831 Works

Role of lncRNA LINC01194 in hepatocellular carcinoma via the miR-655-3p/SMAD family member 5 axis

Yang Liu, Jie Liu, Junkai Cui, Ruolei Zhong & Guoyang Sun
Long non-coding RNAs (lncRNAs) are involved in developing hepatocellular carcinoma (HCC). The present study explored the role of lncRNA LINC01194, which is upregulated in HCC tissues and might be a vital regulator in HCC progression. Levels of LINC01194, microRNA (miR)-655-3p, and SMAD family member 5 (SMAD5) were assessed using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The bioactivity of Huh-7 cells was assessed using cell counting kit-8 and transwell assays and flow cytometry. Western...

Long non-coding RNA ZFAS1 regulates cell proliferation and invasion in cervical cancer via the miR-190a-3p/KLF6 axis

Yuehui Su, Wenjing Hou, Chunyan Zhang, Pengcheng Ji, Rui Hu, Qiongying Zhang, Yao Wang, Panpan Li, Huiping Zhang, Yueyue Chen, Xiaodong Zhang & Mengzhen Zhang
Long non-coding RNA (lncRNA) ZFAS1 (zinc finger antisense 1) was demonstrated to play critical roles in various cancer progression. However, the functions of ZFAS in cervical cancers (CC) are unclear. Human CC cell lines were used for in vitro experiments. RT-qPCR (Real Time Quantitative PCR) was performed to detect the expression of ZFAS1, microRNA-190a-3p (miR-190a-3p) and Kruppel-like factor 6 (KLF6). Cell proliferation, invasion and migration assays were used to investigate biological behaviors of CC cells...

Long noncoding RNA H19 suppresses cardiac hypertrophy through the MicroRNA-145-3p/SMAD4 axis

Hao Wang, Xiaoqing Lian, Wei Gao, Jie Gu, Haojie Shi, Yao Ma, Yafei Li, Yi Fan, Qiming Wang & Liansheng Wang
Sustained cardiac hypertrophy (CH) contributes to many heart diseases. Long noncoding RNAs (lncRNAs) collectively play critical roles in cardiovascular diseases (CVDs). However, the roles of lncRNA H19 in CH are still unclear. A CH model was constructed utilizing isoproterenol (ISO). We demonstrated H19 could participate in regulating ISO-induced CH development both in vivo and in vitro. The online databases DIANA and TargetScan were used to predict the targets of H19 and MicroRNA-145-3p (miR-145-3p), respectively. Luciferase...

A novel subtype to predict prognosis and treatment response with DNA driver methylation–transcription in ovarian cancer.

Yan Hou, Kang Li, Liuying Wang, Shuang Li, Min Zhang,, Yue Huang, Meng Wang, Liuchao Zhang & Zhenyi Xu
TableS1. Clinical data of ovarian cancer patients from TCGA and ICGC.
TableS2.Identified DNA driver methylation (DNAme) in TCGA, ICGC and GSE146556.
TableS3. The correlation between DNAme and corresponding mRNA expression in TCGA, ICGC and GSE146556. TableS4.Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of DNAme-associated mRNA in TCGA.
TableS5. Univariate Cox regression analysis of DNAme-associated mRNA. (P < 0.05)
TableS6. Analysis of pathway enrichment (KEGG) and Gene Ontology (GO) of...

Additional file 11 of Exploring AI-2-mediated interspecies communications within rumen microbial communities

Xiaozhen Liu, Qinmeng Liu, Sihuai Sun, Hengxi Sun, Yao Wang, Xihui Shen & Lei Zhang
Additional file 11: Figure S5. Expression of the predicted AI-2 synthases and receptors within a rumen microbial metatranscriptome at the phylum level. (A) The number of bacterial genomes containing AI-2-related genes and the percentage of corresponding genomes that expressed these genes. (B) The number of bacterial genomes containing luxS synthases and the percentage of the corresponding genomes that expressed luxS genes at the phylum level. (C) The number of bacterial genomes containing lsrB receptors and...

Additional file 10 of RNA interference-mediated silencing of DNA methyltransferase 1 attenuates neuropathic pain by accelerating microglia M2 polarization

Ying Tan, Zongjiang Wang, Tao Liu, Peng Gao, Shitao Xu & Lei Tan
Additional file 10.

Additional file 4 of RNA interference-mediated silencing of DNA methyltransferase 1 attenuates neuropathic pain by accelerating microglia M2 polarization

Ying Tan, Zongjiang Wang, Tao Liu, Peng Gao, Shitao Xu & Lei Tan
Additional file 4.

