93 Works

Difficulties translating antisense-mediated activation of Frataxin expression from cell culture to mice

Audrius Kilikevicius, Jun Wang, Xiulong Shen, Frank Rigo, Thahza P. Prakash, Marek Napierala & David R. Corey
Friedreich’s ataxia (FA) is an inherited neurodegenerative disorder caused by decreased expression of frataxin (FXN) protein. Previous studies have shown that antisense oligonucleotides (ASOs) and single-stranded silencing RNAs can be used to increase expression of frataxin in cultured patient-derived cells. In this study, we investigate the potential for oligonucleotides to increase frataxin expression in a mouse model for FA. After confirming successful in vivo delivery of oligonucleotides using a benchmark gapmer targeting the nuclear noncoding...

Additional file 2 of Replication stress generates distinctive landscapes of DNA copy number alterations and chromosome scale losses

Nadeem Shaikh, Alice Mazzagatti, Simone De Angelis, Sarah C. Johnson, Bjorn Bakker, Diana C. J. Spierings, René Wardenaar, Eleni Maniati, Jun Wang, Michael A. Boemo, Floris Foijer & Sarah E. McClelland
Additional file 2. Contains CNAs for RPE1 after treatment with DMSO, Aphidicolin or siRNA.

Additional file 3 of Genetic diversity in the transmission-blocking vaccine candidate Plasmodium vivax gametocyte protein Pvs230 from the China–Myanmar border area and central Myanmar

Xin Zhao, Yubing Hu, Yan Zhao, Lin Wang, Zifang Wu, Myat Thu Soe, Myat Phone Kyaw, Liwang Cui, Xiaotong Zhu & Yaming Cao
Additional file 3: Table S3. Codon-based tests for selection on Pvs230 in China–Myanmar border and Myanmar isolates.

Additional file 4 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 4

Additional file 4 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 4

Additional file 6 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 6

Additional file 14 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 14

Additional file 14 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 14

Additional file 16 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 16

Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis

Jingru Bai, Tong Liu, Bo Tu, Meng Yuan, Zhaoqi Shu, Minghe Fan, Sihan Huo, Yuyao Guo, Lina Wang, Hua Wang & Ying Zhao
Cancer-associated fibroblasts (CAFs) are considered one of the most critical stromal cells that interact with pancreatic ductal adenocarcinoma (PDAC) and promote tumor growth, metastasis, and treatment resistance. Previous studies illustrated macroautophagy/autophagy contributes to CAF activation during tumor progression. Here in our study, we found that autophagy deficiency in CAFs impedes CAF activation by inhibiting proline biosynthesis and collagen production. Furthermore, we uncovered that autophagy promotes proline biosynthesis through mitophagy-mediated regulation of NADK2 (NAD kinase 2,...

Additional file 10 of Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy

Kai Sun, Dandan Li, Aoyun Xia, Hua Zhao, Qin Wen, Sisi Jia, Jiafeng Wang, Guili Yang, Danhua Zhou, Cuihong Huang, Hui Wang, Zhiqiang Chen & Tao Guo
Additional file 10: Table S7. Mutation site information screened by whole genome sequencing.

Additional file 5 of Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy

Kai Sun, Dandan Li, Aoyun Xia, Hua Zhao, Qin Wen, Sisi Jia, Jiafeng Wang, Guili Yang, Danhua Zhou, Cuihong Huang, Hui Wang, Zhiqiang Chen & Tao Guo
Additional file 5: Table S2. Primers for amplifying fragments of the target site.

Additional file 9 of Targeted Identification of Rice Grain-Associated Gene Allelic Variation Through Mutation Induction, Targeted Sequencing, and Whole Genome Sequencing Combined with a Mixed-Samples Strategy

Kai Sun, Dandan Li, Aoyun Xia, Hua Zhao, Qin Wen, Sisi Jia, Jiafeng Wang, Guili Yang, Danhua Zhou, Cuihong Huang, Hui Wang, Zhiqiang Chen & Tao Guo
Additional file 9: Table S6. Mutation site information obtained by targeted sequencing.

Additional file 2 of Sequential gene expression analysis of cervical malignant transformation identifies RFC4 as a novel diagnostic and prognostic biomarker

Jianwei Zhang, Silu Meng, Xiaoyan Wang, Jun Wang, Xinran Fan, Haiying Sun, Ruoqi Ning, Bing Xiao, Xiangqin Li, Yao Jia, Dongli Kong, Ruqi Chen, Changyu Wang, Ding Ma & Shuang Li
Additional file 2: Table S7. Enrichment of chromosomal region. Table S8. Simplified results of GO enrichment. Table S9. KEGG enrichment results.

