Additional file 9: Supplementary Figure 9. Forest plot of subgroup analysis for PFS on on TMB detection method.

Additional file 12: Figure S4. Representative immunohistochemical staining images for GSTT1 in bone marrows of Control, Model, S+Rg, S, and Rg groups.

Additional file 1: Figure S1. HPLC analysis of SMI from different batches. (A) HPLC analysis of SMI (Lot No. 1907018). (B) HPLC analysis of SMI (Lot No. 1907229). (C) HPLC analysis of SMI (Lot No. 1907277).

Additional file 2: Fig S2. The solid portion was pancreatic tissues composed of acini, ducts, and islets of Langerhans (hematoxylin and eosin [H&E] stain, ×10).

Additional file 2: Figure S2. SOX21-AS1 silence suppressed the progression of PC. A-G Loss-of-function assays were performed in another two PC cell lines (CFPAC-1 and BxPc3) to further verify the malignant cell behaviors including proliferation, migration, EMT as well as apoptosis upon SOX21 silence treatment. **P < 0.01.

Additional file 3: Figure S3. SOX21-AS1 affected PC cell proliferation, apoptosis, migration, stemness and EMT via modulating SOX21 expression. Rescue experiments in PC cells transfected with shRNA, sh-SOX21-AS1#1 and sh-SOX21-AS1#1 + pcDNA-SOX21, respectively. A-B Cell proliferation detection. C Cell apoptosis detection. D Transwell assays detected the migration ability. E Sphere formation assays detected the stemness. F Western blot analyzed the protein levels of EMT markers and transcription factors. **P < 0.01.

Additional file 2: Fig. S2. Correlation between biventricular global strain and age assessed by Medis. Linear regressions with the 95% prediction band for LV global radial strain (GRS) (a), global circumferential strain (GCS) (b), global longitudinal strain (GLS) (c), and RV free wall- global longitudinal strain (RVFW-GLS) (d).

Additional file 1.

Additional file 1: Figure S1. Feature plots for various genes that help to attribute identities. The intensity of blue is related to the relative expression of the gene in a particular cell.

Additional file 2: Figure S2. Frequency of SIGIRR in memory CD4 T cells in PBMCs of RA patients stratified by CCP expression.

Additional file 3: Figure S3. Cytokine production in SIGIRR-positive or SIGIRR-negative memory CD4 T cells of patients with RA and the healthy population. Related to Fig. 2. (A-B), Memory CD4 T cells were identified as CD4 + CD45RO + CD45RA − previously gated on single live (FVS780 −) lymphocytes (FSC low SSC low). Representative flow cytometry contour plots for intracellular cytokine production among SIGIRR+ memory CD4 T cells in healthy and RA cohorts (n =...

Additional file 4: Figure S4. Targeted disruption of the mouse gene encoding SIGIRR. (A), Schematic of Sigirr deletion in C57BL/6 mice and primers for genotyping. The offspring were born at the expected Mendelian ratios. (B), Sigirr genotyping using the F1, F2 and R1 primers listed in the Materials and Methods. The red arrow shows the knockout band (700 bp), and the blue arrow shows the wild-type band (500 bp). (C), Splenomegaly in naïve SIGIRR KO...

Additional file 1: Fig. S1. The new gastrointestinal occluder device. A Vertical view. B Lateral view.

Additional file 2: Fig. S2. Validation of the expression of miRNA targets between NSCLC cells and HBE cells. (A) CircInteractome database ( https://circinteractome.nia.nih.gov/ ) revealed multiple potential miRNA targets of circ_0017109. (B) qRT-PCR analysis of miRNA expression between NSCLC cells and HBE cells. All data were normalized to the expression level in HBE cells. ***, P < 0.001.

Additional file 6: Fig S5. Elimination of MASP-1 from serum to detect the deposition of LSSL and C3 on the surface of Hela cells. (A) Quantitative analysis of proteins on HeLa cell membranes analyzed by Alexa-488 staining followed by flow cytometry. (B, C) The histogram shows the fluorescence intensity and cell proportions of the above flow cytometry results, respectively. The data are presented as the means ± SDs. (D) Western blotting analysis of depleted and...

