Supplementary Material 2

Additional file 1: Uncropped images for Alizarin red staining

Additional file 4: Figure S4. Chromatogram analysis of various lipid components. A A total of 131 differential lipids, including 53 TG (40.46%), 24 PE (18.32%), 10 PC (7.63%), 10 PS (7.63%), 7 SM (5.34%), 7 PA (5.34%) and 20 other lipids (15.27%), were identified. B TG, C PE, D PC, E PS, F PA, G SM, H Other lipids. TG, triglyceride; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PS, phosphatidylserine; PA, phosphatidic acid; SM, sphingomyelin; LPE, lysophosphatidylethanolamine; LPC,...

Additional file 5: Supplementary Fig. 5. Full-length blots of Figure 8A.

Additional file 17: Fig. S15. Original data: Uncropped western blot images of Fig. 6a.

Additional file 18: Fig. S16. Original data: Uncropped western blot images of Fig. 6b. The framed regions of the blots in Fig. S9~S16 are used in the manuscript. Since the blots were cut prior to hybridisation with antibodies, the provided uncropped images are not of full length.

Additional file 13: Fig. S11. Original data: Uncropped western blot images of Fig. 3b.

Additional file 4: Figure S3. MiR-194-5p affects the expression of SOCS2 which reverses the effects of miR-194-5p on cell proliferation and migration. (A) and (B), the number of colonies or cells was counted in the control group, miR-194-5p mimic or miR-194-5p inhibitor group, miR-194-5p mimic or miR-194-5p inhibitor group and SOCS2 overexpression plasmid or si-SOCS2 co-transfected group by colony formation and Transwell assays. (C) HE staining (scale bar = 100 μm) and the expression of...

Additional file 5: Figure S4. CircPVRL3 inhibits the cell proliferation in PDAC cells. (A) EdU assay, (B) colony formation assay, and (C) CCK-8 assay were performed to detect the function of circPVRL3 which effected on the cell proliferation in PANC-1 and CFPAC-1 cells. Scale bar = 100 μm. *p < 0.05; **p < 0.01; ***p < 0.001.

Additional file 2: Supplementary figure 2. Landscape of the methylation levels of 5mC/m6A regulators across pan-cancer. (A, B) Correlations of methylation and mRNA expression of 5mC/m6A regulators. (C, D) Differential methylation of 5mC/m6A regulators between tumor and paired normal tissues in each cancer type. (E, F) Survival differences between high and low methylation of 5mC/m6A regulators in each cancer type, reflecting survival affected by methylation.

Additional file 5: Supplementary figure 5. Prognostic value of 5mC/m6A regulators. (A) Heatmap showing the univariate cox regression analysis of 5mC/m6A regulators with OS across pan-cancer. (B) Forest plot for the univariate cox regression analysis of 5mC/m6A regulators with HCC patients’ OS.

Additional file 2.

Additional file 2: Figure S1. The study screening and selection process.

Additional file 3: Figure S2. Results of the risk of bias assessment of the eighteen studies included in this systematic review.

Additional file 7: Supplementary figure 7. Subgroup analysis of HCC patients stratified by distinct clinicopathological factors. Kaplan-Meier curves of OS between high and low EME HCC patients in each subgroup of (A, B) age ≥65 and <65, (C, D) female and male, (E, F) G1-2 and G3-4, (G, H) stage I-II and stage III-IV, (I, J) T1-2 and T3-4, and (K, L) HBV infection and non-infection.

Additional file 1: Supplementary Fig. 1. Expression and prognosis of TSPs in glioma.

Additional file 1: Supplementary Fig. 1. Expression and prognosis of TSPs in glioma.

Additional file 2: Supplementary Fig. 2. Analysis of the Spearman correlation coefficients between the expression of TSP2 protein and the age of patients.

Additional file 3: Supplementary Fig. 3. The source of the TSP2 protein in surgical specimens.

Additional file 4: Supplementary Fig. 4. Effects of different concentrations of TSP2 protein on the proliferation of U251 cells.

Additional file 3: Supplementary Fig. 3. Subgroup analysis for Forns score

Additional file 2. PAQR4 promoted the migration and invasion of HCC cells in vitro (A–B).

Additional file 2. PAQR4 promoted the migration and invasion of HCC cells in vitro (A–B).

Additional file 2: Figure S2. (A-B) The osteosarcoma cells (3000 cell/well) with or without LINC00629 overexpression were tested for the cell growth in the colony formation assay. Viable colonies after 1 week were counted and were shown(A). Data are depicted as bar graphs (B). (C-D) The migration of the indicated cells was detected by Transwell assays. Represented images of crystal violet-stained culture plates were shown (C). Data are depicted as bar graphs (D). Data in...

Additional file 6: Figure S6. (A-B) The osteosarcoma cells (3000 cell/well) with or without KLF4 knockdown were tested for the cell growth in the colony formation assay. Viable colonies after 1 week were counted and were shown (A). Data are depicted as bar graphs (B). (C-D) The migration of the indicated cells was detected by transwell assays. Represented images of crystal violet-stained culture plates were shown (C). Data are depicted as bar graphs (D). (E-F)...