Additional file 2: Fig. S1. A Schematic diagram of the genomic locus of CEBPA-DT (www.ncbi.nlm.nih.gov/). B The protein-coding potential of CEBPA-DT predicted by Open reading frame (ORF) Finder software prediction ( https://www.ncbi.nlm.nih.gov/orffinder/ ). C The protein-coding potential of CEBPA-DT predicted by Coding-Potential Assessment Tool ( wlcb.oit.uci.edu/cpat/ ).

Additional file 6: Fig. S5. A-B The expression levels of DDR2 were measured by western blot and RT-qPCR in indicated HCCLM9 and SNU-449 cells transfected with negative control siRNA or siRNAs targeting DDR2. C-D Cell migration capacity of indicated HCCLM9 and SNU-449 cells were measured by scratch wound-healing assays. Scale bar, 100μm. E-F Cell migration and invasion capacity of indicated HCCLM9 and SNU-449 cells were measured by transwell migration and matrigel invasion assays. Scale bar,...

Additional file 7: Fig. S6. A-B Scratch wound-healing assays showed cell migration capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. C-D Transwell migration and matrigel invasion assays showed cell migration and invasion capacity of indicated Hep3B and Huh7 cells stably transfected with lentivirus of shDDR2 vectors and control/ CEBPA-DT overexpressing vectors. Scale bar, 100μm. E Representative images (fluorescence) of lung metastatic...

Supplementary Material 1

Additional file 1: Fig. S1 The distributions of variant allele frequencies (VAFs) of ARID1A, ARID1B, ARID2, PBRM1, SMARCA4, and SMARCB1. The median VAFs of the above genes were 16.1%, 13.4%, 13.3%, 17.2%, 15.2%, and 16.7%, respectively. Red solid line, median; black dotted line, quartiles.

Additional file 3: Fig. S3 The signaling pathway enrichment of the variated genes in the SWI/SNF-mutant tumors by GO analysis. The GO analysis was performed on all the mutated genes in 1001 SWI/SNF-mutant samples.

Supplementary materials to the article ' National survey on unplanned readmissions for diabetic kidney disease: Insight from a new paradigm of obesity'

The pollution of heavy metals in the soil around the tailings pond was evaluated. The content of heavy metals in the aboveground and underground parts of plants around the tailings pond was determined. The remediation potential of plants for heavy metals was evaluated.

The pollution of heavy metals in the soil around the tailings pond was evaluated. The content of heavy metals in the aboveground and underground parts of plants around the tailings pond was determined. The remediation potential of plants for heavy metals was evaluated.

Additional file 3: Supplemental Figure 3. Negative and lowgrades of B7-H3 expression in the pathological sections of 60 cases ofosteosarcoma.

Additional file 5: Supplementary Figure S5. The ROC curves for the risk model in the four GCs. A-C The ROC curves of CESC for training (A), validation (B), and total (C) sets. D-F The ROC curves of OV for training (D), valida©n (E), and total (F) sets. G-I The ROC curves of UCEC for training (G), validation (H), and total (I) sets. J-L The ROC curves of UCS for training (J), validation (K), and total...

Additional file 7: Supplementary Figure S7. Clinical value of risk score by independent prognostic analysis. A-H The Univariate Cox regression analysis and Multivariate Cox regression analysis for CESC (A-B), OV (C-D), UCEC (E-F), and UCS G-H.

Additional file 8: Supplementary Figure S8. The Nomogram model based on risk model and clinical features for GCs. A-B The Nomogram (A) and calibration curve (B) for CESC. C-D The Nomogram (C) and calibration curve (D) for OV. (E-F) The Nomo©m (E) and calibration curve (F) for UCEC. G-H The Nomogram (G) and calibration curve (H) for UCS.

Additional file 3: Supplementary Figure 3.

Additional file 4: Supplementary Figure 4.

Additional file 5: Supplementary Figure 5.

Additional file 1: Supplementary Figure S1. Mutation frequency and expression variation of the 76 necroptosis-related genes (NRGs). A-D Mutation frequency of NRGs in patients with CESC (A), OV (B), UCEC (C), and UCS D. The small figure above shows the TMB, the number on the right shows the mutation frequency of each NRG, and the figure on the right shows the proportion of each vari©. E Expression levels of NRGs in four gynecological tumors. The...

Additional file 2: Figure S2. Raw western blots of GFAP (a), C3d (b), S100a10(c) and β-actin (d) in Figure 4b 12% Gel, 0.45 µm PVDF membrane.

Additional file 5: Figure S5. Raw western blots of GFAP(a), C3d (b), S100a10(c) and β-actin (d) in Figure 7c 12%Gel, 0.45 µm PVDF membrane.

Additional file 1: SupplementaryFigure 1. The top 10 cited countrys related to this field.

Additional file 2: SupplementaryFigure 2. The network map of top 10 institutions (A) and the top 10 cited networkmap of institutions in this field (B).

Additional file 2: Fig. S1. Multicollinearity analysis for Hb, ALT and ALB.

Osteosarcoma (OS) is an aggressive malignant tumor with a high rate of lung metastasis and a lack of therapeutic targets. Although the anomalous expression of long non-coding RNA (lncRNA) has been extensively documented in human cancer, its contribution to OS metastasis remains poorly understood. In this study, we found that MIR205 host gene (MIR205HG) was significantly elevated in human OS tissues, especially in metastatic OS tissues. Stable knockdown of MIR205HG inhibited OS cell invasion and...

The alkaloids of Narcissus tazetta L. var. Chinensis Roem (Amaryllidaceae) have antitumor and antiviral activities. However, the immunopharmacological effects of one of its constituents, pseudolycorine chloride (PLY), have not been reported yet. We evaluated the effect of PLY on myeloid-derived suppressor cells (MDSCs) expansion and differentiation into monocyte-like MDSCs (M-MDSCs) and examined whether PLY alleviates Th17 cell-mediated experimental autoimmune encephalomyelitis (EAE), a murine model of multiple sclerosis (MS). In vitro, MDSCs were treated with PLY...

Symbiochlorum hainandiae S.Q. Gong & Z.Y. Li, 2018 is a unicellular green alga belonging to Ulvophyceae, Chlorophyta, and plays important roles in coral reef ecosystem. In this study, high-throughput sequencing technology is used to sequence and assemble the chloroplast genome of S. hainandiae. The complete chloroplast genome of S. hainandiae was 158, 96 bp with the GC content of 32.86%. A total of 126 genes were identified, including 98 protein-coding genes, 26 tRNA, and 2...