Long non-coding RNA (lncRNA) ZFAS1 (zinc finger antisense 1) was demonstrated to play critical roles in various cancer progression. However, the functions of ZFAS in cervical cancers (CC) are unclear. Human CC cell lines were used for in vitro experiments. RT-qPCR (Real Time Quantitative PCR) was performed to detect the expression of ZFAS1, microRNA-190a-3p (miR-190a-3p) and Kruppel-like factor 6 (KLF6). Cell proliferation, invasion and migration assays were used to investigate biological behaviors of CC cells...

This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Additional file 8. The other 4 abundant miRNAs mimics’ effects on mRNA expression of neurotoxic astrocyte markers. Quantitative analysis of mRNA expression of C3 and Lcn2 in astrocytes, neurotoxic astrocytes and neurotoxic astrocytes treated with 50 nM mimics of miR-21-5p(a), miR-22-3p(b), miR-24-3p(c), miR-26a-5p(d) by RT-QPCR. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01; #, p>0.05. C3 complement c3, Lcn2 lipocalin-2.

Additional file 12: Figure S6. Widespread occurrence of rumen bacteria in the communication network. (A) The occurrence of rumen bacteria in the network. (B) The occurrence of bacteria that synthesize AI-2 at the phylum level. (C) The occurrence of bacteria that synthesize and sense AI-2 at the phylum level. (D) The occurrence of bacteria that sense AI-2 at the phylum level. Numbers indicate the numbers of bacterial genomes in which the corresponding functions were found...

Supplementary Material 8

Additional file 5: Figure S5. Composition of splenic immune cells and CD4+ T cell subpopulations in healthy Sigirr +/+ mice and Sigirr −/− mice. (A), Frequencies of major immune populations in spleens between Sigirr +/+ mice (n = 5) and Sigirr −/− mice (n = 5). (B), Absolute number and frequency of granulocytes among 2.5 × 105 live CD45+ leukocytes derived from Sigirr +/+ and Sigirr −/−mice. (C), Representative plots of T cells (CD3+ NK1.1−),...

Additional file 7: Figure S7. Ectopic expression of SIGIRR in purified CD4+ T cells after transfection with retrovirus packaged by phoenix helper-free retrovirus producer lines. The expression of SIGIRR (50 kDa) and GFP (27 kDa) in CD4 T-cell lysates was detected by immunoblotting with GAPDH as a loading control.

Additional file 3: Supplementary Figure S3. The survival curves of necroptosis-score. A-D Kaplan–Meier curve was used to analyze the survival rate of CESC (A), OV (B), UCEC (C), and UCS (D) patients with high or low necroptosis-score. Black curves indicate low necroptosis-score and red curves indicate high necroptosis-score.

Additional file 4: Supplementary Figure S4. Statistical analysis of the number of differential NRGs and prognostic NRGs of the four GCs. Red bars show up-regulated NRGs in cancer tissue. The green bars show NRGs that are downregulated in cancer tissue. The blue bars show the sum of the differential NRGs. Yellow bars indicate prognostic NRGs.

Supplemental material, sj-jpg-2-jet-10.1177_15266028221133700 for Management Strategy and Radiologic Outcomes of Symptomatic Spontaneous Isolated Superior Mesenteric Artery Dissection Based on Angiographic Classification: The Follow-Up Experience in a Single Center by Zihui Yuan, Guofu Hu, Shi Sheng, Yun You and Jian Wang in Journal of Endovascular Therapy

Supplemental material, sj-jpg-2-jet-10.1177_15266028221133700 for Management Strategy and Radiologic Outcomes of Symptomatic Spontaneous Isolated Superior Mesenteric Artery Dissection Based on Angiographic Classification: The Follow-Up Experience in a Single Center by Zihui Yuan, Guofu Hu, Shi Sheng, Yun You and Jian Wang in Journal of Endovascular Therapy

Additional file 6: Fig. S6 Concordance of ctDNA response and radiographic response.

Additional file 7: Fig. S7 Mutational profiles of CSF and plasma samples at baseline.

Additional file 8: Fig. S8 The proportion of clonal and subclonal mutations in CSF and plasma samples at baseline and after 8 weeks of treatment.

Additional file 2: Supplementary figure 2. The relationship between immune-related function and prognosis of CLL patients.

Supplemental material, sj-tif-4-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Efficacy and safety of nivolumab for advanced/recurrent non-small-cell lung cancer: an up-to-date meta-analysis of large-scale phase III randomized controlled trials Supplementary figure 1

Additional file 2: Figure S1. (A) qRT-PCR analysis showed no significant changes in ACE2 expression in colorectal and pancreatic cancer cells after exposure to EPI, PTX or 5-FU. All data are shown as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns P > 0.05 versus control, N = 3.

Additional file 1: Fig. S1. Study participant flow diagram.

Additional file 2: Figure S2. HPLC analysis of carbohydrates in SMI (Lot No. 1907018).

Additional file 1: Figure S1. Research Supplementary Materials. a Process diagram of rat CIH model b Weight Trend Chart of Rat. ****P<0.0001.

Additional file 4: Figure S4. Raw western bloats of NLRP3 (a), Capase-1 (b), ASC(c), IL-1β and β-actin (e) in Figure 6a. The NLRP3, Capase-1, β-actin used 10% Gel; ASC, IL-1β used 12% Gel; all used 0.45 µm PVDF membrane.

Additional file 4: Figure S4. Raw western bloats of NLRP3 (a), Capase-1 (b), ASC(c), IL-1β and β-actin (e) in Figure 6a. The NLRP3, Capase-1, β-actin used 10% Gel; ASC, IL-1β used 12% Gel; all used 0.45 µm PVDF membrane.

Additional file 3: Fig. S2. Multicollinearity analysis for BMI and the MNA-SF.

Supplementary materials to the article ' National survey on unplanned readmissions for diabetic kidney disease: Insight from a new paradigm of obesity'