6 Works

Data from: Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso

Aissata Barry, Marije C. Behet, Issa Nébié, Kjerstin Lanke, Lynn Grignard, Alphonse Ouedraogo, Issiaka Soulama, Chris Drakeley, Robert Sauerwein, Judith M. Bolscher, Koen J. Dechering, Teun Bousema, Alfred B. Tiono & Bronner P. Gonçalves
Background: Individuals living in malaria-endemic regions develop naturally acquired immunity against severe malarial disease, but it is unclear whether immunity that affects the establishment of infections develops following continuous natural exposure. Methods: We cleared schoolchildren in Burkina Faso of possible sub-patent infections and examined them weekly for incident infections by PCR. Plasma samples collected at enrolment were used to quantify antibodies to the pre-eryhrocytic-stage antigens circumsporozoite protein (CSP) and liver stage antigen. Sporozoite gliding inhibition...

Plasmodium falciparum gametocyte density and infectivity in peripheral blood and skin tissue of naturally infected parasite carriers in Burkina Faso

Teun Bousema, Elamaran Meibalan, Aissata Barry, Matthew Gibbins, Shehu Awandu, Lisette Meerstein-Kessel, Fiona Achar, Selina Bopp, Christopher Moxon, Amidou Diarra, Siaka Debe, Nicolas Ouedraogo, Ines Barry-Some, Emilie Badoum, Traore Fagnima, Kjerstin Lanke, Bronner Goncalves, John Bradley, Dyann Wirth, Chris Drakeley, Wamdaogo Guelbeogo, Alfred Tiono, Matthias Marti, Matthew P Gibbins, Fiona Achcar … & Alfred B Tiono
Background Plasmodium falciparum transmission depends on mature gametocytes that can be ingested by mosquitoes taking a blood meal on human skin. Although gametocyte skin sequestration has long been hypothesized as important contributor to efficient malaria transmission, this has never been formally tested. Methods In naturally infected gametocyte carriers from Burkina Faso, we assessed infectivity to mosquitoes by direct skin feeding and membrane feeding. We directly quantified male and female gametocytes and asexual parasites in finger-prick...

Data from: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission.

André Lin Ouédraogo, Teun Bousema, Petra Schneider, Sake J. De Vlas, Edith Ilboudo-Sanogo, Nadine Cuzin-Ouattara, Issa Nébié, Will Roeffen, Jan Peter Verhave, Adrian J.F. Luty & Robert Sauerwein
Background: Man to mosquito transmission of malaria depends on the presence of the sexual stage parasites, gametocytes, that often circulate at low densities. Gametocyte densities below the microscopical threshold of detection may be sufficient to infect mosquitoes but the importance of submicroscopical gametocyte carriage in different transmission settings is unknown. Methodology/Principal Findings: Membrane feeding experiments were carried out on 80 children below 14 years of age at the end of the wet season in an...

Data from: Dissecting functional components of reproductive isolation among closely related sympatric species of the Anopheles gambiae complex

Marco Pombi, Pierre Kengne, Geoffrey Gimonneau, Billy Tene-Fossog, Diego Ayala, Colince Kamdem, Federica Santalomazza, Wamdaogo Moussa Guelbeogo, N'Falé Sagnon, Vincenzo Petrarca, Didier Fontenille, Nora J. Besansky, Christophe Antonio-Nkondjio, Roch K. Dabire, Alessandra Della Torre, Frederic Simard & Carlo Costantini
Explaining how and why reproductive isolation evolves and determining which forms of reproductive isolation have the largest impact on the process of population divergence are major goals in the study of speciation. By studying recent adaptive radiations in incompletely isolated taxa, it is possible to identify barriers involved at early divergence before other confounding barriers emerge after speciation is complete. Sibling species of the Anopheles gambiae complex offer opportunities to provide insights into speciation mechanisms....

Data from: Examining the human infectious reservoir for Plasmodium falciparum malaria in areas of differing transmission intensity

Bronner P. Gonçalves, Melissa C. Kapulu, Patrick Sawa, Wamdaogo M. Guelbéogo, Alfred B. Tiono, Lynn Grignard, Will Stone, Joel Hellewell, Kjerstin Lanke, Guido J. H. Bastiaens, John Bradley, Issa Nébié, Joyce M. Ngoi, Robin Oriango, Dora Mkabili, Maureen Nyaurah, Janet Midega, Dyann F. Wirth, Kevin Marsh, Thomas S. Churcher, Philip Bejon, Sodiomon B. Sirima, Chris Drakeley & Teun Bousema
A detailed understanding of the human infectious reservoir is essential for improving malaria transmission-reducing interventions. Here we report a multi-regional assessment of population-wide malaria transmission potential based on 1209 mosquito feeding assays in endemic areas of Burkina Faso and Kenya. Across both sites, we identified 39 infectious individuals. In high endemicity settings, infectious individuals were identifiable by research-grade microscopy (92.6%; 25/27), whilst one of three infectious individuals in the lowest endemicity setting was detected by...

Data from: The temporal dynamics and infectiousness of subpatent Plasmodium falciparum infections in relation to parasite density

Hannah C. Slater, Amanda Ross, Ingrid Felger, Natalie E. Hofmann, Leanne Robinson, Jackie Cook, Bronner P. Gonçalves, Anders Björkman, Andre Lin Ouedraogo, Ulrika Morris, Mwinyi Msellem, Cristian Koepfli, Ivo Mueller, Fitsum Tadesse, Endalamaw Gadisa, Smita Das, Gonzalo Domingo, Melissa Kapulu, Janet Midega, Seth Owusu-Agyei, Cécile Nabet, Renaud Piarroux, Ogobara Doumbo, Safiatou Niare Doumbo, Kwadwo Koram … & Lucy C. Okell
Malaria infections occurring below the limit of detection of standard diagnostics are common in all endemic settings. However, key questions remain surrounding their contribution to sustaining transmission and whether they need to be detected and targeted to achieve malaria elimination. In this study we analyse a range of malaria datasets to quantify the density, detectability, course of infection and infectiousness of subpatent infections. Asymptomatically infected individuals have lower parasite densities on average in low transmission...

Registration Year

  • 2021
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  • 2018
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  • 2017
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  • 2013
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Resource Types

  • Dataset
    6

Affiliations

  • Centre National de Recherche et de Formation sur le Paludisme
    6
  • London School of Hygiene & Tropical Medicine
    5
  • Radboud University Nijmegen Medical Centre
    3
  • Radboud University Nijmegen
    2
  • Medical Research Council
    2
  • Noguchi Memorial Institute for Medical Research
    1
  • University of Notre Dame
    1
  • University of Glasgow
    1
  • African Training and Research Centre in Administration for Development
    1
  • Erasmus University Medical Center
    1