2,197,024 Works

Supplementary figure 2. Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function

, Xian Yunxuan, , Yuriko Kobayashi, , Archana Panche & Hiroyuki Koyama
Supplementary Figure 1

Supplementary figure 2. Supplementary material for Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions

Pranvera Hiseni, Robert C Wilson, Ola Storrø, Torbjørn Øien, Knut Rudi & Roar Johnsen
Supplementary figure 2

Supplementary figure S3. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu &
Figure S3. As-synthesized GO (left) and rGO (right) solutions.

Supplementary figure S2. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu &
Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Supplementary figure S2. Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy

Zhi Li, Yael Zilberman, Qing-Bin Lu &
Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Supplementary Figure 1. Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach

Yuko Takagi, Hirokazu Nosato, , Koji Furukawa & Hidenori Sakanashi
Supplemental Figure1.T. cruzi epimastigote cell count estimation by conventional methods. (A)TC20automated cell counter(Bio-Rad, Hercules, California, USA)was used to count rosette-rich epimastigote, according to the manufacturer’s instruction. Actual cell count was determined by using hemocytometer after breaking apart cell aggregates by pipetting and vortexing. Dottedlinerepresentsthetheoreticallineofperfectcorrelationbetweenactual cell counts and machine-estimated cell counts. (B)Fiji-ImageJ software[1] was used to count the same sample as(A),using threshold adjustment and watershed segmentation functions.(C)Cell Profiler Worm Toolbox[2] was used to count the same...

Supplementary figure 2. Conducting polymer-based multilayer films for instructive biomaterial coatings

John G Hardy, , Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S2 - Scatter plot correlating the relationship between the cell orientation (angle relative to the dipping direction of the multilayer films) and length of the cells on the polymer films without and with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based films. Black circles represent cells without electrical stimulation, whereas red circles represent cells with electrical stimulation; black and red lines represent the corresponding trend lines.

Supplementary figure 3. Conducting polymer-based multilayer films for instructive biomaterial coatings

, John G Hardy, , Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S3 HUMAN MESENCHYMAL STEM CELL ADHESION STUDIES HMSCs were supplied by Lonza (Walkersville, MD). Samples were prepared as described above. After sterilization, the samples were incubated for 30 minutes in 24 well plates containing HMSC growth medium that was composed of: high glucose Dulbecco’s Modified Eagle Medium (DMEM, 440 mL); fetal bovine serum (50 mL); antibiotic-antimycotic (5 mL); non-essential amino acids (5 mL), and 2 ng mL-1 basic fibroblast growth factor. Medium was...

Supplementary figure 1. Conducting polymer-based multilayer films for instructive biomaterial coatings

John G Hardy, , Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S1 - Experimental setup for dip coating multilayer films using a Gilson 223 Sample Changer converted for use as a dip coater.

Supplementary figure 2. Conducting polymer-based multilayer films for instructive biomaterial coatings

John G Hardy, , Jacqueline K Chow, Sydney A Geissler, Austin B McElroy, Lindsey Nguy, Derek S Hernandez & Christine E Schmidt
Supplementary Figure S2 - Scatter plot correlating the relationship between the cell orientation (angle relative to the dipping direction of the multilayer films) and length of the cells on the polymer films without and with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based films. Black circles represent cells without electrical stimulation, whereas red circles represent cells with electrical stimulation; black and red lines represent the corresponding trend lines.

Supplementary figure S4. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S4. Agarose gel stained with Sybr Gold with single samples of 10, 20, 30 and 40 hour dialysis of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S3.

Figure S3 Supplementary material for ‘A simple dialysis device for large DNA molecules’

, Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane–polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Consequently, we describe empirical rates of dialysis,...

Supplementary figure S3. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S3. Agarose gel stained with Sybr Gold with multiple 20 and 40 hour dialysis samples of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S2.

Supplementary figure S3. A simple dialysis device for large DNA molecules

Samuel JW Krerowicz, Juan P Hernandez-Ortiz & David C Schwartz
Supplementary Figure S3. Agarose gel stained with Sybr Gold with multiple 20 and 40 hour dialysis samples of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S2.

Supplementary Figure S1. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures

Juliette Ohan, Benjamin Pellea, , , Blake Hovde, , Armand E.K.Dichosa & Shawn R. Starkenburg
Pre-cultivation targeted FACS enrichment of aGMDs and abGMDs. (A) Only aGMDs and abGMDs containing algal/bacterial cells were first sorted by size by measuring forward scatter (FSC, x-axis at linear scale) versus side scatter (SSC, y-axis at logarithmic scale). (B) aGMDs and abGMDs were further analyzed by chlorophyll autofluorescence using a 650/50 nm filter (x-axis at logarithmic scale) versus 530/40 nm filter (y-axis at logarithmic scale), to select for GMDs containing algal/bacterial cells. (C) aGMDs and...

Supplementary Figure S3. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures

Juliette Ohan, Benjamin Pellea, , , Blake Hovde, , Armand E.K.Dichosa & Shawn R. Starkenburg
Post-cultivation targeted FACS enrichment of abGMDs. Control population of cultivated aGMDs (A) and experimental cultivated population of abGMDs (D) using FSC (x-axis; linear scale) versus SSC (y-axis; log scale). (E) Cultivated abGMDs exhibiting chlorophyll autofluorescence higher than that of the cultivated control aGMDs shown in (B) using a 650/50 nm filter (x-axis at logarithmic scale) versus 530/40 nm filter (y-axis at logarithmic scale). (F) Gated selection of cultivated abGMDs exhibiting higher FSC (to distinguish from...

