Additional file 11: Fig. S5. Speculation of the subpopulation source of the parental allele at all 14,397 segregating genes by comparing the allele frequencies of Q9311B and WSSM in 772 Geng and 1,174 Xian accessions from 3 K-RG.

Severe hydronephrosis increases the risk of urinary tract infection and irretrievable renal fibrosis. While TGFβ1-mediated fibrotic changes in proximal tubular epithelial cells and fatty acid oxidation (FAO) deregulation contribute to renal fibrosis and hydronephrosis. Firstly, a few elements were analyzed in this paper, including differentially-expressed long non-coding RNAs (lncRNAs), and miRNAs correlated to CPT1A, RXRA, and NCOA1. This paper investigated TGFβ1 effects on lncRNA FABP5P3, CPT1A, RXRA, and NCOA1 expression and fibrotic changes in HK-2...

Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Supplementary Material 3

Additional file 3: Figure S3. Transfection efficiency of Rab27a in HCC cells. A Rab27a levels in HCC cells was detected by RT-qPCR and western bot analyses after Rab27a silencing. N = 3. **P < 0.01.

Additional file 4: Figure S4. PRR34-AS1 enhances THLE-3 cell malignant phenotypes via elevating the expression of Rab27a. A–F Rescue experiments were carried on in THLE-3 cells transfected with NC, PRR34-AS1, PRR34-AS1 + sh/Rab27a#a to assess cell proliferative, migratory and invasive as well as EMT phenotypes. N = 3. Scale bar = 50 µm. **P < 0.01.

Additional file 2: Fig. S2. The Sanger sequencing results of the mutation sites of the 15 mutant individual plants, in which the results of SNP-5 and SNP-6 are different from the targeted sequencing results, these two mutant individual plants are excluded, and the rest are the same as the targeted sequencing results, which are true variation.

Additional file 1: Additional Fig. 1. Age-distribution of injury mortality in Xi’an, 2005-2020

Additional file 7: Fig. S7. Only a few proteins and transcripts have potential impacts on TMA production. A Ranked abundance plot of proteins and transcripts in duck livers. The top 10 most abundant proteins accounted for 12.7% of the total proteins, and the top 10 most abundant transcripts covered 33.8% of all transcripts in this tissue. B Changes in protein and transcript abundances. The upper-right panel shows the proportion of significantly altered proteins and transcripts.

Additional file 6: Figure S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...

Supplementary Material 3

Supplemental material, sj-eps-4-tam-10.1177_17588359221133546 for Circulating tumour DNA biomarkers in savolitinib-treated patients with non-small cell lung cancer harbouring MET exon 14 skipping alterations: a post hoc analysis of a pivotal phase 2 study by Yongfeng Yu, Yongxin Ren, Jian Fang, Lejie Cao, Zongan Liang, Qisen Guo, Sen Han, Zimei Ji, Ye Wang, Yulan Sun, Yuan Chen, Xingya Li, Hua Xu, Jianying Zhou, Liyan Jiang, Ying Cheng, Zhigang Han, Jianhua Shi, Gongyan Chen, Rui Ma, Yun Fan,...

Additional file 1. Fig. S1: Low doses of SI-2 exhibit better tumor-suppressive activity than high doses of SI-2. A Reduction in luciferase activity in E0771 tumors in B6 albino mice by SI-2 (2.5 mg/kg and 5 mg/kg) treatment compared with vehicle treatment. Quantification of luciferase activity in the E0771 breast tumors shown in Panel A. B–D. Numbers of CD4+ T cells (B), CD8+ T cells (C), and CD56+ NK cells (D) in E0771 breast tumors...

Additional file 2: Fig. S2. The site of H2A.Z or HP1α binds to SFRP1 promoter. (A) The ChIP assay analysis of H2A.Z or HP1α enrichment in the SFRP1 promoter in HEK293T cells. IgG served as a negative control. Relative enrichment fold=[%(ChIP/Input)]/[%(IgG/Input)]. (B) Dual luciferase assays demonstrate that H2A.Z attenuates the SFRP1 promoter-controlled luciferase activity in HEK293T and ICC cells (pDNA: empty plasmid vector; pGL3: empty pGL3 plasmid vector; pDNA-H2A.Z: pcDNA-H2A.Z plasmid; SFRP1-pGL3: The SFRP1 promoter...

Additional file 10: Supplementary Figure S10. Spearman correlation coefficient between 22 immune cells was calculated for CESC (A), OV (B), UCEC (C), and UCS D. Red bubbles indicate positive correlations and blue bubbles indicate negative correlations. The numbers in the bubbles represent the correlation coefficients.

Additional file12: Supplementary Figure S12. Spearman correlation between NRGs expression level and IC50 level of drugs. A Bubble chart for CESC. B Bubble chart for OV. C Bubble chart for UCEC. D Bubble chart for UCS. The bubble color indicates the degree of correlation index. The bubble size indicates the P-value. The correlation with P<0.05 & |Cor|>0.3 were retained to produce the figure.

Additional file 4: Figure S4. Targeted disruption of the mouse gene encoding SIGIRR. (A), Schematic of Sigirr deletion in C57BL/6 mice and primers for genotyping. The offspring were born at the expected Mendelian ratios. (B), Sigirr genotyping using the F1, F2 and R1 primers listed in the Materials and Methods. The red arrow shows the knockout band (700 bp), and the blue arrow shows the wild-type band (500 bp). (C), Splenomegaly in naïve SIGIRR KO...

Additional file 8: Fig S7. Elimination of LSSL from serum to detect the deposition of VLRB and C1q on the surface of HeLa cells. (A) Immuno-fluorescence detection of VLRB and C1q deposited on the surface of HeLa cells treated with lamprey serum and LSSL-depleted serum. Scale bars, 10 µm. (B) A histogram shows the statistics of the above-mentioned results. The data are presented as the means ± SDs. (C) Western blotting analysis with mouse anti-VLRB...

Additional file 3: Figure S1. Time-dependent ROC analysis for 5-year Mortality. A The cut-off value is 1.0 for men; B. The cut-off value is 0.8 for women.

Additional file 4: Figures S3. KEGG pathway enrichment analysis of the 12 differentially expressed proteins.

Supplementary figure 1-3 Supplementary table 1-2

Supplemental material, sj-tiff-1-imj-10.1177_10815589221149187 for Thyroid-stimulating hormone within the normal reference range has a U-shaped association with the severity of coronary artery disease in nondiabetic patients but is diluted in diabetic patients by Wenyuan Li, Qingyuan He, Haoxuan Zhang, Shan Shu, Lijun Wang, Yue Wu, Zuyi Yuan and Juan Zhou in Journal of Investigative Medicine

Supplemental material, sj-tiff-1-imj-10.1177_10815589221149187 for Thyroid-stimulating hormone within the normal reference range has a U-shaped association with the severity of coronary artery disease in nondiabetic patients but is diluted in diabetic patients by Wenyuan Li, Qingyuan He, Haoxuan Zhang, Shan Shu, Lijun Wang, Yue Wu, Zuyi Yuan and Juan Zhou in Journal of Investigative Medicine

Supplemental material, sj-png-1-aim-10.1177_09645284221107685 for Effect of trigger point acupuncture on pain and functional activity in patients with chronic non-specific low back pain: a randomised controlled trial by Hong Liu, Ya-Ping Li, Mei-Jin Hou, Wu-Jie Huang, Xiao-Lin Chen, Zhen Gao and Zheng Jiang in Acupuncture in Medicine

Supplementary Materials for 'Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil', as published in International Journal of Systematic and Evolutionary Microbiology