549 Works

Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response

Xuefeng Niu, Qihong Yan, Zhipeng Yao, Fan Zhang, Linbing Qu, Chunlin Wang, Chengrui Wang, Hui Lei, Chaoming Chen, Renshan Liang, Jia Luo, Qian Wang, Lingzhai Zhao, Yudi Zhang, Kun Luo, Longyu Wang, Hongkai Wu, Tingting Liu, Pingchao Li, Zhiqiang Zheng, Yee Joo Tan, Liqiang Feng, Zhenhai Zhang, Jian Han, Fuchun Zhang … & Ling Chen
The Zika virus (ZIKV) is a mosquito-borne flavivirus that causes neonatal abnormalities and other disorders. Antibodies to the ZIKV envelope (E) protein can block infection. In this study, next-generation sequencing (NGS) of immunoglobulin heavy chain (IgH) mRNA transcripts was combined with single-cell PCR cloning of E-binding monoclonal antibodies for analysing antibody response in a patient from the early stages of infection to more than one year after the clearance of the virus. The patient's IgH...

Increasing chitosanase production in Bacillus cereus by a novel mutagenesis and screen method

Chaozheng Zhang, Yi Li, Tianshuang Zhang & Hua Zhao
Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme-producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected to...

Increasing chitosanase production in Bacillus cereus by a novel mutagenesis and screeen method

Chaozheng Zhang, Yi Li, Tianshuang Zhang & Hua Zhao
Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides. One of the prerequisites of enzyme fermentation production is to select and breed enzyme producing cells with good performance. So in the process of fermentation production, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened and identified, and a novel mutagenesis system (Atmospheric and Room Temperature Plasma (ARTP)) was selected...

High anal swab viral load predisposes adverse clinical outcomes in severe COVID-19 patients

Haibo Li, Lili Ren, Lulu Zhang, Yeming Wang, Li Guo, Conghui Wang, Yan Xiao, Ying Wang, Jian Rao, Xinming Wang, Ying Liu, Chaolin Huang, Xiaoying Gu, Guohui Fan, Hui Li, Binghuai Lu, Bin Cao & Jianwei Wang
To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Distribution characteristics of microbial community structure in atmospheric particulates of the typical industrial city in Jiangsu province, China

Xingcheng Yuan, Guangchao Li, Weihua Yang & Dan Li
In this study, Xuzhou, a typical industrial city in the north of Jiangsu Province, was chosen to investigate the pollution level of atmospheric particulates. The proportion of fine particles (PM2.5) in PM10 is larger than that of coarse particles (about 58%). The physicochemical properties of PM2.5 were analyzed by SEM and EDS. DGGE was used to study the distribution characteristics of bacterial community structure on atmospheric particulates (TSP, PM2.5 and PM10) in different functional areas...

Etomidate ameliorated advanced glycation end-products (AGEs)-induced reduction of extracellular matrix genes expression in chondrocytes

Xiaohua Sun, Jizheng Zhang, Yi Li, Wanlu Ren & Lijun Wang
Osteoarthritis (OA) is a rheumatic disease common in the elderly. AGEs are the end products of glycation reactions and play an important role in the development of OA. Etomidate is a general anesthesia-inducing agent recently reported to exert significant anti-inflammatory effects. The present study aims to explore the protective effect of Etomidate against advanced glycation end-products (AGEs)-induced reduction of extracellular matrix gene expression in chondrocytes. In the present study, we found that AGEs significantly reduced...

Additional file 7 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 7: Figure S3. miRNAs with different expression patterns in crown and leaf tissues at the winter dormancy stage, spring green-up stage and jointing stage.