Enhancement of the nitrogen-fixing activity of paddy soils owing to iron application

Yoko Masuda, Yutaka Shiratori, Hirotomo Ohba, Takanori Ishida, Ryo Takano, Sakura Satoh, Weishou Shen, Nan Gao, Hideomi Itoh & Keishi Senoo
Anaeromyxobacter and Geobacter, iron-reducing bacteria belonging to Deltaproteobacteria, are newly discovered nitrogen-fixing bacteria predominant in paddy soils. We hypothesized that adding ferric iron oxide as an electron acceptor for respiration of the iron-reducing bacteria could enhance the nitrogen-fixing activity of Anaeromyxobacter and Geobacter in paddy soils. In the soil microcosm study, soil nitrogen-fixing activity significantly increased after adding ferrihydrite or Fe2O3, ferric iron oxides, to the soil. In these soils, gene transcripts of nifD from...

Enhancement of the nitrogen-fixing activity of paddy soils owing to iron application

Yoko Masuda, Yutaka Shiratori, Hirotomo Ohba, Takanori Ishida, Ryo Takano, Sakura Satoh, Weishou Shen, Nan Gao, Hideomi Itoh & Keishi Senoo
Anaeromyxobacter and Geobacter, iron-reducing bacteria belonging to Deltaproteobacteria, are newly discovered nitrogen-fixing bacteria predominant in paddy soils. We hypothesized that adding ferric iron oxide as an electron acceptor for respiration of the iron-reducing bacteria could enhance the nitrogen-fixing activity of Anaeromyxobacter and Geobacter in paddy soils. In the soil microcosm study, soil nitrogen-fixing activity significantly increased after adding ferrihydrite or Fe2O3, ferric iron oxides, to the soil. In these soils, gene transcripts of nifD from...

Delivery of mesenchymal stem cells-derived extracellular vesicles with enriched miR-185 inhibits progression of OPMD

Lin Wang, Panpan Yin, Jiaqi Wang, Yuanyuan Wang, Zheng Sun, Yu Zhou & Xiaobing Guan
Oral leukoplakia is one of the most common oral potentially malignant disorders (OPMDs) and its malignant transformation to oral cancer is highly associated with chronic inflammation. Extracellular vesicles (EVs) or exosome-delivered microRNAs modulate inflammatory responses and alleviate irritations that predisposes to cancer. We previously reported that microRNA-185 (miR-185) was significantly decreased in the buccal tissue of patients with oral cancer. In this study, we utilized genetically modified mesenchymal stem cells (MSCs) derived EVs with high...

Cancer-associated fibroblast-released extracellular vesicles carrying miR-199a-5p induces the progression of​ gastric cancer through regulation of FKBP5-mediated AKT1/mTORC1 signaling pathway

Yan Wang, Tao Li, Lei Yang, Xunlei Zhang, Xiaoli Wang, Xiaoqin Su, Congfei Ji & Zhenxin Wang
Accumulating evidence has unfolded the significance of extracellular vesicles (EVs) in diseases and cancers. Here, we attempted to discuss the role of cancer-associated fibroblasts (CAFs)-derived EVs containing miR-199a-5p in gastric tumorigenesis. Upregulated miR-199a-5p was first identified in cancer cells. Then, we selected CAFs for isolation of EVs which were co-cultured with AGS cells. We observed successful delivery of miR-199a-5p via CAF-derived EVs. Besides, miR-199a-5p promoted malignant properties of AGS cells. Moreover, miR-199a-5p downregulated FKBP5, leading...

Additional file 3 of Genetic diversity in the transmission-blocking vaccine candidate Plasmodium vivax gametocyte protein Pvs230 from the China–Myanmar border area and central Myanmar

Xin Zhao, Yubing Hu, Yan Zhao, Lin Wang, Zifang Wu, Myat Thu Soe, Myat Phone Kyaw, Liwang Cui, Xiaotong Zhu & Yaming Cao
Additional file 3: Table S3. Codon-based tests for selection on Pvs230 in China–Myanmar border and Myanmar isolates.

Additional file 1 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 1

Additional file 1 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 1

Additional file 2 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 2

Additional file 2 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 2

Additional file 3 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 3

Additional file 9 of Comparison of anther transcriptomes in response to cold stress at the reproductive stage between susceptible and resistant Japonica rice varieties

Zhenhua Guo, Wendong Ma, Lijun Cai, Tao Guo, Hao Liu, Linan Wang, Junliang Liu, Bo Ma, Yanjiang Feng, Chuanxue Liu & Guojun Pan
Supplementary Material 9

Registration Year

  • 2023
    6
  • 2022
    81
  • 2021
    2
  • 2019
    4

Resource Types

  • Dataset
    93

Affiliations

  • Second Hospital of Tianjin Medical University
    93
  • Huazhong University of Science and Technology
    75
  • South China Agricultural University
    59
  • Sichuan University
    57
  • Southern Medical University
    57
  • Southwest University
    50
  • West China Second University Hospital of Sichuan University
    48
  • University of Southampton
    48
  • Peking University
    48
  • Guangzhou Medical University
    44