Transarterial chemoembolization is a standard treatment for intermediate-stage hepatocellular carcinoma (HCC). This study evaluated the anti-tumor effect of the semi-interpenetrating network (IPN) hydrogel as a novel embolic material for trans-portal vein chemoembolization (TPVE) in vivo. A nude mice orthotopic HCC model was established, followed by TPVE using IPN hydrogel loaded with or without cisplatin. Portal vein blockade was visualized by MRI and the development of tumor was monitored by IVIS Spectrum Imaging. Tumor proliferation and...

Transarterial chemoembolization is a standard treatment for intermediate-stage hepatocellular carcinoma (HCC). This study evaluated the anti-tumor effect of the semi-interpenetrating network (IPN) hydrogel as a novel embolic material for trans-portal vein chemoembolization (TPVE) in vivo. A nude mice orthotopic HCC model was established, followed by TPVE using IPN hydrogel loaded with or without cisplatin. Portal vein blockade was visualized by MRI and the development of tumor was monitored by IVIS Spectrum Imaging. Tumor proliferation and...

Supplmentary figure 1

Supplementary figures 1 and 2

Supplemental material, sj-tif-1-tct-10.1177_15330338231154092 for Genetic and Clinical Characteristics of Patients with Philadelphia-Negative Myeloproliferative Neoplasm Carrying Concurrent Mutations in JAK2V617F, CALR, and MPL by Yan Wang, Fei Ran, Jin Lin, Jing Zhang and Dan Ma in Technology in Cancer Research & Treatment

Supplemental material, sj-tif-2-tag-10.1177_17562848231155023 for An explainable artificial intelligence system for diagnosing Helicobacter Pylori infection under endoscopy: a case–control study by Mengjiao Zhang, Jie Pan, Jiejun Lin, Ming Xu, Lihui Zhang, Renduo Shang, Liwen Yao, Yanxia Li, Wei Zhou, Yunchao Deng, Zehua Dong, Yijie Zhu, Xiao Tao, Lianlian Wu and Honggang Yu in Therapeutic Advances in Gastroenterology

Additional file 1. Cholesterol micelle suspension.

Additional file 6. Nerve endings terminate adjacent to ChAT-positive or α7nAChR-positive cell in spleen. Synaptophysin-positive (green) nerve endings were found in close proximity to ChAT-positive (A: red) or α7nAChR-positive (B: red) in LIPUS group of mice (especially white arrow). In statistical analysis, the number of target cell in LIPUS group is normalized to EAM group (bottom). (n=4) * represents LIPUS-0.35 MPa vs. EAM. (Data were shown as the mean ± SD, unpaired T test, Mann-Whitney...

Additional file 2. LIPUS stimulated localization on the spleen and measurement of mouse spleen dimensions. Mice were anesthetized with isoflurane before LIPUS stimulation. Since the spleen is shallow, it can be clearly outlined and marked through the thin skin (A-B). To confirm the spleen's location, the mouse skin was turned over (C). The spleen is consistently located halfway between the shoulder and hip joints of the animal, so the landmarks can be used to target...

Additional file 5. Effect of LIPUS on the immunohistochemical staining of CX3CR1 in myocardium. Representative immunohistochemical staining of CX3CR1 in myocardium of control, EAM, and LIPUS group (n=4). Scale bar represents 50 μm. EAM: Experimental Autoimmune Myocarditis; LIPUS: low-intensity pulsed ultrasound.

Additional file 7. The effect of splenic denervation on vessels of EAM mice. A. HE staining for spleen histological signs of EAM mice and EAM+SDN mice. B. Representative images of whole spleen sections of EAM mice and EAM+SDN mice. Blue, nuclear staining. Green, CD31 is a sensitive and specific marker for vascular endothelial injury (especially white arrow). EAM: Experimental Autoimmune Myocarditis; SDN: Splenic Denervation; HE: hematoxylin and eosin.

Additional file 1: Fig. S1. Experimental flow chart.

