Additional file 3: Fig S2. Origin and evolution of LSSL. (A) The phylogenetic tree for the LSSL family based on the neighbor-joining (NJ) method. An NJ tree was constructed using the amino acid sequences of the LSSL proteins. All species represented by abbreviations are shown in Table S2. The bar (0.020) indicates the genetic distance. Different color lines correspond to different species groups. (B) The motif composition of the LSSL family proteins. (C) Model diagram...

Additional file 5: Fig S4. Glycan selectivity of LSSL assessed by glycan microarrays. (A) LSSL (8 µg/mL) binding to 100 glycan microarray. The concentrations given for the glycan microarray represent those used in the carbohydrate immobilization reaction. Data are presented as the mean ± SDs; (n = 3 technical replicates). (B) Confocal microscopy detection of LSSL binding to yeast. LSSL were incubated with yeast cells (105 cells/well) in PBS for 1 h at room temperature...

Additional file 2: Fig. S1. A Schematic diagram of the genomic locus of CEBPA-DT (www.ncbi.nlm.nih.gov/). B The protein-coding potential of CEBPA-DT predicted by Open reading frame (ORF) Finder software prediction ( https://www.ncbi.nlm.nih.gov/orffinder/ ). C The protein-coding potential of CEBPA-DT predicted by Coding-Potential Assessment Tool ( wlcb.oit.uci.edu/cpat/ ).

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 6: Fig. S5. A-B The expression levels of DDR2 were measured by western blot and RT-qPCR in indicated HCCLM9 and SNU-449 cells transfected with negative control siRNA or siRNAs targeting DDR2. C-D Cell migration capacity of indicated HCCLM9 and SNU-449 cells were measured by scratch wound-healing assays. Scale bar, 100μm. E-F Cell migration and invasion capacity of indicated HCCLM9 and SNU-449 cells were measured by transwell migration and matrigel invasion assays. Scale bar,...

Autophagy plays an important role in defending against invading microbes. However, numerous viruses can subvert autophagy to benefit their replication. Porcine epidemic diarrhoea virus (PEDV) is an aetiological agent that causes severe porcine epidemic diarrhoea. How PEDV infection regulates autophagy and its role in PEDV replication are inadequately understood. Herein, we report that PEDV induced complete autophagy in Vero and IPEC-DQ cells, as evidenced by increased LC3 lipidation, p62 degradation, and the formation of autolysosomes....

Additional file 1: Fig. 1. (A) Representative immunofluorescence images showing DNA damage in control, RRP-9-overexpressing or RRP-9 silenced pancreatic cells after gemcitabine treatment. Green, γ-H2AX; blue, nuclei. Scale bar, 10 μm. (B) Western blot analysis of DNA damage (γ-H2AX) and apoptosis (cleaved caspase-3 and cleaved PARP) markers in control, RRP-9-overexpressing or RRP-9 silenced PC cells after gemcitabine treatment (50 μM). GAPDH acted as the loading control

Additional file 1: Fig. 1. (A) Representative immunofluorescence images showing DNA damage in control, RRP-9-overexpressing or RRP-9 silenced pancreatic cells after gemcitabine treatment. Green, γ-H2AX; blue, nuclei. Scale bar, 10 μm. (B) Western blot analysis of DNA damage (γ-H2AX) and apoptosis (cleaved caspase-3 and cleaved PARP) markers in control, RRP-9-overexpressing or RRP-9 silenced PC cells after gemcitabine treatment (50 μM). GAPDH acted as the loading control

Additional file 1: Fig. S1. Circ_0017109 promotes cell growth and inhibits apoptosis of NSCLC cells. (A) Circ_0017109 expression within H1299 and A549 cells after the transfection of si-circ_0017109#2 or si-NC. (B) CCK-8 assay in H1299 and A549 cells after the transfection of si-circ_0017109#2 or si-NC. (C) Apoptosis detection in H1299 and A549 cells after the transfection of si-circ_0017109 or si-NC. **, P < 0.01, and ***, P < 0.001.

