499 Works

Arabidopsis sucrose transporter 4 (AtSUC4) is involved in high sucrose-mediated inhibition of root elongation

Siwen Liu, Jianmei Long, Liding Zhang, Jiayu Gao, Tiantian Dong, Ying Wang & Changcao Peng
Sucrose transporters (SUCs/SUTs) play crucial roles in apoplast transport and long-distance distribution of sucrose throughout the whole plant. However, whether and how the Arabidopsis AtSUC4 modulates sucrose import from apoplast to cytosol remains unclear. In the present study, we found that AtSUC4 protein was localized to the plasma membrane in the root. Expression of AtSUC4 in roots was gradually induced with the increasing sucrose concentrations (0%, 2%, 4% and 6%). When feeding high concentrations (4%...

Cancer-associated fibroblast-released extracellular vesicles carrying miR-199a-5p induces the progression of​ gastric cancer through regulation of FKBP5-mediated AKT1/mTORC1 signaling pathway

Yan Wang, Tao Li, Lei Yang, Xunlei Zhang, Xiaoli Wang, Xiaoqin Su, Congfei Ji & Zhenxin Wang
Accumulating evidence has unfolded the significance of extracellular vesicles (EVs) in diseases and cancers. Here, we attempted to discuss the role of cancer-associated fibroblasts (CAFs)-derived EVs containing miR-199a-5p in gastric tumorigenesis. Upregulated miR-199a-5p was first identified in cancer cells. Then, we selected CAFs for isolation of EVs which were co-cultured with AGS cells. We observed successful delivery of miR-199a-5p via CAF-derived EVs. Besides, miR-199a-5p promoted malignant properties of AGS cells. Moreover, miR-199a-5p downregulated FKBP5, leading...

Table S10.xlsx from Transcriptome analysis and mining of genes related to shade tolerance in foxtail millet (Setaria italica (L.) P. Beauv.)

Dan Liu, Yanjiao Cui, Zilong Zhao, Jing Zhang, Suying Li & Zhengli Liu
A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Table S1.xlsx from Transcriptome analysis and mining of genes related to shade tolerance in foxtail millet (Setaria italica (L.) P. Beauv.)

Dan Liu, Yanjiao Cui, Zilong Zhao, Jing Zhang, Suying Li & Zhengli Liu
A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Table S3.xlsx from Transcriptome analysis and mining of genes related to shade tolerance in foxtail millet (Setaria italica (L.) P. Beauv.)

Dan Liu, Yanjiao Cui, Zilong Zhao, Jing Zhang, Suying Li & Zhengli Liu
A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Table S11.xlsx from Transcriptome analysis and mining of genes related to shade tolerance in foxtail millet (Setaria italica (L.) P. Beauv.)

Dan Liu, Yanjiao Cui, Zilong Zhao, Jing Zhang, Suying Li & Zhengli Liu
A stereo interplanting system with foxtail millet beneath chestnut trees is an effective planting method to raise the utilization of land in chestnut orchards, increase yields and improve quality of chestnut nuts. Consequently, exploration of genes involved in shade tolerance response in foxtail millet and breeding shade-tolerant varieties have become urgent issues. In this study, RNA-seq of leaf samples from two shade-tolerant varieties and three shade-intolerant varieties of foxtail millet at the booting stage was...

Additional file 12 of Evolution and function of developmentally dynamic pseudogenes in mammals

Sheng Hu Qian, Lu Chen, Yu-Li Xiong & Zhen-Xia Chen
Additional file 12: Table S11. Expression level of human DDPs in GTEx project.

Additional file 12 of Evolution and function of developmentally dynamic pseudogenes in mammals

Sheng Hu Qian, Lu Chen, Yu-Li Xiong & Zhen-Xia Chen
Additional file 12: Table S11. Expression level of human DDPs in GTEx project.

Additional file 4 of Evolution and function of developmentally dynamic pseudogenes in mammals

Sheng Hu Qian, Lu Chen, Yu-Li Xiong & Zhen-Xia Chen
Additional file 4: Table S3. Co-expression pair between pseudogenes and coding genes.

Additional file 5 of Evolution and function of developmentally dynamic pseudogenes in mammals

Sheng Hu Qian, Lu Chen, Yu-Li Xiong & Zhen-Xia Chen
Additional file 5: Table S4. GO enrichment for coding genes co-expressed with dynamic pseudogenes.