Supplemental material, sj-tiff-2-jhc-10.1369_00221554231161693 for Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel–referenced Evaluation by Jun Jiang, Raymond Moore, Clarissa E. Jordan, Ruifeng Guo, Rachel L. Maus, Hongfang Liu, Ellen Goode, Svetomir N. Markovic and Chen Wang in Journal of Histochemistry & Cytochemistry

Supplemental material, sj-jpg-3-jcb-10.1177_0271678X231167729 for An optical clearing imaging window: Realization of mouse brain imaging and manipulation through scalp and skull by Wei Feng, Chun-jie Liu, Lisi Wang and Chao Zhang in Journal of Cerebral Blood Flow & Metabolism

Additional file 4: Figure S4. No significant difference was observed in AS lesions, cholesterol, triglycerides between ABCB5+ MSC treated and untreated mice. 7 days post DLFA, blood and aorta samples from mice were taken. A representative HE stained sections of the aorta from the A control group and B ABCB5+ MSC treated mice. A1 and B1 displays atherosclerotic vessel wall while A2 and B2 displays normal vessel wall (scale bar is 50 μm in Figure...

Additional file 2: Figure S2. The Cobb angle, sagittal parameters and LL before (A,C and E) and after (B,D and F) operation of case 23.

Additional file 2: Fig. S2. Binding of KPNA2 to STAT3 was increased under hypoxia for 24 h relative to normoxia. (A)Western blotting analysis was used to detect KPNA2 of IP STAT3 hypoxia for 24 h vs under normoxia. Relative quantitative statistical analysis was performed on protein expression levels. Each experiment was repeated three times. Each experiment was repeated three times. * p<0.05.

Additional file 1: full-length blot gels are presented in Supplementary Figure 2D.

Additional file 2: full-length blot gels are presented in Supplementary Figure 2E.

Additional file 1: Fig. S1. Detection of RNA modifications in mouse multiple tissues RNA. (A) Experimental procedures for detecting and quantifying RNA modifications in mouse tissues RNA. (B) List of applied nucleobase standards. (C) The percentage of modified and unmodified four nucleotides (Adenine, Uracil, Cytosine and Guanine) in mouse mRNA and the relative proportion of detected RNA modifications across multiple tissues (The sum of all RNA modification was considered as 100 and the percentage was...

Additional file 6: Fig. S6. Scatter plot comparison of transcriptome between GC-2spd which transfected with NC group testis tRNA enriched fragments and hypoxia group testis tRNA enriched fragments.

Additional file 9: Fig. S9. Original uncropped images for denatured urea gel, northern blot and western blot. (A) Denatured urea gel image of liver total RNA for Fig. 6D. (B-D) Northern blot images of tRNAAla (B), tRNAVal (C), tRNAGly (D) for Fig. 6D. (E) Denatured urea gel image of GC-2spd cell total RNA for Fig. 7D. (F-H) Northern blot images of tRNAAla for Fig. 7D. (I-K) Western blot images of Actin (I), eIF4A (J) and...

Additional file 8: Fig. S8. Original uncropped images for tRNA enriched fragments stability analysis. (A-D) The original uncropped images of native gel to analyze liver tRNA-enriched fragments (A), testis tRNA enriched fragments stability (B), liver tRNAAla and liver tRNAGly (C), and liver tRNAVal (D) stability against RNase A/T1 and fetal bovine serum.

Additional file 1: Figure S1. The difference of the density of CD8+TILs, PD-1+cells, PD-L1+cells, TIM-3+cells, PD-1+CD8+TILs, PD-L1+CD8+TILs and TIM-3+CD8+TILs, and the difference of the percentage of PD-1+CD8+TILs, PD-L1+CD8+TILs and TIM-3+CD8+TILs were analyzed based on the T stage, N stage and Lauren's classification.

Additional file 1: Supplementary figure 1. Landscape of the expression levels and genetic alterations of 5mC/m6A regulators across 33 cancer types. (A) Gene expression profiling of 5mC/m6A regulators across pan-cancer. For a given 5mC/m6A regulator in each cancer type, the median expression value is shown. (B, C) Spearman correlations of CNVs with transcript levels of 5mC/m6A regulators in pan-cancer, reflecting the gene expression significantly affected by CNVs. (D, E) Pie chart for heterozygous and homozygous...