778 Works

Morphology and phylogeny of two new Thalassiosiroid taxa (Bacillariophyceae), with two marginal rimoportulae

Biaobiao Niu, Yaqiong Guo, Nina Lundholm & Yang Li
Thalassiosira and related taxa i.e. the taxa within Thalassiosirales are among the most abundant and widespread diatoms. The vast majority have one marginal rimoportula, but very few always have two. The knowledge on Thalassiosiroid taxa in Chinese waters is restricted, and to explore the diversity, monoclonal strains were established from southern Chinese coastal waters. Morphological data were based on light and scanning electron microscopy. The D1‒D3 region of the ribosomal large subunit (LSU) and the...

Pyroptosis regulators exert crucial functions in prognosis, progression and immune microenvironment of pancreatic adenocarcinoma: a bioinformatic and in vitro research

Zhenghai Bai, Fangshi Xu, Xiaodan Feng, Yuan Wu, Junhua Lv, Yu Shi & Honghong Pei
Pyroptosis is an inflammatory programmed cell death, showing potentials to be a novel anti-cancer approach. However, the roles of pyroptosis-related (PR) genes (PRGs) in pancreatic adenocarcinoma (PAAD) remain elusive. In the present study, we constructed a novel PR risk signature through the lasso regression analysis. The risk signature was greatly conducive to PAAD prognostic assessment. PR risk score was identified as an independent prognostic factor and could distinguish the prognostic differences of most clinical subgroups....

Integrated bioinformatics and experiments reveal the roles and driving forces for HSF1 in colorectal cancer

Xiaomin Ren, Liyuan Zhang, Xiaolin Ma, Jiaqiu Li & Zhong Lu
Heat shock factor 1 (HSF1) has watershed significance in different tumors. However, the roles and driving forces for HSF1 in colorectal cancer (CRC) are poorly understood. Our study integrally analyzed the roles and driving forces for HSF1 in CRC by bioinformatics and experiments. The expression and prognostic characteristics of HSF1 were analyzed via UALCAN, GEPIA2, TISIDB, Prognoscan and HPA databases. Then, we analyzed the correlation between HSF1 expression and immune features via TIMER2 database. Subsequently,...

Plant elicitor peptide 1 fortifies root cell walls and triggers a systemic root-to-shoot immune signaling in Arabidopsis

Jie Zhang, Yuxi Li, Qixin Bao, Hongbo Wang & Shuguo Hou
Plant immunity is initiated by cell surface-localized receptors upon perception of pathogen-derived microbe or pathogen-associated molecular patterns (MAMPs/PAMPs), damage/danger-associated molecular patterns (DAMPs), and phytocytokines. Different patterns activate highly overlapping immune signaling at the early stage but divergent physiological responses at the late stage. Here, we indicate that plant elicitor peptide 1 (Pep1), a well-known DAMP, induces lignin and callose depositions, two types of late immune responses for strengthening the plant cell wall. Pep1-induced lignin and...

MERS-CoV nsp1 impairs the cellular metabolic processes by selectively downregulating mRNAs in a novel granules

Zhaoyi Pan, Yujie Feng, Zhihui Wang, Zhengyang Lei, Qiuju Han & Jian Zhang
MERS-CoV infection can damage the cellular metabolic processes, but the underlying mechanisms are largely unknown. Through screening, we found non-structural protein 1 (nsp1) of MERS-CoV could inhibit cell viability, cell cycle, and cell migration through its endonuclease activity. Transcriptome sequencing revealed that MERS-CoV nsp1 specifically downregulated the mRNAs of ribosomal protein genes, oxidative phosphorylation protein genes, and antigen presentation genes, but upregulated the mRNAs of transcriptional regulatory genes. Further analysis shown nsp1 existed in a...

Role of lncRNA FAM83H antisense RNA1 (FAM83H-AS1) in the progression of non-small cell lung cancer by regulating the miR-545-3p/heparan sulfate 6-O-sulfotransferase (HS6ST2) axis

Yue Zhang, Yue Yu, Xuchen Cao & Peng Chen
Long non-coding RNAs (lncRNAs) are crucial regulators of cancer pathogenesis and are potentially useful diagnostic and prognostic biomarker tools. FAM83H antisense RNA1 (FAM83H-AS1) has been reported to be a vital regulator of different cancers; however, little attention has been paid to its significance in lung cancer. Non-tumorigenic lung cell line BEAS-2B and adenocarcinoma lung cancer cell lines NCI-H1299 and HCC827 were used in the present study. In addition, RNA immunoprecipitation, Western blotting, quantitative reverse transcription-PCR...

