34 Works

Effects of an alternative host on the prevalence and infection intensity of a bumble bee parasite

Rebecca Irwin & Simon Pinilla-Gallego
Several bee parasites are transmitted through flowers, and some of them can infect multiple host species. Given the shared use of flowers by bee species, parasites can potentially encounter multiple host species, which could affect the evolution of parasite virulence. We used the trypanosomatid parasite Crithidia bombi and its host, the common eastern bumble bee (Bombus impatiens), to explore the effect of infecting an alternative host, the alfalfa leaf-cutter bee (Megachile rotundata), on parasite infectivity...

Life-history stage and the population genetics of the tiger mosquito Aedes albopictus at a fine spatial scale

Emily Reed, Michael Reiskind & Martha Burford Reiskind
As a widespread vector of disease, the mosquito species Aedes albopictus Skuse (Diptera: Culicidae) is a high priority for both public health and invasive species research and management. Like all mosquitoes, A. albopictus has a complex life history with aquatic egg, larval, and pupal stages and a terrestrial adult stage. This requires targeted management strategies for each life stage, coordinated across time and space. Researchers use population genetics to inform control of A. albopictus. However,...

Floral shape predicts bee-parasite transmission potential

Rebecca Irwin, Mario Pinilla-Gallego, Wee-Hao Ng & Victoria Amaral
The spread of parasites is one of the primary drivers of population decline of both managed and wild bees. Several bee parasites are transmitted by the shared use of flowers, turning floral resources into potential disease hotspots. However, we know little about how floral morphology and floral species identity affect different steps of the transmission process. Here, we used the gut parasite Crithidia bombi and its primary host, bumble bees (Bombus spp.), to examine whether...

Probing the molecular basis of fruit firmness in southern highbush blueberry (Vaccinium corymbosum hybrid) through RNA sequencing

Lena Wilson & Hamid Ashrafi
Blueberries (Vaccinium corymbosum L.) benefit from increased fruit firmness because of consumer preference and machine harvestability. However, the genetic component of fruit texture and skin thickness and their relationship to firmness have yet to be deciphered. This study used bulked segregant RNA-seq (BSR-seq) for differential gene expression analysis. Previously an F1 population of a cross between firm-fruited southern highbush cv. 'Reveille' and soft-fruited cv. 'Arlen' was developed in our laboratory. The total RNA of the...

Data from: AgMate: an optimal mating software versus other mate pair designing methods on long-term breeding of Pinus taeda L

Khushi Goda & Fikret Isik
Breeding objectives aim to optimize two crucial but contrasting goals of maximizing genetic gain while managing genetic diversity. In advanced generations, this becomes a challenge in monoecious conifer tree species breeding programs because they suffer from inbreeding. Developing an algorithm that maximizes genetic gain while maintaining genetic diversity for monoecious species is imperative. While methods and algorithms for animal breeding are well-established, an efficient algorithm suited to monoecious species remains elusive. Towards this goal, we...

Data: Rapid evolution of an adaptive taste polymorphism disrupts courtship behavior

Ayako Wada-Katsumata, Eduardo Hatano, Samantha McPherson, Jules Silverman & Coby Schal
The evolution of adaptive behavior often requires changes in sensory systems. However, rapid adaptive changes in sensory traits can adversely affect other fitness-related behaviors. In the German cockroach, a gustatory polymorphism, ‘glucose-aversion (GA)’, supports greater survivorship under selection with glucose-containing insecticide baits and promotes the evolution of behavioral resistance. Yet, sugars are prominent components of the male’s nuptial gift and play an essential role in courtship. Behavioral and chemical analyses revealed that the saliva of...

Data from: Seed fate in ant-mediated dispersal: Seed dispersal effectiveness in the Ectatomma ruidum (Formicidae) - Zanthoyxlum ekmanii (Rutaceae) system

Selina A. Ruzi & Andrew V. Suarez.
Plants are often dispersal limited relying on passive or active agents to find suitable microhabitats for germination. Seeds of pioneer tree species, for example, require light gaps for growth but have short median dispersal distances and often do not provide a food reward to encourage animal dispersal. Zanthoxylum ekmanii seeds are frequently moved by ants but evaluating the effectiveness of ant-mediated seed removal requires knowledge of the species moving the seeds, how far they are...

