AFLP binary data

Nexus file of genetic data

Here we present a protocol to genetically detect diatoms in sediments of the Kenyan tropical Lake Naivasha, based on taxon-specific PCR amplification of short fragments (approx. 100 bp) of the small subunit ribosomal (SSU) gene and subsequent separation of species-specific PCR products by PCR-based DHPLC. An evaluation of amplicons differing in primer specificity to diatoms and length of the fragments amplified demonstrated that the number of different diatom sequence types detected after cloning of the...

This table includes results from cloning and direct sequencing after DHPLC collection, along with the Genbank accession numbers of the retrieved sequences. Reference is made to Figure 3 in the paper.

Description of haplotype sequences and haplotype composition of each sampled population

break-down of haplotype composition of each population, in arlequin format

The Selection, Constraint, Restraint, and Senescence Hypotheses predict how breeding success should vary with age. The Selection Hypothesis predicts between-individual variation arising from quality differences; the other hypotheses predict within-individual variation due to differing skills or physiological condition (Constraint), residual reproductive lifespan (Restraint), or somatic and reproductive investment (Senescence). Studies tend to focus on either the initial increase in breeding success or later decrease; however, both require consideration when unravelling the underlying evolutionary processes. Additionally,...

Supplementary Table 2: Mitochondrial sequence alignment showing variable sites. Supplementary Table 3: Synopsis alignment of Felis silvestris phylogenetically informative mitochondrial sites. Supplementary Table 4: Primer sequences for mitochondrial amplification of F. silvestris ssp. informative sites.

The use of scat surveys to obtain DNA has been well documented in temperate areas, where DNA preservation may be more effective than in tropical forests. Samples obtained in the tropics are often exposed to high humidity, warm temperatures, frequent rain, and intense sunlight, all of which can rapidly degrade DNA. Despite these potential problems, we demonstrate successful DNA amplification and sequencing for faeces of carnivores collected in tropical conditions and quantify how sample condition...

Tetraploid genotypes for the locus Aox27

White sturgeon (Acipenser transmontanus) in the Fraser River are listed as imperiled (the second highest possible rating) by the British Columbia Conservation Data Centre. A difficulty in trying to protect this species in the Fraser River and elsewhere is the lack of knowledge regarding their population biology. Variation in the mitochondrial DNA control region and at four microsatellite loci was examined in order to characterize white sturgeon samples from throughout the Fraser River mainstem and...

Data used to generate Figure 3 and related statistics. Data collected from the wild and managed with JMP V.7

DNA sequence studies frequently reveal evidence of cryptic lineages in morphologically uniform species, many of which turn out to be evolutionarily distinct species. The Common Raven (Corvus corax) includes two deeply divergent mtDNA lineages: one lineage seems restricted to western North America and the other is Holarctic in distribution. These deep clades hint of the possibility of cryptic species in the western United States. We tested this hypothesis in a population consisting of an equal...

The conditions of phenotypic and genetic population differentiation allow inferences about the evolution, preservation and loss of biological diversity. In Lake Tanganyika, water level fluctuations are assumed to have had a major impact on the evolution of stenotopic littoral species, though this hypothesis has not been specifically examined so far. The present study investigates whether subtly differentiated color patterns of adjacent Tropheus moorii populations are maintained in isolation or in the face of continuous gene...

The developmental differences between marsupials, placentals and monotremes are thought to be reflected in differing patterns of postcranial development and diversity. However, developmental polarities remain obscured by the rarity of monotreme data. Here I present the first postcranial ossification sequences of the monotreme echidna and platypus, and compare these with published data from other mammals and amniotes. Strikingly, monotreme stylopodia (humerus, femur) ossify after the more distal zeugopodia (radius/ulna, tibia/fibula), resembling only the European mole...

Cranial and dental data from 35 species of wild felids.

Archive of sequence electropherograms and Sequencher file with examples of genotyping HapSTR loci.

Shotgun data (in FASTA format) from Roche 454 titanium GS-FLX machine

Shotgun data (in FASTA format) from Roche 454 titanium GS-FLX machine

Single nucleotide polymorphism (SNP) genotype data in CONVERT format. Includes all loci in raw format.

Shotgun data (in FASTA format) from Roche 454 titanium GS-FLX machine

Shotgun data (in FASTA format) from Roche 454 titanium GS-FLX machine

Shotgun data (in FASTA format) from Roche 454 titanium GS-FLX machine

Shotgun data (in FASTA format) from Roche 454 titanium GS-FLX machine