Silencing of let-7b-5p inhibits ovarian cancer cell proliferation and stemness characteristics by Asp-Glu-Ala-Asp-box helicase 19A

Xiujuan Huang, Hongxia Dong, Yang Liu, Fen Yu, Shunshi Yang, Zhen Chen & Jueying Li
The emergence and recurrence of ovarian cancer are associated with ovarian cancer stem cells. For cancer treatment, gene delivery of microbubbles (MB)-mediated microRNA (miRNA) is considered as a promising approach. In this study, our aim is to investigate the effects of MB-mediated let-7b-5p inhibitor on the proliferation and stemness characteristics of ovarian cancer (OVCA) cells. Let-7b-5p inhibitor mediated by MB was prepared (termed MB-let-7b-5p inhibitor), and the effects of MB-let-7b-5p inhibitor and let-7b-5p inhibitor on...

Additional file 2 of Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions

Menglei Wang, Chenhui Yang, Kangning Wei, Miao Zhao, Liqiang Shen, Jie Ji, Li Wang, Daijing Zhang, Junqiang Guo, Yun Zheng, Juanjuan Yu, Mo Zhu, Haiying Liu & Yong-Fang Li
Additional file 2: Figure S1. Common and specific known miRNAs expressed in wheat crown tissues at four different growth and development stages.

Homeobox A5 activates p53 pathway to inhibit proliferation and promote apoptosis of adrenocortical carcinoma cells by inducing Aldo-Keto reductase family 1 member B10 expression

Danyan Chen, Zhaonan Shen, Xi Cheng, Qi Wang, Junlin Zhou, Fang Ren, Yue Sun, Hongman Wang & Rongxi Huang
Aldo-Keto Reductase Family 1 Member B10 (AKR1B10) and Homeobox A5 (HOXA5) are both down-regulated in adrenocortical carcinoma (ACC), and HOXA5 is predicted to bind to the promoter of AKR1B10. We aimed to investigate whether HOXA5 could bind to AKR1B10 to regulate ACC cells proliferation and apoptosis. The expression of AKR1B10 and HOXA5 in ACC patients and the relationship of their expression between ACC prognosis were evaluated by searching database. Then, NCI-H295R cells were overexpressed to...

SET domain containing 7 promotes oxygen-glucose deprivation/reoxygenation-induced PC12 cell inflammation and oxidative stress by regulating Keap1/Nrf2/ARE and NF-κB pathways

Xianfang Pan, Jin Fan, Fang Peng, Li Xiao & Zhiyi Yang
Oxidative stress and inflammation are implicated in the pathogenesis of cerebral ischemia-reperfusion (I/R) injury. SETD7 (SET Domain Containing 7) functions as a histone lysine methyltransferase, participates in cardiac lineage commitment, and silence of SETD7 exerts anti-inflammatory or antioxidant capacities. The effect of SETD7 in in vitro cell model of cerebral I/R injury was investigated in this study. Firstly, adrenal pheochromocytoma cell (PC12) was conducted with oxygen-glucose deprivation/reoxygenation (OGD/R) to establish cell model of cerebral I/R...

Tumor-associated neutrophils activated by tumor-derived CCL20 (C-C motif chemokine ligand 20) promote T cell immunosuppression via programmed death-ligand 1 (PD-L1) in breast cancer

Louis Boafo Kwantwi, Shujing Wang, Wenjun Zhang, Weidong Peng, Zeyu Cai, Youjing Sheng, Han Xiao, Xian Wang & Qiang Wu
Breast cancer is the leading cause of cancer-related death among women despite the significant improvement in diagnosis and treatment. Tumor-associated neutrophils have been shown to suppress antitumor functions of the host. However, how breast cancer tumor microenvironment influences the phenotype and functions of neutrophils to potentiate T cell immunosuppression is unknown. Herein, neutrophils isolated from peripheral blood of healthy donors were treated with supernatants from breast cancer cell lines or recombinant human CCL20. PD-L1 expression...