Resolving the phylogenetic relationship among recently diverged members of the rockfish subgenus Sebastosomus

Martha Burford Reiskind, Emma Wallace, Emily Reed & Andres Aguilar
Rapid speciation is an important aspect of adaptive radiations, but can obfuscate phylogenetic relationships among taxa. For recent radiations, there are challenges to reconstructing the relationships among the species due to often shorter branch lengths. Resolution of these relationships is further confounded when studies only use a few genetic markers. Double digest restriction-site associated DNA sequencing (ddRADseq) is a method of next generation sequencing that identifies many single nucleotide polymorphisms (SNPs) throughout the genome. This...

Consuming sunflower pollen reduced pathogen infection but did not alter measures of immunity in bumble bees

Alison Fowler, Ben Sadd, Toby Bassingthwaite, Rebecca Irwin & Lynn Adler
Certain diets can benefit bee health by reducing pathogens, but the mechanism(s) driving these medicinal effects are largely unexplored. Recent research found that sunflower (Helianthus annuus) pollen reduces the gut pathogen Crithidia bombi in the common eastern bumble bee (Bombus impatiens). Here, we tested the effects of sunflower pollen and infection on two bee immune metrics to determine if sunflower pollen diet drives changes in host immunity that can explain this medicinal effect. Bees were...

Diet nutritional condition affects tergal gland secretion and courtship success of male cockroaches

Samantha McPherson, Ayako Wada-Katsumata, Eduardo Hatano, Jules Silverman & Coby Schal
An integral part of the courtship sequence of the German cockroach (Blattella germanica) involves the male raising his wings to expose tergal glands on his dorsum. When a female cockroach feeds on the secretion of these glands, she is optimally positioned for mating. Core chemical components have been identified, but the effect of male diet on the tergal gland secretion remains unexplored. After validating the pivotal role of tergal feeding in mating, we starved or...

Parasites, niche modification and the host microbiome: A field survey of multiple parasites

Kayleigh R. O'Keeffe, Fletcher W. Halliday, Corbin D. Jones, Ignazio Carbone & Charles E. Mitchell
Parasites can affect and be affected by the host's microbiome, with consequences for host susceptibility, parasite transmission, and host and parasite fitness. Yet, two aspects of the relationship between parasite infection and host microbiota remain little understood: the nature of the relationship under field conditions, and how the relationship varies among parasites. To overcome these limitations, we performed a field survey of the within-leaf fungal community in a tall fescue population. We investigated how diversity...

Food for thought: Barro Colorado Island frugivore home range summaries

Shauhin Alavi, Roland Kays, Ben Hirsch, Rasmus Havmøller, Damien Caillaud & Margaret Crofoot
This dataset consists of home-range area summaries of four species of frugivores on Barro Colorado Island, Panama, including 12 kinkajou (Potos flavus), 16 white-nosed coati (Nasua narica), 8 white-faced capuchin monkey (Cebus capucinus), and 8 spider monkey (Ateles geoffroyi). Summaries include effective sample sizes (DOF) as well as parameter estimates (in hectares) including upper and lower confidence intervals. Metadata for each individual are provided including their indivdual ID and species ID. The summaries of the...

Phylogenomics and biogeography of Torreya (Taxaceae) – Integrating data from three organelle genomes, morphology, and fossils and a practical method for reducing missing data from RAD-seq

Wenbin Zhou, AJ Harris &
Restriction site-associated DNA sequencing (RAD-seq) enables obtaining thousands of genetic markers for phylogenomic studies. However, RAD-seq data are subject to allele dropout (ADO) due to polymorphisms at enzyme cutting sites. We developed a new pipeline, RADADOR, to mitigate the ADO in outgroups by recovering missing loci from previously published transcriptomes in our study of a gymnosperm genus Torreya. Using the supplemented RAD-seq data in combination with plastome and mitochondrial gene sequences, morphology, and fossil records,...

Data from: Estimating bee abundance: Can mark-recapture methods validate common sampling protocols?

