Supplementary Figure 1

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Figure S3. As-synthesized GO (left) and rGO (right) solutions.

Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Figure S2. The effect of ssDNA-Au electrode treated with TMPD only.Figure S2 shows the CV curves of [Fe(CN)6]3−/[Fe(CN)6]4− recorded using ssDNA-Au electrode after treatment with 1 mM TMPD only at various time points. The nearly identical current responses over time showed that TMPD itself has little effect on [Fe(CN)6]3−/[Fe(CN)6]4− signal recovery.

Supplemental Figure1.T. cruzi epimastigote cell count estimation by conventional methods. (A)TC20automated cell counter(Bio-Rad, Hercules, California, USA)was used to count rosette-rich epimastigote, according to the manufacturer’s instruction. Actual cell count was determined by using hemocytometer after breaking apart cell aggregates by pipetting and vortexing. Dottedlinerepresentsthetheoreticallineofperfectcorrelationbetweenactual cell counts and machine-estimated cell counts. (B)Fiji-ImageJ software[1] was used to count the same sample as(A),using threshold adjustment and watershed segmentation functions.(C)Cell Profiler Worm Toolbox[2] was used to count the same...

Supplementary Figure S2 - Scatter plot correlating the relationship between the cell orientation (angle relative to the dipping direction of the multilayer films) and length of the cells on the polymer films without and with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based films. Black circles represent cells without electrical stimulation, whereas red circles represent cells with electrical stimulation; black and red lines represent the corresponding trend lines.

Supplementary Figure S3 HUMAN MESENCHYMAL STEM CELL ADHESION STUDIES HMSCs were supplied by Lonza (Walkersville, MD). Samples were prepared as described above. After sterilization, the samples were incubated for 30 minutes in 24 well plates containing HMSC growth medium that was composed of: high glucose Dulbecco’s Modified Eagle Medium (DMEM, 440 mL); fetal bovine serum (50 mL); antibiotic-antimycotic (5 mL); non-essential amino acids (5 mL), and 2 ng mL-1 basic fibroblast growth factor. Medium was...

Supplementary Figure S1 - Experimental setup for dip coating multilayer films using a Gilson 223 Sample Changer converted for use as a dip coater.

Supplementary Figure S2 - Scatter plot correlating the relationship between the cell orientation (angle relative to the dipping direction of the multilayer films) and length of the cells on the polymer films without and with electrical stimulation. A) PEDOT-PSS-based films. B) PEDOT-S-based films. Black circles represent cells without electrical stimulation, whereas red circles represent cells with electrical stimulation; black and red lines represent the corresponding trend lines.

Supplementary Figure S4. Agarose gel stained with Sybr Gold with single samples of 10, 20, 30 and 40 hour dialysis of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S3.

The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane–polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Consequently, we describe empirical rates of dialysis,...

Supplementary Figure S3. Agarose gel stained with Sybr Gold with multiple 20 and 40 hour dialysis samples of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S2.

Supplementary Figure S3. Agarose gel stained with Sybr Gold with multiple 20 and 40 hour dialysis samples of a mixture of  + 1 kb+ DNA sizing ladder using 0.4 um pore membranes. The relative and absolute quantitations of each band can be found in Supplementary Table S2.

Pre-cultivation targeted FACS enrichment of aGMDs and abGMDs. (A) Only aGMDs and abGMDs containing algal/bacterial cells were first sorted by size by measuring forward scatter (FSC, x-axis at linear scale) versus side scatter (SSC, y-axis at logarithmic scale). (B) aGMDs and abGMDs were further analyzed by chlorophyll autofluorescence using a 650/50 nm filter (x-axis at logarithmic scale) versus 530/40 nm filter (y-axis at logarithmic scale), to select for GMDs containing algal/bacterial cells. (C) aGMDs and...

Post-cultivation targeted FACS enrichment of abGMDs. Control population of cultivated aGMDs (A) and experimental cultivated population of abGMDs (D) using FSC (x-axis; linear scale) versus SSC (y-axis; log scale). (E) Cultivated abGMDs exhibiting chlorophyll autofluorescence higher than that of the cultivated control aGMDs shown in (B) using a 650/50 nm filter (x-axis at logarithmic scale) versus 530/40 nm filter (y-axis at logarithmic scale). (F) Gated selection of cultivated abGMDs exhibiting higher FSC (to distinguish from...

Schematic for cultivation chamber. The cultivation chamber was fabricated and sterilized as described for the microfluidic droplet generator chip (Figure 2 legend). The microfluidic cultivation chamber was used to microscopically visualize droplets without disturbing them or sacrificing viability, as well as providing an even distribution of light.

Maximum likelihood (ML) tree of sequences from four clinical samples. ML tree of RSV-A and RSV-B sequences obtained from four clinical samples (Sp16, Sp19, Sp8 and Sp9) in this study. The reference sequences were retrieved from GenBank.

Viability is unaffected by short time incubation in high viscosity medium. (A) The number of counted CFU/ml of Pseudomonas putida and Paenibacillus amylolyticus is identical after 45 min. of incubation in PBS and high viscosity liquid (50% glycerol). (B) Similarly, the viability of Xanthomonas retroflexus was unaffected by incubation in high viscosity liquid and did not change over a short time period (N=3, Error bars represent Standard Error (SE)).

Intact (A) and damaged (B) organoids were stained with PI&Hoechst. Subsequently, brightfield (BF) and fluorescence microscopic images were taken 5, 10 ,15 ,30, and 60 minutes after the dyes were added to culturing medium. (scale bar = 100 µm)

Graphical Abstract. Aim: Spermidine/spermine N1-acetyltransferase (SSAT-1) regulates cell growth, proliferation and death. Amantadine is converted by SSAT-1 to acetylamantadine (AA). Although SSAT-1 is activated in patients with cancer, a number of ostensibly healthy adult volunteers had higher than expected AA concentration. This study was undertaken to examine the outlier group. Results: In some of the outlier controls, higher than expected AA concentration was linked to increased serum carcinoembryonic antigen. Clinical and radiographic assessments revealed underlying...

The position of human corresponding vaccine A and D peptides in human IL-18. Green represents Vaccine A peptide; Brown represents Vaccine D peptide.

Supplementary Figure S1. Improvement of PCR reaction system using dilution comparing to spin column. M: 100bp marker. (A) The patterns of the first/second-round PCR reaction system by agarose gel electrophoresis. For the 50μL PCR reaction system, compared with the previously reported primer concentration which was 3.3μL (ITSP1s) and 0.66μL (AP1) in Lane l, Lane 2 shows no difference in the yield when primer concentration was 2.2μL (ITSP1s) and0.44μL (AP1). For the 10μL PCR reaction system,...

Supplemental Figure 1. Bright-field image of B. cereus cells taken from a stationary phase culture and incubated in a 0.4% EB solution. In two instances EB specifically stains a single compartment in a filamentous chain.

Supplementary Figure 1. The standard curve graph of HPV18 reference plasmid by CerviHPV assay. In addition to HPV18 amplification signal, unexpected amplification signal appeared in 12HPV channel.