Additional file 5. Collection Proof of Strain RW10569.

Supplementary Material 3

Supplemental material, sj-tif-2-cll-10.1177_09636897221139103 for Low-Dose Anti-Thymocyte Globulin Plus Low-Dose Posttransplant Cyclophosphamide as an Effective Regimen for Prophylaxis of Graft Versus Host Disease After Haploidentical Peripheral Blood Stem Cell Transplantation With Maternal/Collateral Related Donors by Ting Li, Qiaomei He, Jun Yang, Yu Cai, Chongmei Huang, Xiaowei Xu, Huiying Qiu, Jiahua Niu, Kun Zhou, Yin Zhang, Xinxin Xia, Yu Wei, Chang Shen, Xueying Ding, Yin Tong, Liping Wan and Xianmin Song in Cell Transplantation

Supplemental material, sj-tif-3-cll-10.1177_09636897221139103 for Low-Dose Anti-Thymocyte Globulin Plus Low-Dose Posttransplant Cyclophosphamide as an Effective Regimen for Prophylaxis of Graft Versus Host Disease After Haploidentical Peripheral Blood Stem Cell Transplantation With Maternal/Collateral Related Donors by Ting Li, Qiaomei He, Jun Yang, Yu Cai, Chongmei Huang, Xiaowei Xu, Huiying Qiu, Jiahua Niu, Kun Zhou, Yin Zhang, Xinxin Xia, Yu Wei, Chang Shen, Xueying Ding, Yin Tong, Liping Wan and Xianmin Song in Cell Transplantation

Supplemental material, sj-tif-2-jbm-10.1177_03936155221140421 for Diagnostic and prognostic values of MMP-9 expression in ovarian cancer: A study based on bioinformatics analysis and meta-analysis by Changyu Liu, Ying Shen and Qiyan Tan in The International Journal of Biological Markers

Additional file 5: Figure sup 5. Transcriptional changes of selected immuneresponse-related genes in apoE-TR mice upon cuprizone-induced demyelination.RNA was extracted from the CC area of CTL (n=5/genotype) and CPZ-treated (n=5-6/genotype) apoE-TR mice. (A-F)The expression of immune response-associated genes (i.e., Cst7, Ccl2, Csf1, Csf1r, Serpina3n, and Tnf-α) wasmeasured by real-time PCR. Values are mean ± SEM. Mann-Whitney tests followedby Bonferroni correction for multiple comparisons were used. *P < 0.0167;N.S. not significant.

Additional file 4: Figure S4. WB raw data of Figure 4B.

Additional file 8: Fig. S8. Mapchart shown the distribution of GRAS genes on 7 chromosomes in black raspberry.

Additional file 3: Fig. S3. Mapchart shown the distribution of GRAS genes on 7 chromosomes in woodland strawberry.

Additional file 4: Fig. S4. Mapchart shown the distribution of GRAS genes on 17 chromosomes in apple.

Supplemental material, sj-png-2-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Additional file 1.

Additional file 3.

Additional file 3: Figure S3. Significantly regulated PI3K pathway genes in HeLa cells 2 h after 4 Gy irradiation compared to unirradiated cells

Additional file 2: Figure S2. A Scatter plot showing RNA expression changes of genes in HeLa cells 0.5 h after irradiation compared to unirradiated cells. KEGG B and GO C analyses of differentially expressed genes in HeLa cells 0.5 h after irradiation compared to unirradiated cells

Additional file 2: Figure S2. Validation of the pyroptosis-related prognostic model in test set. (A) The patients were divided into two groups according to the threshold of median risk score. Green represents the low-risk group. Red represents the high-risk group. (B) Kaplan Meier curves showing the overall survival of patients in the high-risk and low-risk groups. (C) Survival status of patients with PAAD in high and low risk groups. Green represents survival. Red represents death....