46 Works

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

National survey on unplanned readmissions for diabetic kidney disease: Insight from a new paradigm of obesity

Wei Zhang, Hang Dong, Yiping Cheng, Fang Zhong, Zinuo Yuan, Yingchun Dong, Rong Wang, Shumin Mu, Jiajun Zhao, Wenxia Han & Xiude Fan
Supplementary materials to the article ' National survey on unplanned readmissions for diabetic kidney disease: Insight from a new paradigm of obesity'

Additional file 6 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 6: Supplementary Figure 6.

Additional file 1 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 1: Figure sup 1. Opposing effects of apoE2 and apoE4 on Iba1 proteinlevels and their correlation with the amount of myelin debris in the corpuscallosum of apoE-TR mice upon cuprizone-induced demyelination. ApoE-TR micewere fed with either normal diet (CTL, n=5/genotype), or CPZ-containing diet(CPZ, n=5-6/genotype) for four weeks. (A,B) Iba1 protein level inthe CC region was assessed by Western blot (WB) analysis, and the fold change (CPZ vs. CTL) wasquantified. (C) A negative correlation...

Additional file 1 of Gastric duplication cyst with ectopic pancreas in a teenager successfully resected by endoscopic submucosal dissection

Xiaodan Ye, Muqing Wang, Yuanyuan Wang, Daiying Lin & Xiaozhong Wang
Additional file 1: Fig S1. The cystic portion’s cyst wall was lined by epithelium of gastric and surrounded by smooth muscle (hematoxylin and eosin [H&E] stain, ×10).

Additional file 1 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 1: Figure sup 1. Opposing effects of apoE2 and apoE4 on Iba1 proteinlevels and their correlation with the amount of myelin debris in the corpuscallosum of apoE-TR mice upon cuprizone-induced demyelination. ApoE-TR micewere fed with either normal diet (CTL, n=5/genotype), or CPZ-containing diet(CPZ, n=5-6/genotype) for four weeks. (A,B) Iba1 protein level inthe CC region was assessed by Western blot (WB) analysis, and the fold change (CPZ vs. CTL) wasquantified. (C) A negative correlation...

Additional file 6 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 6: Figure sup 6. The key molecules that regulate microglialfunctions are up-regulated in an apoE isoform-dependent manner uponcuprizone-induced demyelination. RNA was extracted from the CC area of CTL(n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice. (A-H) The expression of genes relatedto microglial activation, inflammation, and lipid sensing (Trem2, Tyrobp,Aim2, Btk, B2m, Ctsd, C3, and Cr3) was analyzed by real-time PCR. (I-L) The expression of genes involvedin microglial migration (Mmp2 and Mmp12) and phagocytosis (Axl)were examined...

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

Additional file 4 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 4: Figure sup 4. Transcriptional changes of selected astrocyte-enrichedgenes in apoE-TR mice upon cuprizone-induced demyelination. The CC region of apoE-TR mice treated with normal or CPZ diet was subjected to transcriptomic profiling. Heat map showing transcriptional changes (CPZ vs CTL groups) of selected astrocyte-enriched genes identified from RNA-Seq. The scaled expression value (row Z score) is shown with a blue-red color scheme, denoting red as higher expression, and blue as lower expression.

Additional file 3 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 3: Figure sup 3. Similar levels of astrogliosis were observed in apoE-TR mice upon cuprizone-induced demyelination. The astrogliosis in the CC area of CTL (n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice was examined by immunostaining for GFAP. (A) Representative images of GFAP+astrocytes in the CC area of apoE-TR mice are shown. Scale bar, 100 µm. (B) The morphology of GFAP+astrocytes in apoE-TR mice are shown. Scale bar, 25 µm. (C) The fold change of...

Additional file 2 of Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelination

Na Wang, Minghui Wang, Suren Jeevaratnam, Cassandra Rosenberg, Tadafumi C. Ikezu, Francis Shue, Sydney V. Doss, Alla Alnobani, Yuka A. Martens, Melissa Wren, Yan W. Asmann, Bin Zhang, Guojun Bu & Chia-Chen Liu
Additional file 2: Figure sup 2. Lack of effects of cuprizone-induceddemyelination on the blood-brainbarrier integrity among apoE-TR mice. (A) Brain samples ofapoE-TR mice from CPZ-treated group (n=16/genotype) were subjected toimmunofluorescence staining for CLDN5(a tight junction-associated protein) and Glut1 (vascular marker). Representative images of CLDN5 (Green) staining and Glut1 (Red)staining in the CC region of apoE-TR mice are shown. Scale bar, 50 µm. Values are mean ± SEM. One-way ANOVA.N.S. not significant.

