yanqun Wang, Xin Li, Wenkuan Liu, Mian Gan, Lu Zhang, Jin Wang, Zhaoyong Zhang, Airu Zhu, Fang Li, Jing Sun, Guoxian Zhang, Zhen Zhuang, Jiaying Luo, Dehui Chen, Shuyan Qiu, Li Zhang, Duo Xu, Chris Ka Pun Mok, Fuchun Zhang, Jingxian Zhao, Rong Zhou & Jincun Zhao
Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and...

Data from: Phylogenetic niche conservatism and variations in species diversity-climate relationships

Qinggang Wang, Yaoqi Li, Dongting Zou, Xiangyan Su, Hongyu Cai, Ao Luo, Ke Jiang, Xiaoling Zhang, Xiaoting Xu, Nawal Shrestha & Zhiheng Wang
Although contemporary climate has been identified as one of the major determinants of large-scale species diversity patterns, its effect on species diversity greatly varies among clades. Understanding the drivers of the variation in species diversity-climate relationships (DCRs) across clades, which is critical for developing general mechanisms underlying the effects of climate on species diversity patterns, remains a current challenge. Using newly compiled distribution data of 914 Rosaceae species in China and a dated genus-level phylogeny,...

Additional file 5 of Data integration and evolutionary analysis of long non-coding RNAs in 25 flowering plants

Shiye Sang, Wen Chen, Di Zhang, Xuan Zhang, Wenjing Yang & Changning Liu
Additional file 5. Download path of FASTA sequences for the lncRNAs from 25 plants.

Registration Year

  • 2021
    213

Resource Types

  • Dataset
    213

Affiliations

  • Peking University
    213
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    145
  • Fudan University
    130
  • Huazhong University of Science and Technology
    118
  • Capital Medical University
    115
  • Shanghai Jiao Tong University
    114
  • Sun Yat-sen University
    110
  • Zhejiang University
    110
  • Chinese Academy of Sciences
    110
  • Nanjing Medical University
    104