828 Works

Circular RNA circCHFR downregulation protects against oxidized low-density lipoprotein-induced endothelial injury via regulation of microRNA-15b-5p/growth arrest and DNA damage inducible gamma

Yang Li & Bing Wang
Atherosclerosis is the leading cause of coronary heart disease. In recent years, circ_0029589 (circCHFR) has been found to be associated with atherosclerosis development. However, the molecular mechanism of circCHFR action in atherosclerosis development is unknown. This study was aimed to investigate the function and action mechanism of circCHFR in atherosclerosis development. An atherosclerosis cell model was created by exposing human vascular endothelial cells (HUVECs) to oxidized low-density lipoprotein. The expression of circCHFR, microRNA(miR)-15b-5p, growth arrest...

Melatonin affects hypoxia-inducible factor 1α and ameliorates delayed brain injury following subarachnoid hemorrhage via H19/miR-675/HIF1A/TLR4

Zhijian Xu, Fengduo Zhang, Hu Xu, Fan Yang, Gezhi Zhou, Minfeng Tong, Yaqiang Li & Song Yang
This study aimed to investigate the molecular mechanism of how melatonin (MT) interferes with hypoxia-inducible factor 1α (HIF1A) and toll-like receptor 4 (TLR4) expression, which is implicated in the management of delayed brain injury (DBI) after subarachnoid hemorrhage (SAH). Luciferase assay, real-time PCR, Western-blot analysis and immunohistochemistry (IHC) assays were utilized to explore the interaction among H19, miR-675, HIF1A and TLR4, and to evaluate the effect of MT on the expression of above transcripts in...

Hyperthermia inhibits growth of nasopharyngeal carcinoma through degradation of c-Myc

Xiaole Li, Shichao Duan, Yingjuan Zheng, Yongqiang Yang, Lei Wang, Xinqiang Li, Qing Zhang, Rick F. Thorne, Wencai Li & Daoke Yang
Hyperthermia is a widely used adjunct treatment for different cancers including nasopharyngeal carcinoma (NPC). The protooncogene c-Myc is up-regulated in NPC and its expression is associated with poor prognosis. We hypothesized that c-Myc constitutes an important hyperthermia treatment target, and we investigated its contribution to hyperthermia responses in NPC. The growth of the human NPC cell lines CNE1 and CNE2 was analyzed using CCK-8 and clonogenicity assays after 43 °C hyperthermia, knockdown or overexpression of...

Additional file 1 of ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Jing Li, Haoran Du, Xin Ji, Yihan Chen, Yishuai Li, Boon Chin Heng & Jianguang Xu
Additional file 1: Figure S1. The mRNA level of CBFA1 in qRT-PCR (a) and RNA-Seq (b). *P < 0.05.

Additional file 4 of ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Jing Li, Haoran Du, Xin Ji, Yihan Chen, Yishuai Li, Boon Chin Heng & Jianguang Xu
Additional file 4: Figure S4. HE (scale bar = 50 μm) and Masson (scale bar = 50 μm) staining were performed to evaluate ectopic bone formation in nude mice (n=4).

Knockdown of long non-coding RNA plasmacytoma variant translocation 1 relieves ox-LDL-induced endothelial cell injury through regulating microRNA-30c-5p in atherosclerosis

Geng Li, Wenxia Zong, Lei Liu, Juan Wu & Jing Pang
Atherosclerosis (AS) is a chronic inflammatory disease involving endothelial dysfunction, and is one of the main causes of death from cardiovascular disease (CVD). Long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) is overexpressed in the serum of CVD patients. However, the mechanism by which lncRNA PVT1 functions in AS remains unknown. Our research was designed to probe interactions involving lncRNA PVT1 and oxidized low-density lipoprotein (ox-LDL)-stimulated endothelial cell injury in AS. lncRNA PVT1 expression...

Knockdown of long non-coding RNA plasmacytoma variant translocation 1 relieves ox-LDL-induced endothelial cell injury through regulating microRNA-30c-5p in atherosclerosis

Geng Li, Wenxia Zong, Lei Liu, Juan Wu & Jing Pang
Atherosclerosis (AS) is a chronic inflammatory disease involving endothelial dysfunction, and is one of the main causes of death from cardiovascular disease (CVD). Long non-coding RNA plasmacytoma variant translocation 1 (lncRNA PVT1) is overexpressed in the serum of CVD patients. However, the mechanism by which lncRNA PVT1 functions in AS remains unknown. Our research was designed to probe interactions involving lncRNA PVT1 and oxidized low-density lipoprotein (ox-LDL)-stimulated endothelial cell injury in AS. lncRNA PVT1 expression...

