Additional file 3: Fig S3. Gene map for targeted Cas9-mediated gene Knock-In. (A) The knock-out site of hemF in BW25113-T7. (B) map of hemF knock-out in ideal condition and the location of PCR product (899 bp) for sequencing.

Additional file 2. Forest plot of sensitivity analysis in MSS subgoup.

Additional file 6.

Supplemental material, sj-tif-5-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Supplemental material, sj-tif-6-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Supplemental material, sj-tif-8-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Supplemental material, sj-tif-9-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Supplemental material, sj-tif-11-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Supplemental material, sj-jpg-13-npx-10.1177_1934578X221130874 for Cryptotanshinone From Salvia miltiorrhiza Inhibits the Growth of Tumors and Enhances the Efficacy of Chemotherapy in a Gastric Cancer Mouse Model by Wenkai Wu, Yezhi Cao, Ling Cheng, Linghu Wang, Qingsheng Yu, Hui Peng, Fuhai Zhou, Haiwei Liu and Qi Zhang in Natural Product Communications

Additional file 4: Fig. S4. The analyses performed on the RNA-seq data of AB2.2 mESCs on differentiation day 2 under normoxia and hypoxia. (A) The related levels of HIF-1 signaling-related genes in AB2.2 mESCs on differentiation day 2 under hypoxia versus normoxia. (B) GSEA showed that the HIF-1 signaling pathway activation was significantly enhanced by hypoxia. (C) The related levels of Wnt/β-Catenin pathway-related genes in AB2.2 mESCs on differentiation day 2 under hypoxia versus normoxia....

Additional file 5: Fig. S5. The Wnt/β-Catenin pathway was affected by hypoxia. (A) The expression of canonical Wnts (Wnt3 and Wnt8a) and (B) Wnt/β-Catenin pathway downstream targets (Sp5 and Cdx1) was downregulated by hypoxia in AB2.2 mESCs undergoing differentiation. (C) The expression of canonical Wnts (Wnt3 and Wnt8a) and (D) Wnt/β-Catenin pathway downstream targets (Sp5 and Cdx1) was downregulated by hypoxia in R1 mESCs undergoing differentiation. (E-G) Expression of nuclear β-Catenin was significantly repressed, while...

Additional file 6: Fig. S6. The Wnt/β-Catenin pathway and AB2.2 mESC differentiation were affected by IWP2. (A) Schematic diagram of mESC differentiation treated with or without IWP2 under normoxia. (B) The expression of Wnt/β-Catenin downstream targets (Sp5 and Cdx1) was severely inhibited by IWP2 in AB2.2 mESCs undergoing differentiation. (C) IWP2 repressed T protein expression (red) on differentiation day 4. Nuclei were stained with DPAI (blue). (D) The ratios of T+ and Sox1+ cells were...

Additional file 8: Fig. S8. The supplementary analyses performed on the RNA-seq data of normoxia, hypoxia, control, and Hif-1α overexpression groups. The volcano plot showed the differentially expressed genes (DEGs) in (A) Hif-1α-OE_vs_Con and (B) HvsN_shHif-1α. |Log2(Fold Change)|>1 and adjusted P-value<0.05 were used as the cutoff criteria. (C) The GO terms of the DEGs upregulated by Hif-1α overexpression. GSEA of (D) apoptosis and (E) cell of AB2.2 mESCs on differentiation day 4 under hypoxia versus...

Additional file 1: Figure S1. The figure file shows the proceeding of soft tissue reconstruction in more detail.

Additional file 12. Figure S12. Histogram representing the proportion of clusters in each group.

Additional file 6: Figure S2. Western blotting of recombinant protein of CsFLS2 and CsFLS3.

Additional file 4: Figure S4. TPM1 knockdown exacerbates inflammation in the TREM2−/− mouse retina. A, B Western blot analysis (A) and quantification of TPM1 (B) in the retina of C57BL/6J (WT) and TREM2−/− mice. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (WT vs. TREM2−/−, **p < 0.01). C, D Western blot analysis (C) and quantification of TREM2 (D) in WT mice following LPS and siCTR or siTPM1-1...

Additional file 6: Figure S6. TPM1 knockdown elicits inflammation-related transcriptomic alterations in the WT retina. A, B DEGs associated with the phagosome formation pathway (A) and neuroinflammation signaling pathway (B) in WT mice after treatments with LPS and siTPM1-1 or siCTR.

Additional file 7: Figure S7. Validation of the CREB inhibitor 666-15 in TREM2−/− mice following LPS treatment. A–C Western blot analysis (A) and quantification of p-CREB and CREB (B, C) in TREM2−/− mice after treatments with LPS and 666-15, a potent and selective CREB inhibitor. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (compared to LPS, ****p < 0.001). n = 4 mice in each group.

Additional file 7: Figure S7. Validation of the CREB inhibitor 666-15 in TREM2−/− mice following LPS treatment. A–C Western blot analysis (A) and quantification of p-CREB and CREB (B, C) in TREM2−/− mice after treatments with LPS and 666-15, a potent and selective CREB inhibitor. Data are presented as mean ± SEM and analyzed by unpaired two-tailed Student’s t test (compared to LPS, ****p < 0.001). n = 4 mice in each group.

Additional file 8: Figure S8. Validation of TPM1 and TREM2 in BV2 cells after transfection with control plasmid, TPM1 plasmid, or TPM1 and Trem2 plasmids. A mRNA level of TPM1 in BV2 cells after transfection with control plasmid, TPM1 plasmid, or TPM1 and Trem2 plasmids. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to TPM1 plasmid, **p < 0.01). Five independent experiments were performed. B–D...

Additional file 9: Figure S9. DNA sequencing for detecting Rd8-associated single nucleotide deletion. F: Forward; R: Reverse.

Supplemental material, sj-png-1-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Supplementary retrieval methods Supplementary tables 1-2 Supplementary figures 1-10 PRISMA

Supplementary retrieval methods Supplementary tables 1-2 Supplementary figures 1-10 PRISMA