Supplementary Figure S2. High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures

Juliette Ohan, Benjamin Pellea, , , Blake Hovde, , Armand E.K.Dichosa & Shawn R. Starkenburg
Schematic for cultivation chamber. The cultivation chamber was fabricated and sterilized as described for the microfluidic droplet generator chip (Figure 2 legend). The microfluidic cultivation chamber was used to microscopically visualize droplets without disturbing them or sacrificing viability, as well as providing an even distribution of light.

Supplementary figure 2. A mismatch-tolerant RT-quantitative PCR: application to broad-spectrum detection of respiratory syncytial virus

, , , Yi Zhou, Qin Chen &
Maximum likelihood (ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B sequences obtained from four clinical samples (Sp16, Sp19, Sp8 and Sp9) in this study. The reference sequences were retrieved from GenBank.

Supplementary figure 1. Fluidic resistance control enables high throughput establishment of mixed species biofilms

Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder & Mette Burmølle
Viability is unaffected by short time incubation in high viscosity medium. (A) The number of counted CFU/ml of Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the viability of Xanthomonas retroflexus was unaffected by incubation in high viscosity liquid and did not change over a short time period (N=3, Error bars represent Standard Error (SE)).

Supplementary Figure 1: Staining kinetics of PI and Hoechst in 3D organoids.

Stefanie Mueller, Konstantin J. Bode, Matthias Schweinlin, Marco Metzger & Thomas Brunner
Intact (A) and damaged (B) organoids were stained with PI&Hoechst. Subsequently, brightfield (BF) and fluorescence microscopic images were taken 5, 10 ,15 ,30, and 60 minutes after the dyes were added to culturing medium. (scale bar = 100 µm)

Predictive value and clinical significance of increased SSAT-1 activity in healthy adults: graphical abstract

Paramjit S Tappia, Andrew W Maksymiuk, Daniel S Sitar, Parveen S Akhtar, , , , Rashid Bux Ahmed, Brian Cheng, Gina Huang, Horacio Bach, Brett Hiebert & Bram Ramjiawan
Graphical Abstract. Aim: Spermidine/spermine N1-acetyltransferase (SSAT-1) regulates cell growth, proliferation and death. Amantadine is converted by SSAT-1 to acetylamantadine (AA). Although SSAT-1 is activated in patients with cancer, a number of ostensibly healthy adult volunteers had higher than expected AA concentration. This study was undertaken to examine the outlier group. Results: In some of the outlier controls, higher than expected AA concentration was linked to increased serum carcinoembryonic antigen. Clinical and radiographic assessments revealed underlying...

Supplementary Figure 1. Sustained suppression of IL-18 by employing a vaccine ameliorates intestinal inflammation in TNBS-induced murine colitis

, Richard Warrington, , , Carolyn Weiss &
The position of human corresponding vaccine A and D peptides in human IL-18. Green represents Vaccine A peptide; Brown represents Vaccine D peptide.

Supplementary Figures S1 & S2, and Tables S1-S5 for: Improvement of multiplex semi-nested PCR system for screening rare mutations by High-throughput sequencing

, Xue Chi, , &
Supplementary Figure S1. Improvement of PCR reaction system using dilution comparing to spin column. M: 100bp marker. (A) The patterns of the first/second-round PCR reaction system by agarose gel electrophoresis. For the 50μL PCR reaction system, compared with the previously reported primer concentration which was 3.3μL (ITSP1s) and 0.66μL (AP1) in Lane l, Lane 2 shows no difference in the yield when primer concentration was 2.2μL (ITSP1s) and0.44μL (AP1). For the 10μL PCR reaction system,...

Supplemental Figure 1. Bright-field image of B. cereus cells taken from a stationary phase culture and incubated in a 0.4% EB solution. In two instances EB specifically stains a single compartment in a filamentous chain.

Josef D. Franke, Ann L. Braverman, Alison M. Cunningham, Erin E. Eberhard & Greg A Perry
Supplemental Figure 1. Bright-field image of B. cereus cells taken from a stationary phase culture and incubated in a 0.4% EB solution. In two instances EB specifically stains a single compartment in a filamentous chain.

Supplementary figure 1. The standard curve graph of HPV18 reference plasmid by CerviHPV assay. In addition to HPV18 amplification signal, unexpected amplification signal appeared in 12HPV channel.

, , , , , Yong Ma &
Supplementary Figure 1. The standard curve graph of HPV18 reference plasmid by CerviHPV assay. In addition to HPV18 amplification signal, unexpected amplification signal appeared in 12HPV channel.

Registration Year

  • 2020
    333,766
  • 2019
    761,017
  • 2018
    130,399
  • 2017
    175,694
  • 2016
    328,994
  • 2015
    43,888
  • 2014
    62,030
  • 2013
    107,602
  • 2012
    87,161
  • 2011
    13,926

Resource Types

  • Image
    2,197,024

Affiliations

  • University of Pittsburgh
    20
  • University of Luxembourg
    6
  • Technical University of Crete
    5
  • Drexel University
    4
  • RIKEN
    2
  • Institute for Research in Biomedicine
    2
  • Trinity College Dublin
    2
  • Memorial Sloan Kettering Cancer Center
    2
  • Institute of Biomedical Research of Barcelona
    2
  • Institució Catalana de Recerca i Estudis Avançats
    2