905 Works

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

Clinical significance and potential molecular mechanism of miRNA-222-3p in metastatic prostate cancer

Yu Sun, Gang Chen, Juan He, Zhi-Guang Huang, Sheng-Hua Li, Yuan-Ping Yang, Lu-Yang Zhong, Shu-Fan Ji, Ying Huang, Xin-Hua Chen, Mao-Lin He & Hao Wu
The clinical significance and underlying molecular mechanism of miRNA-222-3p in metastatic prostate cancer (MPCa) remain unclear. The present study used a large number of cases (n = 1,502) based on miRNA chip and miRNA sequencing datasets to evaluate the expression and diagnostic potential of miRNA-222-3p in MPCa. We applied a variety of meta-analytic methods, including forest maps, sensitivity analysis, subgroup analysis and summary receiver operating characteristic curves, to prove the final results. MiRNA-222-3p was reduced...

High anal swab viral load predisposes adverse clinical outcomes in severe COVID-19 patients

Haibo Li, Lili Ren, Lulu Zhang, Yeming Wang, Li Guo, Conghui Wang, Yan Xiao, Ying Wang, Jian Rao, Xinming Wang, Ying Liu, Chaolin Huang, Xiaoying Gu, Guohui Fan, Hui Li, Binghuai Lu, Bin Cao & Jianwei Wang
To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Distribution characteristics of microbial community structure in atmospheric particulates of the typical industrial city in Jiangsu province, China

Xingcheng Yuan, Guangchao Li, Weihua Yang & Dan Li
In this study, Xuzhou, a typical industrial city in the north of Jiangsu Province, was chosen to investigate the pollution level of atmospheric particulates. The proportion of fine particles (PM2.5) in PM10 is larger than that of coarse particles (about 58%). The physicochemical properties of PM2.5 were analyzed by SEM and EDS. DGGE was used to study the distribution characteristics of bacterial community structure on atmospheric particulates (TSP, PM2.5 and PM10) in different functional areas...

High ultrafiltration rate induced intradialytic hypotension is a predictor for cardiac remodeling: a 5-year cohort study

Jinbo Yu, Xiaohong Chen, Yang Li, Yaqiong Wang, Zhonghua Liu, Bo Shen, Jie Teng, Jianzhou Zou & Xiaoqiang Ding
Intradialytic-hypotension (IDH) is a common complication of hemodialysis. High ultrafiltration rate (UFR) might lead to IDH. However, the relationships between UFR, IDH, and cardiac remodeling among hemodialysis patients in the long-term have not been deeply explored. This retrospective cohort study collected clinical and echocardiographic data. Patients were enrolled from 1 January 2014 to 31 March 2014 and were followed-up for 5-year. Those who suffered from more than four hypotensive events during three months (10% of...

Additional file 7 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 7: Figure S3. miRNAs with different expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

Silencing of let-7b-5p inhibits ovarian cancer cell proliferation and stemness characteristics by Asp-Glu-Ala-Asp-box helicase 19A

Xiujuan Huang, Hongxia Dong, Yang Liu, Fen Yu, Shunshi Yang, Zhen Chen & Jueying Li
The emergence and recurrence of ovarian cancer are associated with ovarian cancer stem cells. For cancer treatment, gene delivery of microbubbles (MB)-mediated microRNA (miRNA) is considered as a promising approach. In this study, our aim is to investigate the effects of MB-mediated let-7b-5p inhibitor on the proliferation and stemness characteristics of ovarian cancer (OVCA) cells. Let-7b-5p inhibitor mediated by MB was prepared (termed MB-let-7b-5p inhibitor), and the effects of MB-let-7b-5p inhibitor and let-7b-5p inhibitor on...

Additional file 2 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 2: Figure S1. Common and specific known miRNAs expressed in wheat crown tissues at four different growth and development stages.

Prediction of bladder cancer outcome by identifying and validating a mutation-derived genomic instability-associated long noncoding RNA (lncRNA) signature

Hao Wu, Zi-Yi Zhang, Ze Zhang, Xin-Ying Xiao, Sheng-Lin Gao, Chao Lu, Li Zuo & Li-Feng Zhang
Bladder cancer is one of the most common malignant tumors worldwide. Accordingly, its incidence and mortality are high. One of the characteristics of cancer is genomic instability. New studies suggest that long non-coding RNAs (lncRNAs) play an important role in maintaining genomic instability. This study aimed to identify a genomic instability-associated lncRNA signature to predict the outcome of patients with bladder cancer. We downloaded data for bladder cancer patients from The Cancer Genome Atlas database...

