16 Works

Site impacts nutrient translocation efficiency in intra- and interspecies miscanthus hybrids on marginal lands

Elena Magenau, John Clifton-Brown, Danny Awty-Carroll, Chris Ashman, Andrea Ferrarini, Mislav Kontek, Enrico Martani, Kevin Roderick, Stefano Amaducci, Chris Davey, Vanja Jurišić, Jason Kam, Luisa Trindade, Iris Lewandowski & Andreas Kiesel
Miscanthus, a C4 perennial rhizomatous grass, is capable of growing in varied climates and soil types in Europe, including on marginal lands. It can produce high yields with low nutrient inputs when harvested after complete senescence. Senescence induction and rate depend on complex genetic, environmental, and management interactions. To explore these interactions, we analysed four miscanthus hybrids (two novel seed-based hybrids, GRC 3 (M. sinensis × sinensis) and GRC 14 (M. sacchariflorus × sinensis); GRC...

Additional file 4 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 4: Primers used for sgRNA off-target analysis.

Additional file 2 of Comprehensive transcriptional variability analysis reveals gene networks regulating seed oil content of Brassica napus

Zengdong Tan, Yan Peng, Yao Xiong, Feng Xiong, Yuting Zhang, Ning Guo, Zhuo Tu, Zhanxiang Zong, Xiaokun Wu, Jiang Ye, Chunjiao Xia, Tao Zhu, Yinmeng Liu, Hongxiang Lou, Dongxu Liu, Shaoping Lu, Xuan Yao, Kede Liu, Rod J. Snowdon, Agnieszka A. Golicz, Weibo Xie, Liang Guo & Hu Zhao
Additional file 2: Table S1. Genome-wide eQTLs identified at 20 DAF. Table S2. Genome-wide eQTLs identified at 40 DAF. Table S3. Genome-wide eGene-eQTLs identified at 20 DAF. Table S4. Genome-wide eGene-eQTLs identified at 40 DAF. Table S5. Results of GO enrichment analysis of specifically identified eGenes at 20 DAF. Table S6. Results of GO enrichment analysis of specifically identified eGenes at 40 DAF. Table S7. Summary of ATAC-Seq data. Table S8. List of homoeologous genes...

Sequestration of defenses against predators drives specialized host plant associations in preadapted milkweed bugs (Heteroptera: Lygaeinae)

Georg Petschenka, Rayko Halitschke, Tobias Züst, Anna Roth, Sabrina Stiehler, Linda Tenbusch, Christoph Hartwig, Juan Francisco Moreno Gámez, Robert Trusch, Jürgen Deckert, Kateřina Chalušová, Andreas Vilcinskas & Alice Exnerová
Host plant specialization across herbivorous insects varies dramatically, but while the molecular mechanisms of host-plant adaptations are increasingly known, we often lack a comprehensive understanding of the selective forces that favor specialization. The milkweed bugs (Heteroptera: Lygaeinae) are engaged in ancestrally specialized associations with plants of the Apocynaceae from which they commonly sequester cardiac glycosides for defense, facilitated by resistant Na+/K+-ATPases and adaptations for transport, storage and discharge of toxins. Here, we show that three...

Phenology of Lupinus polyphyllus from Central to Northern Europe

Kristin Ludewig, Yves P. Klinger, Tobias W. Donath, Lukas Bärmann, Carsten Eichberg, Jacob Gadegaad Thomsen, Eugen Görzen, Wiebke Hansen, Eliza M. Hasselquist, Thierry Helminger, Frida Kaiskog, Emma Karlsson, Torsten Kirchner, Carola Knudsen, Nikola Lenzewski, Sigrid Lindmo, Per Milberg, Daniel Pruchniewicz, Elisabeth Richter, Tobias M. Sandner, Judith M. Sarneel, Ralf Schmiede, Simone Schneider, Kathrin Schwarz, Åsa Tjäder … & Rolf Lutz Eckstein
Plant phenology, i. e. the timing of life cycle events, is related to individual fitness and species distribution ranges. Among the environmental factors, phenology is mostly driven by temperature and day length. Rapid adaptation of their phenology may also be important for the success of invasive plant species. Our main aim was to understand how the performance, timing, and temperature dependence of the phenology of the invasive legume Lupinus polyphyllus varies with latitude. L. polyphyllus...

