10 Works

Inhibition of Cdc42 activity extends lifespan and decreases circulating inflammatory cytokines in aged female C57BL/6 mice

Maria Carolina Florian, Hanna Leins, Michael Gobs, Yang Han, Gina Marka, Karin Soller, Angelika Vollmer, Vadim Sakk, Kalpana J. Nattamai, Ahmad Rayes, Xueheng Zhao, Kenneth Setchell, Medhanie Mulaw, Wolfgang Wagner, Yi Zheng & Hartmut Geiger
Cdc42 is a small RhoGTPase regulating multiple functions in eukaryotic cells. The activity of Cdc42 is significantly elevated in several tissues of aged mice, while the Cdc42 gain-of-activity mouse model presents with a pre-mature aging-like phenotype and with decreased lifespan. These data suggest a causal connection between elevated activity of Cdc42, aging and reduced lifespan. Here, we demonstrate that systemic treatment of aged (75-week old) female C57BL/6 mice with a Cdc42 activity specific inhibitor (CASIN)...

Contribution of males to brood care can compensate for their food consumption from a shared resource

Sandra Steiger, Eva M. Keppner & Manfred Ayasse
The sharing of the same food source among parents and offspring can be a driver of the evolution of family life and parental care. However, if all family members desire the same meal, competitive situations can arise, especially if resource depletion is likely. When food is shared for reproduction and the raising of offspring, parents have to decide whether they should invest in self-maintenance or in their offspring and it is not entirely clear how...

Long-term effects of antibiotic treatments on honeybee colony fitness – a modelling approach

Laura Bulson, Matthias Becher, Trevelyan McKinley & Lena Wilfert
1. Gut microbiome disequilibrium is increasingly implicated in host fitness reductions, including for the economically important and disease-challenged western honey bee, Apis mellifera. In lab experiments the antibiotic tetracycline, which is used to prevent American Foulbrood Disease in countries including the US, elevates honey bee mortality by disturbing the microbiome. It is unclear however, how elevated individual mortality affects colony level fitness. 2. We used an agent-based model (BEEHAVE) and empirical data to assess colony...

Adaptive changes in the genomes of wild rabbits after 16 years of viral epidemics

Stephen Pederson & Nina Schwensow
Since its introduction to control overabundant alien rabbits (Oryctolagus cuniculus), the highly virulent Rabbit Haemorrhagic Disease Virus (RHDV) has caused regular annual disease outbreaks in Australian rabbit populations. Although initially reducing rabbit abundance by 60%, continent-wide, experimental evidence has since indicated increased genetic resistance in wild rabbits that have experienced RHDV-driven selection. To identify genetic adaptations, which explain the increased resistance to this biocontrol virus, we investigated genome-wide SNP (single nucleotide polymorphism) allele frequency changes...

Offspring sex-ratios are stable across the life-course in Drosophila simulans

C. Ruth Archer, Matthew Carey, Tomohito Noda, Stefan Store & David Hosken
Within populations, adult sex ratios influence population growth and extinction risk, mating behaviors and parental care. Additionally, sex ratio adjustment can have pronounced effects on individual fitness. Accordingly, it is important that we understand how often, and why, offspring sex ratios deviate from parity. In Drosophila melanogaster, females appear to improve their fitness by producing fewer sons when paired with older males. However, facultative sex ratio adjustment in D. melanogaster is controversial, and our understanding...

Inferring quantity and qualities of superimposed reaction rates from single molecule survival time distributions

Matthias Reisser, Johannes Hettich, Timo Kuhn, Achim P. Popp, Andreas Große-Berkenbusch & J. Christof M. Gebhardt
Actions of molecular species, for example binding of transcription factors to chromatin, may comprise several superimposed reaction pathways. The number and the rate constants of such superimposed reactions can in principle be resolved by inverse Laplace transformation of the corresponding distribution of reaction lifetimes. However, current approaches to solve this transformation are challenged by photobleaching-prone fluorescence measurements of lifetime distributions. Here, we present a genuine rate identification method (GRID), which infers the quantity, rates and...

Topography strongly affects drought stress and xylem embolism resistance in woody plants from a karst forest in Southwest China

Qi-Wei Zhang, Shi-Dan Zhu, Steven Jansen & Kun-Fang Cao
1. Xylem resistance to drought-induced embolism is an important trait determining plant distribution. In the karst hills of Southwest China, with a relatively small variation in altitude, soil depth and water availability strongly decrease from the foot towards the top, and woody plant species display distinct spatial distribution. 2. For testing the hypothesis that embolism resistance of leaf and stem xylem reflects the spatial distribution across species along a topographical gradient of the karst hills,...

Data from: A prospective harmonized multicentre DTI study of cerebral white matter degeneration in ALS

Sanjay Kalra, Hans-Peter Müller, Abdullah Ishaque, Lorne Zinman, Lawrence Korngut, Angela Genge, Christian Beaulieu, Richard Frayne, Simon J. Graham & Jan Kassubek
Objective: To evaluate progressive white matter (WM) degeneration in ALS. Methods: Sixty-six patients with ALS and 43 healthy controls were enrolled in a prospective, longitudinal, multicentre study in the Canadian ALS Neuroimaging Consortium (CALSNIC). Participants underwent a harmonized neuroimaging protocol across 4 centres including diffusion tensor imaging (DTI) for assessment of WM integrity. Three visits were accompanied by clinical assessments of disability (ALSFRS-R) and upper motor neuron (UMN) function. Voxel-wise whole brain and quantitative tractwise...

Jumping the green wall: the use of PNA-DNA clamps to enhance microbiome sampling depth in wildlife microbiome research

Luis Viquez-R, Ramona Fleischer, Kerstin Wilhelm, Marco Tschapka & Simone Sommer
As microbiome research moves away from model organisms to wildlife, new challenges for microbiome high throughput sequencing arise caused by the variety of wildlife diets. High levels of contamination are commonly observed emanating from the host (mitochondria) or diet (chloroplast). Such high contamination levels affect the overall sequencing depth of wildlife samples thus decreasing statistical power and leading to poor performance in downstream analysis. We developed an amplification protocol utilizing PNA-DNA clamps to maximize the...

Forest habitat parameters influence abundance and diversity of cadaver-visiting dung beetles in Central Europe

Christian Von Hoermann, Sandra Weithmann, Markus Deißler, Manfred Ayasse & Sandra Steiger
Dung beetles (Scarabaeoidea) provide crucial ecosystem services and serve as model organisms for various behavioural, ecological and evolutionary studies. However, dung beetles have received little attention as consumers of large cadavers. In this study, we trapped copronecrophagous dung beetles on aboveground exposed piglet cadavers in 61 forest plots distributed over three geographically distinct regions in Germany, Central Europe. We examined the effects of land use intensity, forest stand, soil characteristics, vascular plant diversity and climatic...

Registration Year

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Affiliations

  • University of Ulm
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  • University of Bayreuth
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  • University of Exeter
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  • Institut d'Investigació Biomédica de Bellvitge
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  • University of Adelaide
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  • University of Alberta
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  • Cincinnati Children's Hospital Medical Center
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  • McGill University
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  • University of Freiburg
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  • Guangxi University
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