Mitochondrial injury-triggered podocyte apoptosis is a major risk factor for diabetic nephropathy (DN). However, the detailed relationship between mitochondrial homeostasis and podocyte apoptosis remains unclear. The present study aimed to explore the role and functional mechanism of germacrone in DN in type I diabetes (type I DN). A mouse model of type I DN was established by injecting streptozocin, and a podocyte injury model was constructed using high glucose (HG) induction. Histopathology was detected by...

Atherosclerosis (AS) is a chronic inflammatory disease involving endothelial dysfunction, and is one of the main causes of death from cardiovascular disease (CVD). Long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) is overexpressed in the serum of CVD patients. However, the mechanism by which lncRNA PVT1 functions in AS remains unknown. Our research was designed to probe interactions involving lncRNA PVT1 and oxidized low-density lipoprotein (ox-LDL)-stimulated endothelial cell injury in AS. lncRNA PVT1 expression...

Atherosclerosis (AS) is a chronic inflammatory disease involving endothelial dysfunction, and is one of the main causes of death from cardiovascular disease (CVD). Long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) is overexpressed in the serum of CVD patients. However, the mechanism by which lncRNA PVT1 functions in AS remains unknown. Our research was designed to probe interactions involving lncRNA PVT1 and oxidized low-density lipoprotein (ox-LDL)-stimulated endothelial cell injury in AS. lncRNA PVT1 expression...

Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Additional file 5: Supplementary Figure 1. A The enrichment of 4 most significantly changed circRNAs (FC > 4, P < 0.05) of the circRNA sequencing in the exosomes of 6 LUAD patients (3 PR and 3 PD) accepting the PD1 blockade was measured by qRT-PCR. B Correlation between exosomal circRNA-seq results of circZNF451 and the expression of circZNF451 in the tumor tissues of six LUAD patients was analyzed by Spearman correlation analysis. C The expression...

Additional file 6: Supplementary Figure 2. A Infiltration of CD4+T, CD8+T, Foxp3+Treg, CD56+NK and CD11C+dendritic cells and CD86+ and CD163+ macrophages in the circZNF451high and circZNF451low group was presented in the violin plot. B The transfection efficiency of circZNF451 in LLC was measured by qRT-PCR. C The construction of A549-shcircZNF451, H1299-shcircZNF451, H1395-circZNF451 and H1975-circZNF451 cell lines was confirmed by qRT-PCR. D IFN-γ and GZMB levels in the supernatant of CD8+ T cells were measured via...

Additional file 8: Supplementary Figure 4. A After coculturing with A549 for 48h, RNA pull down was performed in macrophages transfected with TRIM56mutant to confirm the interaction between circZNF451 and TRIM56mutant. B The co-IP assay was used to verify the interaction between FXR1 and TRIM56 in macrophage after coculturing with A549/H1299-shcircZNF451 cells for 48h. C The M2 phenotype markers Arg1 and IL-10 were detected by qRT-PCR in LPS stimulated macrophage-siFXR1 cells stimulated by LPS. D...

Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...

Neoadjuvant chemotherapy has been widely used in locally advanced and inflammatory breast cancer. Generally, complete pathological response after neoadjuvant chemotherapy treatment predicts survival. Studies have shown that patient-derived organoids can be used in cancer research and drug development. Therefore, we aimed to generate a living organoid biobank from biopsy samples to predict the response of patients to neoadjuvant chemotherapy. We generated a living organoid biobank from locally advanced breast cancer patients receiving neoadjuvant chemotherapy. When...

Additional file 16: Figure S7. c-Myc promotes circPVT1 generation by recruiting SRSF1 to couple transcription to splicing. A. The luciferase reporter gene activity of the PVT1 promoter was analyzed in NPC cells after overexpression of c-Myc. Data were represented as mean ± SD. ***, p < 0.001. B. The binding of transcription factor c-Myc to the PVT1 promoter detected by ChIP assay. Three sites on the PVT1 promoter were identified as potential c-Myc binding sites...

Additional file 7: Figure S7. (A) Phylogenetic tree of CEF3 in rice and Arabidopsis. (B) The amino acid sequence alignment between the AtSCD2 and OsCEF3. Different rectangles represent different functional domains.

Additional file 1: Figure S1. The increase rate of frequency by slope from low to high. The increase rate of frequency was defined as the frequency at a specific slope was divided by the accumulated frequency at all observed slopes that were less than the specific slope.

Additional file 7: Figure S7. Schematic representation of the molecular mechanisms underlying IUI-induced lung injury in neonatal mice.

Additional file 5: Fig. S5. The pooled incidences of overall survival at 1, 2, 3, 4, 5 and 10 years after C-ion RT for chondrosarcoma.

Additional file 6: Fig. S6. The pooled incidences of overall survival at 2, 3 and 5 years after C-ion RT for osteosarcoma.

Supplementary Material 1

Supplementary Material 2

Additional file 1: Supplementary Figure 1. Kaplan-Meiercurves of OS differences stratified by age, gender, tumor grade and stagebetween both groups in the entire TCGA set.

Additional file 4: Fig. S2. High-density linkage map consisting of 855 bins was constructed for the RIL population from a cross between Q9311B and WSSM based on the genotypes at 13,847 segregating genes. a, The recombination bin map of the RIL population (n = 1061), in which the horizontal axis indicates the RILs and the vertical axis indicates genomic regions. Q9311B/Q9311B homozygous type is shown in red, WSSM/WSSM homozygous type is shown in blue, and...

Isolation and identification of GSCs. A. a, c: Cells grew into spheres in serum-free medium. b, d: Spheres generated again from a single cell. B. Individual undifferentiated GSCs-87 and GSCs-251 stained for Nestin (green) and CD133 (red) by immunofluorescence analysis. C. Cell spheres were differentiated and then stained for GFAP (green) and β-tubulin III (red) by immunofluorescence analysis. Nuclei (blue) were labeled with DAPI. Images are representative of independent experiments (n = 5). Scale bars...

Supplemental material, sj-tif-3-jet-10.1177_15266028221125581 for Network Meta-Analysis of All Available Regimens Based on Drug-Coated Balloon Angioplasty and Laser Atherectomy for Femoropopliteal In-Stent Restenosis by Chaowen Yu, Wenbo Tang, Tiancai Ren, Qiwei Chen, Ran Lu and Yong Gao in Journal of Endovascular Therapy

Supplemental material, sj-tif-4-jet-10.1177_15266028221125581 for Network Meta-Analysis of All Available Regimens Based on Drug-Coated Balloon Angioplasty and Laser Atherectomy for Femoropopliteal In-Stent Restenosis by Chaowen Yu, Wenbo Tang, Tiancai Ren, Qiwei Chen, Ran Lu and Yong Gao in Journal of Endovascular Therapy