Plant cover and plant-pollinator interactions in Central European grasslands (Poland/Czech Republic)

Demetra Rakosy, Elena Motivans, Valentin Ştefan, Arkadiusz Nowak, Sebastian Świerszcz, Reinart Feldmann, Elisabeth Kühn, Costanza Geppert, Neeraja Venkataraman, Anna Sobieraj-Betlińska, Anita Grossmann, Wiktoria Rojek, Katarzyna Pochrząst, Magdalena Cielniak, Anika Kirstin Gathof, Kevin Baumann & Tiffany Marie Knight
Complex socio-economic, political and demographic factors have driven the increased conversion of Europe’s semi-natural grasslands to intensive pastures. This trend is particularly strong in some of the most biodiverse regions of the continent, such as Central and Eastern Europe. Intensive grazing is known to decrease species diversity and alter the composition of plant and insect communities. Comparatively little is known, however, about how intensive grazing influences plant functional traits related to pollination and the structure...

Atropine-functionalised gold nanoparticles binding to muscarinic receptors after passage across the intestinal epithelium

Martin Diener
Gold nanoparticles have a high potential to be a treatment of diseases by their specific drug delivery properties and multivalent receptor stimulation. For the present project, spherical gold nanoparticles were synthesised and functionalised with the muscarinic receptor antagonist atropine (Au-MUDA-AT NPs). The diameter of the gold core could precisely be controlled by using different synthetic methods and reducing agents resulting in functionalised gold nanoparticles with diameters ranging from 8 to 16 nm. The ability to...

Marine Heterobranchia (Gastropoda, Mollusca) in Bunaken National Park, North Sulawesi, Indonesia—A Follow-Up Diversity Study

Jan-Hendrick Eisenbarth, Nani Undap, Adelfia Papu, Dorothee Schillo, Fontje Kaligis, Robert Bara, Till Schäberle, Gabriele M. König, Nathalie Yonow & Heike Wägele
Bunaken National Park has been surveyed for a fourth time in 14 years, in an attempt to establish the species composition of heterobranch sea slugs in a baseline study for monitoring programs and protection of this special park. These molluscs are potentially good indicators of the health of an ecosystem, as many are species-specific predators on a huge variety of marine benthic and sessile invertebrates from almost every taxonomic group. Additionally, they are known to...

Second survey of heterobranch sea slugs (Mollusca, Gastropoda, Heterobranchia) from Bunaken National Park, North Sulawesi, Indonesia - how much do we know after 12 years

Fontje Kaligis, Jan-Hendrik Eisenbarth, Dorothee Schillo, Till Schäberle, Nils Böhringer, Robert Bara, Gabriele M. König & Heike Wägele
Bunaken National Park (BNP) is one of the most famous marine national parks in Indonesia with an extraordinary diversity in marine life forms. However, this diversity is threatened by an increasing population on the islands, ongoing destructive fishing techniques and lately by an increase in tourism. Protecting and managing the future use of BNP resources will require the assessment of both, local marine biodiversity through monitoring efforts and the identification and subsequent reduction of any...

Nitrogen availability and plant functional composition modify biodiversity-multifunctionality relationships

Eric Allan, Noémie Pichon, Seraina Cappelli, Santiago Soliveres, Tosca Mannall, Thu Zar Nwe, Norbert Hölzel, Valentin Klaus, Till Kleinebecker & Hugo Vincent
The ability of an ecosystem to deliver multiple functions at high levels (multifunctionality) typically increases with biodiversity but there is substantial variation in the strength and direction of biodiversity effects, suggesting context-dependency. A better understanding of the drivers of this context dependency is essential to predict effects of global change on ecosystems. To determine how different factors modulate the effect of diversity on multifunctionality, we established a large grassland experiment with 216 communities, crossing a...

Additional file 3 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 3: sgRNA off-target analysis. Trp53, Rb1 and Rbl2 sgRNAs were analyzed for potential off-targets using the CRISPRoff Tool version 1.2. Shown are the Top10 hits with an intragenic location and “NGG” PAM. The Top10 hits for each of the Trp53, Rb1 and Rbl2 sgRNAs were PCR amplified using genomic tumor DNA from 3 AV-PR.Cre and 3 AV-PRL.Cre infected mice. PCR amplicons were analyzed for mutations by Sanger sequencing. Results are shown color-coded. Identified...