Role of lncRNA FAM83H antisense RNA1 (FAM83H-AS1) in the progression of non-small cell lung cancer by regulating the miR-545-3p/heparan sulfate 6-O-sulfotransferase (HS6ST2) axis

Yue Zhang, Yue Yu, Xuchen Cao & Peng Chen
Long non-coding RNAs (lncRNAs) are crucial regulators of cancer pathogenesis and are potentially useful diagnostic and prognostic biomarker tools. FAM83H antisense RNA1 (FAM83H-AS1) has been reported to be a vital regulator of different cancers; however, little attention has been paid to its significance in lung cancer. Non-tumorigenic lung cell line BEAS-2B and adenocarcinoma lung cancer cell lines NCI-H1299 and HCC827 were used in the present study. In addition, RNA immunoprecipitation, Western blotting, quantitative reverse transcription-PCR...

Prevalence of major morbidities and outcome of all hospitalized neonates. A retrospective cohort study of Huai’an neonatal survivals

Yaling Xu, Xiaoqin Zhu, Hui Wang, Zhaojun Pan, Xiaoqiong Li, Xiaojing Guo, Hongni Yue & Bo Sun
Reliable data for causal implication of neonatal survival in China are lacking. We assumed that by analyzing surviving data of in-hospital neonatal care based on all livebirths in Huai’an, prevalence of neonatal morbidity, mortality and causal relations may be derived comprehensively. Data of all regionally hospitalized neonates were retrospectively linked with corresponding whole livebirths (59,056) in 2015 as a cohort. Diagnoses of diseases and causes of deaths were redefined and categorized. Disease patterns, prevalence of...

Prostate-specific membrane antigen modulates the progression of prostate cancer by regulating the synthesis of arginine and proline and the expression of androgen receptors and Fos proto-oncogenes

Xi Hong, Liang Mao, Luwei Xu, Qiang Hu & Ruipeng Jia
The expression of prostate-specific membrane antigen (PSMA) is strikingly upregulated during oncogenesis and prostate cancer (PCa) progression, but the functions of this antigen in PCa remain unclear. Here, we constructed PSMA-knockdown LNCaP and 22rv1 cell lines and performed metabonomic and transcriptomic analyses to determine the effects of PSMA on PCa metabolism and transcription. The metabolism of arginine and proline was detected using specific kits. The mRNA and protein expression levels of the identified differentially expressed...

Mesozoic tectonic transition of the northeastern North China Craton: Evidence from adakitic rocks in southeastern Jilin Province, China

Yu-Song Chen, Zheng-Hong Liu, Qing-Bin Guan, Le Wan, Jin Liu & Tian-Yu Gao
Chronological and geochemical studies of adakites and adakitic rocks are important in understanding the tectonic evolution and geodynamic processes. We present new zircon U–Pb ages, Hf isotope, and geochemical analyses of adakitic rocks exposed in the Baishan area, southeastern Jilin Province, China. These new ages, together with existing age data, indicate that adakitic magmatism in southeastern Jilin Province can be subdivided into four stages: Early–Middle Triassic (251–235 Ma), Late Triassic (221–219 Ma), late Early–early Late...

Genome-wide identification and expression pattern analysis of the TCP transcription factor family in Ginkgo biloba

Li Yu, Qiangwen Chen, Jiarui Zheng, Feng Xu, Jiabao Ye, Weiwei Zhang, Yongling Liao & Xiaoyan Yang
Plant-specific TCP transcription factors play an essential role in plant growth and development. They can regulate leaf curvature, flower symmetry and the synthesis of secondary metabolites. The flavonoids in Ginkgo biloba leaf are one of the main medicinally bioactivate compounds, which have pharmacological and beneficial health effects for humans. In this study, a total of 13 TCP genes were identified in G. biloba, and 5 of them belonged to PCF subclades (GbTCP03, GbTCP07, GbTCP05, GbTCP13,...

Offshore Island Connection Line: A new perspective of coastal urban development boundary simulation and multi-scenario prediction

Fei He, Jun Yang, Yuqing Zhang, Dongqi Sun, Li Wang, Xiangming Xiao & Jianhong Xia
The urban development boundary (UDB) is a core issue in territorial spatial planning and remains a research focus in urban development. However, in the past, land use simulations of coastal cities have had some limitations. For example, new urban land outside the research boundary could not be effectively simulated, resulting in a large gap between the simulation results and reality. In this study, an artificial neural network (ANN) cellular automata (CA) model based on a...

