To identify the association between the kinetics of viral load and clinical outcome in severe coronavirus disease 2019 (COVID-19) patients, a retrospective study was performed by involved 188 hospitalized severe COVID-19 patients in the LOTUS China trial. Among the collected 578 paired throat swab (TS) and anal swab (AS) samples, viral RNA was detected in 193 (33.4%) TS and 121 (20.9%) AS. A higher viral RNA load was found in TS than that of AS,...

Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

Long non-coding RNA (lncRNA) ZFAS1 (zinc finger antisense 1) was demonstrated to play critical roles in various cancer progression. However, the functions of ZFAS in cervical cancers (CC) are unclear. Human CC cell lines were used for in vitro experiments. RT-qPCR (Real Time Quantitative PCR) was performed to detect the expression of ZFAS1, microRNA-190a-3p (miR-190a-3p) and Kruppel-like factor 6 (KLF6). Cell proliferation, invasion and migration assays were used to investigate biological behaviors of CC cells...

Additional file 2: Fig. S1. The negative control of flow cytometry analysis.

Additional file 2: Fig. S1. The negative control of flow cytometry analysis.

Additional file 3: Fig. S2. ADM was upregulated in glioma tissues based on online datasets and clinical collected samples. (A) GSE4412; (B) GSE4290; (C) overall survival of ADM according to CGGA dataset; (D) overall survival of ADM according to TCGA-GBMLGG dataset; (E and F) The mRNA and protein levels of ADM in glioma samples and non-cancerous peritumoral brain edema tissue.

Additional file 6: Figure S2. (A, F, K) Comparison of the relationship between the clinical characteristics of patients between high- and low-risk groups in the training, testing, and entire sets, respectively. (B–E) The scatter diagram showed the relationship between gender (B), clinical stage (C), T stage (D), N stage (E) and the risk score in the training set. (G–J) The scatter diagram showed the relationship between gender (G), clinical stage (E), T stage (F), N...

Additional file 4: Figure S4. (A) Analysis of NQO1 expression by RT-PCR in CISD2 silenced HT-1080 cells with or without the treatment of erastin; (B) Quantitative analysis of NQO1 expression in Fig. 4A;★P < 0.05 and ★★P < 0.01 between the indicated groups.

Additional file 12: Figure S6. Widespread occurrence of rumen bacteria in the communication network. (A) The occurrence of rumen bacteria in the network. (B) The occurrence of bacteria that synthesize AI-2 at the phylum level. (C) The occurrence of bacteria that synthesize and sense AI-2 at the phylum level. (D) The occurrence of bacteria that sense AI-2 at the phylum level. Numbers indicate the numbers of bacterial genomes in which the corresponding functions were found...

Supplemental material, sj-tiff-3-tah-10.1177_20406207221146031 for Antihuman T lymphocyte porcine immunoglobulin combined with cyclosporine as first-line immunosuppressive therapy for severe aplastic anemia in China: a large single-center, 10-year retrospective study by Wenrui Yang, Xu Liu, Xin Zhao, Li Zhang, Guangxin Peng, Lei Ye, Kang Zhou, Yuan Li, Jianping Li, Huihui Fan, Yang Yang, Youzhen Xiong, Liping Jing and Fengkui Zhang in Therapeutic Advances in Hematology

Additional file 4: Figure S4. Forest plot for failure to achieve clinical response (100-point response) at 4 weeks subgroup by previous TNF-α exposure in ADA and control groups.

Additional file 4: Figure S4. Forest plot for failure to achieve clinical response (100-point response) at 4 weeks subgroup by previous TNF-α exposure in ADA and control groups.

Additional file 1: Supplementary Fig. 1. Expression patterns of reference genes in different developmental stages of porcine oocytes and IVF embryos. Relative abundance of the reference genes GAPDH (A), B2M (B), H2A (C) and ACTB (D), in porcine oocytes and early IVF embryos. Data are presented as the mean ± standard deviation. *, P < 0.05; **, P < 0.01 between groups, as indicated.

Additional file 1: Supplementary Fig. 1. Expression patterns of reference genes in different developmental stages of porcine oocytes and IVF embryos. Relative abundance of the reference genes GAPDH (A), B2M (B), H2A (C) and ACTB (D), in porcine oocytes and early IVF embryos. Data are presented as the mean ± standard deviation. *, P < 0.05; **, P < 0.01 between groups, as indicated.

Additional file 3: Correlation between mRNA expression and methylation level of other genes.

Additional file 1: Figure S1. The increase rate of frequency by slope from low to high. The increase rate of frequency was defined as the frequency at a specific slope was divided by the accumulated frequency at all observed slopes that were less than the specific slope.

Efficacy and safety of nivolumab for advanced/recurrent non-small-cell lung cancer: an up-to-date meta-analysis of large-scale phase III randomized controlled trials Supplementary figure 1

Additional file 1: Figure S2. Sorting of CD4+ T cells in CD4+CD25+Foxp3+Treg cells and CD3+CD8− cells in CD3+CD8−IL-17+Th17 cells analyzed by flow cytometry. Fig. S2 is related to Fig. 5A.

Additional file 2 Supplementary Fig. 2. A phylogenetic tree demonstrates the genetic distance among all mouse KRAB-Zfp genes. The detailed description is the same as that in Fig. 1B.

Additional file 1: Figure S3. Propofol inhibited invasion and migration of lung cancer cells. A Effect of different doses (0.3 μg/mL, 3 μg/mL, 30 μg/mL) of propofol on lung cancer cell viability was measured by CCK-8 assay; B Cell invasion were detected using Transwell assay; C Cell migration was detected Transwell assay; D Positive rate of E-cadherin protein was detected by immunocytochemistry and the stronger red fluorescence meant the higher positive rate. All experiments were...

Previously, we reported that the mediation of Newcastle disease virus (NDV) pathogenicity by the 524YLMY527 motif depends mainly on the regulation of F protein transport to the cell surface. The virus and host determinants that govern this intracellular trafficking remain unknown. Here, we confirmed that host adaptor protein (AP) complexes are involved in NDV infection using small interfering RNA. The transport of viral F protein to the cell surface depends on host transport proteins. We...

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