2 Works

Data from: Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction

Anand Ranjan, Vu Q. Nguyen, Sheng Liu, Jan Wisniewski, Kim Jee Min, Xiaona Tang, Gaku Mizuguchi, Vivian Jou, Timothy J. Nickels, Brian P. English, Qinsi Zheng, Ed Luk, Timothee Lionnet, Luke D. Lavis, Carl Wu & Ejlal Elalaoui
The H2A.Z histone variant, a genome-wide hallmark of permissive chromatin, is enriched near transcription start sites in all eukaryotes. H2A.Z is deposited by the SWR1 chromatin remodeler and evicted by unclear mechanisms. We tracked H2A.Z in living yeast at single-molecule resolution, and found that H2A.Z eviction is dependent on RNA Polymerase II (Pol II) and the Kin28/Cdk7 kinase, which phosphorylates Serine 5 of heptapeptide repeats on the carboxy-terminal domain of the largest Pol II subunit...

The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis

Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada, Masaya Ono, Satomi Yogosawa & Kiyotsugu Yoshida
Mammalian Hedgehog (Hh) signaling plays key roles in embryogenesis and uniquely requires primary cilia. Functional analyses of several ciliogenesis-related genes led to the discovery of the developmental diseases known as ciliopathies. Hence, identification of mammalian factors that regulate ciliogenesis can provide insight into the molecular mechanisms of embryogenesis and ciliopathy. Here, we demonstrate that DYRK2 acts as a novel mammalian ciliogenesis-related protein kinase. Loss of Dyrk2 in mice causes suppression of Hh signaling and results...

Registration Year

  • 2020
    2

Resource Types

  • Dataset
    2

Affiliations

  • National Cancer Institute
    2
  • Howard Hughes Medical Institute
    1
  • Johns Hopkins University
    1
  • Jichi Medical University
    1
  • New York University
    1
  • Teikyo University
    1
  • Stony Brook University
    1