Additional file 8. Figure S8: MSA-2 combined with YM101 therapy promoted immunity-associated gene expression in B16 model. The heatmaps showing the change folds of genes constituting immune signatures.

Additional file 9. Figure S9: The effect of the combination therapy on proliferation and apoptosis markers in B16 and EMT-6 model. (a) Tunel staining in B16 model. (b) Ki67 staining in B16 model. (c) Tunel staining in EMT-6 model. (d) Ki67 staining in EMT-6 model. (e) PCNA staining in EMT-6 model.

Additional file 4: Figure S4. The combination of anti-TGF-β antibody with DAPT synergistically suppresses tumor growth and metastasis. (A) and (B) Grayscale values of NICD and EMCN were quantified by ImageJ. (C) Representative Ki67 staining images of tumor sections from each group are shown from WT mice by IHC. (D) Representative photographs were obtained from the lungs of LLC-injected WT mice treated with vehicle, DAPT alone, anti-TGF-β antibody alone, or a combination of anti-TGF-β antibody...

Additional file 2.

Additional file 3.

Additional file 2: Figure S1. Differential expression of the 11 genes between normal and MM tissues in the (A) GSE6477 and the (B) GSE118985 datasets.

Additional file 4: Figure S3. The relationship between clinical characteristics and risk scores in the training cohort.

Additional file 4: Figure S3. The relationship between clinical characteristics and risk scores in the training cohort.

Additional file 5: Figure S4. Changes in RAS signaling pathway (A and B) and intracellular ATP level (C and D) after drug(s) treatment by invitro experiments.

Additional file 4: Figures S3. KEGG pathway enrichment analysis of the 12 differentially expressed proteins.

Additional file 3: Figures S2. GO enrichment analysis of the 12 differentially expressed proteins.

Additional file 2: Figure S2. Raw western blots of GFAP (a), C3d (b), S100a10(c) and β-actin (d) in Figure 4b 12% Gel, 0.45 µm PVDF membrane.

Additional file 5: Figure S5. Raw western blots of GFAP(a), C3d (b), S100a10(c) and β-actin (d) in Figure 7c 12%Gel, 0.45 µm PVDF membrane.

Additional file 5: Fig. S5. Effect of cilostazol treatment on cardiac function after CLP. a Representative M-mode images of the four different groups. b-e Quantitative analysis of LVEF (b), LVFS (c), LVEDD (d), and LVESD (e) in each group (n = 6). These data indicate no significant difference.

Additional file 6: Fig. S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...

Additional file 1: Supplementary Figure 1.

Additional file 2: Supplementary Figure 2.

Additional file 3: Supplementary Figure 3.

Additional file 1: Fig. S1. The mRNA levels of candidate genes in different molecular subtypes of breast cancer. ER/PR+ (ER/PR+ and HER−, n = 685), HER2+ (n = 168), and TNBC (n = 177). TCGA BRCA ER, PR, and HER2 status were retrieved from Thennavan et al. [21].

Additional file 6. Infiltration of gene expression, overall survival, mutation profile and the comparison of tumor infiltration levels in m6A-related genes with different somatic copy number alterations for LUAD and LUSC in TIMER2.0.

Additional file 7. Infiltration of gene expression, overall survival, mutation profile and the comparison of tumor infiltration levels in m6A-related genes with different somatic copy number alterations for LUAD and LUSC in TIMER2.0.

Additional file 8. The heatmap of correlation between the immune characteristics and the four hub DEMGs in LUAD and LUSC from TISID.

Additional file 10. Overall survival analysis of HPA and validation of gene expressions of 4 hub DEMGs from GSCALite.

Additional file 1. The workflow and study design of the analysis steps.

Additional file 2. Summary of the LC mutation information and the 10 DEMGs mutation information as well as CNV analysis of m6A-related genes in LC (GSCALite).