The significant immune escape of pseudotyped SARS-CoV-2 variant Omicron

Li Zhang, Qianqian Li, Ziteng Liang, Tao Li, Shuo Liu, Qianqian Cui, Jianhui Nie, Qian Wu, Xiaowang Qu, Weijin Huang & Youchun Wang
The emergence of Omicron/BA.1 has brought new challenges to fight against SARS-CoV-2. A large number of mutations in the Spike protein suggest that its susceptibility to immune protection elicited by the existing COVID-19 infection and vaccines may be altered. In this study, we constructed the pseudotyped SARS-CoV-2 variant Omicron. The sensitivity of 28 serum samples from COVID-19 convalescent patients infected with SARS-CoV-2 original strain was tested against pseudotyped Omicron as well as the other variants...

Circular RNA protein tyrosine kinase 2 (circPTK2) promotes colorectal cancer proliferation, migration, invasion and chemoresistance

Zhipeng Jiang, Zehui Hou, Wei Liu, Zhuomin Yu, Zhiqiang Liang & Shuang Chen
The dysregulated circular RNAs (circRNAs) are linked to progression and chemoresistance in colorectal cancer (CRC). However, the role of circRNA protein tyrosine kinase 2 (circPTK2) in CRC progression and chemoresistance is uncertain. The circPTK2, microRNA (miR)-136-5p, m6A ‘reader’ protein YTH domain family protein 1 (YTHDF1), β-catenin and cyclin D1 abundances were examined via quantitative reverse transcription PCR or Western blotting. The progression was investigated by cell counting kit-8 (CCK-8), colony formation, transwell and xenograft analysis....

Hyperthermia inhibits growth of nasopharyngeal carcinoma through degradation of c-Myc

Xiaole Li, Shichao Duan, Yingjuan Zheng, Yongqiang Yang, Lei Wang, Xinqiang Li, Qing Zhang, Rick F. Thorne, Wencai Li & Daoke Yang
Hyperthermia is a widely used adjunct treatment for different cancers including nasopharyngeal carcinoma (NPC). The protooncogene c-Myc is up-regulated in NPC and its expression is associated with poor prognosis. We hypothesized that c-Myc constitutes an important hyperthermia treatment target, and we investigated its contribution to hyperthermia responses in NPC. The growth of the human NPC cell lines CNE1 and CNE2 was analyzed using CCK-8 and clonogenicity assays after 43 °C hyperthermia, knockdown or overexpression of...

Long non-coding RNA PMS2L2 is down-regulated in osteoarthritis and inhibits chondrocyte proliferation by up-regulating miR-34a

Fei Yang, Min Zhao, Qinghua Sang, Changhong Yan & Zhenjun Wang
Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2 expression of each sample determined by RT-qPCR. In addition, as miR-34a has a...

Fibrinogen and antithrombin III are associated with in-hospital mortality among critically ill patients with acute kidney injury

Guangyuan Zhang, Lei Zhang, Sun Si, Tiancheng Jiang, Yi Xia, Yongkun Zhu, Xiangyu Zhang, Chi Yao, Ming Chen & Shuqiu Chen
Coagulation factors participates in the inflammatory cascade, known to play a crucial role in the development of acute kidney injury (AKI). Thus, it’s likely that some factors may be associated with AKI. Among them, low levels of fibrinogen and antithrombin III (ATIII) activity have been proved to increase mortality in patients with sepsis. Moreover, they are also reported to be associated with higher incidence of AKI. However, the association between coagulation parameters, especially fibrinogen and...

Additional file 3 of The prognostic value of IPI in patients with primary breast lymphoma, a multicenter retrospective study

Kexin Feng, Shuangtao Zhao, Qingyao Shang, Guangdong Qiao, Jiaxiang Liu, Chenxuan Yang, Ya Wei, Yalun Li, Fei Ren, Lixue Xuan, Xiang Wang & Xin Wang
Additional file 3: Figure S1. ROC curves for 5-year survival of IPI and predictive factors significant in univariate analysis. A, ROC curves for 5-year OS of IPI and predictive factors significant in univariate analysis. B, ROC curves for 5-year PFS of IPI and predictive factors significant in univariate analysis.

