The quantification of the biological diversity in environmental samples using high-throughput DNA sequencing is hindered by the PCR bias caused by variable primer–template mismatches of the individual species. In some dietary studies, there is the added problem that samples are enriched with predator DNA, so often a predator-specific blocking oligonucleotide is used to alleviate the problem. However, specific blocking oligonucleotides could coblock nontarget species to some degree. Here, we accurately estimate the extent of the...

Understanding how different biodiversity components are related across different environmental conditions is a major goal in macroecology and conservation biogeography. We investigated correlations among alpha and beta taxonomic (TD), phylogenetic (PD), and functional diversity (FD) in ant communities in the five biogeographic regions most representative of western Europe; we also examined the degree of niche conservatism. We combined data from 349 ant communities composed of 154 total species, which were characterized by 10 functional traits...

The complete raw dataset collected in the study
including the participants’ dataset.

Additional file 3: Table S3. GO Gene Ontology analysis of co-expressed protein-coding genes with all candidate lncRNAs.

Additional file 2: Additional Tables 1-5.

A document containing the attached files and their contents, as well as information on the software used for the analysis.

Supplemental material, sj-xlsx-4-tct-10.1177_15330338221124658 for In Vitro and in Vivo Study of the Effect of Osteogenic Pulsed Electromagnetic Fields on Breast and Lung Cancer Cells by Mike Y. Chen, Jing Li, Nianli Zhang and Erik I. Waldorff, James T. Ryaby, Philip Fedor, Yongsheng Jia, Yujun Wang in Technology in Cancer Research & Treatment

Supplemental material, sj-xlsx-4-tct-10.1177_15330338221124658 for In Vitro and in Vivo Study of the Effect of Osteogenic Pulsed Electromagnetic Fields on Breast and Lung Cancer Cells by Mike Y. Chen, Jing Li, Nianli Zhang and Erik I. Waldorff, James T. Ryaby, Philip Fedor, Yongsheng Jia, Yujun Wang in Technology in Cancer Research & Treatment

Additional file 4. Table S4. Characteristics of the 30 Spanish Farms included in this study.

Pollinators in agroecosystems are often exposed to pesticide mixtures. Even at low concentrations, the effects of these mixtures on bee populations are difficult to predict due to potential synergistic interactions. We orally exposed newly-emerged females of the solitary bee Osmia bicornis to environmentally-realistic levels of clothianidin (neonicotinoid insecticide) and propiconazole (fungicide), singly and in combination. The amount of feeding solution consumed was highest in bees exposed to the neonicotinoid, and lowest in bees exposed to...

Wang and Althoff (2019) explored the capacity of Drosophila melanogaster to exhibit adaptive plasticity in a novel environment. In a full-sib, half-sib design, they scored the activity of the enzyme alcohol dehydrogenase (ADH) and plastic responses, measured as changes in ADH activity across ethanol concentrations in the range of 0-10% (natural variation) and 16% (the novel environment). ADH activity increased with alcohol concentration, and there was a positive association between larval viability and ADH activity...

Larger geographical areas contain more species–an observation raised to a law in ecology. Less explored is whether biodiversity changes are accompanied by a modification of interaction networks. We use data from 32 spatial interaction networks from different ecosystems to analyse how network structure changes with area. We find that basic community structure descriptors (number of species, links, and links per species) increase with area following a power-law. Yet, the distribution of links per species varies...

Molecular dating methods of population splits are crucial in evolutionary biology, but they present important difficulties due to the complexity of the genealogical relationships of genes and past migrations between populations. Using the double digest restriction-site associated DNA (ddRAD) technique and an isolation-with-migration (IM) model, we studied the evolutionary history of water vole populations of the genus Arvicola, a group of complex evolution with fossorial and semi-aquatic ecotypes. To do this, we first estimated mutation...

Conservation requires both a needs assessment and prioritization scheme for planning and implementation. Range maps are critical for understanding and conserving biodiversity, but current range maps often omit content, negating important metrics of variation in populations and places. Here, we integrate a myriad of conditions that are spatially explicit across distributions of carnivores to identify gaps in capacity necessary for their conservation. Expanding on traditional gap analyses that focus almost exclusively on quantifying discordance in...

Additional file 10: Table S5. The summary of lncRNAs exhibiting allele-specific expression in cultivated sunflower lines for edible fruit and oil identified in 12 DAP sunflower endosperm.

Additional file 12: Table S7. GO Gene Ontology analysis of co-expressed protein-coding genes with lncRNAs of allelic bias toward cultivated lines for oil.

Additional file 2: Table S2. The genomic information of lncRNAs in sunflower endosperm at 12DAP.

Additional file 11: Table S6. GO Gene Ontology analysis of co-expressed protein-coding genes with lncRNAs of allelic bias toward cultivated lines for edible fruit.

Supplemental material, sj-xlsx-4-cep-10.1177_03331024221146317 for Genome-wide DNA methylation analysis in an antimigraine-treated preclinical model of cortical spreading depolarization by Marta Vila-Pueyo, Ester Cuenca-León, Ana C. Queirós, Marta Kulis, Cèlia Sintas, Bru Cormand, José Ignacio Martín-Subero, Patricia Pozo-Rosich, Noèlia Fernàndez-Castillo, Alfons Macaya in Cephalalgia

Supplemental material, sj-xlsx-5-cep-10.1177_03331024221146317 for Genome-wide DNA methylation analysis in an antimigraine-treated preclinical model of cortical spreading depolarization by Marta Vila-Pueyo, Ester Cuenca-León, Ana C. Queirós, Marta Kulis, Cèlia Sintas, Bru Cormand, José Ignacio Martín-Subero, Patricia Pozo-Rosich, Noèlia Fernàndez-Castillo, Alfons Macaya in Cephalalgia

Supplemental material, sj-xlsx-1-cep-10.1177_03331024221146317 for Genome-wide DNA methylation analysis in an antimigraine-treated preclinical model of cortical spreading depolarization by Marta Vila-Pueyo, Ester Cuenca-León, Ana C. Queirós, Marta Kulis, Cèlia Sintas, Bru Cormand, José Ignacio Martín-Subero, Patricia Pozo-Rosich, Noèlia Fernàndez-Castillo, Alfons Macaya in Cephalalgia

This study was conducted at the ForHot research site in Iceland (Sigurdsson et al., 2016) between August 2017 and June 2018 (64°0′N, 21°11′W). Soil type was a Brown Andosol (Arnalds, 2015). Mean annual temperature at the site was 5.1 °C. The coldest and warmest temperatures in the neighboring village of Eyrarbakki in 2016 were -12.3 °C and 21.6 °C, respectively. Average annual precipitation for the same year was 1153 mm (Icelandic Meteorological Office, 2016). The...

Supplemental material, sj-xlsx-3-tct-10.1177_15330338231160393 for Real-world Efficacy Data on Anti-Angiogenic Drugs in Recurrent Small Cell Cervical Carcinoma: A Retrospective Study by Haifeng Qiu, Ning Su, Shuping Yan and Jing Li in Technology in Cancer Research & Treatment

Supplemental material, sj-xlsx-1-tct-10.1177_15330338231160393 for Real-world Efficacy Data on Anti-Angiogenic Drugs in Recurrent Small Cell Cervical Carcinoma: A Retrospective Study by Haifeng Qiu, Ning Su, Shuping Yan and Jing Li in Technology in Cancer Research & Treatment

Additional file 2. Table S2. Characteristics of the 27 German Farms included in this study.