Additional file 3 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 3: sgRNA off-target analysis. Trp53, Rb1 and Rbl2 sgRNAs were analyzed for potential off-targets using the CRISPRoff Tool version 1.2. Shown are the Top10 hits with an intragenic location and “NGG” PAM. The Top10 hits for each of the Trp53, Rb1 and Rbl2 sgRNAs were PCR amplified using genomic tumor DNA from 3 AV-PR.Cre and 3 AV-PRL.Cre infected mice. PCR amplicons were analyzed for mutations by Sanger sequencing. Results are shown color-coded. Identified...

Additional file 4 of Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase

Nastasja Merle, Sabrina Elmshäuser, Florian Strassheimer, Michael Wanzel, Alexander M. König, Julianne Funk, Michelle Neumann, Katharina Kochhan, Frederik Helmprobst, Axel Pagenstecher, Andrea Nist, Marco Mernberger, André Schneider, Thomas Braun, Tilman Borggrefe, Rajkumar Savai, Oleg Timofeev & Thorsten Stiewe
Additional file 4: Primers used for sgRNA off-target analysis.

Data from: Links across ecological scales: Plant biomass responses to elevated CO2

Julia Maschler, Lalasia Bialic-Murphy, Joe Wan, Louise C. Andresen, Constantin M. Zohner, Peter B. Reich, Andreas Lüscher, Manuel K. Schneider, Christoph Müller, Gerald Moser, Jeffrey S. Dukes, Inger Kappel Schmidt, Mark C. Bilton, Kai Zhu & Thomas W. Crowther
Despite the wide agreement that increased plant biomass accumulation under elevated CO2 concentrations (e[CO2]) might play a key role in climate change, the effect of e[CO2] on plant biomass levels remains a major uncertainty in climate models. In the review associated with this dataset, we discuss the evidence for increased biomass levels under e[CO2] across multiple levels of ecological organization, scaling from physiological responses to changes in population-, community-, ecosystem-, and global-scale dynamics. We find...

Additional file 2 of Comprehensive transcriptional variability analysis reveals gene networks regulating seed oil content of Brassica napus

Zengdong Tan, Yan Peng, Yao Xiong, Feng Xiong, Yuting Zhang, Ning Guo, Zhuo Tu, Zhanxiang Zong, Xiaokun Wu, Jiang Ye, Chunjiao Xia, Tao Zhu, Yinmeng Liu, Hongxiang Lou, Dongxu Liu, Shaoping Lu, Xuan Yao, Kede Liu, Rod J. Snowdon, Agnieszka A. Golicz, Weibo Xie, Liang Guo & Hu Zhao
Additional file 2: Table S1. Genome-wide eQTLs identified at 20 DAF. Table S2. Genome-wide eQTLs identified at 40 DAF. Table S3. Genome-wide eGene-eQTLs identified at 20 DAF. Table S4. Genome-wide eGene-eQTLs identified at 40 DAF. Table S5. Results of GO enrichment analysis of specifically identified eGenes at 20 DAF. Table S6. Results of GO enrichment analysis of specifically identified eGenes at 40 DAF. Table S7. Summary of ATAC-Seq data. Table S8. List of homoeologous genes...

Target enrichment of long open reading frames and ultraconserved elements to link microevolution and macroevolution in non-model organisms

Claudia Ortiz-Sepulveda, Mathieu Genete, Christelle Blassiau, Cécile Godé, Christian Albrecht, Xavier Vekemans & Bert Van Bocxlaer
Despite the increasing accessibility of high-throughput sequencing, obtaining high-quality genomic data on non-model organisms without proximate well-assembled and annotated genomes remains challenging. Here we describe a workflow that takes advantage of distant genomic resources and ingroup transcriptomes to select and jointly enrich long open reading frames (ORFs) and ultraconserved elements (UCEs) from genomic samples for integrative studies of microevolutionary and macroevolutionary dynamics. This workflow is applied to samples of the African unionid bivalve tribe Coelaturini...

Registration Year

  • 2022
    16

Resource Types

  • Dataset
    16

Affiliations

  • University of Giessen
    16
  • University of Würzburg
    4
  • Max Planck Institute for Heart and Lung Research
    4
  • Philipps University of Marburg
    4
  • University of Bern
    3
  • North West Agriculture and Forestry University
    2
  • Agricultural Genomics Institute at Shenzhen
    2
  • Shanghai University
    2
  • West China Hospital of Sichuan University
    2
  • Zhejiang University
    2