Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

SOCS3 is low-expressed in breast cancer and may be a potential target. Ultrasound targeted microbubble destruction (UTMD) improved the efficiency of gene transfection. We explored the effects of UTMD-mediated transfection of SOCS3 on the biological characteristics and epithelial-mesenchymal transition (EMT) of breast cancer stem cells (BCSCs). The expression of SOCS3 in breast cancer (BC) and its association with prognosis were evaluated by GEPIA and The Cancer Genome Atlas (TCGA) websites. BCSCs were sorted by flow...

This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

The Wandashan accretionary complex (AC) is located in the easternmost part of Northeast China and consists of the Raohe complex in the east and Yuejinshan complex in the west. It represents an excellent location for insights into Paleo-Pacific subduction and accretion processes. However, the protolith (mainly contains Ocean Plate Stratigraphy ‘OPS’ and ophiolite) nature and tectonic evolution of the Wandashan AC are under debate. This contribution reports three representative geological sections, conducts the OPS reconstruction,...

The Wandashan accretionary complex (AC) is located in the easternmost part of Northeast China and consists of the Raohe complex in the east and Yuejinshan complex in the west. It represents an excellent location for insights into Paleo-Pacific subduction and accretion processes. However, the protolith (mainly contains Ocean Plate Stratigraphy ‘OPS’ and ophiolite) nature and tectonic evolution of the Wandashan AC are under debate. This contribution reports three representative geological sections, conducts the OPS reconstruction,...

Additional file 7: Table S6. Bubble plot for the GO enrichment results of gene clusters in female gonad development stages.

Additional file 3: Figure. S2. Relative expression of NlSPARC,NlVg,NlVgR, and NlFoxO in newly emerged adults developed from injected 5th instar nymphs. mRNA levels of NlSPARC, NlVg, NlVgR, and NlFoxO from 5 newly emerged females were analyzed by RT-qPCRwith the 2‒ΔΔCT method from three biological replicates (Mean ± SE). ** above the bars indicate significant differences at P < 0.01 among different treatments by the Tukey’s multiple range tests.

Additional file 9: Fig. S4. Whole-genome variation of two parental lines of QYSM. a, Density and chromosome distribution of polymorphic SNPs between Q9311B and WSSM. b, The principal component analysis. c, Phylogenetic positions of two parental lines (Q9311B and WSSM) in the neighbor-joining tree of 1,946 rice accessions from 3 K-RG, in which the known information on the clades of 3 K-RG is indicated.

Additional file 3: Fig. S1. A, B PCA based on the six pyroptosis-related genes signature. C, D The relationship between the risk score and metastasis. E-G The nomogram calibration curves for predicting 1-, 3-, and 5-year survival in the GSE21257 cohort. H Kaplan–Meier analysis based on the pan-cancer data set and univariate Cox regression analysis of CHMP4C. I The expressions of CHMP4C in tumor and adjacent normal tissues.

Additional file 4: Fig. S2. The PPI network.

Additional file 8: Figure S8. Validation of TPM1 and TREM2 in BV2 cells after transfection with control plasmid, TPM1 plasmid, or TPM1 and Trem2 plasmids. A mRNA level of TPM1 in BV2 cells after transfection with control plasmid, TPM1 plasmid, or TPM1 and Trem2 plasmids. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to TPM1 plasmid, **p < 0.01). Five independent experiments were performed. B–D...

Additional file 11: Figure S7. Integrative analysis of the prognostic value of IRCGS in HCC patients and validation of its co-expressed correlation with five survival-related members of the STING pathway. K-M curve was performed to screen out three key IRCGS significantly associated with OS and DSS in the HCC cohort from TCGA database (A-D). The HCC dataset from ICGC database was used to validate the reliability of the co-expressed correlation of key IRCGS, namely VAV1...

Additional file 11: Figure S7. Integrative analysis of the prognostic value of IRCGS in HCC patients and validation of its co-expressed correlation with five survival-related members of the STING pathway. K-M curve was performed to screen out three key IRCGS significantly associated with OS and DSS in the HCC cohort from TCGA database (A-D). The HCC dataset from ICGC database was used to validate the reliability of the co-expressed correlation of key IRCGS, namely VAV1...

Additional file 15: Figure S11. The raw results of western blotting for the indicated gene.

Additional file 4: Figure S1. The calibration curves at 1-year, 3-year, and 5-year time points were drawn in TCGA-all (A-C) and ICGC (D, E) HCC datasets. The 5-year follow-up data for the ICGC dataset currently is deficient.

Additional file 6: Figure S3. X-title software was utilized to determine the cut-off value of risk score to stratify HCC patients from TCGA-training cohort into low- and high-risk groups. The risk score calculated by gene signature of NRLC3, STING1, TBK1, TRIM21, and XRCC6, was associated with survival probability.

Additional file 2. Risk of bias for each included study.

Additional file 1: Figure S1. Validation of TPM1 overexpression or knockdown in BV2 cells after transfection with TPM1 plasmid or siTPM1. A mRNA level of TPM1 in BV2 cells after TPM1 plasmid transfection. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test or by one-way ANOVA with Tukey’s multiple comparison test (control plasmid vs. TPM1 plasmid, **p < 0.01). Three independent experiments were performed. B, C Western blot analysis...

Additional file 2: Figure S2. TPM1 knockdown reduces inflammation via the PKA/CREB pathway in BV2 cells. A–F qPCR analysis of TPM1, TNF-α, IL-1β, IL-6, COX-2 and iNOS in BV2 cells following treatments with LPS and siTPM1-1, siTPM1-2, or siCTR. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or control, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001;...

Additional file 2: Fig S2. Construct of intermediate cloning vectors for preparing Donor DNA. (A) Fragment A cloned from BW25113-T7 was concatenated to pACYCD-Blank to assemble pACYCD-gene. (B) Reverse-PCR to constructed pACYCD-Donor. (C) The map of Donor DNA, which contains HRL and HRR.

Additional file 1: Fig. S1 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of paternal parent (P1).

Supplemental material, sj-jpg-1-imr-10.1177_03000605221132704 for Congenital esophageal stenosis caused by tracheobronchial remnants: a case report by Zhihong Gao, Lingyan Wang, Huaijun Liu and Xia Zhang in Journal of International Medical Research

Supplemental material, sj-tif-2-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Supplemental material, sj-tif-4-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications