33 Works

Data from: Delayed transmission selects for increased survival of vesicular stomatitis virus

Brian R. Wasik, Ambika Bhushan, C. Brandon Ogbunugafor & Paul E. Turner
Life-history theory predicts that traits for survival and reproduction cannot be simultaneous maximized in evolving populations. For this reason, in obligate parasites such as infectious viruses, selection for improved between-host survival during transmission may lead to evolution of decreased within-host reproduction. We tested this idea using experimental evolution of RNA virus populations, passaged under differing transmission times in the laboratory. A single ancestral genotype of Vesicular Stomatitis Virus (VSV), a negative-sense RNA Rhabdovirus, was used...

Data from: Positive selection drives faster-Z evolution in silkmoths

Timothy B. Sackton, Russell B. Corbett-Detig, Javaregowda Nagaraju, R. Lakshmi Vaishna, Kallare P. Arunkumar & Daniel L. Hartl
Genes linked to X or Z chromosomes, which are hemizygous in the heterogametic sex, are predicted to evolve at different rates than those on autosomes. This “faster-X effect” can arise either as a consequence of hemizygosity, which leads to more efficient selection for recessive beneficial mutations in the heterogametic sex, or as a consequence of reduced effective population size of the hemizygous chromosome, which leads to increased fixation of weakly deleterious mutations due to genetic...

Data from: Basal metabolism in tropical birds: latitude, altitude, and the “pace of life”

Gustavo A. Londoño, Mark A. Chappell, María Del Rosario Castañeda, Jill E. Jankowski & Scott K. Robinson
1. Life history varies across latitudes, with the ‘pace of life’ being ‘slower’ in tropical regions. Because life history is coupled to energy metabolism via allocation tradeoffs and links between performance capacity and energy use, low metabolic intensity is expected in tropical animals. Low metabolism has been reported for lowland tropical birds, but it is unclear if this is due to ‘slow’ life history or to a warm, stable environment. 2. We measured Basal Metabolic...

Data from: Neighbouring-group composition and within-group relatedness drive extra-group paternity rate in the European badger (Meles meles)

Geetha Annavi, Chris Newman, Hannah L. Dugdale, Christina C. Buesching, Yung W. Sin, Terry Burke & David W. Macdonald
Extra-group paternity (EGP) occurs commonly among group-living mammals and plays an important role in mating systems and the dynamics of sexual selection; however, socio-ecological and genetic correlates of EGP have been underexplored. We use 23 years of demographic and genetic data from a high-density European badger (Meles meles) population, to investigate the relationship between the rate of EGP in litters and mate availability, mate incompatibility and mate quality (heterozygosity). Relatedness between within-group assigned mothers and...

Data from: Associations between changes in city and address specific temperature and QT interval - the VA Normative Aging Study

Amar J. Mehta, Itai Kloog, Antonella Zanobetti, Brent A. Coull, David Sparrow, Pantel Vokonas & Joel Schwartz
Background: The underlying mechanisms of the association between ambient temperature and cardiovascular morbidity and mortality are not well understood, particularly for daily temperature variability. We evaluated if daily mean temperature and standard deviation of temperature was associated with heart rate-corrected QT interval (QTc) duration, a marker of ventricular repolarization in a prospective cohort of older men. Methods: This longitudinal analysis included 487 older men participating in the VA Normative Aging Study with up to three...

Data from: Resolving conflict in eutherian mammal phylogeny using phylogenomics and the multispecies coalescent model

Liang Liu, Scott V. Edwards & Shaoyuan Wu
The reconstruction of the Tree of Life has relied almost entirely on concatenation methods, which do not accommodate gene tree heterogeneity, a property that simulations and theory have identified as a likely cause of incongruent phylogenies. However, this incongruence has not yet been demonstrated in empirical studies. Several key relationships among eutherian mammals remain controversial and conflicting among previous studies, including the root of eutherian tree and the relationships within Euarchontoglires and Laurasiatheria. Both Bayesian...

Data from: Comparison and optimization of hiPSC forebrain cortical differentiation protocols

Christina R. Muratore, Priya Srikanth, Dana G. Callahan & Tracy L. Young-Pearse
Several protocols have been developed for human induced pluripotent stem cell neuronal differentiation. We compare several methods for forebrain cortical neuronal differentiation by assessing cell morphology, immunostaining and gene expression. We evaluate embryoid aggregate vs. monolayer with dual SMAD inhibition differentiation protocols, manual vs. AggreWell aggregate formation, plating substrates, neural progenitor cell (NPC) isolation methods, NPC maintenance and expansion, and astrocyte co-culture. The embryoid aggregate protocol, using a Matrigel substrate, consistently generates a high yield...

Data from: An integrated approach reveals regulatory controls on bacterial translation elongation

Arvind R. Subramaniam, Brian M. Zid & Erin K. O'Shea
Ribosomes elongate at a nonuniform rate during translation. Theoretical models and experiments disagree on the in vivo determinants of elongation rate and the mechanism by which elongation rate affects protein levels. To resolve this conflict, we measured transcriptome-wide ribosome occupancy under multiple conditions and used it to formulate a whole-cell model of translation in E. coli. Our model predicts that elongation rates at most codons during nutrient-rich growth are not limited by the intracellular concentrations...

Registration Year

  • 2014
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Affiliations

  • Harvard University
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  • University of Oxford
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  • University of California, Davis
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  • University of Michigan-Ann Arbor
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  • University of Groningen
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  • Smithsonian Institution
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  • University of Florida
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