Emma Briggs, Christopher Baranski, Olivia Münzer Schaetz, Gabriela Garrison, Jaime Collazo & Elsa Youngsteadt
Wild bees can be essential pollinators in natural, agricultural, and urban systems, but populations of some species have declined. Efforts to assess the status of wild bees are hindered by uncertainty in common sampling methods, such as pan traps and aerial netting, which may or may not provide a valid index of abundance across species and habitats. Mark-recapture methods are a common and effective means of estimating population size, widely used in vertebrates but rarely...

Reservoir water quality data

Carol Kinder & JoAnn Burkholder
Potable source-water reservoirs are the main water supplies in many urbanizing regions, yet their long-term responses to cultural eutrophication are poorly documented in comparison to natural lakes, creating major management uncertainties. Here, long-term discrete data (June 2006–June 2018) for classical eutrophication water quality indicators, continuous depth-profile data for dissolved oxygen (DO), and an enhanced hybrid statistical trend analysis model were used to evaluate the eutrophication status of a potable source-water reservoir. Based on classical indicators...

Host preferences inhibit transmission from potential superspreader host species

Skylar Hopkins, Cari McGregor, Lisa Belden & Jeremy Wojdak
Host species that are particularly abundant, infectious, and/or infected tend to contribute disproportionately to symbiont (parasite or mutualist) maintenance in multi-host systems. Therefore, in a facultative multi-host system where two host species had high densities, high symbiont infestation intensities, and high infestation prevalence, we expected interspecific transmission rates to be high. Instead, we found that interspecific symbiont transmission rates to caged sentinel hosts were an order of magnitude lower than intraspecific transmission rates in the...

Data from: Modeling spatiotemporal abundance and movement dynamics using an integrated spatial capture-recapture movement model

Eric Regehr, Ryan Wilson & Nathan Hostetter
Animal movement is a fundamental ecological process affecting the survival and reproduction of individuals, the structure of populations, and the dynamics of communities. Methods to quantify animal movement and spatiotemporal abundances, however, are generally separate and thus omit linkages between individual-level and population-level processes. We describe an integrated spatial capture-recapture (SCR) movement model to jointly estimate (1) the number and distribution of individuals in a defined spatial region and (2) movement of those individuals through...

Targeted sequencing of T-DNA borders in OCP1xOGC transgenic lines of Camelina

Brianne Edwards
Background: Genetic engineering of crop plants has been successful in transferring traits into elite lines beyond what can be achieved with breeding techniques. Introduction of transgenes originating from other species has conferred resistance to biotic and abiotic stresses, increased efficiency, and modified developmental programs. The next challenge is now to combine multiple transgenes into elite varieties via gene stacking to combine traits. Generating stable homozygous lines with multiple transgenes requires selection of segregating generations which...

Data for: Citizen science as an ecosystem of engagement: Implications for learning and broadening participation

Bradley Allf
The bulk of research on citizen science participants is project-centric, based on an assumption that volunteers experience a single project. Contrary to this assumption, survey responses (n=3,894) and digital trace data (n=3,649) from volunteers, who collectively engaged in 1,126 unique projects, revealed that multi-project participation was the norm. Only 23% of volunteers were singletons (who participated in only one project), and multi-project participants split evenly between disciplines specialists (39%) and discipline spanners (38% joined projects...

Phased, chromosome-scale genome assemblies of tetraploid potato reveals a complex genome, transcriptome, and predicted proteome landscape underpinning genetic diversity

Genevieve Hoopes, Xiaoxi Meng, John P. Hamilton, Sai Reddy Achakkagari, Fernanda De Alves Freitas Guesdes, Marie E. Bolger, Joseph J. Coombs, Danny Esselink, Natalie R. Kaiser, Linda Kodde, Maria Kyriakidou, Brian Lavrijssen, Natascha Van Lieshout, Rachel Shereda, Heather K. Tuttle, Brieanne Vaillancourt, Joshua C. Wood, Jan M. De Boer, Nolan Bornowski, Peter Bourke, David Douches, Herman J. Van Eck, Dave Ellis, Max J. Feldman, Kyle M. Gardner … & Richard Finkers
Hoopes G., Meng X., Hamilton J.P., Achakkagari S.R., de Alves Freitas Guesdes F., Bolger M.E., Coombs J.J., Esselink D., Kaiser N.R., Kodde L., Kyriakidou M., Lavrijssen B., van Lieshout N., Shereda R., Tuttle H.K., Vaillancourt B., Wood J.C., de Boer J.M., Bornowski N., Bourke P., Douches D., van Eck H.J., Ellis D., Feldman M.J., Gardner K.M., Hopman J.C.P., Jiang J., De Jong W.S., Kuhl J.C., Novy R.G., Oome S., Sathuvalli V., Tan E.H., Ursum R.A.,...

