4 Works

Data from: The sickle cell trait affects contact dynamics and endothelial cell activation in Plasmodium falciparum-infected erythrocytes

Christine Lansche, Anil K. Dasanna, Katharina Quadt, Benjamin Fröhlich, Dimitris Missirlis, Marilou Tetard, Benoit Gamain, Bernd Buchholz, Cecilia P. Sanchez, Motomu Tanaka, Ulrich S. Schwarz & Michael Lanzer
Sickle cell trait, a common hereditary blood disorder, protects carriers from severe disease in infections with the human malaria parasite Plasmodium falciparum. Protection is associated with a reduced capacity of parasitized erythrocytes to cytoadhere to the microvascular endothelium and cause vaso-occlusive events. However, the underpinning cellular and biomechanical processes are only partly understood and the impact on endothelial cell activation is unclear. Here, we show, by combining quantitative flow chamber experiments with multiscale computer simulations...

Data from: Reduced PICD in monocytes mounts altered neonate immune response to Candida albicans

Mathias Hornef, Stephan Dreschers, Christopher Platen, Joachim Morschhäuser, Peter Saupp, Andrea Prehn, Thorsten Orlikowsky & Thorsten W. Orlikowsky
Background: Invasive fungal infections with Candida albicans (C. albicans) occur frequently in extremely low birthweight (ELBW) infants and are associated with poor outcome. Phagocytosis of C.albicans initializes apoptosis in monocytes (phagocytosis induced cell death, PICD). PICD is reduced in neonatal cord blood monocytes (CBMO). Hypothesis: Phagocytosis of C. albicans causes PICD which differs between neonatal monocytes (CBMO) and adult peripheral blood monocytes (PBMO) due to lower stimulation of TLR-mediated immune responses. Methods: The ability to...

Data from: Reduced internalization of TNF-ɑ/TNFR1 down-regulates caspase dependent phagocytosis induced cell death (PICD) in neonatal monocytes

Stephan Dreschers, Christian Gille, Martin Haas, Florence Seubert, Christopher Platen & Thorsten W. Orlikowsky
Phagocytosis-induced cell death (PICD) is diminished in cord blood monocytes (CBMO) as compared to cells from adults (PBMO) due to differences in the CD95-pathway. This may support a prolonged pro-inflammatory response with sequels of sustained inflammation as seen in neonatal sepsis. Here we hypothesized that TNF-α mediated induction of apoptosis is impaired in CBMO due to differences in the TNFR1-dependent internalization. Monocytes were infected with Escherichia coli-GFP (E. coli-GFP). Monocyte phenotype, phagocytic activity, induction of...

Data from: Single-molecule imaging and quantification of the immune-variant adhesin VAR2CSA on knobs of Plasmodium falciparum-infected erythrocytes

Cecilia P. Sanchez, Christos Karathanasis, Rodrigo Sanchez, Marek Cyrklaff, Julia Jäger, Bernd Buchholz, Ulrich S. Schwarz, Mike Heilemann & Michael Lanzer
PfEMP1 (erythrocyte membrane protein 1) adhesins play a pivotal role in the pathophysiology of falciparum malaria, by mediating sequestration of Plasmodium falciparum-infected erythrocytes in the microvasculature. PfEMP1 variants are expressed by var genes and are presented on membrane elevations, termed knobs. However, the organization of PfEMP1 on knobs is largely unclear. Here, we use super-resolution microscopy and genetically altered parasites expressing a modified var2csa gene in which the coding sequence of the photoactivatable mEOS2 was...

Registration Year

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  • Dataset
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Affiliations

  • University Children's Hospital Tübingen
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  • University Hospital Heidelberg
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  • Heidelberg University
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  • Max Planck Institute for Medical Research
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  • Biologie Intégrée du Globule Rouge
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  • Goethe University Frankfurt
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