Additional file 7 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 7 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 7: Figure S4. Yields of saturated mutants of three amino acid residues of Cv-ωTA converting cinnamaldehyde to cinnamylamine. (A) The production of cinnamylamine in saturated mutants of at the Phe22 site after adding cinnamaldehyde. (B) The production of cinnamylamine in saturated mutants of at the Tyr168 site after adding cinnamaldehyde. (C) The production of cinnamylamine in saturated mutants of at the Ala231 site after adding cinnamaldehyde. The mutant with the highest cinnamylamine production...

Additional file 6 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Additional file 2 of Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Yanchun Li, Bing Xu, Xueying Ren, Luyang Wang, Yaqing Xu, Yefeng Zhao, Chen Yang, Chen Yuan, Huanjuan Li, Xiangmin Tong, Ying Wang & Jing Du
Additional file 2: Figure S2. CISD2 expression endows the cellular resistance to ferroptosis inducer. (A–D) Analysis of the CISD2 expression by western blot in HT-1080 (A, C) or HL60 (B, D) cells, GAPDH was used as loading control; (E, F) Cell survival analysis in CISD2-modified HT-1080 cells after the treatment of Erastin (0–10 µM); (G, H) Cell survival analysis in CISD2-modified HL60 cells after the treatment of Erastin (0–15 µM); Cell survival in control cells...

Additional file 2 of Inhibition of CISD2 promotes ferroptosis through ferritinophagy-mediated ferritin turnover and regulation of p62–Keap1–NRF2 pathway

Yanchun Li, Bing Xu, Xueying Ren, Luyang Wang, Yaqing Xu, Yefeng Zhao, Chen Yang, Chen Yuan, Huanjuan Li, Xiangmin Tong, Ying Wang & Jing Du
Additional file 2: Figure S2. CISD2 expression endows the cellular resistance to ferroptosis inducer. (A–D) Analysis of the CISD2 expression by western blot in HT-1080 (A, C) or HL60 (B, D) cells, GAPDH was used as loading control; (E, F) Cell survival analysis in CISD2-modified HT-1080 cells after the treatment of Erastin (0–10 µM); (G, H) Cell survival analysis in CISD2-modified HL60 cells after the treatment of Erastin (0–15 µM); Cell survival in control cells...

Additional file 11 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 11 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 11: Figure S2. circPVT1 promotes the migration and invasion of NPC in vitroand metastasis of NPCin vivo. A-B. The expression of circPVT1 were measured in CNE2 and 5-8F cells after transfection with the circPVT1 overexpression vector or circPVT1 siRNA. PVT1 expression was not affected in CNE2 and 5-8F cells after overexpresion or knockdown of circPVT. All experiments were repeated at least three times. Data were represented as mean ± SD. ***, p <...

Additional file 12 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 12: Figure S3. circPVT1 promotes the migration and invasion of NPC cells through binding to β-TrCP. A. The secondary ion flow diagram of β-TrCP identified by mass spectrometry according to the peptide sequence (DFITALPAR) of β -TrCP.B. Schematic diagram of binding sites betweent circPVT1 and β-TrCP using the catRAPID website.The 230-280 nt of circPVT1 was predicted to bind to β-TrCP protein with high interaction score.C. The secondary structure of circPVT1 changed when 230-280...

Additional file 13 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 13: Figure S4. The WD40 repeat domain of β-TrCP interacts with circPVT1. A. The functional domain of the of β-TrCP proteins are illustrated (top), including the F-box domain and the WD40 domain. The expressing plasmids for full-length β-TrCP or truncated fragments (F-box and WD40) were constructed and confirmed using anti-Flag antibody. B. Wound healing assay showed that overexpression of β-TrCP inhibited NPC cells migration. Data were represented as mean ± SD. ***, p...

Additional file 14 of Circular RNA circPVT1 promotes nasopharyngeal carcinoma metastasis via the β-TrCP/c-Myc/SRSF1 positive feedback loop

Yongzhen Mo, Yumin Wang, Yian Wang, Xiangying Deng, Qijia Yan, Chunmei Fan, Shuai Zhang, Shanshan Zhang, Zhaojian Gong, Lei Shi, Qianjin Liao, Can Guo, Yong Li, Guiyuan Li, Zhaoyang Zeng, Weihong Jiang, Wei Xiong & Bo Xiang
Additional file 14: Figure S5. c-Myc is ubiquitinated substrate of β-TrCP. A. The expression of β-TrCP was examined in CNE2 and 5-8F cells after overexpression or knockdown of circPVT1 using western blotting.B. The β-TrCP binding proteins were identified in CNE2 cells by immunoprecipitation followed by LC-MS/MS.C. The interaction of c-Myc and β-TrCP was predicted by Molecular Docking ( http://hdock.phys.hust.edu.cn/ ). The blue part is the WD40 repeat domain of β-TrCP.D. The expression of c-Myc was...