Sprouty4 is epigenetically upregulated in human colorectal cancer

Alexei J. Stuckel, Shuai Zeng, Zhen Lyu, Wei Zhang, Xu Zhang, Urszula Dougherty, Reba Mustafi, Tripti Khare, Qiong Zhang, Trupti Joshi, Marc Bissonnette & Sharad Khare
Sprouty4 (SPRY4) has been frequently reported as a tumor suppressor and is therefore downregulated in various cancers. For the first time, we report that SPRY4 is epigenetically upregulated in colorectal cancer (CRC). In this study, we explored DNA methylation and hydroxymethylation levels of SPRY4 in CRC cells and patient samples and correlated these findings with mRNA and protein expression levels. Three loci within the promoter region of SPRY4 were evaluated for 5mC levels in CRC...

Additional file 15 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 15: Supplementary Table 3. The results of Univariate Cox regression analysis for pan-cancer.

Development and validation of methods that enable high-quality droplet digital PCR and hematological profiling data from microvolume blood samples: Supplementary tables

Bin Xu & Ryan Haley
Aim: Mouse models have been crucial to preclinical studies in the increasingly relevant fields of cell and gene therapy. However, only small quantities of mouse blood can be collected without producing adverse physiological effects that compromise data integrity. Results: To address this limitation, two combined methods were developed to create detailed droplet digital PCR (ddPCR) and hematological profiles using only ∼20 μl of mouse blood. The validation of these methods, which can serve as a...

Additional file 1 of Functional structure analysis and genome-wide identification of CNX gene family in cotton

Nan Xu, Hong Zhang, Yuexin Zhang, Yapeng Fan, Jing Wang, Waqar Afzal Malik, Cun Rui, Mingge Han, Xuke Lu, Xiugui Chen, Junjuan Wang, Delong Wang, Shuai Wang, Chao Chen, Lixue Guo, Lanjie Zhao & Wuwei Ye
Additional file 1. Table S1: CNX family genes corresponding to four Gossypium species genes renamed.

Cytopenias following anti-CD19 chimeric antigen receptor (CAR) T cell therapy: a systematic analysis for contributing factors

Yuan Xia, Jue Zhang, Jing Li, Lina Zhang, Jianyong Li, Lei Fan & Lijuan Chen
Cytopenia is one of the most common adverse events following the CAR-T cell infusion, affecting the quality of life and potentially leading to life-threatening bleeding and infection. This study aimed to systematically review the cytopenias following anti-CD19 CAR-T therapy and further analyse the contributing factors. Databases including PubMed, MEDLINE, Embase and Cochrane were systematically searched on 8 May 2022. A random-effect meta-analysis was used to estimate the incidence of cytopenia, and subgroup analyses were applied...

Cytopenias following anti-CD19 chimeric antigen receptor (CAR) T cell therapy: a systematic analysis for contributing factors

Yuan Xia, Jue Zhang, Jing Li, Lina Zhang, Jianyong Li, Lei Fan & Lijuan Chen
Cytopenia is one of the most common adverse events following the CAR-T cell infusion, affecting the quality of life and potentially leading to life-threatening bleeding and infection. This study aimed to systematically review the cytopenias following anti-CD19 CAR-T therapy and further analyse the contributing factors. Databases including PubMed, MEDLINE, Embase and Cochrane were systematically searched on 8 May 2022. A random-effect meta-analysis was used to estimate the incidence of cytopenia, and subgroup analyses were applied...

Additional file 3 of Tumor microenvironment-associated lactate metabolism regulates the prognosis and precise checkpoint immunotherapy outcomes of patients with lung adenocarcinoma

Song Qiu, Ying Wang, Hui Rao, Qiuyang Que, Yanyang Wu, Rui Zhu, Xiaofei Feng, Jun Chi, Weiling Lai, Yihang Sun, Qi Xiao, Huaqiu Shi & Yi Xiang
Additional file 3: Raw documents and tables in the process of data analysis.

miR-342-5p inhibits osteosarcoma cell growth, migration, invasion, and sensitivity to Doxorubicin through targeting Wnt7b

Qing Liu, Zhenting Wang, Xiaohua Zhou, Mingying Tang, Wei Tan, Tianshi Sun & Youwen Deng
Osteosarcoma (OS) accounts for 9 percent of cancer-related deaths in young people. The PI3K/Akt signaling, a well-known carcinogenic signaling pathway in human cancer, cooperates with other signaling pathways such as Wnt signaling to promote cancer progression. Wnt7b, as a transforming member of the Wnt family, could activate mTORC1 through PI3K-AKT signaling and is upregulated in OS. In the present study, we found that miR-342-5p inhibits Wnt7b expression via direct binding to Wnt7b 3′-UTR. miR-342-5p overexpression...