Additional file 4 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 4: Figure S1. Validation of ω-transaminase activity based on color reaction. 1: Control 2: Empty vector plasmid strain breaking solution 3: Breaking solution of strain expressing He-ωTA protein 4: Breaking solution of strain expressing Cv-ωTA protein 5: Purified He-ωTA protein solution 6: Purified Cv-ωTA protein solution.

Additional file 5 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 5: Figure S2. Intracellular NADH content of MER-oE strains under different medium conditions. Data represent mean ± S.D. (error bars) from three independent experiments.

Additional file 6 of Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Qi Wang, Linlin Ma, Zhiguo Wang, Quan Chen, Qian Wang & Qingsheng Qi
Additional file 6: Figure S3. The petP and petE plasmids expression in E. coil by SDS-PAGE analysis of whole cell (WC), supernatant (S), and protein marker (M).

Knockdown of lectin-like oxidized low-density lipoprotein-1 ameliorates alcoholic cardiomyopathy via inactivating the p38 mitogen-activated protein kinase pathway

Yifan Zhang, Ruiqi Zhang, Lan Lu, Na Zhou, Xiaoyan Lv, Xin Wang & Zhanbin Feng
LOX-1 triggers myocardial fibrosis, but its roles and mechanisms in alcoholic cardiomyopathy and the involvement of the downstream signaling pathways had not been fully reported. We planned to explore how LOX-1 facilitated myocardial fibrosis in alcoholic cardiomyopathy. The in vitro and in vivo alcoholic cardiomyopathy model was established by alcohol treatment to rats’ cardiac fibroblasts and rats, respectively. Masson staining was conducted to observe the collagen deposition and the IHC assay was executed to evaluate...

Additional file 2 of Combining QTL mapping and RNA-Seq Unravels candidate genes for Alfalfa (Medicago sativa L.) leaf development

Xueqian Jiang, Xijiang Yang, Fan Zhang, Tianhui Yang, Changfu Yang, Fei He, Ting Gao, Chuan Wang, Qingchuan Yang, Zhen Wang & Junmei Kang
Additional file 2: Fig. S2 Leaf- related QTLs on 32 linkage groups from a genetic linkage map of maternal parent (P2).

Additional file 4 of Identification of pyroptosis-related genes and long non-coding RNAs signatures in osteosarcoma

Jian Zhang, Jianjian Deng, Rui Ding, Jinghong Yuan, Jiahao Liu, Xiaokun Zhao, Tianlong Wu, Jingyu Jia & Xigao Cheng
Additional file 4: Fig. S2. The PPI network.

Additional file 1 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 1: Figure S1. (A) WT-HeLa cells were treated with 20 μM of J3 for 3, 6, and 12 h for western blot, quantification of the protein expression of p62 (B) and LC3 (C) was analyzed. (D) GFP-LC3-HeLa were treated as (A), GFP-LC3 dots were analyzed. Scale bar = 20 μm. (E) GFP-LC3 dots of (D) were quantified. (F) GFP-LC3-MEF and GFP-LC3-A549 cells were incubated with 20 μM of J3 for 6 h. GFP-LC3...

Additional file 2 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 2: Figure S2. WT-HeLa cells were incubated with 20 μM of J3 with or without 2 mM of NAC, 1 μM of compound C, 10 μM of U0126, 10 μM of BAPTA for 6 h, respectively. The protein level of LC3-II was detected by western blot and quantified.

Additional file 5 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 5: Figure S5. (A) The flow diagram of the mice generation, genotype identification, and the administration methods (HdhQ140/Q140 and WT mice are not littermates in our study). (B) Dissection of the mice striatum were shown in the picture, the red line indicates the outline for a piece of striatum. (C) The immunostaining of T-HTT (anti-HTT, MAB2166) in the striatum of WT and homozygous mice was analyzed at 12-month-old. (D) The expression of total...

Additional file 5 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 5: Figure S5. (A) The flow diagram of the mice generation, genotype identification, and the administration methods (HdhQ140/Q140 and WT mice are not littermates in our study). (B) Dissection of the mice striatum were shown in the picture, the red line indicates the outline for a piece of striatum. (C) The immunostaining of T-HTT (anti-HTT, MAB2166) in the striatum of WT and homozygous mice was analyzed at 12-month-old. (D) The expression of total...

