Additional file 1: Figure S1. Enrichment of differentially expressed genes in lung tissues of neonatal mice with intrauterine inflammation. A Immunofluorescence detection of Pro-SFTPC in the lung tissues of neonatal mice (green, DAPI: blue). C, D GO and KEGG enrichment of the upregulated genes. E, F GO and KEGG enrichment of the downregulated genes.Q-value ≤ 0.05 and fold-change ≥ 2 were used as the thresholds for screening the differentially expressed genes. Data show mean ±...

Additional file 1: Figure S1. Enrichment of differentially expressed genes in lung tissues of neonatal mice with intrauterine inflammation. A Immunofluorescence detection of Pro-SFTPC in the lung tissues of neonatal mice (green, DAPI: blue). C, D GO and KEGG enrichment of the upregulated genes. E, F GO and KEGG enrichment of the downregulated genes.Q-value ≤ 0.05 and fold-change ≥ 2 were used as the thresholds for screening the differentially expressed genes. Data show mean ±...

Additional file 5: Figure S5. The expression of DNA methylation related enzymes in lung tissues and TNF-α-treated MLE-12 cells. A Dnmt1, Dnmt3a, Mbd1, and Tet1 expression analysed using RT-qPCR in lung tissues of neonatal mice. B TNF-α expression analysed using RT-qPCR in lung tissues of neonatal mice. C Dnmt1, Dnmt3a, Mbd1, and Tet1 expression analysed using RT-qPCR in TNF-α-treated MLE-12 cells. D Serpine1 expression analysed using RT-qPCR in TNF-α-treated MLE-12 cells. Data show mean ±...

Additional file 2: Supplementary figure 2. The relationship between immune-related function and prognosis of CLL patients.

Additional file 4: Fig. S3. A Schematic diagram of the binding sites of miR-532-3p on CEBPA-DT. B Relative luciferase activities of miR-532-3p binding sites on CEBPA-DT were measured by luciferase reporter assays in indicated 293T cells. C RIP-qPCR assays showed the enrichment of CEBPA-DT on AGO2 relative to IgG in indicated HCCLM9 cells. D Subcellular localization of hnRNPC in indicated HCCLM9 and SNU-449 cells were measured by subcellular protein fractionations and western blot assays. E-F...

Additional file 4: Fig. S3. A Schematic diagram of the binding sites of miR-532-3p on CEBPA-DT. B Relative luciferase activities of miR-532-3p binding sites on CEBPA-DT were measured by luciferase reporter assays in indicated 293T cells. C RIP-qPCR assays showed the enrichment of CEBPA-DT on AGO2 relative to IgG in indicated HCCLM9 cells. D Subcellular localization of hnRNPC in indicated HCCLM9 and SNU-449 cells were measured by subcellular protein fractionations and western blot assays. E-F...

Additional file 1: Figure S1. Pie chart shows the number of differentially expressed lncRNAs in each category.

Additional file 4: Figure S3. Volcano plot showing the log2 fold change of 517 significantly differentially expressed miRNAs in CRC patients from the TCGA database.

Supplementary Material 4

Supplementary Material 4

Additional file 13: Figure S9. The transcriptional expression of VAV1, RHOA, and ZC3HAV1 was positively correlated to the distribution of EMT scores in the HCC cohort from TCGA (A, D, and G) and ICGC (B, E, and H) database, and GSE14520 (C, F, and I) dataset. EMT: epithelial-mesenchymal transition. r: Pearson's correlation coefficient.

Additional file 9: Figure S6. The top 20 co-expressed gene signatures of each survival-related markers of the STING pathway (A-E) and their functional enrichment analysis including KEGG pathways (F) and immune processes (G). R: Pearson’s correlation coefficient. KEGG: Kyoto Encyclopedia of Genes and Genomes.

Additional file 9: Figure S6. The top 20 co-expressed gene signatures of each survival-related markers of the STING pathway (A-E) and their functional enrichment analysis including KEGG pathways (F) and immune processes (G). R: Pearson’s correlation coefficient. KEGG: Kyoto Encyclopedia of Genes and Genomes.

Additional file 4. Tucidinostat plus checkpoint blockade induces significant antitumor immunity. a Schema of the experiment. Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). The proportion of intratumoral CD8+ T cells...

Additional file 4. Tucidinostat plus checkpoint blockade induces significant antitumor immunity. a Schema of the experiment. Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). The proportion of intratumoral CD8+ T cells...

Additional file 5. Tucidinostat plus checkpoint blockade induces CCL5 secretion. a Mouse CT26 cells (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with different doses (12.5, 25, 75 mg/kg, gavage, daily, n=5) of tucidinostat. The expression of CCL5 in peripheral blood serum from CT26 tumor-bearing mice on day 10 post treatment initiation. Mouse CT26 cells (5 ×...

Additional file 3: Fig. S3. Show the correlation of L_cancer RiskScore and L_cancer hub genes expression with immune infiltration level in L_cancer patients.

Additional file 3: Figures S2. GO enrichment analysis of the 12 differentially expressed proteins.

LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 3: Figure S3. The effect of Serpine1 overexpression in lung epithelial cells. A Serpine1 expression analysed using RT-qPCR in Serpine1-overexpressing MLE-12 cells. B–D Serpine1 expression analysed using RT-qPCR, western blot and Immunofluorescence in Serpine1-overexpressing A549 cells. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing A549 cells. G p16 expressions analysed using western blot in Serpine1-overexpressing BEAS-2B cells. Data show mean ± SEM; Data was analysed using unpaired t-tests.

Additional file 4: Figure S4. Enrichment of differentially expressed genes in Serpine1-overexpressing MLE-12 cells. A, B GO and KEGG enrichment of the upregulated genes. C, D GO and KEGG enrichment of the downregulated genes. Q-value ≤ 0.05 and fold-change ≥ 2 were used as the thresholds for screening the differentially expressed genes.

Additional file 2: Figure S2. Genes expression levels in lung tissues of neonatal mice (A) The mRNA expression of differentially expressed genes analysed using RT-qPCR in lung tissues of neonatal mice. B, C Nr4a1 expression analysed using western blot in lung tissues of neonatal mice. D Serpine1 expression in lung tissues of neonatal mice detected using RT-qPCR on P1, P7, and P21. E, F Serpine1 expression analysed using RT-qPCR and western blot in siRNA-treated neonatal...

Additional file 3: Figure S3. The effect of Serpine1 overexpression in lung epithelial cells. A Serpine1 expression analysed using RT-qPCR in Serpine1-overexpressing MLE-12 cells. B–D Serpine1 expression analysed using RT-qPCR, western blot and Immunofluorescence in Serpine1-overexpressing A549 cells. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing A549 cells. G p16 expressions analysed using western blot in Serpine1-overexpressing BEAS-2B cells. Data show mean ± SEM; Data was analysed using unpaired t-tests.

Additional file 5: Figure S5. The expression of DNA methylation related enzymes in lung tissues and TNF-α-treated MLE-12 cells. A Dnmt1, Dnmt3a, Mbd1, and Tet1 expression analysed using RT-qPCR in lung tissues of neonatal mice. B TNF-α expression analysed using RT-qPCR in lung tissues of neonatal mice. C Dnmt1, Dnmt3a, Mbd1, and Tet1 expression analysed using RT-qPCR in TNF-α-treated MLE-12 cells. D Serpine1 expression analysed using RT-qPCR in TNF-α-treated MLE-12 cells. Data show mean ±...

Additional file 7: Figure S7. Schematic representation of the molecular mechanisms underlying IUI-induced lung injury in neonatal mice.