Additional file 3: Fig. S1. Phenotypic distribution for 12 traits at the three environments. a, In recombined inbred lines (RILs). b, In backcross F1s hybrids (BCF1s). c, Mid-parental heterosis values. d, Over-standard heterosis values. Over-standard heterosis was calculated by [(BCF1 – QYSM)/QYSM] × 100. RILs derived from a cross between Q9311B and WSSM, BCF1s were hybrids between the RILs and Q9311A. HD, heading date; PH, plant height; PN, panicle number per plant; FLL, flag leaf...

Additional file 4: Fig. S2. High-density linkage map consisting of 855 bins was constructed for the RIL population from a cross between Q9311B and WSSM based on the genotypes at 13,847 segregating genes. a, The recombination bin map of the RIL population (n = 1061), in which the horizontal axis indicates the RILs and the vertical axis indicates genomic regions. Q9311B/Q9311B homozygous type is shown in red, WSSM/WSSM homozygous type is shown in blue, and...

Additional file 5: Fig. S3. Genomic distribution of main-effect QTLs affecting 12 rice yield related traits identified in the RIL and BCF1 populations separately.

Additional file 4: Figure S3. LINC00240 repressed proliferation, induced apoptosis and inhibited migration and invasion of LUAD cells. A LINC00240 expression in A549 cells with LINC00240 stable overexpression or control was detected by qRT-PCR. B Cell proliferation of A549 cells with LINC00240 stable overexpression or control was detected using CCK-8 assays. C Cell proliferation of A549 cells with LINC00240 stable overexpression or control was detected using EdU incorporation assays. Scale bar: 100 µm. Red color...

Additional file 7: Fig. S6. Depletion of LARRPM in LUAD cells enhanced the oncogenic roles of infiltrated M2 macrophages. A–F Conditioned medium (CM) was collected from co-culture of macrophages with HCC827 cells with LARRPM depletion or control HCC827 cells, or collected from only HCC827 cells with LARRPM depletion or control HCC827 cells. A Cell proliferation of HCC827 cells treated with the CM was detected using CCK-8 assays. B Cell proliferation of HCC827 cells treated with...

Additional file 12: Figure S8. The composition of immunohistochemistry (IHC) staining intensity of VAV1 (A), RHOA (B), and ZC3HAV1(C) in eight clinical tumor samples from patients with HCC and their adjacent liver tissues.

Additional file 5: Figure S2. The co-expressed correlation of gene signature of NRLC3, STING1, TBK1, TRIM21, and XRCC6 in HCC patients from TCGA-training (A), TCGA-all (B), and ICGC (C), respectively.

Additional file 6: Figure S3. X-title software was utilized to determine the cut-off value of risk score to stratify HCC patients from TCGA-training cohort into low- and high-risk groups. The risk score calculated by gene signature of NRLC3, STING1, TBK1, TRIM21, and XRCC6, was associated with survival probability.

Additional file 1: Fig S1. Map of plasmids which were constructed for CRISPR. A) Map of pCas, which harbored the temperature sensitive oriR101 with repA101ts, kanamycin resistance gene, the λ-Red operon encoding Gam, Bet, and Exo proteins under the control of arabinose-inducible promoter ParaB, S. pyogenes-derived cas9 driven by endogenous promoters and sgRNA guided to ori-p15a which is under the control of lac operator. B) Map of pTarget-gene, which harbored Chloramphenicol resistance, ori-p15a and sgRNA...

Additional file 1: Fig S1. Map of plasmids which were constructed for CRISPR. A) Map of pCas, which harbored the temperature sensitive oriR101 with repA101ts, kanamycin resistance gene, the λ-Red operon encoding Gam, Bet, and Exo proteins under the control of arabinose-inducible promoter ParaB, S. pyogenes-derived cas9 driven by endogenous promoters and sgRNA guided to ori-p15a which is under the control of lac operator. B) Map of pTarget-gene, which harbored Chloramphenicol resistance, ori-p15a and sgRNA...