Supplemental material, sj-eps-2-tam-10.1177_17588359221133546 for Circulating tumour DNA biomarkers in savolitinib-treated patients with non-small cell lung cancer harbouring MET exon 14 skipping alterations: a post hoc analysis of a pivotal phase 2 study by Yongfeng Yu, Yongxin Ren, Jian Fang, Lejie Cao, Zongan Liang, Qisen Guo, Sen Han, Zimei Ji, Ye Wang, Yulan Sun, Yuan Chen, Xingya Li, Hua Xu, Jianying Zhou, Liyan Jiang, Ying Cheng, Zhigang Han, Jianhua Shi, Gongyan Chen, Rui Ma, Yun Fan,...

Additional file 4: Supplementary figure 4. Efficiency of miR-185-3p transfection and functional enrichment analyses of target genes of circ_0002078. (A) Efficiency verification of miR-185-3p overexpression in MEC-1 cells by qRT-PCR. (B) GO analysis results demonstrated that the target genes of circ_0002078 were enriched in positive regulation of gene expression, lymphocyte proliferation, and regulation of B cell apoptotic process. (C) KEGG pathway analysis showed that the target genes were primarily relevant to JAK-STAT signaling pathway and...

Additional file 1: Supplementary Figure 1.

Additional file 2: Supplementary Figure 2.

Additional file 5: Supplementary Figure 5.

Additional file 9: Figure S4. Cis-acting elements within the promoters of CsFLS2 and CsFLS3.

Additional file 1: Supplementary Figure 1. Skin lesions of patient 1 before (A) and after (B) the 12-week TNFi treatment. The severe acne characterized by cyst nodules and abscesses in the cheeks improved after the treatment. The area of the acne shrank.

Additional file 2: Supplementary Figure 2. Skin lesions of patient 2 before (A) and after (B) the 12-week TNFi treatment. The severe acne characterized by cyst nodules and abscesses in the forehead and nose improved after the treatment, left only some slight scar formation.

Additional file 2: Supplementary Figure 2. Skin lesions of patient 2 before (A) and after (B) the 12-week TNFi treatment. The severe acne characterized by cyst nodules and abscesses in the forehead and nose improved after the treatment, left only some slight scar formation.

Additional file 5: Figure S2. Median-joining network of Pv230 among eight geographically diverse populations. Haplotypes composed of nucleotide polymorphism in ICP of Pv230 with a frequency > 1 were used to create a median-joining network. Each node represents one haplotype, node size indicates haplotype frequency, and node color corresponds to the country of origin. Line length is proportional to genetic distance.

Additional file 3 Supplementary Fig. 3. The combined features about the zinc fingers (ZnFs) for all the mouse KRAB-ZFPs, including the composition specificity and frequency of occurrence. The detailed description is the same as that in Fig. 1C. Each row represents a single KRAB-ZFP.

Additional file 6. The ML inference of ancestral sequence by the Maximum likelihood method in PAML software. The reconstructed ancestral states of mammalian species are highlighted with larger red letters.

Additional file 6. The ML inference of ancestral sequence by the Maximum likelihood method in PAML software. The reconstructed ancestral states of mammalian species are highlighted with larger red letters.

Additional file 8. The population RNAseq expression quantification of IRF9. The boxplots show the expression levels within different tissues/organs/cells.

Additional file 9. The UCSC mapping of Denisovan and Neanderthal reads to the human genome. The red arrows show the DNA substitutions in Denisovan and Neanderthal reads. The amino acid Val129, identical among all three Homo species, is boxed.

Additional file 7 Supplementary Fig. 7. ZnF fingerprints analyses for human KRAB-ZFPs on chr19. The combined features about the ZnF fingerprints for KRAB-ZFPs for all the human KRAB-ZFPs on chromosome 19. The detailed description is the same as that in Fig. 1C. Each row represents a single KRAB-ZFP.

Additional file 1.

Supplementary Material 6

Supplementary Material 7

Additional file 4: Fig. S4. RT-qPCR analysis for SOX17, BLIMP1, TFAP2C, NANOS3, POU5F1, SOX2 during hPGC differentiation in day 4 embryoids, Related to Fig. 1; n = 3 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by Student’s t-test (two-sided), compared to WT group, *p < 0.05, **p < 0.01, ***p < 0.001, Related to Fig. 1.

Additional file 7: Fig. S7. Transcription and DNA methylation Profile of Each Cell Lines, Related to Fig. 6. (A) Unsupervised hierarchical clustering of the transcriptomes (two independent experiments) of each sample, AU (Approximately Unbiased) p-value and BP (Bootstrap Probability) value; (B) Hierarchical clustering on the top changed 1000 promoters by RnBeads; (C) Heat map of key PGC-associated genes (early and late), pluripotency, mesoderm, endoderm, and gonadal somatic (Soma) markers; (D) Violin plots showing distribution of...

Additional file 1: Supplementary Fig. 1. Study flowchart. Abbreviations: SCC, esophageal squamous cell carcinoma; CRT, chemoradiotherapy.

Additional file 6: Fig. S6. Single-cell sequencing analysis of adult mice intestine. a Dotplot for highest specificity gene markers of cell clusters. Dot size represents the percentage of cells expressing the denoted gene, and the color represents average normalized expression level within the denoted cluster. b The expression of GFAP in different cell clusters by analysis of the Uniform Manifold Approximation and Projection (UMAP). c Different cell subpopulation of the GSE156905 dataset derived from the...

To compare the efficacies between ultrasound-guided curettage combined with hysteroscopic electro-resection after injection of pituitrin and hysteroscopic electro-resection after methotrexate chemotherapy in the treatment of caesarean scar pregnancy (CSP). 60 patients with CSP admitted to our hospital were selected as the study subjects, and divided into observation group (n = 35) and control group (n = 25) in accordance with the therapeutic options. The observation group was treated with ultrasound-guided curettage combined with hysteroscopic electro-resection...

Additional file 1: Supplementary Figure 1. Kaplan-Meiercurves of OS differences stratified by age, gender, tumor grade and stagebetween both groups in the entire TCGA set.