5 Works

Data from: Land abandonment and intensification diminish spatial and temporal β-diversity of grassland plants and herbivorous insects within paddy terraces

Kei Uchida & Atushi Ushimaru
1. Although traditional land-use and management practices are known to enhance environmental heterogeneity in agricultural lands, loss of heterogeneity resulting from recent land-use changes can lead to spatiotemporal β-diversity losses (biotic homogenization). However, patterns and causes of β-diversity loss, especially at a within-field scale, have remained unexplored. 2. We examined β-diversity declines in plants, butterflies and orthopterans resulting from land abandonment and intensification in semi-natural grasslands on paddy margins at a within-field scale in relation...

Data from: Host manipulation by an ichneumonid spider ectoparasitoid that takes advantage of preprogrammed web-building behaviour for its cocoon protection

Keizo Takasuka, Tomoki Yasui, Ishigami Toru, Kensuke Nakata, Rikio Matsumoto, Kenichi Ikeida & Kaoru Maeto
Host manipulation by parasites and parasitoids is a fascinating phenomenon within evolutionary ecology, representing an example of extended phenotypes. To elucidate the mechanism of host manipulation, revealing the origin and function of the invoked actions is essential. Our study focused on the ichneumonid spider ectoparasitoid Reclinervellus nielseni, which turns its host spider (Cyclosa argenteoalba) into a drugged navvy, to modify the web structure into a more persistent cocoon web so that the wasp can pupate...

Data from: High cell density upregulates calcium oscillation by increasing calcium store content via basal mitogen-activated protein kinase activity

Mitsuhiro Morita, Akira Nakane, Yuki Fujii, Shohei Maekawa & Yoshihisa Kudo
Calcium releases of non-excitable cells are generally a combination of oscillatory and non-oscillatory patterns, and factors affecting the calcium dynamics are still to be determined. Here we report the influence of cell density on calcium increase patterns of clonal cell lines. The majority of HeLa cells seeded at 1.5 x 104/cm2 showed calcium oscillations in response to histamine and ATP, whereas cells seeded at 0.5 x 104/cm2 largely showed transient and sustained calcium increases. Cell...

Data from: MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

Masaki Miya, Yukuto Sato, Tsukasa Fukunaga, Tetsuya Sado, Jan Y. Poulsen, Keiichi Sato, Toshifumi Minamoto, Satoshi Yamamoto, Hiroki Yamanaka, Hitoshi Araki, Michio Kondoh & Wataru Iwasaki
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome sequences from 160 elasmobranchs (sharks and rays). The primers target a hypervariable region of the 12S rRNA gene (163–185 bp), which contains sufficient information to identify fishes to taxonomic family, genus and species except for some closely related congeners. To test versatility...

Data from: A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan

Sou Fukumoto, Atushi Ushimaru & Toshifumi Minamoto
1. To prevent the invasion of exotic species causing a decline in an endangered endemic species, it is important to determine the distribution of both species at an early stage, when the density of the exotic species is still low, and to manage the invasion immediately. However, distinguishing between closely related species is difficult because they share similar characteristics. 2. The identification of DNA fragments sampled from a body of water (environmental DNA) has become...

Registration Year

  • 2015

Resource Types

  • Dataset


  • Kobe University
  • Osaka Museum of Natural History
  • Ryukoku University
  • Tohoku University
  • Hokkaido University
  • Kyoto University
  • Natural History Museum and Institute
  • University of Tokyo
  • Kyoto Women's University
  • Research Institute for Humanity and Nature