86 Works

Additional file 1 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 1 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 2 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 2: Figure S2. Schematic representation of the sequences of the dual luciferase reporter gene vectors for the 4 miRNAs.

Additional file 4 of Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Yanchun Li, Bing Xu, Xueying Ren, Luyang Wang, Yaqing Xu, Yefeng Zhao, Chen Yang, Chen Yuan, Huanjuan Li, Xiangmin Tong, Ying Wang & Jing Du
Additional file 4: Figure S4. (A) Analysis of NQO1 expression by RT-PCR in CISD2 silenced HT-1080 cells with or without the treatment of erastin; (B) Quantitative analysis of NQO1 expression in Fig. 4A;★P < 0.05 and ★★P < 0.01 between the indicated groups.

Additional file 1 of Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles

Kohei Yuyama, Hui Sun, Yasuyuki Igarashi, Kenji Monde, Takumi Hirase, Masato Nakayama & Yoichi Makino
Additional file 1: Supplemental Fig. 1. (A) Representative image of N2a-derived EVs using electron microscopy. (B) Size distribution of N2a-derived EVs analyzed by a nanoparticle analyzer, qNano.

Additional file 2 of Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles

Kohei Yuyama, Hui Sun, Yasuyuki Igarashi, Kenji Monde, Takumi Hirase, Masato Nakayama & Yoichi Makino
Additional file 2: Supplemental Fig. 2. Uncropped membrane images of western blot analysis. (A) N2a cells and N2a-derived EVs. (B) APP-N2a cells and APP-N2a cells-derived EVs.

Additional file 1 of CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma

Jungang Zhao, Rizhao Li, Jiacheng Li, Ziyan Chen, Zixia Lin, Baofu Zhang, Liming Deng, Gang Chen & Yi Wang
Supplementary Material 1

Additional file 2 of CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma

Jungang Zhao, Rizhao Li, Jiacheng Li, Ziyan Chen, Zixia Lin, Baofu Zhang, Liming Deng, Gang Chen & Yi Wang
Supplementary Material 2

Additional file 3 of CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma

Jungang Zhao, Rizhao Li, Jiacheng Li, Ziyan Chen, Zixia Lin, Baofu Zhang, Liming Deng, Gang Chen & Yi Wang
Supplementary Material 3

Additional file 4 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 4: Figure S4. The responses of LPS-treated cultured Schwann cells. A-E. mRNA expression levels of the inflammatory factors, IL-1β, IL-6, TNF-α, and TGF-β (A-D), the apoptosis marker, caspase 3 (E) and in LPS-treated Schwann cells (0, 100, 500, and 1000 ng/ml, 24 h at 37℃). F. Cytomorphology of LPS-treated Schwann cells at different doses (0, 100, 500, and 1000 ng/ml) under a light microscope. All data are mean ± SEM. n = 3....

Additional file 7 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 7: Figure S7. Cytokine array reveals Panx 1-dependent cytokines and chemokines in LPS-treated Schwann cells. A. Coordinates of the cytokine array containing 40 different cytokines and chemokines, including three positive control (PC) proteins. B. Array membranes of protein expression among Ctrl, Ctrl-siRNA, and Panx 1-siRNA groups in LPS-treated Schwann cells.

Additional file 3 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 3: Supplementary Figure S3. The survival curves of necroptosis-score. A-D Kaplan–Meier curve was used to analyze the survival rate of CESC (A), OV (B), UCEC (C), and UCS (D) patients with high or low necroptosis-score. Black curves indicate low necroptosis-score and red curves indicate high necroptosis-score.

Additional file 4 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 4: Supplementary Figure S4. Statistical analysis of the number of differential NRGs and prognostic NRGs of the four GCs. Red bars show up-regulated NRGs in cancer tissue. The green bars show NRGs that are downregulated in cancer tissue. The blue bars show the sum of the differential NRGs. Yellow bars indicate prognostic NRGs.

Additional file 8 of Downregulation of zinc finger protein 71 in laryngeal squamous cell carcinoma tissues and its potential molecular mechanism and clinical significance: a study based on immunohistochemistry staining and data mining

Fang-Cheng Jiang, Jia-Yuan Luo, Yi-Wu Dang, Hui-Ping Lu, Dong-Ming Li, Zhi-Guang Huang, Yu-Lu Tang, Ye-Ying Fang, Yu-Xing Tang, Ya-Si Su, Wen-Bin Dai, Shang-Ling Pan, Zhen-Bo Feng, Gang Chen & Juan He
Additional file 8: Figure S3. Expression analysis of ZNF71’s putative target and tight junctions-related genes.

Additional file 2 of Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor

Can Cui, Dengwen Zhang, Ke Sun, Yi Zhu, Jindong Xu, Yin Kang, Guangyan Zhang, Yujin Cai, Songsong Mao, Ruichun Long, Jue Ma, Song Dong & Yi Sun
Additional file 1: Figure S2. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S2 is related to Fig. 5A.