High hydrostatic pressure (30 atm) enhances the apoptosis and inhibits the proteoglycan synthesis and extracellular matrix level of human nucleus pulposus cells via promoting the Wnt/β-catenin pathway

Zongting Shi, Jun He, Jian He & Yuan Xu
Hydrostatic pressure is known to regulate bovine nucleus pulposus cell metabolism, but its mechanism in human nucleus pulposus cells (HNPCs) remains obscure, which attracts our attention and becomes the focus in this study. Specifically, HNPCs were treated with SKL2001 (an agonist in the Wnt/β-catenin pathway) or XAV-939 (an inhibitor of the Wnt/β-catenin pathway), and pressurized under the hydrostatic pressure of 1, 3 and 30 atm. The viability, apoptosis and proteoglycan synthesis of treated HNPC were...

Long noncoding RNA H19 suppresses cardiac hypertrophy through the MicroRNA-145-3p/SMAD4 axis

Hao Wang, Xiaoqing Lian, Wei Gao, Jie Gu, Haojie Shi, Yao Ma, Yafei Li, Yi Fan, Qiming Wang & Liansheng Wang
Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

The significant immune escape of pseudotyped SARS-CoV-2 variant Omicron

Li Zhang, Qianqian Li, Ziteng Liang, Tao Li, Shuo Liu, Qianqian Cui, Jianhui Nie, Qian Wu, Xiaowang Qu, Weijin Huang & Youchun Wang
The emergence of Omicron/BA.1 has brought new challenges to fight against SARS-CoV-2. A large number of mutations in the Spike protein suggest that its susceptibility to immune protection elicited by the existing COVID-19 infection and vaccines may be altered. In this study, we constructed the pseudotyped SARS-CoV-2 variant Omicron. The sensitivity of 28 serum samples from COVID-19 convalescent patients infected with SARS-CoV-2 original strain was tested against pseudotyped Omicron as well as the other variants...

ETS Proto-Oncogene 1-activated muskelin 1 antisense RNA drives the malignant progression of hepatocellular carcinoma by targeting miR-22-3p to upregulate ETS Proto-Oncogene 1

Guozheng Pan, Jian Zhang, Faping You, Tao Cui, Peng Luo, Shuling Wang, Xiaomei Li & Qingzhong Yuan
Long noncoding RNA muskelin 1 antisense RNA (MKLN1-AS) acted as an oncogenic regulator in hepatocellular carcinoma (HCC). This study was performed to investigate the functional mechanism of MKLN1-AS. MKLN1-AS, microRNA-22-3p (miR-22-3p) and ETS Proto-Oncogene 1 (ETS1) levels were examined using reverse transcription-quantitative polymerase-chain reaction. Protein expression was detected by Western blot. The target relation was analyzed by dual-luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. Cell proliferation ability was determined through cell counting...

Long non-coding RNA growth arrest specific transcript 5 acting as a sponge of MicroRNA-188-5p to regulate SMAD family member 2 expression promotes myocardial ischemia-reperfusion injury

Jin Xu, Dong Yu, Xiaolu Bai & Peng Zhang
The purpose of this work is to probe into the potential role of long non-coding RNA growth arrest specific transcript 5 (lncGAS5)/ microRNA (miR)-188-5p/SMAD2 axis in MIRI. Through ligating the left anterior descending (LAD) coronary artery, MIRI animal model and hypoxia/reoxygenation (H/R) myocardial injury model in vitro were established. Via adenovirus or plasmid transfection, lncGAS5/MiR-188-5p/SMAD2 expression was up-regulated or down-regulated in the study. RT-qPCR was applied to check LncGAS5/MiR-188-5p/SMAD2 mRNA expression, HE staining for histopathological...

MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1

Yan Li, Nianling Yao, Yan Gao, Yunping Wang, Lu Bai, Jia Xu & Haixu Wang
Polycystic ovary syndrome (PCOS) is a common endocrine disorder that poses a great threat to women's health. MiR-1224-5p is downregulated in the follicular fluid of patients with PCOS, but its role remains largely unknown. In this study, mice were treated with dehydroepiandrosterone (DHEA) to establish an in vivo model of PCOS. We found that enhanced activation of NLRP3 inflammasome was accompanied by downregulation of miR-1224-5p in ovarian tissue of PCOS mice. The effect of miR-1224-5p...

MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1

Yan Li, Nianling Yao, Yan Gao, Yunping Wang, Lu Bai, Jia Xu & Haixu Wang
Polycystic ovary syndrome (PCOS) is a common endocrine disorder that poses a great threat to women's health. MiR-1224-5p is downregulated in the follicular fluid of patients with PCOS, but its role remains largely unknown. In this study, mice were treated with dehydroepiandrosterone (DHEA) to establish an in vivo model of PCOS. We found that enhanced activation of NLRP3 inflammasome was accompanied by downregulation of miR-1224-5p in ovarian tissue of PCOS mice. The effect of miR-1224-5p...

