114 Works

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

Additional file 1 of Neuronal nuclear calcium signaling suppression of microglial reactivity is mediated by osteoprotegerin after traumatic brain injury

Albrecht Fröhlich, Florian Olde Heuvel, Rida Rehman, Sruthi Sankari Krishnamurthy, Shun Li, Zhenghui Li, David Bayer, Alison Conquest, Anna M. Hagenston, Albert Ludolph, Markus Huber-Lang, Tobias Boeckers, Bernd Knöll, Maria Cristina Morganti-Kossmann, Hilmar Bading & Francesco Roselli
Additional file 1: Figure S1. Glial CREB phosphorylation post injury is limited to IBA1+ cells. A)-B) AAVs are able to infect 89.98 ± 14.47% of cortical neurons. Data are shown as mean ± SD (Red dots represent individual animals). N=8. Scale bar: 50µm. C)-D) Non-neuronal pCREB signal is mainly found in IBA1+ cells (IBA1+ cells: 97.76 ± 0.83% vs. GFAP+ cells: 1.24 ± 0.83%). Data are shown as mean ± SD. N=4. Scale Bar: 50µm.E-F)...

Additional file 3 of Neuronal nuclear calcium signaling suppression of microglial reactivity is mediated by osteoprotegerin after traumatic brain injury

Albrecht Fröhlich, Florian Olde Heuvel, Rida Rehman, Sruthi Sankari Krishnamurthy, Shun Li, Zhenghui Li, David Bayer, Alison Conquest, Anna M. Hagenston, Albert Ludolph, Markus Huber-Lang, Tobias Boeckers, Bernd Knöll, Maria Cristina Morganti-Kossmann, Hilmar Bading & Francesco Roselli
Additional file 3: Figure S3. Blunting neuronal nuclear calcium signaling did not alter amplitude and velocity of whisker movement post-TBI. A)-C) The amplitudes of the affected (CS = 0.88 ± 0.22; CT = 1.1 ± 0.19; PS = 0.93 ± 0.42; PT = 1.269 ± 0.56) or unaffected (CS = 1.07 ± 0.39; CT = 1.05 ± 0.35; PS = 1.14 ± 0.45; PT = 1.22 ± 0.45) whisker were not significantly changed 24h after...

Additional file 4 of Neuronal nuclear calcium signaling suppression of microglial reactivity is mediated by osteoprotegerin after traumatic brain injury

Albrecht Fröhlich, Florian Olde Heuvel, Rida Rehman, Sruthi Sankari Krishnamurthy, Shun Li, Zhenghui Li, David Bayer, Alison Conquest, Anna M. Hagenston, Albert Ludolph, Markus Huber-Lang, Tobias Boeckers, Bernd Knöll, Maria Cristina Morganti-Kossmann, Hilmar Bading & Francesco Roselli
Additional file 4: Figure S4. Neuronal expression of osteoprotegerin is upregulated by nuclear calcium signaling and neuronal activity in TBI. A) Negative Control, baseline OPG (TNFRSF11B) signal and a positive Control of the in situ hybridization 24h post injury. B) OPG (TNFRSF11B) in situ signal is mainly found in neuronal sources (neuronal vs non-neuronal 75.38 ± 1.83% vs 24.62% ± 1.83%). N=5. C)-D) Significant increase of OPG (TNFRSF11b) mRNA density 24h post-TBI compared to sham...

Additional file 3 of Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Additional file 3: Fig. S1. Phenotypic distribution for 12 traits at the three environments. a, In recombined inbred lines (RILs). b, In backcross F1s hybrids (BCF1s). c, Mid-parental heterosis values. d, Over-standard heterosis values. Over-standard heterosis was calculated by [(BCF1 – QYSM)/QYSM] × 100. RILs derived from a cross between Q9311B and WSSM, BCF1s were hybrids between the RILs and Q9311A. HD, heading date; PH, plant height; PN, panicle number per plant; FLL, flag leaf...

Additional file 4 of Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Additional file 4: Fig. S2. High-density linkage map consisting of 855 bins was constructed for the RIL population from a cross between Q9311B and WSSM based on the genotypes at 13,847 segregating genes. a, The recombination bin map of the RIL population (n = 1061), in which the horizontal axis indicates the RILs and the vertical axis indicates genomic regions. Q9311B/Q9311B homozygous type is shown in red, WSSM/WSSM homozygous type is shown in blue, and...

Additional file 5 of Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations

Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen & Jianlong Xu
Additional file 5: Fig. S3. Genomic distribution of main-effect QTLs affecting 12 rice yield related traits identified in the RIL and BCF1 populations separately.

