9 Works

Data from: Collagen secretion screening in Drosophila supports a common secretory machinery and multiple Rab requirements

Hongmei Ke, Zhi Feng, Min Liu, Tianhui Sun, Jianli Dai, Mengqi Ma, Lu-Ping Liu, Jian-Quan Ni & José Carlos Pastor-Pareja
Collagens are large secreted trimeric proteins making up most of the animal extracellular matrix. Secretion of collagen has been a focus of interest for cell biologists in recent years because collagen trimers are too large and rigid to fit into the COPII vesicles mediating transport from the endoplasmic reticulum (ER) to the Golgi. Collagen-specific mechanisms to create enlarged ER-to-Golgi transport carriers have been postulated, including cargo loading by conserved ER exit site (ERES) protein Tango1....

Data from: Biofilm inhibition and pathogenicity attenuation in bacteria by Proteus mirabilis

Shichen Yu, Xiaoshan Zhu, Jin Zhou & Zhonghua Cai
Biofilms play an important role in the antibiotic resistance of encased bacteria, and their formation was regulated by quorum sensing (QS). Inhibiting the QS system may therefore degrade the integrity of a biofilm and expose the bacterial pathogens within it to the deleterious effects of molecules such as antibiotics. Moreover, the use of QS inhibitors (QSIs) may provide a novel approach for treating bacterial infections of aquacultures. In the present study, the bacterium Proteus mirabilis...

Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro

Bo Yang, Qinghong Xue, Xuefeng Qi, Xueping Wang, Peilong Jia, Shuying Chen, Ting Wang, Tianxia Xue & Jingyu Wang
Peste des petits ruminants (PPR) is an acute and highly contagious disease in small ruminants that causes significant economic losses in developing countries. An increasing number of studies have demonstrated that both autophagy and apoptosis are important cellular mechanisms for maintaining homeostasis, and they participate in the host response to pathogens. However, the crosstalk between apoptosis and autophagy in host cells during PPRV infection has not been clarified. In this study, autophagy was induced upon...

Data from: Rapid detection of cocaine using aptamer-based biosensor on an evanescent wave fibre platform

Yong Qiu, Yunfei Tang, Bing Li & Miao He
The rapid detection of cocaine has received considerable attention because of the instantaneous and adverse effects of cocaine overdose on human health. Aptamer-based biosensors for cocaine detection have been well established for research and application. However, reducing the analytic duration without deteriorating the sensitivity still remains as a challenge. Here, we proposed an aptamer-based evanescent wave fiber (EWF) biosensor to rapidly detect cocaine in a wide working range. At first, the aptamers were conjugated to...

Data from: Association between aspirin dose and subarachnoid hemorrhage from saccular aneurysms: a case-control study

Anil Can, Robert F. Rudy, Victor M. Castro, Sheng Yu, Dmitriy Dligach, Sean Finan, Vivian Gainer, Nancy A. Shadick, Guergana Savova, Shawn Murphy, Tianxi Cai, Scott T. Weiss & Rose Du
Objective: We aimed to determine the association between ruptured saccular aneurysms and aspirin use/aspirin dose. Methods: 4,701 patients who were diagnosed at the Massachusetts General Hospital and Brigham and Women’s Hospital between 1990 and 2016 with 6,411 unruptured and ruptured saccular intracranial aneurysms were evaluated. Univariable and multivariable logistic regression analyses were performed to determine the association between aSAH and aspirin use, including aspirin dose. Inverse probability weighting using propensity scores was used to adjust...

Data from: Fluorescence and photophysical properties of xylene isomers in water: with experimental and theoretical approaches

Muhammad Farooq Saleem Khan, Jing Wu, Bo Liu, Cheng Cheng, Mona Akbar, Yidi Chai & Aisha Memon
A thorough analysis of the photophysical properties involved in electronic transitions in excitation–emission spectra of xylene isomers has been carried out using the time-dependent density functional theory (PBEPBE/6-31 + G(d,p)) method. For the first time a structural and spectroscopic investigation to distinguish isomers of xylene, a widespread priority pollutant, was conducted experimentally and theoretically. The fluorescence properties of xylene isomers (sole and mixture (binary and ternary)) in water were studied. The fluorescence peak intensities of...

Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro

Bo Yang, Qinghong Xue, Xuefeng Qi, Xueping Wang, Peilong Jia, Shuying Chen, Ting Wang, Tianxia Xue & Jingyu Wang
Peste des petits ruminants (PPR) is an acute and highly contagious disease in small ruminants that causes significant economic losses in developing countries. An increasing number of studies have demonstrated that both autophagy and apoptosis are important cellular mechanisms for maintaining homeostasis, and they participate in the host response to pathogens. However, the crosstalk between apoptosis and autophagy in host cells during PPRV infection has not been clarified. In this study, autophagy was induced upon...

Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin

Wenlong Zhang, Haili Wang, Bing Wang, Yue Zhang, Yunhao Hu, Bo Ma & Junwei Wang
Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen. Pyolysin (PLO) importantly contributes to the pathogenicity of T. pyogenes. However, the relationship between the structure and function and the virulence of PLO is not well documented. In the current study, recombinant PLO (rPLO) and three rPLO mutants were prepared. rPLO D238R, a mutant with the 238th aspartic acid replaced with an arginine, showed impairment in oligomerization activity on cholesterol-containing liposome and pore-forming activity on sheep...

Replacing the 238th aspartic acid with an arginine impaired the oligomerization activity and inflammation-inducing property of pyolysin

Wenlong Zhang, Haili Wang, Bing Wang, Yue Zhang, Yunhao Hu, Bo Ma & Junwei Wang
Trueperella pyogenes (T. pyogenes) is an important opportunistic pathogen. Pyolysin (PLO) importantly contributes to the pathogenicity of T. pyogenes. However, the relationship between the structure and function and the virulence of PLO is not well documented. In the current study, recombinant PLO (rPLO) and three rPLO mutants were prepared. rPLO D238R, a mutant with the 238th aspartic acid replaced with an arginine, showed impairment in oligomerization activity on cholesterol-containing liposome and pore-forming activity on sheep...

Registration Year

  • 2018
    9

Resource Types

  • Dataset
    9

Affiliations

  • Tsinghua University
    9
  • Zhejiang University
    4
  • Huazhong University of Science and Technology
    4
  • Second Affiliated Hospital of Chongqing Medical University
    4
  • Second Affiliated Hospital of Xi'an Jiaotong University
    4
  • Nanjing Medical University
    3
  • Children's Hospital of Chongqing Medical University
    3
  • Nanjing Agricultural University
    3
  • Shanghai University
    2
  • Jilin University
    2