Additional file 11. Figure S11. Heatmap showing cluster-specific gene profiles. (a) Macrophage-specific gene profiles. (b) Neutrophil-specific gene profiles. (c) T cell-specific gene profiles. (d) NK cell-specific gene profiles. (e) B cell-specific gene profiles. (f) Monocyte-specific gene profiles. (g) cDC-specific gene profiles. (h) pDC-specific gene profiles. (i) Mast-specific gene profiles.

Isolation and identification of GSCs. A. a, c: Cells grew into spheres in serum-free medium. b, d: Spheres generated again from a single cell. B. Individual undifferentiated GSCs-87 and GSCs-251 stained for Nestin (green) and CD133 (red) by immunofluorescence analysis. C. Cell spheres were differentiated and then stained for GFAP (green) and β-tubulin III (red) by immunofluorescence analysis. Nuclei (blue) were labeled with DAPI. Images are representative of independent experiments (n = 5). Scale bars...

Transarterial chemoembolization is a standard treatment for intermediate-stage hepatocellular carcinoma (HCC). This study evaluated the anti-tumor effect of the semi-interpenetrating network (IPN) hydrogel as a novel embolic material for trans-portal vein chemoembolization (TPVE) in vivo. A nude mice orthotopic HCC model was established, followed by TPVE using IPN hydrogel loaded with or without cisplatin. Portal vein blockade was visualized by MRI and the development of tumor was monitored by IVIS Spectrum Imaging. Tumor proliferation and...

Transarterial chemoembolization is a standard treatment for intermediate-stage hepatocellular carcinoma (HCC). This study evaluated the anti-tumor effect of the semi-interpenetrating network (IPN) hydrogel as a novel embolic material for trans-portal vein chemoembolization (TPVE) in vivo. A nude mice orthotopic HCC model was established, followed by TPVE using IPN hydrogel loaded with or without cisplatin. Portal vein blockade was visualized by MRI and the development of tumor was monitored by IVIS Spectrum Imaging. Tumor proliferation and...

Additional file 5: Figure S5. Forest plot for adverse events at 4 weeks by concomitant medications.

Additional file 6: Figure S6. Forest plot for withdrawals due to adverse events in ADA and control groups

Supplmentary figure 1

Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV- 1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplificationlateral flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative realtime PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP reaction is optimal at 65◦C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to...

Additional file 3: Fig. S2. A The expression levels of CEBPA-DT in different hepatoma cell lines measured by RT-qPCR. B Subcellular location of CEBPA-DT in indicated Huh7 cells examined by subcellular RNA fractionations and RT-qPCR analysis, β-actin and U3 were used as cytoplasmic and nuclear endogenous control, respectively. C The expression levels of CEBPA-DT in HCCLM9 and SNU-449 cells transfected with control siRNA or siRNA against CEBPA-DT. D qPCR quantification of the expression levels of...

Additional file 5: Fig. S4. A Clustering heatmap of significant differentially expressed mRNAs in HCCLM9 cells transfected with negative control or CEBPA-DT siRNAs. B Significantly enriched pathways annotated by the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. C Significantly enriched pathways annotated by the Gene Ontology (GO) database. D Significantly enriched pathways annotated by the Molecular Signatures Database (MSigDB). E The binding sites of hnRNPC and 3’UTR of DDR2 were predicted by PRIdictor database...

Additional file 7: Fig. S6. A-B Scratch wound-healing assays showed cell migration capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. C-D Transwell migration and matrigel invasion assays showed cell migration and invasion capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. E Representative images (fluorescence) of lung metastatic...

Supplemental material, sj-tif-1-tct-10.1177_15330338231154092 for Genetic and Clinical Characteristics of Patients with Philadelphia-Negative Myeloproliferative Neoplasm Carrying Concurrent Mutations in JAK2V617F, CALR, and MPL by Yan Wang, Fei Ran, Jin Lin, Jing Zhang and Dan Ma in Technology in Cancer Research & Treatment

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 2: Figures S1. Confirmation of exosomes by transmission electron microscopy (TEM).

Additional file 3: Fig. S3. Show the correlation of L_cancer RiskScore and L_cancer hub genes expression with immune infiltration level in L_cancer patients.

Additional file 3. Tucidinostat plus checkpoint blockade induces improved therapeutic efficacy. a Schema of the experiment. For bioluminescence imaging (BLI) in vivo, mouse 4T1-luc (5 × 105 cells) were engrafted into the flank of BALB/c mice. When established tumors were palpable 7 days after tumor cells inoculation, mice were treated with vehicle (DMSO, n=7), tucidinostat (25 mg/kg, gavage, daily, n=7), aPD-L1 (200 μg, i.p. injection, once every 3 days, n=7), or combination (n=7). Tumors volume...

Supplemental material, sj-png-1-taj-10.1177_20406223221143245 for Chest X-ray features facilitate screening for pulmonary hypertension caused by fibrosing mediastinitis by Mingfang Zhou, Bo Li, Yaling Chen, Aqian Wang, Yining Zhu, Yu Li, Hongling Su, Jingchun Fan, Yan Zhang and Yunshan Cao in Therapeutic Advances in Chronic Disease

Additional file 2. Supplement Figure.

Additional file 3: Fig S1. tRFs expression in GBC tissues. (A) Bar chart of the sequence read length distribution of tRF and tiRNA. (B) Pie chart of the distribution of subtype tRF and tiRNA.

Additional file 5: Fig S3. Verification of tRF-3013b overexpression and inhibition. (A, B) Relative tRF-3013b expression with NC or tRF-3013b mimic transfection in NOZ and GBC-SD cells. (C, D) Relative tRF-3013b expression with Anti-NC or Anti-tRF-3013b transfection in NOZ and GBC-SD cells. **P < 0.01, ***P < 0.001.

Additional file 6: Fig S4. tRF-3013b was related to the cell cycle progression. (A) Heatmap showing the differential genes between NOZ cells transfected with NC or tRF-3013b mimic. (B) Volcano plot showing the differential genes between NOZ cells transfected with NC or tRF-3013b mimic. (C) GO enrichment distribution map showing the related pathways after transfection with NC or tRF-3013b mimic in NOZ cells. (D) Significance of related pathways by GO enrichment analysis (BP, biological process;...

Additional file 7: Fig S5. TPRG1L promoted GBC cell proliferation. (A) CCK8 assay and (B) colony formation were performed to evaluated proliferation capacity after transfection with vector or TPRG1L. (C) Relative protein expression of TPRG1L, p65, p50, c-myc, CDK2, and p-CDK2 after transfection with vector or TPRG1L. (D) Representative IHC micrographs showing TPRG1L protein expression in GBC tissues with high or low miR-143-3p expression. *P < 0.05, **P < 0.01, ***P < 0.001.

Supplementary figures 1 and 2

Additional file 3: Fig. S3. qRT-PCR analysis of FZD2, FZD3, FZD4 and FZD7 in NSCLC cells and HBE cells. All data were normalized to the expression level in HBE cells. *, P < 0.05, **, P < 0.01***, P < 0.001.

Additional file 12: Figure S4. Representative immunohistochemical staining images for GSTT1 in bone marrows of Control, Model, S+Rg, S, and Rg groups.