888 Works

Long non-coding RNA ZFAS1 regulates cell proliferation and invasion in cervical cancer via the miR-190a-3p/KLF6 axis

Yuehui Su, Wenjing Hou, Chunyan Zhang, Pengcheng Ji, Rui Hu, Qiongying Zhang, Yao Wang, Panpan Li, Huiping Zhang, Yueyue Chen, Xiaodong Zhang & Mengzhen Zhang
Long non-coding RNA (lncRNA) ZFAS1 (zinc finger antisense 1) was demonstrated to play critical roles in various cancer progression. However, the functions of ZFAS in cervical cancers (CC) are unclear. Human CC cell lines were used for in vitro experiments. RT-qPCR (Real Time Quantitative PCR) was performed to detect the expression of ZFAS1, microRNA-190a-3p (miR-190a-3p) and Kruppel-like factor 6 (KLF6). Cell proliferation, invasion and migration assays were used to investigate biological behaviors of CC cells...

Knockdown of lectin-like oxidized low-density lipoprotein-1 ameliorates alcoholic cardiomyopathy via inactivating the p38 mitogen-activated protein kinase pathway

Yifan Zhang, Ruiqi Zhang, Lan Lu, Na Zhou, Xiaoyan Lv, Xin Wang & Zhanbin Feng
LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Melatonin affects hypoxia-inducible factor 1α and ameliorates delayed brain injury following subarachnoid hemorrhage via H19/miR-675/HIF1A/TLR4

Zhijian Xu, Fengduo Zhang, Hu Xu, Fan Yang, Gezhi Zhou, Minfeng Tong, Yaqiang Li & Song Yang
This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Additional file 1 of Novel mechanism of napabucasin, a naturally derived furanonaphthoquinone: apoptosis and autophagy induction in lung cancer cells through direct targeting on Akt/mTOR proteins

Korrakod Petsri, Sunisa Thongsom, Satapat Racha, Supakarn Chamni, Saresa Jindapol, Nantawat Kaekratoke, Hongbin Zou & Pithi Chanvorachote
Additional file 1.

Additional file 4 of Novel mechanism of napabucasin, a naturally derived furanonaphthoquinone: apoptosis and autophagy induction in lung cancer cells through direct targeting on Akt/mTOR proteins

Korrakod Petsri, Sunisa Thongsom, Satapat Racha, Supakarn Chamni, Saresa Jindapol, Nantawat Kaekratoke, Hongbin Zou & Pithi Chanvorachote
Additional file 4.

Effects of penehyclidine hydrochloride on myocardial ischaemia-reperfusion injury in rats by inhibiting TLR4/MyD88/NF-κB pathway via miR-199a-3p

Ling Qiang Bai, Bin Zhe Wang, Qi Wei Liu, Wen Qiang Li, Hang Zhou & Xiao Yan Yang
This study was to probe the role of penehyclidine hydrochloride (PHC) mediating the impact of toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor-kappa B (NF-κB) signalling pathway on myocardial ischaemia-reperfusion injury (MI/RI) in rats through miR-199a-3p. The rat MI/RI model was established through ligating left anterior descending (LAD) coronary artery. PHC was injected preoperatively into the model rats, and injected with miR-199a-3p lentiviral vector or TLR4 antagonist (TAK-242). Next, cardiac function of rats was...

Additional file 5 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 1 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 1 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 1: Figure S1. Toxicity assessment of PMVs@PLGA complexes on cardiac muscle tissue. A TUNEL apoptosis assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. B BAX IF assay of cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats. C BCL-2 IF in cardiomyocytes after targeting the PMVs@PLGA complexes to the heart of SD MIRI model rats (scale bars indicate 100 μm).

Additional file 2 of Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

Tianyi Wang, Tingting Zhou, Mingming Xu, Shuo Wang, Anqi Wu, Mingyang Zhang, You Lang Zhou & Jiahai Shi
Additional file 2: Figure S2. Schematic representation of the sequences of the dual luciferase reporter gene vectors for the 4 miRNAs.

Additional file 4 of Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Yanchun Li, Bing Xu, Xueying Ren, Luyang Wang, Yaqing Xu, Yefeng Zhao, Chen Yang, Chen Yuan, Huanjuan Li, Xiangmin Tong, Ying Wang & Jing Du
Additional file 4: Figure S4. (A) Analysis of NQO1 expression by RT-PCR in CISD2 silenced HT-1080 cells with or without the treatment of erastin; (B) Quantitative analysis of NQO1 expression in Fig. 4A;★P < 0.05 and ★★P < 0.01 between the indicated groups.

Additional file 12 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 12: Figure S6. Widespread occurrence of rumen bacteria in the communication network. (A) The occurrence of rumen bacteria in the network. (B) The occurrence of bacteria that synthesize AI-2 at the phylum level. (C) The occurrence of bacteria that synthesize and sense AI-2 at the phylum level. (D) The occurrence of bacteria that sense AI-2 at the phylum level. Numbers indicate the numbers of bacterial genomes in which the corresponding functions were found...

Additional file 12 of Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

Ming Yi, Mengke Niu, Yuze Wu, Hong Ge, Dechao Jiao, Shuangli Zhu, Jing Zhang, Yongxiang Yan, Pengfei Zhou, Qian Chu & Kongming Wu
Additional file 12. Figure S12. Histogram representing the proportion of clusters in each group.

Additional file 13 of Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

Ming Yi, Mengke Niu, Yuze Wu, Hong Ge, Dechao Jiao, Shuangli Zhu, Jing Zhang, Yongxiang Yan, Pengfei Zhou, Qian Chu & Kongming Wu
Additional file 13. Figure S13. Bubble plots showing the results of DEG functional enrichment analysis using GO gene sets in T cells, NK cells, and cDCs.

