Gastric cancer (GC) still poses a significant threat to human life. Hence, there is an urgent need to understand the mechanism of GC progression and develop novel therapeutics approach to treating GC. This study was conducted to evaluate the role of the lncRNA SNHG22 in the progression of GC. First, GC data from TCGA were analyzed using GEPIA. After the starbase database was used to predict SNHG22 target miRNA and miR-101-3p target mRNA. The predictions...

Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2 expression of each sample determined by RT-qPCR. In addition, as miR-34a has a...

Ulcerative colitis (UC) is the most prevalent form of chronic inflammatory bowel disease, the etiology of which is poorly understood. This study investigated the role of miR-151-5p on UC and explored the role of brain-derived neurotrophic factor (BDNF) in a UC mouse model and cell model. A UC mouse model was engineered by dextran sulfate sodium (DSS) induction. Primary mouse intestinal epithelial cells (IECs) were isolated. Colitis mice were intraperitoneally injected with miR-151-5p antagomir and...

Additional file 11: Figure S5. Expression of the predicted AI-2 synthases and receptors within a rumen microbial metatranscriptome at the phylum level. (A) The number of bacterial genomes containing AI-2-related genes and the percentage of corresponding genomes that expressed these genes. (B) The number of bacterial genomes containing luxS synthases and the percentage of the corresponding genomes that expressed luxS genes at the phylum level. (C) The number of bacterial genomes containing lsrB receptors and...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 21: Fig. S8. Distribution of sequencing reads that mapped to the promoter region (upstream 2,000 bp) of the PbTIC55 gene from 113 pear accessions derived from four populations (wild Asian, cultivated Asian, wild European, and cultivated European populations). The results of this analysis did not identify any potential indels or SVs in the promoter region of TIC55 between the Asian and European pear populations.

Additional file 21: Fig. S8. Distribution of sequencing reads that mapped to the promoter region (upstream 2,000 bp) of the PbTIC55 gene from 113 pear accessions derived from four populations (wild Asian, cultivated Asian, wild European, and cultivated European populations). The results of this analysis did not identify any potential indels or SVs in the promoter region of TIC55 between the Asian and European pear populations.

Additional file 6: Figure S2. Distribution and abundance of AI-2 synthases and receptors based on QS in the rumen bacterial genomes. Numbers indicate the number of bacterial genomes in which the corresponding proteins were found at the genus level. The LuxS synthases, LsrB receptors, and CahR-type receptors were discovered within 88, 27, and 42 genera, respectively.

Additional file 9: Figure S4. The types of the CahR-type receptors. Numbers indicate the number of bacterial genomes in which the corresponding proteins were found. The MCPs and HKs were two dominant AI-2 receptor proteins in rumen bacteria.

Supplementary Material 2

Additional file 2: Figure S2. Genomic alterations of metabolic subtypes in the TCGA-LIHC cohort.

Additional file 3: Figure S3. ShRNA Panx 1-AAV or Ctrl-AAV-labeled EGFP-positive cells have a sound co-localization with S100 β-labeled Schwann cells. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 50 μm. Lower panel, scale bar = 25 μm. n = 3–4 mice/group.

Additional file 5: Figure S5. The effect of Panx 1-siRNA on the expression of Panx 1 in Schwann cells. A, D, and E. mRNA expression of Panx 1, Panx 2, and Panx 3 after treatment with Panx 1 siRNA. B-C. Gel images and quantification of Panx 1 protein levels after treatment with Panx 1 siRNA. F. Panx 1-specific siRNA decreased Panx 1 mRNA expression levels under LPS treatment in Schwann cells. All data are mean...

Additional file 6: Figure S6. CBX and Panx 1-siRNA do not affect TGF-β mRNA expression in LPS-treated Schwann cells. A-B. Pretreatment with CBX (100 mM) for 2 h or Panx 1 siRNA for 36 h, qPCR was used to determine TGF-β mRNA expression levels in LPS-treated Schwann cells. All data are mean ± SEM. n = 4.

Supplementary Material 2

Additional file 6.

Supplemental material, sj-tif-2-jet-10.1177_15266028221125581 for Network Meta-Analysis of All Available Regimens Based on Drug-Coated Balloon Angioplasty and Laser Atherectomy for Femoropopliteal In-Stent Restenosis by Chaowen Yu, Wenbo Tang, Tiancai Ren, Qiwei Chen, Ran Lu and Yong Gao in Journal of Endovascular Therapy

Supplemental material, sj-tif-4-jet-10.1177_15266028221125581 for Network Meta-Analysis of All Available Regimens Based on Drug-Coated Balloon Angioplasty and Laser Atherectomy for Femoropopliteal In-Stent Restenosis by Chaowen Yu, Wenbo Tang, Tiancai Ren, Qiwei Chen, Ran Lu and Yong Gao in Journal of Endovascular Therapy

Additional file 5: Figure S5. The effect of Panx 1-siRNA on the expression of Panx 1 in Schwann cells. A, D, and E. mRNA expression of Panx 1, Panx 2, and Panx 3 after treatment with Panx 1 siRNA. B-C. Gel images and quantification of Panx 1 protein levels after treatment with Panx 1 siRNA. F. Panx 1-specific siRNA decreased Panx 1 mRNA expression levels under LPS treatment in Schwann cells. All data are mean...

Supplementary Material 3

Supplementary Material 5

Supplementary Material 8

Additional file 10: Figure S10. A.443654 inhibits the cell proliferation of PAAD. (A) Cell viability of PANC-1 cell line treated with DMSO and 50 nM A.443654, respectively. *P < 0.05, **P < 0.01 by a two-tailed unpaired t-test. (B) Colony formation assay of PANC-1 cells treated with DMSO and 50 nM A.443654, respectively. **P < 0.01 by a two-tailed unpaired t-test. (C) Edu assay of PANC-1 cells treated with DMSO and 50 nM A.443654, respectively....

Additional file 1: Figure S1. Functional enrichment analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). (A) Bubble graph for GO enrichment (the bigger bubble means the more genes enriched, and the increasing depth of red means the differences were more obvious; q-value: the adjusted p-value). (B) KEGG enrichment analysis of differentially expressed genes.