Additional file 6 of Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Jiahui Long, Xia Luo, Dongmei Fang, Haikun Song, Weibin Fang, Hao Shan, Peiqing Liu, Boxun Lu, Xiao-Ming Yin, Liang Hong & Min Li
Additional file 6: Figure S6. (A-B) Analysis of the expression level of T-HTT (anti-HTT, MAB2166) and DARPP32 in the striatum of 15-month-old homozygous HdhQ140/Q140 mice with or without 60 mg/kg of J3 administration for 3 months. Data are presented as mean ± sem from three individual samples. *p < 0.05, **p < 0.01, ***p < 0.001. (C) Values of parameters characterizing physicochemical and basic ADME properties calculated using ACD/Percepta.

sj-tif-3-onc-10.1177_11795549221127161 – Supplemental material for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery

Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang & Rui Yuan
Supplemental material, sj-tif-3-onc-10.1177_11795549221127161 for Effect of Number of Retrieved Lymph Nodes on Prognosis in FIGO Stage IA1-IIA2 Cervical Cancer Patients Treated With Primary Radical Surgery by Shan Jiang, Peng Jiang, Tingting Jiang, Yuan Tu, Jingni Zhang, Ning Li, Wei Kong, Yuzhen Huang and Rui Yuan in Clinical Medicine Insights: Oncology

Additional file 1 of Characterization of pyruvate metabolism and citric acid cycle patterns predicts response to immunotherapeutic and ferroptosis in gastric cancer

Xu Wang, Bing Xu, Jing Du, Jun Xia, Guojie Lei, Chaoting Zhou, Jiayu Hu, Yinhao Zhang, Sufeng Chen, Fangchun Shao, Jiyun Yang & Yanchun Li
Additional file 1: Figure S1. The relative abundance of P-CA regulators in DLAT wild and DLAT mutation groups is presented

Additional file 3 of TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway

Rong Li, Jing Zhang, Qiong Wang, Meng Cheng & Bin Lin
Additional file 3: Figure S3. TPM1 knockdown reduces LPS-induced inflammation and function decline in C57BL/6J mice. A, B mRNA level of TPM1 (A) and IL-10 (B) in C57BL/6 J mouse retinas after treatments with LPS and siCTR or siTPM1-1. Data are presented as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparison test (compared to LPS + siCTR or LPS + siTPM1-1, *p < 0.05, **p < 0.01). n = 4 or...

Additional file 7 of CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice

Hongrui Jiang, Yan Ren, Junyao Guo, Huijie Yang, Xiaotong Zhu, Wenhao Li, Liangzhi Tao, Yue Zhan, Qi Wang, Yuejin Wu, Binmei Liu & Yafeng Ye
Additional file 7: Figure S7. (A) Phylogenetic tree of CEF3 in rice and Arabidopsis. (B) The amino acid sequence alignment between the AtSCD2 and OsCEF3. Different rectangles represent different functional domains.

Additional file 2 of Biallelic mutations of TTC12 and TTC21B were identified in Chinese patients with multisystem ciliopathy syndromes

Weicheng Chen, Feifei Wang, Weijia Zeng, Xinyan Zhang, Libing Shen, Yuan Zhang & Xiangyu Zhou
Additional file 2: Fig S1. (A) Echocardiography shows transposition of great arteries and ventricular septal defect in the patient JM0087 (F-2: II-1). (B) Chest X-ray shows dextrocardia in the patient JM0529 (F-4:II-1) at the age of 2 years

sj-jpg-3-jbm-10.1177_03936155221132292 - Supplemental material for Development and validation of a nomogram involving immunohistochemical markers for prediction of recurrence in early low-risk endometrial cancer

Wei Kong, Yuan Tu, Peng Jiang, Yuzhen Huang, Jingni Zhang, Shan Jiang, Ning Li & Rui Yuan
Supplemental material, sj-jpg-3-jbm-10.1177_03936155221132292 for Development and validation of a nomogram involving immunohistochemical markers for prediction of recurrence in early low-risk endometrial cancer by Wei Kong, Yuan Tu, Peng Jiang, Yuzhen Huang, Jingni Zhang, Shan Jiang, Ning Li and Rui Yuan in The International Journal of Biological Markers

Additional file 4 of Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification

Changchuan Ye, Yuting Yang, Xi Chen, Lijie Yang, Xia Hua, Mengjie Yang, Xiangfang Zeng & Shiyan Qiao
Additional file 4: Fig S4. Schematic illustration of DSB induction and homologous recombination. After preparing competent cells, the pTarget-gene and Donor DNA which harbored homology arms (HRR and HRL) that targeted a chromosomal locus spanning the middle of targeted gene and the DSB site were electroporated into cells.

Registration Year

  • 2022
    828

Resource Types

  • Image
    828

Affiliations

  • Sichuan University
    828
  • Chinese Academy of Medical Sciences & Peking Union Medical College
    632
  • Fudan University
    518
  • Zhejiang University
    479
  • Shanghai Jiao Tong University
    477
  • Capital Medical University
    476
  • Huazhong University of Science and Technology
    462
  • Sun Yat-sen University
    461
  • Nanjing Medical University
    434
  • Chinese Academy of Sciences
    419