Additional file 1: Figure S1. Phenotype of wild-type (WT), cef3 and cesa9 plants at seedling stage. The seedling of cesa9 is easily broken, while seedlings of WT and cef3 are normal after hand flexing

Additional file 6: Figure S6. Phenotypic comparison among the wild-type and cef3 plants generated by CRISPR–Cas9 system. (A) The phenotype of the wild-type (WT) and cef3 mutants generated by CRISPR-Cas9 system at the mature stage. Scale bars: 10 cm. (B) Folding the internodes of the wild-type, cef3-c2 and cef3-c3. Scale bars: 2 cm (C) The plant height of the wild-type, cef3-c2 and cef3-c3. (D) The extension force of culms in the wide-type, cef3-c2 and cef3-c3...

Northeast Bioengineering Conference Poster 4/23/22

Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 3: Fig. S3. Analysis of the methylation data from GEO database. The difference of the methylation level of H2A.Z and SFRP1 between ICC tissue and normal bile duct was visualized by box plot. (A) GSE38860. (B) GSE49656. *P<0.05, **p<0.01, ***p<0.001.

Additional file 1: Supplementary Figure 1. Kaplan-Meiercurves of OS differences stratified by age, gender, tumor grade and stagebetween both groups in the entire TCGA set.

Additional file 2: Supplementary Figure 2. Partial sensitive compounds.

Additional file 1: Figure S1. The mRNA level of CBFA1 in qRT-PCR (a) and RNA-Seq (b). *P < 0.05.

Additional file 4: Figure S4. HE (scale bar = 50 μm) and Masson (scale bar = 50 μm) staining were performed to evaluate ectopic bone formation in nude mice (n=4).

Additional file 2: Figure S2. CEF3 amino acid sequence alignment of WT and cef3 mutant

Additional file 3: Figure S3. Complementary assay. The phenotype (A) and the plant height (B) show the rescued properties in the complemented plants. Scale bars: 10 cm.

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 3: Figure S3. HE (scale bar = 200 μm) and Masson (scale bar = 50 μm) staining in the rat calvarial defect model (n = 6).

Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Additional file 2: Fig. S2. The site of H2A.Z or HP1α binds to SFRP1 promoter. (A) The ChIP assay analysis of H2A.Z or HP1α enrichment in the SFRP1 promoter in HEK293T cells. IgG served as a negative control. Relative enrichment fold=[%(ChIP/Input)]/[%(IgG/Input)]. (B) Dual luciferase assays demonstrate that H2A.Z attenuates the SFRP1 promoter-controlled luciferase activity in HEK293T and ICC cells (pDNA: empty plasmid vector; pGL3: empty pGL3 plasmid vector; pDNA-H2A.Z: pcDNA-H2A.Z plasmid; SFRP1-pGL3: The SFRP1 promoter...

Additional file 4: Figures S3. KEGG pathway enrichment analysis of the 12 differentially expressed proteins.

Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 1: Figure S1. Phenotype of wild-type (WT), cef3 and cesa9 plants at seedling stage. The seedling of cesa9 is easily broken, while seedlings of WT and cef3 are normal after hand flexing

Additional file 2: Figure S2. CEF3 amino acid sequence alignment of WT and cef3 mutant

Supplementary Material 2

Additional file 2: Figure S2. Results of western blot analysis showed that ETV2 overexpression induced the the activation of p-ERK and p-AKT at days 3, 7 and 14 of osteogenic differentiation. *P < 0.05, compared with the 0 day group.

Northeast Bioengineering Conference Poster 4/23/22

Additional file 3: Fig. S3. Analysis of the methylation data from GEO database. The difference of the methylation level of H2A.Z and SFRP1 between ICC tissue and normal bile duct was visualized by box plot. (A) GSE38860. (B) GSE49656. *P<0.05, **p<0.01, ***p<0.001.

Additional file 4: Figure S4. Deduced CEF3 amino acid sequence alignments for the three homozygous mutants generated by CRISPR-Cas9 system and WT.