Si estas visitando formosa y quieres disfrutar la ciudad junto a tu familia a un bajo precio y con un coche muy cómodo puedes dirigirte a nuestras oficinas de Alquiler de autos en Formosa donde te atenderemos muy amablemente para ofrecerte muy buenos coches a excelentes precios, y si no tienes mucho tiempo puedes hacerte una idea de nuestros precios a través de la pagina web que disponemos

Si estas visitando formosa y quieres disfrutar la ciudad junto a tu familia a un bajo precio y con un coche muy cómodo puedes dirigirte a nuestras oficinas de Alquiler de autos en Formosa donde te atenderemos muy amablemente para ofrecerte muy buenos coches a excelentes precios, y si no tienes mucho tiempo puedes hacerte una idea de nuestros precios a través de la pagina web que disponemos

Model-to-data paradigm enables researchers to train and evaluate models on critical data derived from
health care and clinical trials.

Model-to-data paradigm enables researchers to train and evaluate models on critical data derived from health care and clinical trials.

Model-to-data paradigm enables researchers to train and evaluate models on critical data derived from health care and clinical trials.

Supplemental Fig. 3. Effect of diet and genotype in body weight at the premalignant lesion and colon mass formation cohorts. At the premalignant lesion cohort, A: Weekly body weight (in grams) is not significantly different between genotype (WT or AhrΔIEC) or diet (Low-fat diet, LFD or High-fat diet, HFD). B and C: At termination (week 29), no differences in body weight can be observed between genotype (p=0.4463) and diet (p=0.2260). At the colon mass formation...

Supplemental Fig. 6. A. Representative images of immunofluorescence stained proliferative cells (EdU in green) and nuclei (DAPI in blue) within colon masses of both WT and AhrΔIEC mice. B. Representative images of immunofluorescence stained 𝛽-catenin (in red) and nuclei (DAPI in blue) within colon masses of both LFD and HtLFD fed WT and AhrΔIEC mice. Magnified images (yellow box) contain representative regions of interest (ROIs) outlined in yellow that were used to calculate 𝛽-catenin intensity...

Supplemental Fig. 1. Model verification in the AhrΔIEC mice. A: Schematic representation of Cre-lox intestinal-specific knockout (AhrΔIEC) and controls (AhRf/f) used in the study. B: AhR mRNA expression normalized to 18s rRNA. AhRf/f (f/f, WT) vs Ahrf/fxVillinCre (f/f Cre, AhrΔIEC) from isolated colonic crypts (n= 6). C: Representative Western blot analysis of AhR protein expression. β-actin was used as a loading control. *p<0.05 vs. control (WT). Values are means ± SEM. * indicates p ≤...

Model verification in the AhrΔIEC mice. A: Schematic representation of Cre-lox intestinal-specific knockout (AhrΔIEC) and controls (AhRf/f) used in the study. B: AhR mRNA expression normalized to 18s. AhRf/f (f/f) vs Ahrf/fxVCre (f/f Cre) from isolated colonic crypts (mean ± SEM, n= 6). C: Representative Western blot analysis of AhR protein expression. β-actin was used as a loading control. Values are means ± SEM. *p < 0.05 vs. control (WT).

Supplemental Fig. 1. Model verification in the AhrΔIEC mice. A: Schematic representation of Cre-lox intestinal-specific knockout (AhrΔIEC) and controls (AhRf/f) used in the study. B: AhR mRNA expression normalized to 18s rRNA. AhRf/f (f/f, WT) vs Ahrf/fxVillinCre (f/f Cre, AhrΔIEC) from isolated colonic crypts (n= 6). C: Representative Western blot analysis of AhR protein expression. β-actin was used as a loading control. *p<0.05 vs. control (WT). Values are means ± SEM. * indicates p ≤...

Supplemental Fig. 4. Effect of genotype in cell proliferation in intact crypts at the proximal section of the colon at the ACF formation stage. A: Proliferative zone EdU+ cells calculated as 100 times the position of the highest labeled cell divided by their corresponding crypt length in mm, *p=0.03 vs. AhrΔIEC. B: Crypt length in mm by genotype, WT mean 146 mm, and AhrΔIEC mean 148 mm (p=0.2860 (MW). Values are means ± SEM. *...

Supplemental Fig. 4. Effect of genotype in cell proliferation in intact crypts at the proximal section of the colon at the ACF formation stage. A: Proliferative zone EdU+ cells calculated as 100 times the position of the highest labeled cell divided by their corresponding crypt length in mm, *p=0.03 vs. AhrΔIEC. B: Crypt length in mm by genotype, WT mean 146 mm, and AhrΔIEC mean 148 mm (p=0.2860 (MW). Values are means ± SEM. *...

Supplemental Fig. 6. A. Representative images of immunofluorescence stained proliferative cells (EdU in green) and nuclei (DAPI in blue) within colon masses of both WT and AhrΔIEC mice. B. Representative images of immunofluorescence stained 𝛽-catenin (in red) and nuclei (DAPI in blue) within colon masses of both LFD and HtLFD fed WT and AhrΔIEC mice. Magnified images (yellow box) contain representative regions of interest (ROIs) outlined in yellow that were used to calculate 𝛽-catenin intensity...

Supplemental Fig. 5. Ingenuity Pathway analysis of significant deferentially expressed genes (DEGs) upregulated in the absence of AhR in IEC. A. Top enriched categories of canonical pathways with a p-value less than 10-5 with positive z-scores. The x-axis represents the pathways identified. The y-axis shows the −log of the P value calculated based on Fisher’s exact test. Horizontal orange line running through the bars is the threshold for p value for these pathways' enrichment B....

Supplemental Fig. 3. Effect of diet and genotype in body weight at the premalignant lesion and colon mass formation cohorts. At the premalignant lesion cohort, A: Weekly body weight (in grams) is not significantly different between genotype (WT or AhrΔIEC) or diet (Low-fat diet, LFD or High-fat diet, HFD). B and C: At termination (week 29), no differences in body weight can be observed between genotype (p=0.4463) and diet (p=0.2260). At the colon mass formation...

Supplemental Fig. 5. Ingenuity Pathway analysis of significant deferentially expressed genes (DEGs) upregulated in the absence of AhR in IEC. A. Top enriched categories of canonical pathways with a p-value less than 10-5 with positive z-scores. The x-axis represents the pathways identified. The y-axis shows the −log of the P value calculated based on Fisher’s exact test. Horizontal orange line running through the bars is the threshold for p value for these pathways' enrichment B....

Supplemental Fig. 2. Individual observations of ACF/distal colon (cm) by genotype. Each mouse ACF#/cm is represented by individual columns with their respective data point above them. Dashed line represents the mean per genotype (x̅).

Supplemental Fig. 2. Individual observations of ACF/distal colon (cm) by genotype. Each mouse ACF#/cm is represented by individual columns with their respective data point above them. Dashed line represents the mean per genotype (x̅).

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