Additional file 14 of Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

Ming Yi, Mengke Niu, Yuze Wu, Hong Ge, Dechao Jiao, Shuangli Zhu, Jing Zhang, Yongxiang Yan, Pengfei Zhou, Qian Chu & Kongming Wu
Additional file 14. Figure S14. Reclustering analysis of tumor-infiltrating NK cells. (a) T-distributed stochastic neighbor embedding (t-SNE) plot for NK cells. (b) t-SNE plot for the treatment group-specific distribution of NK cell subclusters. (c) Violin plots showing NK cell subcluster-specific gene profiles. (d) Histogram representing the proportion of NK cells subcluster in each group. (e-f) Violin plots showing the levels of Cd69 and Tnf of NK cell subclusters. (g) Heatmap depicting the results of GSEA...

Additional file 4 of Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

Ming Yi, Mengke Niu, Yuze Wu, Hong Ge, Dechao Jiao, Shuangli Zhu, Jing Zhang, Yongxiang Yan, Pengfei Zhou, Qian Chu & Kongming Wu
Additional file 4. Figure S4: The effects of MSA-2 and TGF-β on chemokine production in BMDM. (a-f) Unactivated BMDMs were cultured with MSA-2 for one day, and supernatants were collected for chemokine detection.

Additional file 8 of Costunolide is a dual inhibitor of MEK1 and AKT1/2 that overcomes osimertinib resistance in lung cancer

Xueli Tian, Rui Wang, Tingxuan Gu, Fayang Ma, Kyle Vaughn Laster, Xiang Li, Kangdong Liu, Mee-Hyun Lee & Zigang Dong
Supplementary Material 8

Additional file 1 of B7-H4 is a potential diagnostic and prognostic biomarker in colorectal cancer and correlates with the epithelial-mesenchymal transition

Xiaotian Yan, Bo Hong, Jie Feng, Yuanqing Jin, Mengting Chen, Fugang Li & Yun Qian
Additional file 1: Fig. S1. Paired IHC staining of B7-H4 in whole tissue sections and corresponding tissue microarrays of tumor tissues. (A-B)Both high expression in tumor cells; (C-D) Both low expression in tumor stroma; (E-F) Both moderate expression in tumor stroma; (G-H) Both low expression in tumor cells; (I-J) Both high expression in tumor cells and moderate expression in tumor stroma; (K-L)Both high expression in tumor cells; (M-N) Both high expression in tumor cells; (O-P)...

Additional file 1 of The optimal neoadjuvant chemotherapy regimen for locally advanced gastric and gastroesophageal junction adenocarcinoma: a systematic review and Bayesian network meta-analysis

Tongya Wang, Changyang Li, Xiang Li, Jing Zhai, Shoulin Wang & Lizong Shen
Additional file 1: Figure S1. Systematic review of NAC-related grade 3/4 adverse events and postoperative 30-day mortality. (A) The frequency of grade 3/4 adverse events and postoperative 30-day mortality in each regimen. (B) Forest plots of comparisons for grade 3/4 adverse events and postoperative 30-day mortality (S, surgery).

Additional file 2 of Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Mengqi Wang, Nathalie Bissonnette, Mario Laterrière, Pier-Luc Dudemaine, David Gagné, Jean-Philippe Roy, Xin Zhao, Marc-André Sirard & Eveline M. Ibeagha-Awemu
Additional file 2: Fig. S2. Methylation status of differentially methylated sites. (A-B) Volcano plots showing DMCs in the context of CHG (A) and CHH (B). (C) Bar plots showing distribution of differences in methylation levels of DMCs.

Additional file 1 of Genome-wide screening for differentially methylated long noncoding RNAs identifies LIFR-AS1 as an epigenetically regulated lncRNA that inhibits the progression of colorectal cancer

Peng Song, Ying Li, Feng Wang, Lingxiao Pu, Linsen Bao, Hengfei Gao, Chuandong Zhu, Meng Wang & Liang Tao
Additional file 1: Figure S1. Pie chart shows the number of differentially expressed lncRNAs in each category.

Additional file 4 of Genome-wide screening for differentially methylated long noncoding RNAs identifies LIFR-AS1 as an epigenetically regulated lncRNA that inhibits the progression of colorectal cancer

Peng Song, Ying Li, Feng Wang, Lingxiao Pu, Linsen Bao, Hengfei Gao, Chuandong Zhu, Meng Wang & Liang Tao
Additional file 4: Figure S3. Volcano plot showing the log2 fold change of 517 significantly differentially expressed miRNAs in CRC patients from the TCGA database.

Additional file 1 of CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma

Jungang Zhao, Rizhao Li, Jiacheng Li, Ziyan Chen, Zixia Lin, Baofu Zhang, Liming Deng, Gang Chen & Yi Wang
Supplementary Material 1

Additional file 2 of CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma

Jungang Zhao, Rizhao Li, Jiacheng Li, Ziyan Chen, Zixia Lin, Baofu Zhang, Liming Deng, Gang Chen & Yi Wang
Supplementary Material 2

Additional file 3 of CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma

Jungang Zhao, Rizhao Li, Jiacheng Li, Ziyan Chen, Zixia Lin, Baofu Zhang, Liming Deng, Gang Chen & Yi Wang
Supplementary Material 3

Registration Year

  • 2022
    888

Resource Types

  • Image
    888

Affiliations

  • Zhejiang University
    888
  • Fudan University
    563
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    549
  • Shanghai Jiao Tong University
    510
  • Capital Medical University
    496
  • Sichuan University
    479
  • Sun Yat-sen University
    466
  • Nanjing Medical University
    462
  • Huazhong University of Science and Technology
    453
  • Chinese Academy of Sciences
    434