Additional file 1 of Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis

Bo Cheng, Mengyu Du, Shuxuan He, Lan Yang, Xi Wang, Hui Gao, Haiqing Chang, Wei Gao, Yan Li, Qiang Wang & Yansong Li
Additional file 1: Fig. S1. The gating strategies for the flow cytometry experiments. Data analysis was performed using FlowJo (Ashland, OR). Scatter and staining with the FITC-anti-CD41 and PE-anti-CD40L antibodies were used to gate platelet population. Cells were first gated by regions within a side scatter area (SSC-A) versus forward scatter area (FSC-A) plot, and then through gating those populations in the SSC-A versus FITC-A plots. Activated platelets were defined as FITC-anti-CD41-A positive and PE-anti-CD40L-A...

Additional file 2 of Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis

Bo Cheng, Mengyu Du, Shuxuan He, Lan Yang, Xi Wang, Hui Gao, Haiqing Chang, Wei Gao, Yan Li, Qiang Wang & Yansong Li
Additional file 2: Fig. S2. KEGG enrichment of differential expressed genes in septic patients compared with healthy person. a The volcano plot was constructed using the fold change values and P-adjust. Red dots indicate upregulated genes; blue dots indicate downregulated genes. b The heatmap of the differential gene expression, where different colors represent trends of gene expression in different tissues. c, d The enriched KEGG signaling pathways were selected to demonstrate the primary biological actions...

Additional file 2 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 2 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 2: Supplementary Figure S2. Correlation analysis of the 76 NRGs for CESC (A), OV (B), UCEC (C), and UCS D. Red indicates a positive correlation; blue indicates a negative correlation.

Additional file 6 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 6: Supplementary Figure S6. Expression values of NRGs in prognostic signature of OV. A-D Expression values of NRGs in TCGA for BACH2 (A), MLKL (B), MYC (C), MYCN (D), and SIRT2 (E). F-J Expression values of NRGs in our cohort for BACH2 (F), MLKL (I), MYC (G), MYCN (H), and SIRT2 (J).

Additional file 7 of A comprehensive pan-cancer analysis of necroptosis molecules in four gynecologic cancers

Jianfeng Zheng, Xintong Cai, Yu Zhang, Huihui Wang, Li Liu, Fengling Tang, Linying Liu & Yang Sun
Additional file 7: Supplementary Figure S7. Clinical value of risk score by independent prognostic analysis. A-H The Univariate Cox regression analysis and Multivariate Cox regression analysis for CESC (A-B), OV (C-D), UCEC (E-F), and UCS G-H.

Additional file 5 of tRF-3013b inhibits gallbladder cancer proliferation by targeting TPRG1L

Lu Zou, Yang Yang, Biyu Zhou, Weijian Li, Ke Liu, Guoqiang Li, Huijie Miao, Xiaoling Song, Jiahua Yang, Yajun Geng, Maolan Li, Runfa Bao & Yingbin Liu
Additional file 5: Fig S3. Verification of tRF-3013b overexpression and inhibition. (A, B) Relative tRF-3013b expression with NC or tRF-3013b mimic transfection in NOZ and GBC-SD cells. (C, D) Relative tRF-3013b expression with Anti-NC or Anti-tRF-3013b transfection in NOZ and GBC-SD cells. **P < 0.01, ***P < 0.001.

Registration Year

  • 2023
    28
  • 2022
    486
  • 2021
    25
  • 2020
    10

Resource Types

  • Image
    549

Affiliations

  • Central South University
    549
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    414
  • Shanghai Jiao Tong University
    386
  • Zhejiang University
    341
  • Sichuan University
    337
  • Fudan University
    333
  • Sun Yat-sen University
    323
  • Huazhong University of Science and Technology
    322
  • Shandong University
    288
  • Capital Medical University
    284