Additional file 1 of CSP I-plus modified rEndostatin inhibits hepatocellular carcinoma metastasis via down-regulation of VEGFA and integrinβ1

Xueqin Chen, Yan Wang, Hancong Liu, Jingjing Zhang, Jie Wang, Xiaobao Jin & Yan Ma
Additional file 1: Supplementary Figures. The full-length blots of WB in Fig. 5B. Whole-cell extracts were resolved on 10% SDS-PAGE by bio-rad Mini-PROTEAN with 10 sample Wells (1Marker, 2space, 3Blank group, 4CSP I-plus group, 5Endostar group, 6rES-CSP group, 7rES-CSP+ heparin group, 8DDP group, 9space, 10Marker), and then electrotransferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore). The membranes were then probed with respective primary antibodies against VEGFA, MMP2, integrinβ1, and E-cadherin procured from Abcam, β-actin from...

Additional file 4 of Accuracy of next-generation sequencing for molecular profiling of small specimen of lung cancer: a prospective pilot study of side-by-side comparison

Xiaosong Ben, Dan Tian, Weitao Zhuang, Rixin Chen, Sichao Wang, Zihao Zhou, Cheng Deng, Ruiqing Shi, Songlin Liu, Dongkun Zhang, Jiming Tang, Liang Xie, Haiyu Zhou, Zhou Zhang, Min Li, Xuanye Zhang & Guibin Qiao
Additional file 4.

Additional file 3 of Functional analysis revealed the involvement of ZmABCB15 in resistance to rice black-streaked dwarf virus infection

Runqing Yue, Qi Sun, Jianguo Ding, Wenlan Li, Wencai Li, Meng Zhao, Shouping Lu, Tingru Zeng, Hua Zhang, Suxian Zhao, Shuanggui Tie & Zhaodong Meng
Additional file 3: Table S2. The CDSs of ZmABCB family.

Additional file 8 of Genome-wide identification, new classification, expression analysis and screening of drought & heat resistance related candidates in the RING zinc finger gene family of bread wheat (Triticum aestivum L.)

Yongliang Li, Pai Qin, Aolong Sun, Wenjun Xiao, Fenglin Chen, Yang He, Keyao Yu, You Li, Meng Zhang & Xinhong Guo
Additional file 8: Table S5. The list of primers.

Additional file 8 of Genome-wide identification, new classification, expression analysis and screening of drought & heat resistance related candidates in the RING zinc finger gene family of bread wheat (Triticum aestivum L.)

Yongliang Li, Pai Qin, Aolong Sun, Wenjun Xiao, Fenglin Chen, Yang He, Keyao Yu, You Li, Meng Zhang & Xinhong Guo
Additional file 8: Table S5. The list of primers.

Additional file 8 of ELOVL gene family plays a virtual role in response to breeding selection and lipid deposition in different tissues in chicken (Gallus gallus)

Dandan Wang, Xinyan Li, Panpan Zhang, Yuzhu Cao, Ke Zhang, Panpan Qin, Yulong Guo, Zhuanjian Li, Yadong Tian, Xiangtao Kang, Xiaojun Liu & Hong Li
Additional file 8: Table S6. All genomic SNPs in gene body and 2 kb promoter of ELOVL gene family used for PCA analysis.

Additional file 9 of ELOVL gene family plays a virtual role in response to breeding selection and lipid deposition in different tissues in chicken (Gallus gallus)

Dandan Wang, Xinyan Li, Panpan Zhang, Yuzhu Cao, Ke Zhang, Panpan Qin, Yulong Guo, Zhuanjian Li, Yadong Tian, Xiangtao Kang, Xiaojun Liu & Hong Li
Additional file 9: Table S7. Gene specific primers used for qRT-PCR.

Registration Year

  • 2022
    499

Resource Types

  • Dataset
    499

Affiliations

  • Southeast University
    499
  • Zhejiang University
    378
  • Fudan University
    334
  • Shanghai Jiao Tong University
    309
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    308
  • University of Chinese Academy of Sciences
    286
  • Chinese Academy of Sciences
    278
  • Sun Yat-sen University
    269
  • Sichuan University
    269
  • Capital Medical University
    250