Additional file 4 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 4: Figure S4. The responses of LPS-treated cultured Schwann cells. A-E. mRNA expression levels of the inflammatory factors, IL-1β, IL-6, TNF-α, and TGF-β (A-D), the apoptosis marker, caspase 3 (E) and in LPS-treated Schwann cells (0, 100, 500, and 1000 ng/ml, 24 h at 37℃). F. Cytomorphology of LPS-treated Schwann cells at different doses (0, 100, 500, and 1000 ng/ml) under a light microscope. All data are mean ± SEM. n = 3....

Additional file 6 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 6: Figure S6. CBX and Panx 1-siRNA do not affect TGF-β mRNA expression in LPS-treated Schwann cells. A-B. Pretreatment with CBX (100 mM) for 2 h or Panx 1 siRNA for 36 h, qPCR was used to determine TGF-β mRNA expression levels in LPS-treated Schwann cells. All data are mean ± SEM. n = 4.

Additional file 1 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 1: Figure S1. Panx 2 and Panx 3 mRNA expression after CCI. A-B. A qPCR assay was used to detect Panx 2 (A) and Panx 3 (B) mRNA levels at 4 and 14 days post-CCI. The data are mean ± SEM. n = 3 mice/group. **p < 0.01, ***p < 0.001, vs. sham injury. The data were analyzed by one-way ANOVA followed by Dunnett’s test.

Additional file 2 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 2: Figure S2. The distribution of Panx 1 in the DRG and within sciatic nerves. A. Double-immunofluorescence images of Panx 1 (green) and the sciatic nerve axonal marker, NF200 (red). (B). Images of Panx 1 immunostaining (red) and, Nissl staining (green) in the DRG; Scale bar = 50 μm. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 100 μm. Lower panel, scale bar...

Additional file 1 of Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Mengqi Wang, Nathalie Bissonnette, Mario Laterrière, Pier-Luc Dudemaine, David Gagné, Jean-Philippe Roy, Xin Zhao, Marc-André Sirard & Eveline M. Ibeagha-Awemu
Additional file 1: Fig. S1. DNA methylation level trends. (A) Bean plots showing the methylation level distribution of methylation sites in the context of CpG, CHG and CHH at genome wide scale. (B) The methylation level trends around gene features per sample. a = promoter (2 kb upstream of transcription start site); b = first exon; c = first intron; d = inner exons; e = inner introns; f = last exon; g = downstream...

Additional file 2 of Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Mengqi Wang, Nathalie Bissonnette, Mario Laterrière, Pier-Luc Dudemaine, David Gagné, Jean-Philippe Roy, Xin Zhao, Marc-André Sirard & Eveline M. Ibeagha-Awemu
Additional file 2: Fig. S2. Methylation status of differentially methylated sites. (A-B) Volcano plots showing DMCs in the context of CHG (A) and CHH (B). (C) Bar plots showing distribution of differences in methylation levels of DMCs.

Additional file 4 of Methylome and transcriptome data integration reveals potential roles of DNA methylation and candidate biomarkers of cow Streptococcus uberis subclinical mastitis

Mengqi Wang, Nathalie Bissonnette, Mario Laterrière, Pier-Luc Dudemaine, David Gagné, Jean-Philippe Roy, Xin Zhao, Marc-André Sirard & Eveline M. Ibeagha-Awemu
Additional file 4: Fig. S4. DNA methylation landscape. (A) The number of DMCs grouped by the differences in methylation levels per chromosome. (B-D) Manhattan plots showing the global distribution of DMCs in the context of CpG (B), CHG (C) and CHH (D). (E) DNA methylation landscape of chromosome 2.

Additional file 2 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 2: Figure S2. Genes expression levels in lung tissues of neonatal mice (A) The mRNA expression of differentially expressed genes analysed using RT-qPCR in lung tissues of neonatal mice. B, C Nr4a1 expression analysed using western blot in lung tissues of neonatal mice. D Serpine1 expression in lung tissues of neonatal mice detected using RT-qPCR on P1, P7, and P21. E, F Serpine1 expression analysed using RT-qPCR and western blot in siRNA-treated neonatal...

Additional file 7 of miR-146a-5p-modified hUCMSC-derived exosomes facilitate spinal cord function recovery by targeting neurotoxic astrocytes

Xunwei Lai, Yang Wang, Xiaokang wang, Bin Liu & Limin Rong
Additional file 7. A Successful delivery of PKH-26 labeled exosomes to the spinal cord lesion. Scale bar = 50 nm. B The successful delivery of miR-146a-5p to the spinal cord lesion.

Additional file 9 of miR-146a-5p-modified hUCMSC-derived exosomes facilitate spinal cord function recovery by targeting neurotoxic astrocytes

Xunwei Lai, Yang Wang, Xiaokang wang, Bin Liu & Limin Rong
Additional file 9. Detailed region where the representative images of Figure 2A selected from. LC, lesion cord.

