Supplemental material, sj-jpg-3-jcb-10.1177_0271678X221145090 for Progranulin released from microglial lysosomes reduces neuronal ferroptosis after cerebral ischemia in mice by Tingting Chen, Rubing Shi, Qian Suo, Shengju Wu, Chang Liu, Shuxian Huang, Khan Haroon, Ze Liu, Yuyan He, Heng-Li Tian, Yongting Wang, Yaohui Tang, Guo-Yuan Yang and Zhijun Zhang in Journal of Cerebral Blood Flow & Metabolism

Supplmentary figure 1

The files include supplementary tables and detailed methods used in the manuscript, such as human acetabular cartilage dissociation and nuclei isolation and library construction, and 4 supplementary figures.

The files include supplementary tables and detailed methods used in the manuscript, such as human acetabular cartilage dissociation and nuclei isolation and library construction, and 4 supplementary figures.

Additional file 8. Figure S2: Evaluation of RNA modification score (RNAMS) risk prediction model. a–b Survival curves for GSE84437 (a) and GSE62254 (b); c–d Risk score distribution for GSE84437 (i) and GSE62254 (j) expression profiles; e–f ROC curves for GSE84437 (e) and GSE26901 (f).

Additional file 1: Supplementary figure 1.

This study aimed to investigate the association between different metabolic syndrome-body mass index (MetS-BMI) phenotypes and the risk of kidney stones. Participants aged 20–80 years from six consecutive cycles of the NHANES 2007–2018 were included in this study. According to their MetS status and BMI, the included participants were allocated into six mutually exclusive groups: metabolically healthy normal weight (MHN)/overweight (MHOW)/obesity (MHO) and metabolically unhealthy normal weight (MUN)/overweight (MUOW)/obesity (MUO). To explore the association between...

Additional file 6. Nerve endings terminate adjacent to ChAT-positive or α7nAChR-positive cell in spleen. Synaptophysin-positive (green) nerve endings were found in close proximity to ChAT-positive (A: red) or α7nAChR-positive (B: red) in LIPUS group of mice (especially white arrow). In statistical analysis, the number of target cell in LIPUS group is normalized to EAM group (bottom). (n=4) * represents LIPUS-0.35 MPa vs. EAM. (Data were shown as the mean ± SD, unpaired T test, Mann-Whitney...

Additional file 2. LIPUS stimulated localization on the spleen and measurement of mouse spleen dimensions. Mice were anesthetized with isoflurane before LIPUS stimulation. Since the spleen is shallow, it can be clearly outlined and marked through the thin skin (A-B). To confirm the spleen's location, the mouse skin was turned over (C). The spleen is consistently located halfway between the shoulder and hip joints of the animal, so the landmarks can be used to target...

Additional file 5. Effect of LIPUS on the immunohistochemical staining of CX3CR1 in myocardium. Representative immunohistochemical staining of CX3CR1 in myocardium of control, EAM, and LIPUS group (n=4). Scale bar represents 50 μm. EAM: Experimental Autoimmune Myocarditis; LIPUS: low-intensity pulsed ultrasound.

Additional file 7. The effect of splenic denervation on vessels of EAM mice. A. HE staining for spleen histological signs of EAM mice and EAM+SDN mice. B. Representative images of whole spleen sections of EAM mice and EAM+SDN mice. Blue, nuclear staining. Green, CD31 is a sensitive and specific marker for vascular endothelial injury (especially white arrow). EAM: Experimental Autoimmune Myocarditis; SDN: Splenic Denervation; HE: hematoxylin and eosin.

Additional file 2: Figure S1. IRAK-M expression is inducible by multiple stimuli in A549 cells. IRAK-M protein expression at 0, 6, 12, 24 h after (a) IL-1β (1 ng/ml), (b) TNF-α (10 ng/ml), (c) IL-33 (100 ng/ml) and (d) HDM (10 μg/ml) exposure. IRAK-M protein expression was normalized to GAPDH. Values are expressed as mean ± SEM (n = 3). *P < 0.05.

Supplemental material, sj-jpg-1-jcb-10.1177_0271678X231165606 for Microvascular dysfunction associated with unfavorable venous outflow in acute ischemic stroke patients by Xiaoshuang Li, Liping Lin, Jian Zhang, Yuhua Fan, Shihui Xing, Li Jiang, Zhiyun Yang, Jing Zhao and Jingjing Li in Journal of Cerebral Blood Flow & Metabolism

Additional file 2: Figure S2. The ROC curves of Resnet101 and VGG-19.

Additional file 4: Figure S1. The m6A sites of TMBIM6 predicted by RMBase v2.0.

Additional file 5: Figure S2. TMBIM6 was the potential target of METLL3 by M6A2Target.

Additional file 1. S-plots in the negative and positive ion modes. A: S-plot of Control and Model group; B: S-plot of Model and Sch group; C: S-plot of Control and Model group; D: S-plot of Model and Sch group. (A and Bin positive ion mode, C and D in positive ion mode).

Additional file 2: Figure S2. The Cobb angle, sagittal parameters and LL before (A,C and E) and after (B,D and F) operation of case 23.

Additional file 2: Figure S2. FADS2 correlate with the majority of tumor infiltrating immune cells utilizing XCELL.

Additional file 2: Figure S2. FADS2 correlate with the majority of tumor infiltrating immune cells utilizing XCELL.

Additional file 3. Performance metrics generated by the ridge regression (RR) algorithm for within-herd validation.

Additional file 2: Fig. S2. Binding of KPNA2 to STAT3 was increased under hypoxia for 24 h relative to normoxia. (A)Western blotting analysis was used to detect KPNA2 of IP STAT3 hypoxia for 24 h vs under normoxia. Relative quantitative statistical analysis was performed on protein expression levels. Each experiment was repeated three times. Each experiment was repeated three times. * p<0.05.

Additional file 5: Fig. S3. (a) None of the mRNA expressions of ABCB1, ABCB9, ABCC1, ABCC2, ABCC3, ABCC5, ABCF2 and ABCG2 is significantly different between the metastasized and non-metastasized CRC patients. M0: no metastasis, M: metastasis. (b) None of the mRNA expressions of ABCB1, ABCB9, ABCC1, ABCC2, ABCC3, ABCC5, ABCF2 and ABCG2 is significantly different between the recurrent and non recurrent CRC patients post initial therapy. (c) None of the mRNA expressions of ABCB1, ABCB9,...

Additional file 6: Fig. S4. The percent survival of the CRC patients with higherABCB1, ABCB9, ABCC1, ABCC2, ABCC3, ABCC5, ABCF2 or ABCG2 mRNA is basically the same as those with lower mRNA expression.

Additional file 8: Fig. S6. Caco-2 and RKO cells were treated with Tm (10 μg/mL) or STF (25, 50, 100, 200 and 400 μM). IRE1α pathway is inactivated upon the treatment of 200 or 400 μM of STF, by which ABCC10 is elevated. Therefore, 200 μM of STF was used in the further experiments.