Additional file 2 of CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis

Bo Yang, Bin Zhang, Qi Qi & Changli Wang
Additional file 2: Fig. S2. Validation of the expression of miRNA targets between NSCLC cells and HBE cells. (A) CircInteractome database ( https://circinteractome.nia.nih.gov/ ) revealed multiple potential miRNA targets of circ_0017109. (B) qRT-PCR analysis of miRNA expression between NSCLC cells and HBE cells. All data were normalized to the expression level in HBE cells. ***, P < 0.001.

Additional file 1 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 1: Supplementary Figure 1.

Additional file 2 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 2: Supplementary Figure 2.

Additional file 3 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 3: Supplementary Figure 3.

Additional file 2 of Gastric duplication cyst with ectopic pancreas in a teenager successfully resected by endoscopic submucosal dissection

Xiaodan Ye, Muqing Wang, Yuanyuan Wang, Daiying Lin & Xiaozhong Wang
Additional file 2: Fig S2. The solid portion was pancreatic tissues composed of acini, ducts, and islets of Langerhans (hematoxylin and eosin [H&E] stain, ×10).

Additional file 1 of CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis

Bo Yang, Bin Zhang, Qi Qi & Changli Wang
Additional file 1: Fig. S1. Circ_0017109 promotes cell growth and inhibits apoptosis of NSCLC cells. (A) Circ_0017109 expression within H1299 and A549 cells after the transfection of si-circ_0017109#2 or si-NC. (B) CCK-8 assay in H1299 and A549 cells after the transfection of si-circ_0017109#2 or si-NC. (C) Apoptosis detection in H1299 and A549 cells after the transfection of si-circ_0017109 or si-NC. **, P < 0.01, and ***, P < 0.001.

Additional file 2 of CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis

Bo Yang, Bin Zhang, Qi Qi & Changli Wang
Additional file 2: Fig. S2. Validation of the expression of miRNA targets between NSCLC cells and HBE cells. (A) CircInteractome database ( https://circinteractome.nia.nih.gov/ ) revealed multiple potential miRNA targets of circ_0017109. (B) qRT-PCR analysis of miRNA expression between NSCLC cells and HBE cells. All data were normalized to the expression level in HBE cells. ***, P < 0.001.

Additional file 3 of CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis

Bo Yang, Bin Zhang, Qi Qi & Changli Wang
Additional file 3: Fig. S3. qRT-PCR analysis of FZD2, FZD3, FZD4 and FZD7 in NSCLC cells and HBE cells. All data were normalized to the expression level in HBE cells. *, P < 0.05, **, P < 0.01***, P < 0.001.

National survey on unplanned readmissions for diabetic kidney disease: Insight from a new paradigm of obesity

Wei Zhang, Hang Dong, Yiping Cheng, Fang Zhong, Zinuo Yuan, Yingchun Dong, Rong Wang, Shumin Mu, Jiajun Zhao, Wenxia Han & Xiude Fan
Supplementary materials to the article ' National survey on unplanned readmissions for diabetic kidney disease: Insight from a new paradigm of obesity'

Additional file 3 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 3: Supplementary Figure 3.

Additional file 4 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 4: Supplementary Figure 4.

Additional file 5 of Identification of tumor-associated antigens and immune subtypes of lower-grade glioma and glioblastoma for mRNA vaccine development

Zhi-liang Wang, Ruo-yu Huang, Bo Han, Fan Wu, Zhi-yan Sun, Guan-zhang Li, Wei Zhang, Zheng Zhao & Xing Liu
Additional file 5: Supplementary Figure 5.

Additional file 1 of Effects of mitochondrial dysfunction on bone metabolism and related diseases: a scientometric study from 2003 to 2022

Wei Zhang, Chang-Liang Xia, Jun-Nan Ma, Jia-Xuan Li, Qi Chen, Shuan-Ji Ou, Yang Yang, Yong Qi & Chang-Peng Xu
Additional file 1: SupplementaryFigure 1. The top 10 cited countrys related to this field.

Additional file 2 of Effects of mitochondrial dysfunction on bone metabolism and related diseases: a scientometric study from 2003 to 2022

Wei Zhang, Chang-Liang Xia, Jun-Nan Ma, Jia-Xuan Li, Qi Chen, Shuan-Ji Ou, Yang Yang, Yong Qi & Chang-Peng Xu
Additional file 2: SupplementaryFigure 2. The network map of top 10 institutions (A) and the top 10 cited networkmap of institutions in this field (B).

Registration Year

  • 2022
    44
  • 2020
    2

Resource Types

  • Image
    46

Affiliations

  • Northwestern University
    46
  • Sun Yat-sen University
    46
  • Nanjing Medical University
    44
  • Huazhong University of Science and Technology
    42
  • Zhejiang University
    40
  • University of Electronic Science and Technology of China
    40
  • First Affiliated Hospital of Soochow University
    40
  • First Affiliated Hospital of Jinan University
    38
  • Fudan University
    30
  • Sichuan University
    26