70 Works

A manager’s guide to using eDNA metabarcoding in marine ecosystems

Zachary Gold, Adam R. Wall, Teia M. Schweizer, N. Dean Pentcheff, Emily E. Curd, Paul H. Barber, Rachel S. Meyer, Robert Wayne, Kevin Stolzenbach, Kat Prickett, Justin Luedy & Regina Wetzer
Environmental DNA (eDNA) metabarcoding is a powerful tool that can enhance marine ecosystem/biodiversity monitoring programs. Here we outline five important steps managers and researchers should consider when developing eDNA monitoring program: 1) select genes and primers to target focal taxa; 2) assemble or develop comprehensive barcode reference databases; 3) apply rigorous site occupancy based decontamination pipelines; 4) conduct pilot studies to define spatial and temporal variance of eDNA; and 5) archive samples, extracts, and raw...

Population genetics reveals bidirectional fish movement across the Continental Divide via an interbasin water transfer

Audrey Harris, Sara Oyler-McCance, Jennifer Fike, Matthew Fairchild, Christopher Kennedy, Harry Crockett, Dana Winkelman & Yoichiro Kanno
Interbasin water transfers are becoming an increasingly common tool to satisfy municipal and agricultural water demand, but their impacts on movement and gene flow of aquatic organisms are poorly understood. The Grand Ditch is an interbasin water transfer that diverts water from tributaries of the upper Colorado River on the west side of the Continental Divide to the upper Cache la Poudre River on the east side of the Continental Divide. We used single nucleotide...

Shifting hotspots: Climate change projected to drive contractions and expansions of invasive plant abundance ranges

Annette Evans, Evelyn M. Beaury, Peder S. Engelstad, Nathan B. Teich & Bethany A. Bradley
This file contains maps of current and future abundance suitable habitat for 144 invasive plant species in the United States. Each tiff file represents the current or future range prediction maps of habitat suitable for supporting abundant populations (greater than or equal to 5% cover) of 144 invasive plant taxa, projected across the lower 48 States of the United States. Each tiff file is named with the USDA species code (SpCode) (see 'Species_information.xlsx file for...

Filtered phytoplankton movement data

Hannah Meier, Isaac Schuman, Tamara Layden, Anna Ritz, Colin Kremer & Samuel Fey
A prerequisite for the survival and reproduction of organisms is to successfully navigate thermal environmental conditions that unfold over time and space. While effective movement behaviour has been highlighted as a key mechanism by which organisms and populations may persist amidst the backdrop of directional environmental warming, it remains unclear how behavioural plasticity may mediate such effects, particularly across timescales that span multiple generations. Here, we examine the capacity for transgenerational plasticity to alter the...

Supplementary data for: Comparison of optical flow derivation techniques for retrieving tropospheric winds from satellite image sequences

Jason Apke
This study introduces a validation technique for quantitative comparison of algorithms which retrieve winds from passive detection of cloud- and water vapor-drift motions, also known as Atmospheric Motion Vectors (AMVs). The technique leverages airborne wind-profiling lidar data collected in tandem with 1-min refresh rate geostationary satellite imagery. AMVs derived with different approaches are used with accompanying numerical weather prediction model data to estimate the full profiles of lidar-sampled winds which enables ranking of feature tracking,...

Capture history data for: Sub-seasonal correlation between growth and survival in three sympatric aquatic ectotherms

Yoichiro Kanno, Seoghyun Kim & Kasey Pregler
Animals experience seasonally changing conditions in temperate regions, thus population vital rates change seasonally. However, knowledge is lacking on patterns of seasonal correlation between growth and survival in sympatric ectotherms, and this knowledge gap limits our understanding of environmental change impacts on animal populations and communities. Here, we investigated sub-seasonal (2-month intervals) correlation between growth and survival in three stream fishes (bluehead chub Nocomis leptocephalus, creek chub Semotilus atromaculatus, and mottled sculpin Cottus bairdii) in...

Data for: Single-blind determination of methane detection limits and quantification accuracy using aircraft-based LiDAR

Clay Bell, Jeff Rutherford, Adam Brandt, Evan Sherwin, Timothy Vaughn & Daniel Zimmerle
Methane detection limits, emission rate quantification accuracy, and potential cross-species interference are assessed for Bridger Photonics’ Gas Mapping LiDAR (GML) system utilizing data collected during laboratory testing and single-blind controlled release testing. Laboratory testing identified no significant interference in the path-integrated methane measurement from the gas species tested (ethylene, ethane, propane, n-butane, i-butane, and carbon dioxide). The controlled release study, comprised of 650 individual measurement passes, represents the largest dataset collected to date to characterize...

