Additional file 4: Figures S3. KEGG pathway enrichment analysis of the 12 differentially expressed proteins.

Additional file 3: Figures S2. GO enrichment analysis of the 12 differentially expressed proteins.

Additional file 2: Figure S2. Raw western blots of GFAP (a), C3d (b), S100a10(c) and β-actin (d) in Figure 4b 12% Gel, 0.45 µm PVDF membrane.

Additional file 5: Figure S5. Raw western blots of GFAP(a), C3d (b), S100a10(c) and β-actin (d) in Figure 7c 12%Gel, 0.45 µm PVDF membrane.

Additional file 2. Original gel of PCR products in the validation experiments of clinical samples with positive chip results for C. albicans.

Additional file 1: Fig. S1. DNA methylation level trends. (A) Bean plots showing the methylation level distribution of methylation sites in the context of CpG, CHG and CHH at genome wide scale. (B) The methylation level trends around gene features per sample. a = promoter (2 kb upstream of transcription start site); b = first exon; c = first intron; d = inner exons; e = inner introns; f = last exon; g = downstream...

Additional file 2: Fig. S2. Methylation status of differentially methylated sites. (A-B) Volcano plots showing DMCs in the context of CHG (A) and CHH (B). (C) Bar plots showing distribution of differences in methylation levels of DMCs.

Additional file 4: Fig. S4. DNA methylation landscape. (A) The number of DMCs grouped by the differences in methylation levels per chromosome. (B-D) Manhattan plots showing the global distribution of DMCs in the context of CpG (B), CHG (C) and CHH (D). (E) DNA methylation landscape of chromosome 2.

Additional file 5: Fig. S5. Effect of cilostazol treatment on cardiac function after CLP. a Representative M-mode images of the four different groups. b-e Quantitative analysis of LVEF (b), LVFS (c), LVEDD (d), and LVESD (e) in each group (n = 6). These data indicate no significant difference.

Additional file 6: Fig. S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...

Additional file 1: Supplementary Figure 1.

Additional file 2: Supplementary Figure 2.

Additional file 3: Supplementary Figure 3.

Additional file 2: Supplementary Figure 2. Partial sensitive compounds.

Additional file 2: Figure S2. Tissue expression of differentially expressed protein-coding genes in atm mutated flies at physiological condition. A Heatmap of differentially expressed genes expressed in larval tissues. B Heatmap of differentially expressed genes expressed in adult female tissues. C Heatmap of differentially expressed genes expressed in adult male tissues.

Additional file 2: Figure S2. Tissue expression of differentially expressed protein-coding genes in atm mutated flies at physiological condition. A Heatmap of differentially expressed genes expressed in larval tissues. B Heatmap of differentially expressed genes expressed in adult female tissues. C Heatmap of differentially expressed genes expressed in adult male tissues.

Supplementary Material 5

Supplementary Material 5

Additional file 3: Figure S3. Quantitative analysis of FTH expression in Fig. 3B.

Additional file 1: Figure S1. Functional enrichment analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). (A) Bubble graph for GO enrichment (the bigger bubble means the more genes enriched, and the increasing depth of red means the differences were more obvious; q-value: the adjusted p-value). (B) KEGG enrichment analysis of differentially expressed genes.

Additional file 5: Figure S5. GSEA enrichment analysis identifies KEGG pathways associated with high-risk and low-risk groups in the training set (A) and test set (B).

Additional file 7: Figure S7. Kaplan-Meier curves of the prognostic pyroptosis-related genes in the training set. The survival curves of NLRP1 (A), NLRP2 (B), GSDMC (C), IL18 (D), CASP4 (E).

Additional file 13. Figure S13. Bubble plots showing the results of DEG functional enrichment analysis using GO gene sets in T cells, NK cells, and cDCs.

Additional file 15. Figure S15. Cross talk between cancer cells and BMDCs. BMDCs were treated with 200 ng/ml LPS or different doses of MSA-2. One day later, the supernatant was discarded, and fresh medium was added. The next day, the conditioned medium from BMDCs was used for CT26 and EMT-6 culture. A day later, cancer cells were harvested for flow cytometry assay. Abs targeting H-2Kd (742436, BD) and PD-L1 (124312, BioLegend) were used in the...

Additional file 15. Figure S15. Cross talk between cancer cells and BMDCs. BMDCs were treated with 200 ng/ml LPS or different doses of MSA-2. One day later, the supernatant was discarded, and fresh medium was added. The next day, the conditioned medium from BMDCs was used for CT26 and EMT-6 culture. A day later, cancer cells were harvested for flow cytometry assay. Abs targeting H-2Kd (742436, BD) and PD-L1 (124312, BioLegend) were used in the...