Additional file 3 of LncRNA CEBPA-DT promotes liver cancer metastasis through DDR2/β-catenin activation via interacting with hnRNPC

Yunshi Cai, Tao Lyu, Hui Li, Chang Liu, Kunlin Xie, Lin Xu, Wei Li, Hu Liu, Jiang Zhu, Yinghao Lyu, Xuping Feng, Tian Lan, Jiayin Yang & Hong Wu
Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 8 of LncRNA CEBPA-DT promotes liver cancer metastasis through DDR2/β-catenin activation via interacting with hnRNPC

Yunshi Cai, Tao Lyu, Hui Li, Chang Liu, Kunlin Xie, Lin Xu, Wei Li, Hu Liu, Jiang Zhu, Yinghao Lyu, Xuping Feng, Tian Lan, Jiayin Yang & Hong Wu
Additional file 8: Fig. S7. A-B Subgroup analyses showed prognostic value of CEBPA-DT for overall survival (top) and recurrence-free survival (bottom) in HCC patients with differential features.

Additional file 8 of LncRNA CEBPA-DT promotes liver cancer metastasis through DDR2/β-catenin activation via interacting with hnRNPC

Yunshi Cai, Tao Lyu, Hui Li, Chang Liu, Kunlin Xie, Lin Xu, Wei Li, Hu Liu, Jiang Zhu, Yinghao Lyu, Xuping Feng, Tian Lan, Jiayin Yang & Hong Wu
Additional file 8: Fig. S7. A-B Subgroup analyses showed prognostic value of CEBPA-DT for overall survival (top) and recurrence-free survival (bottom) in HCC patients with differential features.

High perirenal fat thickness predicts a greater risk of recurrence in Chinese patients with unilateral nephrolithiasis

Haichao Huang, Shi Chen, Wenzhao Zhang, Tao Wang, Peide Bai, Jinchun Xing, Huiqiang Wang & Bin Chen
The aim of this study was to evaluate the association between recurrence-free survival (RFS) and perirenal fat thickness (PFT) in a cohort of Chinese population with unilateral nephrolithiasis. We retrospectively reviewed the medical records of 81 patients with unilateral nephrolithiasis in our center from January 2019 to June 2019. PFT measured on computed tomography (CT) scans was evaluated. Kaplan-Meier curves and log-rank tests were used to assess significant differences in RSF between high-PFT and low-PFT...

WD repeat domain 62 (WDR62) promotes resistance of colorectal cancer to oxaliplatin through modulating mitogen-activated protein kinase (MAPK) signaling

Juanjuan Cai, Lingling Su & Weiwei Luo
WD repeat domain 62 (WDR62) is involved in embryonic brain growth through regulation of glial and neural cell populations. WDR62 is also implicated in the carcinogenesis of various cancers. The role of WDR62 in progression and chemoresistance of colorectal cancer (CRC) was investigated. Firstly, oxaliplatin-resistant CRC cells (HCT116/R and HT29/R) were sequentially exposed to an increasing concentration of oxaliplatin. The results showed that WDR62 was elevated in CRC tissues, and oxaliplatin resistance conferred up-regulation of...

Additional file 5 of Genetic diversity in the transmission-blocking vaccine candidate Plasmodium vivax gametocyte protein Pvs230 from the China–Myanmar border area and central Myanmar

Xin Zhao, Yubing Hu, Yan Zhao, Lin Wang, Zifang Wu, Myat Thu Soe, Myat Phone Kyaw, Liwang Cui, Xiaotong Zhu & Yaming Cao
Additional file 5: Figure S2. Median-joining network of Pv230 among eight geographically diverse populations. Haplotypes composed of nucleotide polymorphism in ICP of Pv230 with a frequency > 1 were used to create a median-joining network. Each node represents one haplotype, node size indicates haplotype frequency, and node color corresponds to the country of origin. Line length is proportional to genetic distance.

Additional file 3 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 3: Figure S3. ShRNA Panx 1-AAV or Ctrl-AAV-labeled EGFP-positive cells have a sound co-localization with S100 β-labeled Schwann cells. Enlarged images in lower panel are from the inset boxes of upper panel. Upper panel, scale bar = 50 μm. Lower panel, scale bar = 25 μm. n = 3–4 mice/group.

Additional file 7 of Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses

Qian Wang, Han-yang Li, Zhuo-min Ling, Gang Chen & Zhong-Ya Wei
Additional file 7: Figure S7. Cytokine array reveals Panx 1-dependent cytokines and chemokines in LPS-treated Schwann cells. A. Coordinates of the cytokine array containing 40 different cytokines and chemokines, including three positive control (PC) proteins. B. Array membranes of protein expression among Ctrl, Ctrl-siRNA, and Panx 1-siRNA groups in LPS-treated Schwann cells.

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  • Institute of Hydrobiology
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  • University of Chinese Academy of Sciences
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  • Chinese Academy of Medical Sciences & Peking Union Medical College
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  • Shanghai Jiao Tong University
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  • Shandong University
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  • Nanjing Medical University
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  • Ministry of Education of the People's Republic of China
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