Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials

Supplemental Material for Fiber reinforced polypropylene composites interfacial behavior improvement fabricated by cold plasma jet SiOx nanoparticles deposition by Lin Zhang, Zhangchuan Xia, Yadong He, Chunling Xin, Yang Yu, Feng Ren and Ruixue Wang in Journal of Composite Materials

Background: Retinal neovascularization, which is characterized by the increased proliferation, migration, and tube formation of retinal microvascular endothelial cells (RMECs), contributes to the progression of diabetic retinopathy (DR). MiR-409-5p has been reported to be upregulated in peripheral blood of DR patients and in vascular endothelial growth factor (VEGF)-induced RMECs. However, the role of miR-409-5p in retinal neovascularization of DR remains unelucidated. Method: The expression of miR-409-5p was measured in retinal tissues of streptozocin-induced and db/db...

Hepatitis E virus (HEV) infection is primarily manifesting as acute hepatitis, but extra-hepatic replication and injury are frequently reported. During the study period, we discovered two acute myeloid leukaemia (AML) patients infected with HEV genotype 3 and 4, respectively, and HEV RNA and/or viral proteins were persistently detected in the bone marrow of both patients. The finding suggests that HEV can replicate in human bone marrow as it may serve as a new target site...

The sodium channel NaV1.5, which is encoded by the SCN5A gene, underlies the fast upstroke of cardiac action potential and thus plays a crucial role in cardiac electrophysiology, but the mechanism governing the regulation of NaV1.5 has not been fully elucidated. The newly developed clustered regularly interspaced short palindromic repeats (CRSPR)/Cas9 transcription factors offer a powerful and precise approach for modulating gene expression. We investigated the potential of this new tool for activating stringently silenced...

Hallucinogenic mushroom is a kind of toxic strain containing psychoactive tryptamine substances such as psilocybin, psilocin and ibotenic acid, etc. The mushrooms containing hallucinogenic components are various, widely distributed and lack of standard to define, which made a great challenge to identification. Traditional identification methods, such as morphology and toxicology analysis, showed shortcomings in old or processed samples, while the DNA-based identification of hallucinogenic mushrooms would allow to identify these samples due to the stability...

Long noncoding RNA muskelin 1 antisense RNA (MKLN1-AS) acted as an oncogenic regulator in hepatocellular carcinoma (HCC). This study was performed to investigate the functional mechanism of MKLN1-AS. MKLN1-AS, microRNA-22-3p (miR-22-3p) and ETS Proto-Oncogene 1 (ETS1) levels were examined using reverse transcription-quantitative polymerase-chain reaction. Protein expression was detected by Western blot. The target relation was analyzed by dual-luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. Cell proliferation ability was determined through cell counting...

Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Triggering receptor expressed on myeloid cells 1 (TREM1) participates in the development of endometritis. This study aims at identifying the effects and interaction of TREM1 and upstream stimulatory factor 2 (USF2) in endometritis by using a model of lipopolysaccharide (LPS)-induced human endometrial epithelial cells (HEnEpCs). ELISA was performed to determine the levels of interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF-α) after LPS stimulation. TREM1 and USF2 expression was examined with RT-qPCR and Western blot....

Ulcerative colitis (UC) is the most prevalent form of chronic inflammatory bowel disease, the etiology of which is poorly understood. This study investigated the role of miR-151-5p on UC and explored the role of brain-derived neurotrophic factor (BDNF) in a UC mouse model and cell model. A UC mouse model was engineered by dextran sulfate sodium (DSS) induction. Primary mouse intestinal epithelial cells (IECs) were isolated. Colitis mice were intraperitoneally injected with miR-151-5p antagomir and...

Supplemental material, sj-jpg-3-tej-10.1177_20417314221147113 for Construction of a pancreatic cancer nerve invasion system using brain and pancreatic cancer organoids by Chenyun Song, Xinyu Chen, Jixin Ma, Hada Buhe, Yang Liu, Hexige Saiyin and Lixiang Ma in Journal of Tissue Engineering

Supplementary figure 1-3 Supplementary table 1-2

Additional file 2: Fig. S2. The pan-cancer landscape of MED12 mutations across human tumors. The proportion of MED12 mutated tumors identified for each cancer type with alteration frequency in TCGA pan-cancer cohorts.

Supplemental material, sj-eps-2-tam-10.1177_17588359221133546 for Circulating tumour DNA biomarkers in savolitinib-treated patients with non-small cell lung cancer harbouring MET exon 14 skipping alterations: a post hoc analysis of a pivotal phase 2 study by Yongfeng Yu, Yongxin Ren, Jian Fang, Lejie Cao, Zongan Liang, Qisen Guo, Sen Han, Zimei Ji, Ye Wang, Yulan Sun, Yuan Chen, Xingya Li, Hua Xu, Jianying Zhou, Liyan Jiang, Ying Cheng, Zhigang Han, Jianhua Shi, Gongyan Chen, Rui Ma, Yun Fan,...

Additional file 9: Fig. S9 Mutational profilesof CSF and plasma samples at baseline and after 8 weeks of treatment.

Additional file 1: Figure S1. The gating strategies for the flow cytometry experiments. Data analysis was performed using FlowJo (Ashland, OR). Scatter and staining with the FITC-anti-CD41 and PE-anti-CD40L antibodies were used to gate platelet population. Cells were first gated by regions within a side scatter area (SSC-A) versus forward scatter area (FSC-A) plot, and then through gating those populations in the SSC-A versus FITC-A plots. Activated platelets were defined as FITC-anti-CD41-A positive and PE-anti-CD40L-A...

Additional file 7: Figure S7. Effect of LPS and CD40L on TRAFs proteins in EGCs. a Representative western blot images of TRAFs proteins expression in EGCs. b–g Western blot analysis of TRAF1 (b), TRAF2 (c), TRAF3 (d), TRAF4 (e), TRAF5 (f) and TRAF6 (g) expression in different groups of EGCs (n = 6). The data are presented as the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Additional file 1: Figure S1. HPLC analysis of SMI from different batches. (A) HPLC analysis of SMI (Lot No. 1907018). (B) HPLC analysis of SMI (Lot No. 1907229). (C) HPLC analysis of SMI (Lot No. 1907277).

Additional file 3: Figure S3. BMNC count in each group. Cells were isolated on Day 10 (n = 6 for Control group, n = 3 for Model group, n = 8 for SMI group, n = 6 for S+Rg group, n = 6 for S group and n = 7 for Rg group). The results are expressed as the means ± SEM. ##p < 0.01, compared with Control group; ＊p < 0.05, ＊＊p < 0.01,...

Supplementary file 1. Figure S1 Flowchart showing the patient inclusion process.

Supplementary file 2. Figure S2 Breast invasive ductal carcinoma. a: Two-dimensional ultrasound image b: Colored blood flow image c: Reliability graph of sound touch elastography, with a reliability of 100% (in the box) d: Sound touch elastography image and elasticity value (in the box) e: Sound touch elastography image and elasticity ratio (in the box) f: strain elastography image and strain ratio (in the box).

Additional file 1: Figure S1. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S1A is related to Fig. 1A; Fig. S1B is related to Fig. 2C; Fig. S1C is related to Fig. 4A.

Additional file 1: Figure S1. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S1A is related to Fig. 1A; Fig. S1B is related to Fig. 2C; Fig. S1C is related to Fig. 4A.

Additional file 3. Collection Proof of Strain GDFS1009.

Additional file 3: Fig. S3. Trends in age standardized incidence, prevalence, death and Dalys rate in 5 SDI regions, From 1990 TO 2019.