Plant species with higher chemical defenses enhance herbivore cellular immunity with differential effectiveness against two parasitoid species

Enakshi Ghosh, Paul Ode & Ryan Paul
Insect herbivores simultaneously experience bottom-up effects of plant defensive chemistry and the top-down effects of natural enemies. At the intersection of these effects are herbivore immune systems, herbivore traits that have largely been overlooked in studies of plant-insect interactions. Most previous studies have demonstrated compromised immunity of herbivores that feed on plants with higher defensive chemistry. Many studies have used embedded microfilaments or silica beads as proxies for parasitoid eggs. Yet, parasitoids may evade or...

Root Production and Morphological Traits During and After Single and Repeated Extreme Droughts in a Mesic Grassland

Ingrid Slette, David Hoover, Melinda Smith & Alan Knapp
Global climate change is expected to cause more frequent extreme droughts in many parts of the world. Despite the crucial role of roots in water acquisition and plant survival, our understanding of ecosystem vulnerability to drought is primarily based on aboveground impacts. As return intervals between droughts decrease, root responses to one drought might alter responses to subsequent droughts, but this remains unresolved. We conducted a 7-year experiment that imposed extreme drought (growing season precipitation...

Genome size drives morphological evolution in organ-specific ways

Michael Itgen
Morphogenesis is an emergent property of biochemical and cellular interactions during development. Genome size and the correlated trait of cell size can influence these interactions through effects on developmental rate and tissue geometry, ultimately driving the evolution of morphology. We tested whether variation in genome and body size is related to morphological variation in the heart and liver using nine species of the salamander genus Plethodon (genome sizes 29–67 gigabases). Our results show that overall...

Leaf gas exchange in Ipomopsis aggregata under manipulated snowmelt timing and summer precipitation

Jocelyn Navarro, John Powers, Ayaka Paul & Diane Campbell
Vegetative traits of plants can respond directly to changes in the environment, such as those occurring under climate change. That phenotypic plasticity could be adaptive, maladaptive, or neutral. We manipulated the timing of spring snowmelt and amount of summer precipitation in factorial combination and examined the responses of photosynthetic rate, stomatal conductance, and intrinsic water-use efficiency (iWUE) in the subalpine herb Ipomopsis aggregata. The experiment was repeated in three years differing in natural timing of...

Water availability dictates how plant traits predict demographic rates

Alice Stears, Peter Adler, Dana Blumenthal, Julie Kray, Kevin Mueller, Troy Ocheltree, Kevin Wilcox & Daniel Laughlin
A major goal in ecology is to make generalizable predictions of organism responses to environmental variation based on their traits. However, straightforward relationships between traits and fitness are rare and likely vary with environmental context. Characterizing how traits mediate demographic responses to the environment may enhance predictions of organism responses to global change. We synthesized 15 years of demographic data and species-level traits in a shortgrass steppe to determine whether the effects of leaf and...

Data from: Land-based climate solutions for the United States

G. Philip Robertson, Stephen Hamilton, Keith Paustian & Pete Smith
Meeting end-of-century global warming targets requires aggressive action on multiple fronts. Recent reports note the futility of addressing mitigation goals without fully engaging the agricultural sector, yet no available assessments combine both nature-based solutions (reforestation, grassland and wetland protection, and agricultural practice change) and cellulosic bioenergy for a single geographic region. Collectively, these solutions might offer a suite of climate, biodiversity, and other benefits greater than either alone. Nature-based solutions are largely constrained by the...

Data from: Rocky Mountain forests are poised to recover following bark beetle outbreaks, but with altered composition

Kyle Rodman, Robert Andrus, Amanda Carlson, Trevor Carter, Teresa Chapman, Jonathan Coop, Paula Fornwalt, Nathan Gill, Brian Harvey, Ashley Hoffman, Katharine Kelsey, Dominik Kulakowski, Daniel Laughlin, Jenna Morris, José Negrón, Katherine Nigro, Gregory Pappas, Miranda Redmond, Charles Rhoades, Monique Rocca, Zoe Schapira, Jason Sibold, Camille Stevens-Rumann, Thomas Veblen, Jianmin Wang … & Sarah Hart
Amplified by warming temperatures and drought, recent outbreaks of native bark beetles (Curculionidae: Scolytinae) have caused extensive tree mortality throughout Europe and North America. Despite their ubiquitous nature and important effects on ecosystems, forest recovery following such disturbances is poorly understood, particularly across regions with varying abiotic conditions and outbreak effects. To better understand post-outbreak recovery across a topographically complex region, we synthesized data from 16 field studies spanning subalpine forests in the Southern Rocky...