Hyperthermia inhibits growth of nasopharyngeal carcinoma through degradation of c-Myc

Xiaole Li, Shichao Duan, Yingjuan Zheng, Yongqiang Yang, Lei Wang, Xinqiang Li, Qing Zhang, Rick F. Thorne, Wencai Li & Daoke Yang
Hyperthermia is a widely used adjunct treatment for different cancers including nasopharyngeal carcinoma (NPC). The protooncogene c-Myc is up-regulated in NPC and its expression is associated with poor prognosis. We hypothesized that c-Myc constitutes an important hyperthermia treatment target, and we investigated its contribution to hyperthermia responses in NPC. The growth of the human NPC cell lines CNE1 and CNE2 was analyzed using CCK-8 and clonogenicity assays after 43 °C hyperthermia, knockdown or overexpression of...

Additional file 2 of A time-course transcriptome analysis of gonads from yellow catfish (Pelteobagrus fulvidraco) reveals genes associated with gonad development

Dong Gao, Junrou Huang, Genmei Lin & Jianguo Lu
Additional file 2: Fig. S1. Bubble plot for the GO enrichment results of DEGs.

Additional file 7 of A time-course transcriptome analysis of gonads from yellow catfish (Pelteobagrus fulvidraco) reveals genes associated with gonad development

Dong Gao, Junrou Huang, Genmei Lin & Jianguo Lu
Additional file 7: Table S6. Bubble plot for the GO enrichment results of gene clusters in female gonad development stages.

Fibrinogen and antithrombin III are associated with in-hospital mortality among critically ill patients with acute kidney injury

Guangyuan Zhang, Lei Zhang, Sun Si, Tiancheng Jiang, Yi Xia, Yongkun Zhu, Xiangyu Zhang, Chi Yao, Ming Chen & Shuqiu Chen
Coagulation factors participates in the inflammatory cascade, known to play a crucial role in the development of acute kidney injury (AKI). Thus, it’s likely that some factors may be associated with AKI. Among them, low levels of fibrinogen and antithrombin III (ATIII) activity have been proved to increase mortality in patients with sepsis. Moreover, they are also reported to be associated with higher incidence of AKI. However, the association between coagulation parameters, especially fibrinogen and...

Additional file 3 of A novel serum spherical lectin from lamprey reveals a more efficient mechanism of immune initiation and regulation in jawless vertebrates

Jiali Lu, Jinsong Duan, Yinglun Han, Meng Gou, Jun Li, Qingwei Li & Yue Pang
Additional file 3: Fig S2. Origin and evolution of LSSL. (A) The phylogenetic tree for the LSSL family based on the neighbor-joining (NJ) method. An NJ tree was constructed using the amino acid sequences of the LSSL proteins. All species represented by abbreviations are shown in Table S2. The bar (0.020) indicates the genetic distance. Different color lines correspond to different species groups. (B) The motif composition of the LSSL family proteins. (C) Model diagram...

Additional file 2 of Global, regional, and national burden of disease study of atrial fibrillation/flutter, 1990–2019: results from a global burden of disease study, 2019

Hong Li, Xuejing Song, Yi Liang, Xue Bai, Wu-Sha Liu-Huo, Chao Tang, Wen Chen & Lizhi Zhao
Additional file 2: Fig. S2. The number and rough ratio of incidence, prevalence, death and dalys in men and women worlwide from 1990 TO 2019.

Additional file 5 of Global, regional, and national burden of disease study of atrial fibrillation/flutter, 1990–2019: results from a global burden of disease study, 2019

Hong Li, Xuejing Song, Yi Liang, Xue Bai, Wu-Sha Liu-Huo, Chao Tang, Wen Chen & Lizhi Zhao
Additional file 5: Fig. S5. Trends in the 5 risk factors attributed to AF/AFL in each of the 5 SDI regions, from 1990 to 2019.

Additional file 1 of Identifying optimal candidates for postoperative adjuvant therapy among regional persistent/recurrent nasopharyngeal carcinoma patients after neck dissection

Sai-Lan Liu, Xiao-Yun Li, Xue-Song Sun, Jing-Yun Peng, Chao Lin, Jin-Jie Yan, Qiu-Yan Chen, Lin-Quan Tang, Shan-Shan Guo, Ling Guo, Li-Ting Liu & Hai-Qiang Mai
Additional file 1.

Additional file 1 of Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis

Bo Cheng, Mengyu Du, Shuxuan He, Lan Yang, Xi Wang, Hui Gao, Haiqing Chang, Wei Gao, Yan Li, Qiang Wang & Yansong Li
Additional file 1: Fig. S1. The gating strategies for the flow cytometry experiments. Data analysis was performed using FlowJo (Ashland, OR). Scatter and staining with the FITC-anti-CD41 and PE-anti-CD40L antibodies were used to gate platelet population. Cells were first gated by regions within a side scatter area (SSC-A) versus forward scatter area (FSC-A) plot, and then through gating those populations in the SSC-A versus FITC-A plots. Activated platelets were defined as FITC-anti-CD41-A positive and PE-anti-CD40L-A...

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  • Sun Yat-sen University
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  • Fudan University
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  • Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Zhejiang University
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  • Sichuan University
    565
  • Shanghai Jiao Tong University
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  • Huazhong University of Science and Technology
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  • Capital Medical University
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