Alcohol consumption affects many organs and tissues, including skeletal muscle. However, the molecular mechanism of ethanol action on skeletal muscle remains unclear. Here, using molecular dynamics simulations and single channel recordings, we show that ethanol interacts with a negatively charged amino acid within an extracellular region of the neuromuscular nicotinic acetylcholine receptor (nAChR), thereby altering its global conformation and reducing the single channel current amplitude. Charge reversal of the negatively charged amino acid abolishes the...
Data from: Frequency and characteristics of MODY 1 (HNF4A mutation) and MODY 5 (HNF1B mutation) - Analysis from the DPV databaseKatharina Warncke, Sebastian Kummer, Klemens Raile, Jürgen Grulich-Henn, Joachim Woelfle, Elisabeth Steichen, Nicole Prinz & Reinhard W. Holl
Objective. To characterize initial presentation and clinical course of patients with hepatocyte nuclear factor (HNF) 4A- and HNF1B-MODY in a multinational registry. Design, setting and participants. Within the Diabetes Patienten Verlaufsdokumentation (DPV) registry, 44 patients with HNF4A- and 35 patients with HNF1B-MODY were characterized and compared with patients < 20years old with type 1 diabetes (T1D)/type 2 diabetes (T2D). Main outcome measure. Clinical and laboratory parameters, therapy, metabolic control, and extrapancreatic symptoms in patients with...
Data from: Out of the Mediterranean? post-glacial colonisation pathways varied among cold-water coral speciesJoana Boavida, Ronan Becheler, Marvin Choquet, Norbert Frank, Marco Taviani, Jean-Francois Bourillet, Anne-Leila Meistertzheim, Anthony Grehan, Alessandra Savini & Sophie Arnaud-Haond
Aim. To infer cold-water corals' post-glacial phylogeography and assess the role of Mediterranean Sea glacial refugia as origins for the recolonisation of the northeastern Atlantic Ocean. Location. Northeastern Atlantic Ocean and Mediterranean Sea. Taxon. Lophelia pertusa, Madrepora oculata. Methods. We sampled cold-water corals using remotely operated vehicles and one sediment core for coral and sediment dating. We characterized spatial genetic patterns (microsatellites and a nuclear gene fragment) using networks, clustering and measures of genetic differentiation....
Genetic admixture despite ecological segregation in a North African sparrow hybrid zone (Aves, Passeriformes, Passer domesticus x Passer hispaniolensis)Martin Päckert, Abdelkrim Belkacem, Hannes Wolfgramm, Oliver Gast, David Canal, Gabriele Giacalone, Mario Lo Valvo, Melita Vamberger, Michael Wink, Jochen Martens & Heiko Stuckas
Under different environmental conditions hybridization between the same species might result in different patterns of genetic admixture. Particularly, species pairs with large distribution ranges and long evolutionary history may have experienced several independent hybridization events over time in different zones of overlap. In birds, the diverse hybrid populations of the house sparrow (Passer domesticus) and the Spanish sparrow (P. hispaniolensis) provide a striking example. Throughout their range of sympatry, these two species do not regularly...
Data from: Single-molecule imaging and quantification of the immune-variant adhesin VAR2CSA on knobs of Plasmodium falciparum-infected erythrocytesCecilia P. Sanchez, Christos Karathanasis, Rodrigo Sanchez, Marek Cyrklaff, Julia Jäger, Bernd Buchholz, Ulrich S. Schwarz, Mike Heilemann & Michael Lanzer
PfEMP1 (erythrocyte membrane protein 1) adhesins play a pivotal role in the pathophysiology of falciparum malaria, by mediating sequestration of Plasmodium falciparum-infected erythrocytes in the microvasculature. PfEMP1 variants are expressed by var genes and are presented on membrane elevations, termed knobs. However, the organization of PfEMP1 on knobs is largely unclear. Here, we use super-resolution microscopy and genetically altered parasites expressing a modified var2csa gene in which the coding sequence of the photoactivatable mEOS2 was...
Universitaire Ziane Achour de Djelfa1
Estación Biológica de Doñana1
Senckenberg Nature Research Society1
University Hospital Heidelberg1
University of Milano-Bicocca1
Johannes Gutenberg University of Mainz1
Max Planck Institute for Biological Cybernetics1
French National Centre for Scientific Research1
Technical University Munich1