Genetic and pathogenic variation of Botrytis cinerea, the causal agent of grey mould on Panax ginseng in China

Rong Wang, Kun Liu, Bingwei Chen, Wanlong Ding & Yong Li
Grey mould, caused by Botrytis cinerea, is an increasingly destructive disease of ginseng (Panax ginseng) in China. However, the extent of variation in the morphological characteristics of fungal colonies, genetic background and pathogenicity of different strains of this pathogen remains unknown. In this study, we isolated and identified 102 isolates of B. cinerea from ginseng. Among these, the sclerotial phenotype was dominant, followed by the mycelial and conidial phenotypes, with the proportion of each phenotype...

Subspace Estimation with Automatic Dimension and Variable Selection in Sufficient Dimension Reduction

Jing Zeng, Qing Mai & Xin Zhang
Sufficient dimension reduction (SDR) methods target finding lower-dimensional representations of a multivariate predictor to preserve all the information about the conditional distribution of the response given the predictor. The reduction is commonly achieved by projecting the predictor onto a low-dimensional subspace. The smallest such subspace is known as the Central Subspace (CS) and is the key parameter of interest for most SDR methods. In this article, we propose a unified and flexible framework for estimating...

Diversity and profiles of volatile compounds in twenty-five peppermint genotypes grown in China

Lin Lu, Hua Cao, Han Li, Hao Zhang, Shenchong Li & Jihua Wang
Peppermint (Mentha piperita L.) is one of the most cultivated and consumed herbs. The pharmaceutical properties and nutrition values of peppermint oil make it an important commercial product. This study aimed to explore the volatile compounds in the essential oil extracted from different peppermint genotypes commercially grown in China. Oil extraction using a steam distillation followed by gas chromatography-mass spectrometry resulted in the quantification of 53 volatile compounds from five major classes, including esters, alcohols,...

Diversity and profiles of volatile compounds in twenty-five peppermint genotypes grown in China

Lin Lu, Hua Cao, Han Li, Hao Zhang, Shenchong Li & Jihua Wang
Peppermint (Mentha piperita L.) is one of the most cultivated and consumed herbs. The pharmaceutical properties and nutrition values of peppermint oil make it an important commercial product. This study aimed to explore the volatile compounds in the essential oil extracted from different peppermint genotypes commercially grown in China. Oil extraction using a steam distillation followed by gas chromatography-mass spectrometry resulted in the quantification of 53 volatile compounds from five major classes, including esters, alcohols,...

NLRC5 restricts dengue virus infection by promoting the autophagic degradation of viral NS3 through E3 ligase CUL2 (cullin 2)

Jiawei Hao, Jinqian Li, Zhenzhen Zhang, Yang Yang, Qing Zhou, Tiantian Wu, Tongling Chen, Zhongdao Wu, Ping Zhang, Jun Cui & Yi-Ping Li
NLRC5 has been reported to be involved in antiviral immunity; however, the underlying mechanism remains poorly understood. Here, we investigated the functional role of NLRC5 in the infection of a flavivirus, dengue virus (DENV). We found that the expression of NLRC5 was strongly induced by virus infection and IFNB or IFNG stimulation in different cell lines. Overexpression of NLRC5 remarkably suppressed DENV infection, whereas knockout of NLRC5 led to a significant increase in DENV infection....

Platelet–Lymphocyte Ratio, Neutrophil–Lymphocyte Ratio and Their Dynamic Changes with Type 2 Diabetes Mellitus: A Cohort Study in China

Canjia Zhang, Huan Chen, Shangxin Cui, Yeli Lin, Yongqiang Liang, Ping Zhao, Changyi Wang, Shan Xu, Xiaolin Peng, Hongen Chen, Li Wang, Dan Zhao, Ming Zhang, Dongsheng Hu, Yanmei Lou & Fulan Hu
This study aimed at investigating the relationships between Platelet-Lymphocyte ratio (PLR) and Neutrophil–Lymphocyte ratio (NLR) and their dynamic changes (∆PLR, ∆NLR) with type 2 diabetes mellitus (T2DM) in a Chinese cohort study. This study recruited 41,439 individuals who were diagnosed without T2DM at first health examination and completed at least one follow-up. The relationships between NLR, PLR, ∆PLR, ∆NLR and T2DM risk were analyzed using the Cox regression model with corresponding Hazard Ratios (HRs) and...

Utilizing network pharmacological analysis to investigate the key targets and mechanisms of kaempferol against oxaliplatin-induced neurotoxicity

Hongxing Wang, Jing Quan, Youming Deng, Jie Chen, Ke Zhang & Zhan Qu
This study investigated the pharmacological mechanism of kaempferol in the treatment of oxaliplatin-induced neuropathic pain by network pharmacological method and cells experiment. The kaempferol and disease target genes were obtained from several databases, including TCMSP, SwissTargetPrediction, GeneCards, and CTD. Then, the common target genes of drugs and diseases were obtained using Venny online tools. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses were carried out to obtain the enriched molecular...