Additional file 5 of Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles

Kohei Yuyama, Hui Sun, Yasuyuki Igarashi, Kenji Monde, Takumi Hirase, Masato Nakayama & Yoichi Makino
Additional file 5: Supplemental Fig. 5. Uncropped membrane images of western blot analysis of the APP Tg serum-derived EVs.

Additional file 2 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 2: Figure S2. Genes expression levels in lung tissues of neonatal mice (A) The mRNA expression of differentially expressed genes analysed using RT-qPCR in lung tissues of neonatal mice. B, C Nr4a1 expression analysed using western blot in lung tissues of neonatal mice. D Serpine1 expression in lung tissues of neonatal mice detected using RT-qPCR on P1, P7, and P21. E, F Serpine1 expression analysed using RT-qPCR and western blot in siRNA-treated neonatal...

Additional file 3 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 3: Figure S3. The effect of Serpine1 overexpression in lung epithelial cells. A Serpine1 expression analysed using RT-qPCR in Serpine1-overexpressing MLE-12 cells. B–D Serpine1 expression analysed using RT-qPCR, western blot and Immunofluorescence in Serpine1-overexpressing A549 cells. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing A549 cells. G p16 expressions analysed using western blot in Serpine1-overexpressing BEAS-2B cells. Data show mean ± SEM; Data was analysed using unpaired t-tests.

Additional file 3 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 3: Figure S3. The effect of Serpine1 overexpression in lung epithelial cells. A Serpine1 expression analysed using RT-qPCR in Serpine1-overexpressing MLE-12 cells. B–D Serpine1 expression analysed using RT-qPCR, western blot and Immunofluorescence in Serpine1-overexpressing A549 cells. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing A549 cells. G p16 expressions analysed using western blot in Serpine1-overexpressing BEAS-2B cells. Data show mean ± SEM; Data was analysed using unpaired t-tests.

Additional file 4 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 4: Figure S4. Enrichment of differentially expressed genes in Serpine1-overexpressing MLE-12 cells. A, B GO and KEGG enrichment of the upregulated genes. C, D GO and KEGG enrichment of the downregulated genes. Q-value ≤ 0.05 and fold-change ≥ 2 were used as the thresholds for screening the differentially expressed genes.

Additional file 5 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 5: Figure S5. The expression of DNA methylation related enzymes in lung tissues and TNF-α-treated MLE-12 cells. A Dnmt1, Dnmt3a, Mbd1, and Tet1 expression analysed using RT-qPCR in lung tissues of neonatal mice. B TNF-α expression analysed using RT-qPCR in lung tissues of neonatal mice. C Dnmt1, Dnmt3a, Mbd1, and Tet1 expression analysed using RT-qPCR in TNF-α-treated MLE-12 cells. D Serpine1 expression analysed using RT-qPCR in TNF-α-treated MLE-12 cells. Data show mean ±...

Additional file 7 of Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Dongting Yao, Jiuru Zhao, Qianqian Zhang, Tao Wang, Meng Ni, Sudong Qi, Qianwen Shen, Wei Li, Baihe Li, Xiya Ding & Zhiwei Liu
Additional file 7: Figure S7. Schematic representation of the molecular mechanisms underlying IUI-induced lung injury in neonatal mice.

Additional file 6 of Integrated assessment of the clinical and biological value of ferroptosis-related genes in multiple myeloma

Bibo Fu, Ruonan Shao, Huizhong Wang, Guanjun Chen, Shenrui Bai & Hua Wang
Additional file 6: Figure S5. Polydatin and dicoumarol sensitized MM cell lines to doxorubicin. Cell viabilities measured by CCK8 assay after MM cell lines treated with (A) doxorubicin or doxorubicin plus 60uM polydatin; (B) doxorubicin or doxorubicin plus 30uM dicoumarol for 48h.

Registration Year

  • 2022
    831

Resource Types

  • Image
    831

Affiliations

  • Huazhong University of Science and Technology
    831
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    553
  • Fudan University
    499
  • Chinese Academy of Sciences
    477
  • Sichuan University
    462
  • Zhejiang University
    453
  • Nanjing Medical University
    453
  • Capital Medical University
    451
  • Shanghai Jiao Tong University
    437
  • Sun Yat-sen University
    427