11 Works

Phosphorylation-Responsive Membrane Transport of Peptides

Dong-Sheng Guo, Werner Nau, Yu-Chen Pan, Andrea Barba-Bon, Andreas Hennig & Shu Peng
Until he gets you to the other side: Highly efficient counterion activation by amphiphilic calixarenes (gray cones) can mediate the transport of short peptide substrates for kinases across lipid memb...

Label-free continuous enzyme assays with macrocycle-fluorescent dye complexes

Andreas Hennig, Hüseyin Bakirci & Werner M Nau

Broad-Spectrum Tunable Photoluminescent Nanomaterials Constructed from a Modular Light-Harvesting Platform Based on Macrocyclic Amphiphiles

Zhe Xu, Shu Peng, Yu-Ying Wang, Ji-Kai Zhang, Alexandra I. Lazar & Dong-Sheng Guo
Broad-spectrum tunable photoluminescent nanomaterials are developed based on macrocyclic amphiphiles serving as a novel modular light-harvesting platform with discrete addressability of luminophores ...

Triple Emission fromp-Dimethylaminobenzonitrile-Cucurbit[8]uril Triggers the Elusive Excimer Emission

Mhejabeen Sayed, Frank Biedermann, Vanya D. Uzunova, Khaleel I. Assaf, Achikanath C. Bhasikuttan, Haridas Pal, Werner M. Nau & Jyotirmayee Mohanty
Abstract The intriguing dual-emission behavior of p- dimethylaminobenzonitrile (DMABN) and the identity of the associated excited states is, arguably, the most extensively investigated and also con...

Identification, classification, and signal amplification capabilities of high-turnover gas binding hosts in ultra-sensitive NMR

Andreas Hennig, Martin Kunth, Christopher Witte & Leif Schröder
Labile complexation of cucurbit[6]uril with xenon becomes detectable through saturation transfer with highly efficient gas turnover for ultra-sensitive NMR applications.

Inclusion of neutral guests by water-soluble macrocyclic hosts – a comparative thermodynamic investigation with cyclodextrins, calixarenes and cucurbiturils

Dong-Sheng Guo, Vanya D. Uzunova, Khaleel I. Assaf, Alexandra I. Lazar, Yu Liu & Werner M. Nau
(2016). Inclusion of neutral guests by water-soluble macrocyclic hosts – a comparative thermodynamic investigation with cyclodextrins, calixarenes and cucurbiturils. Supramolecular Chemistry: Vol. 28, Thirteenth International Conference on Calixarenes (Calix 2015), pp. 384-395.

Determining Protease Substrate Selectivity and Inhibition by Label-Free Supramolecular Tandem Enzyme Assays

Garima Ghale, Vijayakumar Ramalingam, Adam R. Urbach & Werner M. Nau
An analytical method has been developed for the continuous monitoring of protease activity on unlabeled peptides in real time by fluorescence spectroscopy. The assay is enabled by a reporter pair comprising the macrocycle cucurbit[7]uril (CB7) and the fluorescent dye acridine orange (AO). CB7 functions by selectively recognizing N-terminal phenylalanine residues as they are produced during the enzymatic cleavage of enkephalin-type peptides by the metalloendopeptidase thermolysin. The substrate peptides (e.g., Thr-Gly-Ala-Phe-Met-NH2) bind to CB7 with moderately...

Nanomolar Binding of Steroids to Cucurbit[n]urils: Selectivity and Applications

Alexandra I. Lazar, Frank Biedermann, Kamila R. Mustafina, Khaleel I. Assaf, Andreas Hennig & Werner M. Nau
Cucurbit[n]urils (CBn, n = 7, 8) serve as artificial receptors for steroids (21 tested), including the hormones testosterone and estradiol as well as steroidal drugs. Fluorescence displacement titrations and isothermal titration calorimetry (ITC) provided up to nanomolar binding affinities in aqueous solution for these hydrophobic target molecules, exceeding the values of known synthetic receptors. Remarkable binding selectivities, even for homologous steroid pairs, were investigated in detail by NMR, X-ray crystal diffraction, ITC, and quantum chemical...

Supramolecular Tandem Enzyme Assays for Multiparameter Sensor Arrays and Enantiomeric Excess Determination of Amino Acids

David M. Bailey, Andreas Hennig, Vanya D. Uzunova & Werner M. Nau
Abstract The coupling of an enzymatic transformation with dynamic host-guest exchange allows the unselective binding of macrocycles to be used for highly selective analyte sensing. The resulting su...

An Amphiphilic Sulfonatocalix[5]arene as an Activator for Membrane Transport of Lysine‐rich Peptides and Proteins

Werner Nau, Dong-Sheng Guo, Yu-Chen Pan, Andrea Barba-Bon, Han‐Wen Tian, Fei Ding & Andreas Hennig
An amphiphilic sulfonatocalix[5]arene (sCx5-6C) was rationally designed as an activator for membrane transport of lysine-rich peptides and proteins. sCx5-6C binds tightly with lysine and is able to e...

Registration Year

  • 2021
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Affiliations

  • Jacobs University
    11
  • Nankai University
    2
  • Institute of Nanotechnology
    2
  • Department of Chemistry and Earth Sciences
    2
  • State Key Laboratory of Polymer Physics and Chemistry
    2
  • European Remanufacturing Council
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  • Trinity University
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  • Karlsruhe Institute of Technology
    1