The hsa_circRNA_102049 mediates the sorafenib sensitivity of hepatocellular carcinoma cells by regulating Reelin gene expression

Shaolei Wang, Dehua Liu, Hong Wei, Yang Hua, Guodong Shi & Jinhan Qiao
A growing body of research has illuminated that non-coding RNAs (ncRNAs) plays an important role in the development of drug resistance in hepatocellular carcinoma (HCC) cells. The expression profiles of differential expressed genes (DEGs) and ncRNAs related to the sorafenib resistance in HCC cells were analyzed according to the Gene Expression Omnibus (GEO) dataSets and The Cancer Genome Atlas (TCGA) datasets. Bioinformatics technology was used to construct the interaction network of DEGs and ncRNAs. Cell...

Clinical features of the first critical case of acute encephalitis caused by the avian influenza A (H5N6) virus

Libing Zhang, Kaituo Liu, Qin Su, Xiao Chen, Xiaoquan Wang, Qingfeng Li, Wenlei Wang, Xuhua Mao, Jinmei Xu, Xin Zhou, Qin Xu, Le Zhou, Xiufan Liu & Pinghu Zhang
Highly pathogenic avian influenza viruses (HPAIV), such as H5N1, H5N6, and H7N9, have been reported to frequently infect humans, but acute encephalitis caused by HPAIV in humans has been rarely reported. We report the first critical case of acute encephalitis with mild pneumonia caused by the H5N6 virus. On January 25 of 2022, a 6-year-old girl with severe neurological symptoms was admitted to our hospital and rapidly developed into seizures and coma. Brain imaging showed...

Transcriptome analysis identifies key genes involved in anthocyanin biosynthesis in black and purple fruits (Lycium ruthenicum Murr. L)

Xuemei Bao, Xiaolong Gan, Guanghui Fan, Guangrui Liu, Xiaolin Ma, Baolong Liu & Yuan Zong
Lycium ruthenicum is a characteristic plant resource in Northwest China. The fruit with high anthocyanin content is the main reason for its high nutritional value and economic value, while the regulation of key genes regulating anthocyanin biosynthesis in black fruit is not clear. In this study, high-throughput sequencing technology was used to compare the expression differences of all genes in black and purple fruits. The purple samples obtained 45.13 and 51.49 M Raw reads, while...

MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone

Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang & Zhaoqun Deng
This study aimed to explore the specific effect of miR-200c in anaplastic thyroid cancer (ATC). Hth74 and ARO cell lines were used. Proliferation, invasion, and colony formation activities of Hth74 and ARO cell lines affected by miR-200c were studied. Expression of epithelial-to-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, Slug, and Snail) in the Hth74 and ARO cell lines were validated by western blot and qRT-PCR. In addition, the regulation of the parathyroid hormone-like hormone (PTHLH) by...

MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone

Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang & Zhaoqun Deng
This study aimed to explore the specific effect of miR-200c in anaplastic thyroid cancer (ATC). Hth74 and ARO cell lines were used. Proliferation, invasion, and colony formation activities of Hth74 and ARO cell lines affected by miR-200c were studied. Expression of epithelial-to-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, Slug, and Snail) in the Hth74 and ARO cell lines were validated by western blot and qRT-PCR. In addition, the regulation of the parathyroid hormone-like hormone (PTHLH) by...

Dual-imaging-mode smart hydrogel information platform for illumination-independent covert decryption and read

Junjie Wei, Long Li, Rui Li, Qingquan Liu, Zejun Yan & Tao Chen
Smart hydrogel with color responsiveness is envisioned as one of the most promising materials for advanced information encryption and decryption platform, but the illumination-dependent way of decrypting and reading information leads to the worrying of concealment in some particular scenarios. Herein, we proposed a smart hydrogel information platform with dual imaging modes by utilizing the accompanying behaviors in transparency change and heat releasing after crystallization of supercooled solution. For this smart hydrogel information platform, the...

Registration Year

  • 2022
    778

Resource Types

  • Dataset
    778

Affiliations

  • Jiangsu Province Hospital
    778
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    529
  • Huazhong University of Science and Technology
    493
  • Sichuan University
    489
  • Shanghai Jiao Tong University
    471
  • Zhejiang University
    461
  • Fudan University
    424
  • Capital Medical University
    423
  • Nanjing Medical University
    406
  • Southern Medical University
    349