Additional file 3 of Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor

Can Cui, Dengwen Zhang, Ke Sun, Yi Zhu, Jindong Xu, Yin Kang, Guangyan Zhang, Yujin Cai, Songsong Mao, Ruichun Long, Jue Ma, Song Dong & Yi Sun
Additional file 1: Figure S3. Propofol inhibited invasion and migration of lung cancer cells. A Effect of different doses (0.3 μg/mL, 3 μg/mL, 30 μg/mL) of propofol on lung cancer cell viability was measured by CCK-8 assay; B Cell invasion were detected using Transwell assay; C Cell migration was detected Transwell assay; D Positive rate of E-cadherin protein was detected by immunocytochemistry and the stronger red fluorescence meant the higher positive rate. All experiments were...

Additional file 2 of Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Yanchun Li, Bing Xu, Xueying Ren, Luyang Wang, Yaqing Xu, Yefeng Zhao, Chen Yang, Chen Yuan, Huanjuan Li, Xiangmin Tong, Ying Wang & Jing Du
Additional file 2: Figure S2. CISD2 expression endows the cellular resistance to ferroptosis inducer. (A–D) Analysis of the CISD2 expression by western blot in HT-1080 (A, C) or HL60 (B, D) cells, GAPDH was used as loading control; (E, F) Cell survival analysis in CISD2-modified HT-1080 cells after the treatment of Erastin (0–10 µM); (G, H) Cell survival analysis in CISD2-modified HL60 cells after the treatment of Erastin (0–15 µM); Cell survival in control cells...

Additional file 2 of Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Yanchun Li, Bing Xu, Xueying Ren, Luyang Wang, Yaqing Xu, Yefeng Zhao, Chen Yang, Chen Yuan, Huanjuan Li, Xiangmin Tong, Ying Wang & Jing Du
Additional file 2: Figure S2. CISD2 expression endows the cellular resistance to ferroptosis inducer. (A–D) Analysis of the CISD2 expression by western blot in HT-1080 (A, C) or HL60 (B, D) cells, GAPDH was used as loading control; (E, F) Cell survival analysis in CISD2-modified HT-1080 cells after the treatment of Erastin (0–10 µM); (G, H) Cell survival analysis in CISD2-modified HL60 cells after the treatment of Erastin (0–15 µM); Cell survival in control cells...

Additional file 5 of Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles

Kohei Yuyama, Hui Sun, Yasuyuki Igarashi, Kenji Monde, Takumi Hirase, Masato Nakayama & Yoichi Makino
Additional file 5: Supplemental Fig. 5. Uncropped membrane images of western blot analysis of the APP Tg serum-derived EVs.

Additional file 2 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 2: Figure S2. Schematic representation of the sequences of the dual luciferase reporter gene vectors for the 4 miRNAs.

Additional file 3 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 3: Figure S3. ShRNA Panx 1-AAV or Ctrl-AAV-labeled EGFP-positive cells have a sound co-localization with S100 β-labeled Schwann cells. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 50 μm. Lower panel, scale bar = 25 μm. n = 3–4 mice/group.

Additional file 6 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 6: Figure S6. CBX and Panx 1-siRNA do not affect TGF-β mRNA expression in LPS-treated Schwann cells. A-B. Pretreatment with CBX (100 mM) for 2 h or Panx 1 siRNA for 36 h, qPCR was used to determine TGF-β mRNA expression levels in LPS-treated Schwann cells. All data are mean ± SEM. n = 4.

Additional file 5 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 5: Figure S5. The effect of Panx 1-siRNA on the expression of Panx 1 in Schwann cells. A, D, and E. mRNA expression of Panx 1, Panx 2, and Panx 3 after treatment with Panx 1 siRNA. B-C. Gel images and quantification of Panx 1 protein levels after treatment with Panx 1 siRNA. F. Panx 1-specific siRNA decreased Panx 1 mRNA expression levels under LPS treatment in Schwann cells. All data are mean...

Additional file 5 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 5: Figure S5. The effect of Panx 1-siRNA on the expression of Panx 1 in Schwann cells. A, D, and E. mRNA expression of Panx 1, Panx 2, and Panx 3 after treatment with Panx 1 siRNA. B-C. Gel images and quantification of Panx 1 protein levels after treatment with Panx 1 siRNA. F. Panx 1-specific siRNA decreased Panx 1 mRNA expression levels under LPS treatment in Schwann cells. All data are mean...

Additional file 1 of Acetaminophen changes the RNA m6A levels and m6A-related proteins expression in IL-1β-treated chondrocyte cells

Jie Gao, Yan Li, Zijin Liu, Dong Wang & Huawu Zhang
Additional file 1: Supplementary Figure 1. MMP-13 levels in the medium of IL-1β-induced C28/I2 cells detected by ELISA.

Registration Year

  • 2022
    86

Resource Types

  • Image
    86

Affiliations

  • Affiliated Hospital of Nantong University
    86
  • Zhejiang University
    76
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    64
  • Huazhong University of Science and Technology
    62
  • Sichuan Agricultural University
    60
  • Nanjing Medical University
    60
  • Guangdong Academy of Medical Sciences
    58
  • Nantong University
    56
  • Jiangsu Province Hospital
    56
  • China Agricultural University
    56