Data accompanying: Performance characterization of low-cost air sensors for off-grid deployment in rural Malawi

Ashley Bittner, Eben Cross, David Hagan, Carl Malings, Eric Lipsky & Andrew Grieshop
Low-cost gas and particulate sensor packages offer a compact, lightweight, and easily transportable solution to address global gaps in air quality (AQ) observations. However, regions that would benefit most from widespread deployment of low-cost AQ monitors often lack the reference grade equipment required to reliably calibrate and validate them. In this study, we explore approaches to calibrating and validating three integrated sensor packages before a one year deployment to rural Malawi using collocation data collected...

Investigating the relationship between polarimetric radar signatures of hydrometeor size sorting and tornadic potential in simulated supercells

S. Loeffler, M.R. Kumjian, P. Markowski, B.E. Coffer & M.D. Parker
The national upgrade of the operational weather radar network to polarimetric capabilities has lead to numerous studies focusing on polarimetric radar signatures commonly observed in supercells. One such signature is the horizontal separation of regions of enhanced differential reflectivity (ZDR) and specific differential phase (KDP) values due to hydrometeor size sorting. Recent observational studies have shown that the orientation of this separation tends to be more perpendicular to storm motion in supercells that produce tornadoes....

Sunflower plantings reduce a common gut pathogen and increase queen production in bumble bee colonies

Rosemary L. Malfi, Quinn S. McFrederick, Giselle Lozano, Rebecca E. Irwin & Lynn Adler
We evaluated whether plantings of sunflower (Helianthus annuus), whose pollen reduces infection by some pathogens when fed to bees in captivity, lowered pathogen levels and increased reproduction in free-foraging bumble bee colonies (Bombus impatiens). We placed pairs of commercial colonies of B. impatiens at 20 mixed vegetable farms in western Massachusetts between Jul-23 and Oct-6 2019. Flowering resources typically visited by bumble bees were quantified at each farm twice to characterize abundance and diversity. We...

Human-mediated trophic mismatch between fire, plants, and herbivores

Marcus Lashley, Michael Chitwood, Jacob Dykes, Christopher DePerno & Christopher Moorman
Trophic mismatches are commonly reported across a wide array of taxa and can have important implications for species participating in the interaction. However, to date, examples of trophic mismatch have centrally focused on those induced by shifts in climate. Here we report on the potential for humans to induce trophic mismatch by shifting the phenology of fire. Globally, anthropogenic fire ignitions are phenologically mismatched to that of historic lightning ignitions but the effects of this...

Not all trees can make a forest: tree species composition and competition control forest encroachment in a tropical savanna

Samuel Flake, Eliane Honda, Natashi Pilon, William Hoffmann & Giselda Durigan
Forest encroachment into savannas is a widespread phenomenon, the rate of which may depend on soil conditions, species composition, or changes in stand structure. As savanna specialist trees are replaced by generalist species, rates of stand development may increase. Because generalists can persist in forests, they are likely to grow more quickly and survive longer in dense stands, compared to savanna specialists. Furthermore, the faster growth rates of generalists may allow them to overtop and...

Registration Year

  • 2022

Resource Types

  • Dataset


  • North Carolina State University
  • University of Massachusetts Amherst
  • Virginia Tech
  • University of California, Riverside
  • Pennsylvania State University
  • Helmholtz Centre for Environmental Research
  • Center for Research and Advanced Studies of the National Polytechnic Institute
  • University of Pennsylvania
  • North Carolina Wildlife Resources Commission
  • Johann Heinrich von Thünen-Institut