Psoriasis is a chronic inflammatory skin disease that affects approximately 2% of worldwide population, and causing long-term troubles to the patients. Therefore, it is urgent to develop safe and effective therapeutic drugs. Catalpol is a natural iridoid glucoside, that has several remarkable pharmacological effects, however, whether catalpol can alleviated psoriasis has not been explored. The goal of the present work is to study the role of catalpol in psoriasis in vivo and in vitro. Imiquimod-induced...

Additional file 4: Figure S2. Hazard ratios for long-term all-cause mortality in different subgroups.

To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Additional file 2: Figure S2. Results of western blot analysis showed that ETV2 overexpression induced the the activation of p-ERK and p-AKT at days 3, 7 and 14 of osteogenic differentiation. *P < 0.05, compared with the 0 day group.

Additional file 3: Figure S3. HE (scale bar = 200 μm) and Masson (scale bar = 50 μm) staining in the rat calvarial defect model (n = 6).

Additional file 1. AKG treatment in mice with the sham surgery did not affect abdominal aortic diameter.

Additional file 2. Original gel of PCR products in the validation experiments of clinical samples with positive chip results for C. albicans.

Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 8: Fig. S7. A-B Subgroup analyses showed prognostic value of CEBPA-DT for overall survival (top) and recurrence-free survival (bottom) in HCC patients with differential features.

Additional file 8: Fig. S7. A-B Subgroup analyses showed prognostic value of CEBPA-DT for overall survival (top) and recurrence-free survival (bottom) in HCC patients with differential features.

Additional file 2: Fig. S2. Correlation between biventricular global strain and age assessed by Medis. Linear regressions with the 95% prediction band for LV global radial strain (GRS) (a), global circumferential strain (GCS) (b), global longitudinal strain (GLS) (c), and RV free wall- global longitudinal strain (RVFW-GLS) (d).

Additional file 3. PXDN was overexpressed in aorta by the injection of a adenovirus harboring the PXDN gene.

Additional file 1: Figure S1. The figure file shows the proceeding of soft tissue reconstruction in more detail.

Supplemental material, sj-png-1-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Additional file 1: Figure S1. The gating strategies for the flow cytometry experiments. Data analysis was performed using FlowJo (Ashland, OR). Scatter and staining with the FITC-anti-CD41 and PE-anti-CD40L antibodies were used to gate platelet population. Cells were first gated by regions within a side scatter area (SSC-A) versus forward scatter area (FSC-A) plot, and then through gating those populations in the SSC-A versus FITC-A plots. Activated platelets were defined as FITC-anti-CD41-A positive and PE-anti-CD40L-A...

Additional file 3: Figure S3. Effects of cilostazol treatment on serum inflammatory factors. The level of serum TNF-α (a) and IL-1β (b) in each group (n = 6). The data are presented as the mean ± SEM, ***P < 0.001, ****P < 0.0001, and ns indicates no significant difference.

Additional file 4: Figure S4. Effects of cilostazol treatment on complete blood counts after CLP. a, b The level of platelets (a), WBC (b), NEUT (c), MONO (d) and LYMPH (e) (n = 5). WBC, white blood cells; NEUT, neutrophil; MONO, monocyte; LYMPH, lymphocyte. The data are presented as the mean ± SEM, *P < 0.05, ***P < 0.001, and ns indicates no significant difference.

Additional file 4: Figure S4. Effects of cilostazol treatment on complete blood counts after CLP. a, b The level of platelets (a), WBC (b), NEUT (c), MONO (d) and LYMPH (e) (n = 5). WBC, white blood cells; NEUT, neutrophil; MONO, monocyte; LYMPH, lymphocyte. The data are presented as the mean ± SEM, *P < 0.05, ***P < 0.001, and ns indicates no significant difference.

Additional file 5: Figure S5. Effect of cilostazol treatment on cardiac function after CLP. a Representative M-mode images of the four different groups. b–e Quantitative analysis of LVEF (b), LVFS (c), LVEDD (d), and LVESD (e) in each group (n = 6). These data indicate no significant difference.

Osteoarthritis (OA) is a rheumatic disease common in the elderly. AGEs are the end products of glycation reactions and play an important role in the development of OA. Etomidate is a general anesthesia-inducing agent recently reported to exert significant anti-inflammatory effects. The present study aims to explore the protective effect of Etomidate against advanced glycation end-products (AGEs)-induced reduction of extracellular matrix gene expression in chondrocytes. In the present study, we found that AGEs significantly reduced...

Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected...

Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme-producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected to...

Supplementary Data