Supplemental material, sj-tif-3-jet-10.1177_15266028221125581 for Network Meta-Analysis of All Available Regimens Based on Drug-Coated Balloon Angioplasty and Laser Atherectomy for Femoropopliteal In-Stent Restenosis by Chaowen Yu, Wenbo Tang, Tiancai Ren, Qiwei Chen, Ran Lu and Yong Gao in Journal of Endovascular Therapy

Supplemental material, sj-tif-4-jet-10.1177_15266028221125581 for Network Meta-Analysis of All Available Regimens Based on Drug-Coated Balloon Angioplasty and Laser Atherectomy for Femoropopliteal In-Stent Restenosis by Chaowen Yu, Wenbo Tang, Tiancai Ren, Qiwei Chen, Ran Lu and Yong Gao in Journal of Endovascular Therapy

Diabetic kidney diseases (DKD) were the leading cause of End-stage renal diseases worldwide. Albuminuria was a target for treatment in DKD and decreasing albuminuria was particularly important for improving its prognosis. However, there is still a lack of specific treatment for DKD. We conducted a prospective, crossover, open-label study to investigate the effect of amiloride in patients with DKD. Safety and efficacy were assessed by monitoring urine protein creatinine ratio(uPCR), urinary albumin creatinine ratio (uACR),...

Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme-producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected to...

Supplemental material, sj-tif-4-vmj-10.1177_1358863X221132591 for Circular RNA suppression of vascular smooth muscle apoptosis through the miR-545-3p/CKAP4 axis during abdominal aortic aneurysm formation by Jiabo Wei, Hui Wang and Qinming Zhao in Vascular Medicine

Supplemental material, sj-tif-5-vmj-10.1177_1358863X221132591 for Circular RNA suppression of vascular smooth muscle apoptosis through the miR-545-3p/CKAP4 axis during abdominal aortic aneurysm formation by Jiabo Wei, Hui Wang and Qinming Zhao in Vascular Medicine

Supplmentary figure 1

Additional file 8: Fig S7. Elimination of LSSL from serum to detect the deposition of VLRB and C1q on the surface of HeLa cells. (A) Immuno-fluorescence detection of VLRB and C1q deposited on the surface of HeLa cells treated with lamprey serum and LSSL-depleted serum. Scale bars, 10 µm. (B) A histogram shows the statistics of the above-mentioned results. The data are presented as the means ± SDs. (C) Western blotting analysis with mouse anti-VLRB...

Additional file 6: Fig S5. Elimination of MASP-1 from serum to detect the deposition of LSSL and C3 on the surface of Hela cells. (A) Quantitative analysis of proteins on HeLa cell membranes analyzed by Alexa-488 staining followed by flow cytometry. (B, C) The histogram shows the fluorescence intensity and cell proportions of the above flow cytometry results, respectively. The data are presented as the means ± SDs. (D) Western blotting analysis of depleted and...

Additional file 4: Fig S3. Cryo-EM analysis. (A) A flow-chart of cryo-EM data processing. (B) Subunit structure in LSSL trimer. (C) Fold diagram for the structure of LSSL. (D) FSC curve (gold standard FSC) as a function of before and after mask application.

Supplemental material, sj-jpg-1-ijd-10.1177_10567895221135054 for Investigation into a comprehensive forming limit curve considering multi-parameters effects for semi-cured FMLs through uniform pressure loading test by Meng Zhang, Hasnain Ali Mirza, Ye Chi Zhang, Muhammad Nazim Tabasum, HL Lang and Ping Zheng Zou in International Journal of Damage Mechanics

Supplementary figures 1-5 Supplementary tables 1-8

Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 7: Fig. S6. A-B Scratch wound-healing assays showed cell migration capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. C-D Transwell migration and matrigel invasion assays showed cell migration and invasion capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. E Representative images (fluorescence) of lung metastatic...

The files include supplementary tables and detailed methods used in the manuscript, such as human acetabular cartilage dissociation and nuclei isolation and library construction, and 4 supplementary figures.

The files include supplementary tables and detailed methods used in the manuscript, such as human acetabular cartilage dissociation and nuclei isolation and library construction, and 4 supplementary figures.

The mechanisms of nephroprotection in non-diabetic chronic kidney disease (CKD) models by sodium-glucose cotransporter 2 (SGLT2) inhibitors are not well defined. Five groups were established: sham operated rats, placebo treated rats with 5/6 nephrectomy (5/6Nx); 5/6Nx + telmisartan (5mg/kg/day), 5/6Nx + empagliflozin (3mg/kg/day); 5/6Nx + empagliflozin (15mg/kg/day). Treatment duration was 95 days. Empagliflozin showed a dose-dependent beneficial effect on the change from baseline of creatinine clearance (Ccr). The urinary albumin to creatinine ratio likewise improved...

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Supplemental material, sj-jpg-1-bmi-10.1177_11772719221141525 for Advantages and Limitations of Monitoring Circulating Tumor DNA Levels to Predict the Prognosis of Patients Diagnosed With Gastric Cancer by Wan He, Jingxin Yang, Xiao Sun, Shunda Jiang, Jinchan Jiang, Ming Liu, Tianhao Mu, Yingmei Li, Xiaoni Zhang, Jingxian Duan and Ruilian Xu in Biomarker Insights

Additional file 1: Supplementary Figure. S1. Alignment of multiple CqSPL and select SBP domain amino acid sequences.

Additional file 3. Tucidinostat plus checkpoint blockade induces improved therapeutic efficacy. a Schema of the experiment. For bioluminescence imaging (BLI) in vivo, mouse 4T1-luc (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). Tumors volume...

Additional file 4: Fig S3. Cryo-EM analysis. (A) A flow-chart of cryo-EM data processing. (B) Subunit structure in LSSL trimer. (C) Fold diagram for the structure of LSSL. (D) FSC curve (gold standard FSC) as a function of before and after mask application.

Additional file 2: Fig. S2. Data processing for weighted correlation network analysis (WGCNA) and Gene ontology enrichment categories for transcripts in the most significantly correlated module #7EC563. A Analysis of network topology for 1-20 soft-thresholding powers. B Clustering dendrogram of transcripts, with dissimilarity based on the topological overlap, and assigned merged and original module colors. C Gene ontology enrichment categories for transcripts in the most significantly correlated module #7EC563.