How community forest management performs when REDD+ payments fail

Amy Collins, Mark Grote, Tim Caro, Aniruddha Ghosh, James Thorne, Jonathan Salerno & Monique Borgerhoff Mulder
The reduced emissions in deforestation and degradation (REDD+) initiative uses payments for ecosystem services as incentives for developing countries to manage and protect their forests. REDD+ initiatives also prioritize social (and environmental) co-benefits aimed at improving the livelihoods of communities that are dependent on forests. Despite the incorporation of co-benefits into REDD+ goals, carbon sequestration remains the primary metric for which countries can receive payments from REDD+, but after more than 10 years of REDD+,...

Large-scale reforestation can increase water yield and reduce drought risk for water-insecure regions in the Asia-Pacific

Hoong Chen Teo, Srivatsan Raghavan, Xiaogang He, Zhenzhong Zeng, Yanyan Cheng, Xiangzhong Luo, Alex Lechner, Matthew Ashfold, Aakash Lamba, Rachakonda Sreekar, Qiming Zheng, Anping Chen & Lian Pin Koh
Large-scale reforestation can potentially bring both benefits and risks to the water cycle, which needs to be better quantified under future climates to inform reforestation decisions. We identified 477 water-insecure basins worldwide accounting for 44.6% (380.2 Mha) of the global reforestation potential. As many of these basins are in the Asia-Pacific, we used regional coupled land-climate modelling for the period 2041–2070 to reveal that reforestation increases evapotranspiration and precipitation for most water-insecure regions over the...

Data from: Trading water for carbon in the future: effects of elevated CO2 and warming on leaf hydraulic traits in a semiarid grassland

Kevin Mueller, Dana Blumenthal, Troy Ocheltree & Julie Kray
The effects of climate change on plants and ecosystems are mediated by plant hydraulic traits, including interspecific and intraspecific variability of trait phenotypes. Yet, integrative and realistic studies of hydraulic traits and climate change are rare. In a semiarid grassland, we assessed the response of several plant hydraulic traits to elevated CO2 (+200 ppm) and warming (+1.5 to 3℃; day to night). For leaves of five dominant species (three graminoids, two forbs), and in replicated...

Conservation genetics of an island-endemic lizard: low Ne and the critical role of intermediate temperatures for genetic connectivity

Daryl Trumbo, W. Chris Funk, Gregory Pauly & Jeanne Robertson
Island populations are at higher risk of extinction than mainland populations. Therefore, understanding the factors that facilitate connectivity is particularly pressing for the conservation of island taxa. Sceloporus occidentalis becki, the Island Fence Lizard, is an endemic taxon restricted to the Northern Channel Islands, part of a nearshore archipelago in Southern California, USA. Since the Last Glacial Maximum, fence lizard habitat on the Northern Channel Islands has decreased with rising sea levels and increasing temperatures...

Dataset for Intrinsic Century Scale Variability in Tropical Pacific Sea Surface Temperatures and their Influence on Southwestern US Hydroclimate

Colin Evans, Sloan Coats, Carlos M. Carrillo, Xiaolu Li, Marc Alessi, Dimitris A. Herrera, & Toby Ault

Biogenic amines for individual and social learning

Catherine Tait, Hasim Hakanoğlu, Akülkü Akülkü, Christopher Mayack & Dhruba Naug
Individuals learn about their environment through personal observation or by observing their social conspecifics. The speed-accuracy trade-off associated with these two strategies results in two possible learning phenotypes – slow but more accurate individual learners and fast but more inaccurate social learners. While the costs and benefits of these different learning strategies are well known, we know much less about the possible neurophysiological mechanisms that underlie such cognitive variation. Neurotransmitters, based on their important role...

Registration Year

  • 2022

Resource Types

  • Dataset


  • Colorado State University
  • US Forest Service
  • University of Colorado Boulder
  • University of Wyoming
  • Princeton University
  • Natural History Museum of Los Angeles County
  • United States Geological Survey
  • Cleveland State University
  • California State University, Northridge
  • University of Massachusetts Amherst